Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia
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AGUS WIDODO
https://orcid.org/0000-0003-3255-6188
MIRNI LAMID
https://orcid.org/0000-0001-6539-4571
MUSTOFA HELMI EFFENDI
♥
https://orcid.org/0000-0001-9727-411X
WIWIEK TYASNINGSIH
https://orcid.org/0000-0003-0604-8534
WURLINA
https://orcid.org/0000-0002-1307-3845
MOHAMMAD ANAM AL-ARIF
https://orcid.org/0000-0002-5220-9311
DADIK RAHARJO
https://orcid.org/0000-0002-3032-188X
SOEHARSONO
https://orcid.org/0000-0002-9634-6414
ASWIN RAFIF KHAIRULLAH
https://orcid.org/0000-0001-9421-9342
KATTY HENDRIANA PRISCILIA RIWU
https://orcid.org/0000-0003-0395-2296
LITA RAKHMA YUSTINASARI
https://orcid.org/0000-0003-3308-5365
SHENDY CANADYA KURNIAWAN
https://orcid.org/0000-0003-0470-3363
OTTO SAHAT MARTUA SILAEN
https://orcid.org/0000-0002-5170-6797
MOSES IKECHUKWU BENJAMIN
https://orcid.org/0000-0002-5928-8198
DANIAH ASHRI AFNANI
https://orcid.org/0000-0003-0719-6061
Abstract
Abstract. Widodo A, Lamid M, Effendi MH, Tyasningsih W, Wurlina, Al-Arif MA, Raharjo D, Soeharsono, Khairullah AF, Riwu KHP, Yustinasari LR, Kurniawan SC, Silaen OSM, Benjamin MI, Afnani DA. 2023. Potential hazards of multidrug resistance Escherichia coli collected from wastewater on dairy farms in East Java, Indonesia. Biodiversitas 24: 1900-1907. Escherichia coli bacteria initially reside in the digestive tract of humans and animals. This bacterium is treated with antibiotics, but this treatment may trigger antibiotic resistance. Antibiotic resistance genes found in E. coli can transfer to other bacteria, especially in the case of the emergence of multidrug resistance (MDR) bacteria. E. coli is a significant environmental contaminant on dairy farms and is frequently linked to genes that encode extended-spectrum beta-lactamase. This research focused on investigating the potential hazards of multidrug resistance E. coli collected from wastewater on dairy farms in East Java, Indonesia. The number of research samples used was 139 wastewater samples collected from 125 dairy farms in East Java, Indonesia. First, wastewater samples from dairy farms were isolated into BGLB media, then continued with culture on EMBA media, and identifying E. coli bacteria with TSIA and IMViC biochemical tests. The resistance patterns of E. coli were obtained through disk diffusion antibiotic tests on tetracycline, streptomycin, trimethoprim, chloramphenicol, and aztreonam. The results of the isolation and identification obtained 122 (87.77%) E. coli isolates. Antimicrobial susceptibility test to E. coli isolates showed 14 (11.47%) MDR E. coli isolates. The antimicrobial drug resistance pattern TE-ST-W (tetracycline, streptomycin, trimethoprim) accounted for most MDR E. coli isolates in this study, comprising 10 (36.26%); followed by TE-ST-C (tetracycline, streptomycin, chloramphenicol) with two (6.54%) MDR E. coli and two (6.54%) MDR E.coli isolates were resistant to four antibiotics, with the pattern of resistance to TE-ST-W-C (tetracycline, streptomycin, trimethoprim, chloramphenicol) antibiotic. The discovery of multidrug-resistant E. coli isolated from wastewater on dairy farms in East Java, Indonesia, is a new potential hazard that affects public health and needs solutions. One Health integration is anticipated to be used as a solution.
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References
Ahsan A, Rehman TAU, Irshad H, Shahzad MA, Siddique A, Jamil A, Ali A. 2022. Antibiotic resistance pattern and molecular detection of ESBL-associated genes in E. coli from surface and wastewater of Islamabad capital territory, Pakistan. J Water Health 20 (4): 601-609. DOI: 10.2166/wh.2022.216.
Anjum MF, Schmitt H, Börjesson S, Berendonk TU; WAWES network. 2021. The potential of using E. coli as an indicator for the surveillance of antimicrobial resistance (AMR) in the environment. Curr Opin Microbiol 64: 152-158. DOI: 10.1016/j.mib.2021.09.011.
