Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Yanestria SM, Dameanti FNAEP, Musayannah BG, Pratama JWA, Witaningrum AM, Effendi MH, Ugbo EN. 2022. Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia. Biodiversitas 23: 4460-4465. Escherichia coli is one of the bacteria that can be used as an indicator of environmental pollution. This bacterium has the ability to become an antimicrobial-resistant bacterium that impacts public health. The antimicrobial ability can develop into a bacterium that produces extended-spectrum ?-lactamase (ESBL). The purpose of this study was to reveal the occurrence of extended-spectrum ?-lactamase (ESBL) producing E. coli from a broiler farm environment in Pasuruan district and to describe the phenotypic pattern of E. coli producing ESBL that has been detected. A total of 175 samples were used in this study consisting of 115 samples of coop swabs and 65 samples of wastewater around the farm. The samples were isolated and identified to find E. coli by using different culture media viz. McConkey agar (MCA), eosin methylene blue agar (EMBA), Gram staining, indole test, methyl red Voges Proskauer (MR-VP), citrate, and triple sugar iron agar (TSIA). Detection of ESBL using the double disc synergy test (DDST) according to standard Clinical and Laboratory Standards Institute procedures and the VITEK®2 compact apparatus. The results of ESBL confirmation with DDST and VITEK®2 showed that 16 (9.14%) of the 175 environmental samples confirmed E. coli produced ESBL. The results of the VITEK®2 test also produced a phenotypic pattern of resistance properties of ESBL-producing E. coli and found 12 types of resistance patterns. The combination of “AM AMP ATM KZ CTX CRO CIP” and “AM AMP ATM KZ CTX CRO GM CIP SXT” are the 2 most common resistance patterns (18.75%), while the other 10 resistance patterns occur at the same level (6.25%). The data presented here confirmed the presence of ESBL-producing E. coli in the farm environment, which can contribute to the dissemination of MDR bacteria in the environment if not monitored. Therefore, the presence of ESBL-producing E. coli in Pasuruan is worrisome since it can lead to an impact on human health.
##plugins.themes.bootstrap3.article.details##
Most read articles by the same author(s)
- DIAN AYU PERMATASARI, ADIANA MUTAMSARI WITANINGRUM, FRESHINTA JELLIA WIBISONO, MUSTOFA HELMI EFFENDI, Detection and prevalence of multidrug-resistant Klebsiella pneumoniae strains isolated from poultry farms in Blitar, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 10 (2020)
- MUSTOFA HELMI EFFENDI, WIWIEK TYASNINGSIH, YEMIMA ANGGUN YURIANTI, JOLA RAHMAHANI, NENNY HARIJANI, HANI PLUMERIASTUTI, Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 1 (2021)
- SANCAKA CHASYER RAMANDINIANTO, ASWIN RAFIF KHAIRULLAH, MUSTOFA HELMI EFFENDI, MecA gene and methicillin-resistant Staphylococcus aureus (MRSA) isolated from dairy farms in East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 8 (2020)
- RIBBY ANSHARIETA, SANCAKA CHASYER RAMANDINIANTO, MUSTOFA HELMI EFFENDI, HANI PLUMERIASTUTI, Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 4 (2021)
- FRESHINTA JELLIA WIBISONO, BAMBANG SUMIARTO, TRI UNTARI, MUSTOFA HELMI EFFENDI, DIAN AYU PERMATASARI, ADIANA MUTAMSARI WITANINGRUM, Short Communication: The presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli on layer chicken farms in Blitar Area, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 6 (2020)
- ASWIN RAFIF KHAIRULLAH, SRI AGUS SUDJARWO, MUSTOFA HELMI EFFENDI, SANCAKA CASHYER RAMANDINIANTO, MARIA AEGA GELOLODO, AGUS WIDODO, KATTY HENDRIANA PRISCILIA RIWU, DYAH AYU KURNIAWATI, SAIFUR REHMAN, Profile of Multidrug Resistance and Methicillin-Resistant Staphylococcus aureus (MRSA) on dairy cows and risk factors from farmer , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 6 (2022)
- FIDI NUR AINI EKA PUJI DAMEANTI, SHEILA MARTY YANESTRIA, AGUS WIDODO, MUSTOFA HELMI EFFENDI, HANI PLUMERIASTUTI, WIWIEK TYASNINGSIH, RAHAYU SUTRISNO, M. ALI AKRAMSYAH, Incidence of Escherichia coli producing Extended-spectrum beta-lactamase in wastewater of dairy farms in East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 2 (2023)
- MUSTOFA HELMI EFFENDI, HAYYUN DURROTUL FARIDAH, FRESHINDY MARISSA WIBISONO, FRESHINTA JELLIA WIBISONO, NABILATUN NISA, FATIMAH, EMMANUEL NNABUIKE UGBO, Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 7 (2022)