Potential of dadiah kapau from Agam District, West Sumatra, Indonesia as a source of probiotics for health
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Abstract
Abstract. Roza E, Aritonang SN, Yellita Y, Susanty H, Rizqan, Pratama YE. 2021. Potential of dadiah kapau from Agam District, Indonesia as a source of probiotics for health. Biodiversitas 23: 564-571. Dadiah is a fermented product of buffalo milk prepared in a bamboo container as a traditional food in the Minangkabau tribe of West Sumatra, Indonesia. This study aimed to identify lactic acid bacteria that are potentially probiotics from dadiah products. The method used in this research includes an experimental survey with descriptive data analysis. The dadiah used in this study was taken from dadiah breeders and processors in Nagari Kapau, Agam District, West Sumatra, Indonesia. Tests for selection of probiotic candidates included macroscopic and microscopic observations, catalase test, fermentative type, resistance to gastric acid (pH 3), resistance to bile salts, antimicrobial activity using test bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes and Salmonella sp. Molecular identification was carried out by using 16S rRNA to determine the relationship of species using MEGA version 7.0 application. The study results revealed that the total colonies of dadiah kapau lactic acid bacteria were 128 x 108 CFU/g, and 12 isolates were obtained from the isolation results. Catalase negative for 12 isolates and 9 homofermentative types and 3 including heterofermentative types. Tests for resistance to gastric acid (pH 3) and bile salt resistance, the best viability was 84.61% and 86.51% respectively. The best antimicrobial activity showed a clear zone against the test bacteria E. coli O157, S. aureus, L. monocytogenes, and Salmonella sp. The phylogenetic analysis of the sequencing results shows that the dadiah kapau lactic acid bacteria are closely related to Lactobacillus plantarum.
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References
Afriani. 2010. Pengaruh penggunaan starter bakteri asam laktat Lacobacillus plantarum dan Lactobacillus fermentum terhadap total bakteri asam laktat, kadar asam dan nilai pH dadih susu sapi. Jurnal Ilmu-Ilmu Peternakan VIII(6):279-285.
Alonso S, Castro MC, Berdasco M, de la Banda IG, Moreno-Ventas X and de Rojas AH. 2018. Isolation and partial characterization of lactic acid bacteria from the gut microbiota of marine fishes for potential application as probiotics in aquaculture. Probiotics Antimicrob. Proteins, 11(2): 569-579.
Amezquita A and Brashears MM. 2002. Competitive inhibition of Listeria monocytogenes in ready-to-eat meat products by lactic acid bacteria. Journal of Food Protect, 65: 316-325.
Azat R, Liu Y, Li W and Kayir A. 2016. Probiotic properties of lactic acid bacteria isolated from traditionally fermented Xinjiang cheese. Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology). 17(8):597-609.
Bustos AY, Valdez GF, Fadda S and Taranto MP. 2018. New insights into bacterial bile resistance mech anisms: The role of bile salt hydrolase and its impact on human health. Food Res. Int., 112: 250-262.
Carr FJ, Chill D and Maida N. 2002. The lactic acid bacteria: a literature survey. Critical Reviews in Microbiology, 8:281-370.
Dallal SMM, Davoodabadi A, Abdi M, Hajiabdolbaghi M, Sharifi YMK, Douraghi M and Tabatabaei SMB. 2017. Inhibitory effect of Lactobacillus plantarum and Lb. fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. New Microbes and New Infections, 15, 9–13.
Desniar I, Rusmana, Suwanto A and Mubarik NR. 2013. Characterization of lactic acid bacteria isolated from an Indonesian fermented fish (bekasam) and their antimicrobial activity against pathogenic bacteria.
Emir J Food Agric. 25 (6): 489-494.
Evivie SM, Huo GC, Igene OJ and Bian X. 2017. Some current applications, limitations and future perspec tives of lactic acid bacteria as probiotics. Food Nutr. Res., 61(1): 1318034.
Food and Agriculture Organization of United Nations & Organization (FAO). 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. London, London Ontario.