Ansharieta R, Ramandinianto SC, Effendi MH, Plumeriastuti H. 2021. Molecular identification of bla CTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia. Biodiversitas 22 (4): 1600-1605. DOI: 10.13057/biodiv/d220402.
Ansharieta R, Effendi MH, Plumeriastuti H. 2020. Detection of multidrug-resistant (MDR) Escherichia coli isolated from raw milk in East Java Province, Indonesia. Indian J Forensic Med Toxicol 14 (4): 3403-3407. DOI: 10.37506/ijfmt.v14i4.12151.
Aslam N, Khan SU, Usman T, Ali T. 2021. Phylogenetic genotyping, virulence genes and antimicrobial susceptibility of Escherichia coli isolates from cases of bovine mastitis. J Dairy Res 88 (1): 78-79. DOI: 10.1017/S002202992100011X.
Ben W, Wang J, Cao R, Yang M, Zhang Y, Qiang Z. 2017. Distribution of antibiotic resistance in the effluents of ten municipal wastewater treatment plants in China and the effect of treatment processes. Chemosphere 172: 392-398. DOI: 10.1016/j.chemosphere.2017.01.041.
Bengtsson-Palme J, Kristiansson E, Larsson DGJ. 2018. Environmental factors influencing the development and spread of antibiotic resistance. FEMS Microbiol Rev 42 (1): fux053. DOI: 10.1093/femsre/fux053.
Calahorrano-Moreno MB, Ordoñez-Bailon JJ, Baquerizo-Crespo RJ, Duenas-Rivadeneira AA, Montenegro MCBSM, Rodríguez-Díaz JM. 2022. Contaminants in the cow's milk we consume? Pasteurization and other technologies in the elimination of contaminants. F1000Res 11: 91. DOI: 10.12688/f1000research.108779.1.
Catalano A, Iacopetta D, Ceramella J, Scumaci D, Giuzio F, Saturnino C, Aquaro S, Rosano C, Sinicropi MS. 2022. Multidrug resistance (MDR): a widespread phenomenon in pharmacological therapies. Molecules 27 (3): 616. DOI: 10.3390/molecules27030616.
Chaleshtori FS, Arani NM, Aghadavod E, Naseri A, and Chaleshtori RS. 2017. Molecular characterization of Escherichia coli recovered from traditional milk products in Kashan, Iran. Vet World 10 (10): 1264-1268. DOI: 10.14202/vetworld.2017.1264-1268.
Chitanand MP, Kadam TA, Gyananath G, Totewad ND, Balhal DK. 2010. Multiple antibiotic resistance indexing of coliforms to identify high risk contamination sites in aquatic environment. Indian J Microbiol 50: 216-220. DOI: 10.1007/s12088-010-0042-9.
CLSI. 2018. Performance Standards for Antimicrobial Susceptibility Testing. 28th ed. CLSI supplement M100. Clinical and Laboratory Standards Institute.
Dahms C, Hubner N-O, Kossow A, Mellmann A, Dittmann K, Kramer A. 2015. Occurrence of ESBL-producing Escherichia coli in livestock and farmworkers in Mecklenburg-Western Pomerania, Germany. PLoS One 10 (11): e0143326. DOI: 10.1371/journal.pone.0143326.
Decline V, Effendi MH, Rahmaniar RP, Yanestria SM, Harijani N. 2020. Profile of antibiotic-resistant and presence of methicillin-resistant Staphylococcus aureus from nasal swab of dogs from several animal clinics in Surabaya, Indonesia. Int J One Health 6 (1): 90-94. DOI: 10.14202/ijoh.2020.90-94.
Delgado-Blas JF, Ovejero CM, David S, Montero N, Calero-Caceres W, Garcillan-Barcia MP, de la Cruz F, Muniesa M, Aanensen DM, Gonzalez-Zorn B. 2021. Population genomics and antimicrobial resistance dynamics of Escherichia coli in wastewater and river environments. Commun Biol 4 (1): 457. DOI: 10.1038/s42003-021-01949-x
Dhingra S, Rahman NAA, Peile E, Rahman M, Sartelli M, Hassali MA, Islam T, Islam S and Haque M. 2020. Microbial resistance movements: An overview of global public health threats posed by antimicrobial resistance, and how best to counter. Front Public Health 8: 535668. DOI: 10.3389/fpubh.2020.535668.
