Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia
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AGUS WIDODO
https://orcid.org/0000-0003-3255-6188
MIRNI LAMID
https://orcid.org/0000-0001-6539-4571
MUSTOFA HELMI EFFENDI
♥
https://orcid.org/0000-0001-9727-411X
WIWIEK TYASNINGSIH
https://orcid.org/0000-0003-0604-8534
WURLINA
https://orcid.org/0000-0002-1307-3845
MOHAMMAD ANAM AL-ARIF
https://orcid.org/0000-0002-5220-9311
DADIK RAHARJO
https://orcid.org/0000-0002-3032-188X
SOEHARSONO
https://orcid.org/0000-0002-9634-6414
ASWIN RAFIF KHAIRULLAH
https://orcid.org/0000-0001-9421-9342
KATTY HENDRIANA PRISCILIA RIWU
https://orcid.org/0000-0003-0395-2296
LITA RAKHMA YUSTINASARI
https://orcid.org/0000-0003-3308-5365
SHENDY CANADYA KURNIAWAN
https://orcid.org/0000-0003-0470-3363
OTTO SAHAT MARTUA SILAEN
https://orcid.org/0000-0002-5170-6797
MOSES IKECHUKWU BENJAMIN
https://orcid.org/0000-0002-5928-8198
DANIAH ASHRI AFNANI
https://orcid.org/0000-0003-0719-6061
Abstract
Abstract. Widodo A, Lamid M, Effendi MH, Tyasningsih W, Wurlina, Al-Arif MA, Raharjo D, Soeharsono, Khairullah AF, Riwu KHP, Yustinasari LR, Kurniawan SC, Silaen OSM, Benjamin MI, Afnani DA. 2023. Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia. Biodiversitas 24: 1900-1907. Escherichia coli bacteria initially reside in the digestive tract of humans and animals. This bacterium is treated with antibiotics, but this treatment may trigger antibiotic resistance. Antibiotic resistance genes found in E. coli can transfer to other bacteria, especially in the case of the emergence of multidrug resistance (MDR) bacteria. E. coli is a significant environmental contaminant on dairy farms and is frequently linked to genes that encode extended-spectrum beta-lactamase. This research focused on investigating the potential hazards of multidrug resistance E. coli collected from wastewater on dairy farms in East Java, Indonesia. The number of research samples used was 139 wastewater samples collected from 125 dairy farms in East Java, Indonesia. First, wastewater samples from dairy farms were isolated into BGLB media, then continued with culture on EMBA media, and identifying E. coli bacteria with TSIA and IMViC biochemical tests. The resistance patterns of E. coli were obtained through disk diffusion antibiotic tests on tetracycline, streptomycin, trimethoprim, chloramphenicol, and aztreonam. The results of the isolation and identification obtained 122 (87.77%) E. coli isolates. Antimicrobial susceptibility test to E. coli isolates showed 14 (11.47%) MDR E. coli isolates. The antimicrobial drug resistance pattern TE-ST-W (tetracycline, streptomycin, trimethoprim) accounted for most MDR E. coli isolates in this study, comprising 10 (36.26%); followed by TE-ST-C (tetracycline, streptomycin, chloramphenicol) with two (6.54%) MDR E. coli and two (6.54%) MDR E.coli isolates were resistant to four antibiotics, with the pattern of resistance to TE-ST-W-C (tetracycline, streptomycin, trimethoprim, chloramphenicol) antibiotic. The discovery of multidrug-resistant E. coli isolated from wastewater on dairy farms in East Java, Indonesia, is a new potential hazard that affects public health and needs solutions. One Health integration is anticipated to be used as a solution.
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