Guan X, Xu Q, Zheng Y, Qian L and Lin B. 2017. Screening and characterization of lactic acid bacterial strains that produce fermented milk and reduce cholesterol levels. Braz. J. Microbiol, 48(4): 730-739.
Harnentis H, Nurmiati N, Marlida Y, Adzitey F dan Huda N. 2019. ?-Aminobutyric acid production by selected lactic acid bacteria isolate of an Indonesian indigenous fermented buffalo milk (dadih) origin. Veterinary World, 12(8): 1352-1357.
Harnentis H, Marlida Y, Nur YS, Wizna W, Santi MA, Septiani N, Adzitey F and Huda N. 2020. Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia, Veterinary World, 13(9): 1922-1927.
Harun H, Wirasti Y, Purwanto B and Purwati E. 2020. Characterization of Lactic Acid Bacteria and Determination of Antimicrobial Activity in Dadih from Air Dingin Alahan Panjang District, Solok Regency-West Sumatera. Sys Rev Pharm. 11(3): 583 586.
Jain N, Mehta A and Bhart V. 2017. Screening, characterization, and in vitro evaluation of probiotic of Lactobacillus strains. Asian Journal of Pharmaceutical and Clinical Research. 10(8): 288-93.
Juliyarsi I, Hartini H, Yuherman, Djamaan A, Arief, Purwanto H, Aritonang SN, Hellyward J and Purwati E. 2018. Characterization of Lactic Acid Bacteria and Determination of Antimicrobial Activity in Tempoyak from Padang Pariaman District, West Sumatra, Indonesia. Pakistan Journal of Nutrition. 17 (10): 506-511.
Korhonen. J. 2010. Forestry and Naturals Sciences: Antibiotic Resistance of Lactid acid Bacteria, University of Eastern Finland.
Kwarteng JO, Debrah KT, Akabanda F and Jesperen L. 2015. Technological properties and probiotic potential of Lactobacillus fermentum strains isolated from West African fermented millet dough. BMC Microbiol., 15: 261.
Lebeer S, Bron PA, Marco ML, van Pijkeren JP, Motherway MO, Hill C and Klaenhammer T. 2018. Identification of probiotic effector molecules: Present state and future perspectives. Curr. Opin. Biotechnol., 49: 217-223.
Liu Y, Tang H, Lin Z and Xu P. 2015. Mechanisms of acid tolerance in bacteria and prospects in biotechnology and bioremediation. Biotechnol Adv. 33(7):1484–1492.
Melia S, Ferawati, Yuherman, Jaswandi, Purwanto H and Purwati E. 2018. Probiotic Characterization Of Lactic Acid Bacteria Isolated From Raw Milk (Buffallo, Cow, And Goat) From West Sumatera, Indonesia. Asian Journal Of Microbiology, Biotechnology And Environmental Sciences. Vol 20 : S131-S139.
Melia S, Purwati E, Yuherman, Jaswandi, Aritonang SN and Silaen M. 2017. Characterization of the Antimicrobial Activity of Lactic Acid Bacteria Isolated from Buffalo Milk in West Sumatera (Indonesia) Against Listeria monocytogenes. Pakistan Journal of Nutrition, 16 (8): 645-650.
Melia S, Purwati E, Kurnia YF and Pratama DR. 2019. Antimicrobial potential of Pediococcus acidilactici from Bekasam, fermentation of sepat rawa fish (Tricopodus trichopterus) from Banyuasin, South Sumatra, Indonesia. Biodiversitas J. 3532-3538.
Mojgani N, Hussaini F and Vaseji N. 2015. Characterization of indigenous Lactobacillus strains for probiotic properties. Jundishapur J. Microbiol, Vol. 8.
Morales G, Sierra P, Mancilla, Paredes A, Loyola LA, Gallardo O and Borquez J. 2003. Secondary metabolites from four medicinal plants from Northern Chile, antimicrobial activity, and biotoxicity against artemia salina. J. Chile Chem 48(2).
Mulaw G, Tessema TS, Muleta D and Tesfaye A. 2019. In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products. Int. J. Microbiol, 7179514.