Disassa N, Sibhat B, Mengistu S, Muktar Y, Belina D. 2017. Prevalence and Antimicrobial Susceptibility Pattern of Escherichia coli O157:H7 Isolated from Traditionally Marketed Raw Cow Milk in and around Asosa Town, Western Ethiopia. Vet Med Int 2017: 9. DOI: 10.1155/2017/7581531.
Economou V, Gousia P. 2015. Agriculture and food animals are sources of antimicrobial-resistant bacteria. Infect Drug Resist 8: 49-61. DOI: 10.2147/IDR.S55778.
Effendi MH, Harijani N, Yanestria SM, Hastutiek P. 2018. Identification of shiga toxin-producing Escherichia coli in raw milk samples from dairy cows in Surabaya, Indonesia. Philippine J Ved Med 55 (SI): 109-114.
Effendi MH, Tyasningsih W, Yurianti YA, Rahmahani J, Harijani N, Plumeriastuti H. 2021. Presence of multidrug resistance (MDR) and extended beta-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swabs of broilers in several wet markets in Surabaya, Indonesia. Biodiversitas 22 (1): 304-310. DOI: 10.13057/biodiv/d220137 .
FAO, VMD. 2022. Tackling antimicrobial use and resistance in food-producing animals-Lessons learned in the United Kingdom. Rome. DOI: 10.4060/cc0927en.
Friese A, Schulz J, Laube H, von Salviati C, Hartung J, Roesler U. 2013. Faecal occurrence and emissions of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) and ESbl/AmpC-producing E. coli from animal farms in Germany. Berl Munch Tierarztl Wochenschr 126 (3-4): 175-180.
Ghai RR, Wallace RM, Kile JC, Shoemaker TR, Vieira AR, Negron ME, Shadomy SV, Sinclair JR, Goryoka GW, Salyer SJ, Behravesh CB. 2022. A generalizable one health framework for the control of zoonotic diseases. Sci Rep 12 (1): 8588. DOI: 10.1038/s41598-022-12619-1.
Giufre M, Mazzolini E, Cerquetti M, Brusaferro S, CCM2015 One-Health ESBL-producing Escherichia coli Study Group. 2021. Extended-spectrum ?-lactamase-producing Escherichia coli from extraintestinal infections in humans and from food-producing animals in Italy: a 'One Health' study. Int J Antimicrob Agents 58 (5): 106433. DOI: 10.1016/j.ijantimicag.2021.106433.
Guo J, Jiang B, Sumita, Wu C, Zhang Y, Li C. 2022. Review of the Distribution and Influence of Antibiotic Resistance Genes in Ballast Water. Water 14: 3501. DOI: 10.3390/w14213501.
Harijani N, Oetama SJT, Soepranianondo K, Effendi MH, Tyasningsih W. 2020. Biological hazard on multidrug resistance (MDR) of Escherichia coli collected from cloacal swab of broiler chicken on wet markets Surabaya. Indian J Forensic Med Toxicol 14 (4): 3239–3244. DOI: 10.37506/ijfmt.v14i4.12125.
Hooban B, Joyce A, Fitzhenry K, Chique C, Morris D. 2020. The role of the natural aquatic environment in the dissemination of extended spectrum beta-lactamase and carbapenemase encoding genes: A scoping review. Water Res 180: 115880. DOI: 10.1016/j.watres.2020.115880.
Huijbers PMC, Larsson DGJ, Flach CF. 2020. Surveillance of antibiotic resistant Escherichia coli in human populations through urban wastewater in ten European countries. Environ Pollut 261: 114200. DOI: 10.1016/j.envpol.2020.114200.
Hutinel M, Huijbers PMC, Fick J, Åhrén C, Larsson DGJ, Flach CF. 2019. Population-level surveillance of antibiotic resistance in Escherichia coli through sewage analysis. Euro Surveill 24 (37): 1800497. DOI: 10.2807/1560-7917.ES.2019.24.37.1800497.
Iwu CD, Korsten L, Okoh AI. 2020. The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health. Microbiologyopen. 9(9): e1035. DOI: 10.1002/mbo3.1035.
Ilbeigi K, Askari Badouei M, Vaezi H, Zaheri H, Aghasharif S, Kafshdouzan K. 2021. Molecular survey of mcr-1 and mcr-2 plasmid mediated colistin resistance genes in Escherichia coli isolates of animal origin in Iran. BMC Res Notes 14 (1): 107. DOI: 10.1186/s13104021055196.
Jaakkonen A, Castro H, Hallanvuo S, Ranta J, Rossi M, Isidro J, Lindstrom M, Hakkinen M. 2019. Longitudinal Study of Shiga Toxin-Producing Escherichia coli and Campylobacter jejuni on Finnish Dairy Farms and in Raw Milk. Appl Environ Microbiol. 85(7): e02910-18. DOI: 10.1128/AEM.02910-18.
Jang J, Hur H-G, Sadowsky MJ, Byappanahalli MN, Yan T, Ishii S. 2017. Environmental Escherichia coli: ecology and public health implications—a review. J Appl Microbiol. 123(3): 570-581. DOI: 10.111/jam.13468.
Khairullah AR, Sudjarwo SA, Effendi MH, Ramandininto SC, Gelolodo MA, Widodo A, Riwu KHP, Kurniawati DA, Rehman S. 2022. Profile of Multidrug Resistance and Methicillin-Resistant Staphylococcus aureus (MRSA) on dairy cows and risk factors from farmer. Biodiversitas 23 (6): 2853-2858. DOI: 10.13057/biodiv/d230610.
Kunhikannan S, Thomas CJ, Franks AE, Mahadevaiah S, Kumar S, Petrovski S. 2021. Environmental hotspots for antibiotic resistance genes. MicrobiologyOpen.10: e1197. DOI: 10.10002/mbo3.1197
Larsson DGJ, Flach CF. 2022. Antibiotic resistance in the environment. Nat Rev Microbiol. 20(5): 257-269. DOI: 10.1038/s41579-021-00649-x.
Machado EC, Freitas DL, Leal CD, de Oliveira AT, Zerbini A, Chernicharo CA, de Araújo JC. 2023. Antibiotic resistance profile of wastewater treatment plants in Brazil reveals different patterns of resistance and multi resistant bacteria in final effluents. Sci Total Environ. 857(Pt 1):159376. DOI: 10.1016/j.scitotenv.2022.159376.
Mariana C, Simner PJ, Bradford PA. 2021. Extended-spectrum ?-lactamases: an update on their characteristics, epidemiology and detection, JAC-Antimicrobial Resistance. 3(3). DOI: 10.1093/jacamr/dlab092.
Maulana KY, Pichpol D, Farhani NR, Widiasih DA, Unger F, Punyapornwithaya F, Meeyam T. 2021. Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia. Vet Integr Sci 19 (3): 525-535. DOI: 10.12982/VIS.2021.041.
Mills MC, Lee J. 2019. The threat of carbapenem-resistant bacteria in the environment: Evidence of widespread contamination of reservoirs at a global scale. Environ Pollut 255 (Pt 1): 113143. DOI: 10.1016/j.envpol.2019.113143.
Murugaiyan J, Kumar PA, Rao GS, Iskandar K, Hawser S, Hays JP, Mohsen Y, Adukkadukkam S, Awuah WA, Jose RAM, Sylvia N, Nansubuga EP, Tilocca B, Roncada P, Roson-Calero N, Moreno-Morales J, Amin R, Kumar BK, Kumar A, Toufik AR, Zaw TN, Akinwotu OO, Satyaseela MP, van Dongen MBM. 2022. Progress in Alternative Strategies to Combat Antimicrobial Resistance: Focus on Antibiotics. Antibiotics (Basel) 11 (2): 200. DOI: 10.3390/antibiotics11020200.
Ngene AC, Ohaegbu CG, Awom IE, Egbere JO, Onyimba IA, Coulthard OD, Umar U, Ohaeri1 CU, Nnadi NN, Aguiyi JC. 2021. High prevalence of multidrug resistant enterobacteriaceae isolated from wastewater and soil in Jos Metropolis, Plateau State, Nigeria.J. Bacteriol.Res. 13(2): 22-29. DOI: 10.5897/JBR2021.0336.
Niasono AB, Latif H, Purnawarman T. 2019. Antibiotic resistance to Escherichia coli bacteria isolated from broiler farms in Subang regency, West Java. J Vet 20 (2): 187-195. DOI: 10.19087/jveteriner.2019.20.2.187.
Partridge SR, Kwong SM, Firth N, Jensen SO. 2018. Mobile genetic elements associated with antimicrobial resistance. Clin Microbiol Rev 31 (4): e00088-17. DOI: 10.1128/CMR.00088-17.
Pazda M, Rybicka M, Stolte S, Piotr Bielawski K, Stepnowski P, Kumirska J, Wolecki D, Mulkiewicz E. 2020. Identification of selected antibiotic resistance genes in two different wastewater treatment plant systems in Poland: A Preliminary Study. Molecules 25 (12): 2851. DOI: 10.3390/molecules25122851.
Riwu KHP, Rantam FA, Effendi MH, Khairullah AR, Widodo A, Kurniawan SC, Ugbo EN. 2022. Molecular detection of extended spectrum ?-lactamase producing Klebsiella pneumoniaefrom wild deer. Biodiversitas 23 (8): 4256-4262. DOI: 10.13057/biodiv/d230849.
Sleeman JM, DeLiberto T, Nguyen N. 2017. Optimization of human, animal, and environmental health by using the One Health approach. J Vet Sci 18 (S1): 263-268. DOI: 10.4142/jvs.2017.18.S1.263.
Sun D, Jeannot K, Xiao Y and Knapp CW. 2019. Editorial: Horizontal gene transfer mediated bacterial antibiotic resistance. Front Microbiol 10: 1933. DOI: 10.3389/fmicb.2019.01933.
Suzuki Y, Hiroki H, Xie H, Nishiyama M, Sakamoto SH, Uemura R, Nukazawa K, Ogura Y, Watanabe T, Kobayashi I. 2022. Antibiotic-resistant Escherichia coli isolated from dairy cows and their surrounding environment on a livestock farm practicing prudent antimicrobial use. Int J Hyg Environ Health 240: 113930. DOI: 10.1016/j.ijheh.2022.113930.
Tyasningsih W, Ramandinianto SC, Ansharieta R, Witaningrum AM, Permatasari DA, Wardhana DK, Effendi MH, Ugbo EN. 2022. Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia. Veterinary World 15 (8): 2021-2028. DOI: 10.14202/vetworld.2022.2021-2028.
Wardhana DK, Effendi MH, Harijani N, Oei HK. 2020. Detection of extended-spectrum-beta-lactamase (ESBL) producing Escherichia coli in meat chicken from traditional market in Surabaya, East Java, Indonesia. Indian J Public Health Res Dev 11 (1): 1353-1357. DOI: 10.37506/v11/il/2020/ijphrd/194032.
Wardhana DK, Effendi MH, Safitri DA, Annisa S, Harijani N, Estoepangestie ATS, Maghfiroh L. 2021. Prevalence of Extended-Spectrum Beta-Lactamases in producing Escherichia coli in beef sold in traditional markets in Surabaya, Indonesia. Biodiversitas 22 (5): 2789-2793. DOI: 10.13057/biodiv/d220542.
Widodo A, Lamid M, Effendi MH, Khailrullah AR, Kurniawan SC, Silaen OSM, Riwu KHP, Yustinasari LR, Afnani DA, Dameanti FNAEP, Ramandinianto SC. 2023. Antimicrobial resistance characteristics of multidrug resistance and extended spectrum beta-lactamase producing Escherichia coli from several dairy farms in Probolinggo, Indonesia. Biodiversitas 24 (1): 215-221. DOI: 10.13057/biodiv/d240126.
Widodo A, Lamid M, Effendi MH, Khailrullah AR, Riwu KHP, Yustinasari LR, Kurniawan SC, Ansori ANM, Silaen OSM, Dameanti FNAEP. 2022. Antibiotic sensitivity profile of multidrug-resistant (MDR) Escherichia coli isolated from dairy cow's milk in Probolinggo, Indonesia. Biodiversitas 23 (10): 4971-4976. DOI: 10.13057/biodiv/d231002.
Widodo A, Effendi MH, Khairullah AR. 2020. Extended-spectrum beta-lactamase (ESBL)-producing Eschericia coli from livestock. Sys Rev Pharm 11 (7): 382-392. DOI: 10.31838/srp.2020.7.57.
Witaningrum AM, Wibisono FJ, Permatasari DA, Tyasningsih, Effendi MH, Kurniawan. 2020. Potential hazards of antibiotic resistance of Escherchia coli isolated from cloacal swab in several layer poultry farms, Blitar, Indonesia. Indian J Public Health Res Dev 11 (3): 2378-2384. DOI: 10.37506/ijphrd.v11i3.2764.
Yanestria SM, Dameanti FNAEP, Musayannah BG, Pratama JWA, Witaningrum AM, Effendi MH, Ugbo EN. 2022. Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia. Biodiversitas 23 (9): 4460-4465. DOI: 10.13057/biodiv/d230911.
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