Nuraida. L. 2015. A review: Health promoting lactic acid bacteria in traditional Indonesian fermented foods. Sci. Hum. Wellness, 4(2): 47-55.
Nurnaafi A, Setyaningsih I and Desniar I. 2015. Potensi probiotik bakteri asam laktat asal bekasam ikan nila. J. Teknol. Ind. Pangan, 26(1): 109-114.
Obdak A, ZieliNska D, Rzepkowska A and Krajewska DK. 2017. Comparison of antibacterial activity of Lactobacillus plantarum strains isolated from two different kinds of regional cheeses from Poland: Oscypek and korycinski cheese. Biomed Res. Int.
Palachum W, Chisti Y and Choorit W. 2018. In vitro assessment of probiotic potential of Lactobacillus plantarum WU-P19 isolated from a traditional fermented herb. Ann. Microbiol, 68(5): 79-91.
Pan X, Chen F, Wu T, Tang H and Zhao Z. 2009. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control, 20: 598-602.
Pato U. 2003. The potential of lactic acid bacteria isolated from dadiah to reduce the risk of cancer. J. Nat. Indonesia,5?(2): 162-166.
Public Health England. 2014. Identification of Staphylococcus Species, Micrococcus Species and Rothia Species. UK Standards for Microbiology Investigations, UK.
Putri, WDR, Haryadi, DW. Marseno dan Cahyanto MN. 2012. Isolasi dan karakteristik bakteri asam laktat amilolitik selama fermentasi Growol, makanan tradisional Indonesia. Jurnal Teknologi Pertanian, 13(1):52-60.
Purwati E, Syukur S dan Hidayat Z. 2005. Lactobacilus sp. Isolasi dari Biovicophitomega sebagai Probiotik. Proceeding Lembaga Ilmu Pengetahuan Indonesia, Jakarta.
Ramirez-Chavarin ML, C.Wacher, C.A. Eslava-Campos and M.L. Prez-Chabela. 2013. Probiotic potential of thermotolerant lactic acid bacteria strains isolated from cooked meat products. International. Food research Journal, 20(2): 991- 1000.
Rashid S and M. Hassanshahian. 2014. Screening, Isolation and Identification of Lactic Acid Bacteria From a Traditional Dairy Product of Sabzevar, Iran. Int J Enteric Pathog. 2: e18393.
Reuben RC, Coy PC, Sarlkar SR, Alam ASM and Jahid IK. 2019. Characterization and evaluation of lactic acid bacteria from indigenous raw milk for potential probiotic properties. J. Dairy Sci., 103(2): 1223-1237.
Salminen S, Wright AV dan Ouwehand A. 2004. Lactic acid bacteria: Microbiology and Functional Aspects. Marcel Dekker. Inc. New York . 3 thedition.
Shafakatullah N and M Chandra. 2014. Screening of raw buffalo’s milk from Karnataka for potential probiotic strains. Research Journal of Recent Sciences. Vol. 3(9), 73-78.
Setiyanto H, Miskiyah, Abubakar, Usmiati S, Broto W, Sukasih dan Edial A. 2009. Perbaikan proses dan pengemasan dadih sebagai probiotik dengan daya simpan sampai 20 hari. Laporan penelitian. Bogor (Indonesia): Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian.
Somashekaraiah R, Deepthi BV and Sreenivasa MY. 2019. Probiotic properties of lactic acid bacteria isolated from neera: A naturally fermenting coconut palm nectar. Front. Microbiol., 10: 1382.
Surono IS. 2003. In vitro probiotic properties of indigenous dadih lactic acid bacteria. Asian-Australasian J Anim Sci. 16:726-731.
Surono IS. 2004. Probiotics for Fermented Milk and Health, the Indonesian Food and Beverage Entrepreneurs Foundation (YAPMMI). TRICK Jakarta, Indonesia.
Sunarlim R. 2009. Potensi Lactobacillus sp asal dari dadih sebagai starter pada pembuatan susu fermentasi khas Indonesia. Bulletin Teknologi Pasapanen Pertanian, volume 5.
Sunaryanto R and Marwoto B. 2013. Isolation, identification, and characterization of lactic acid bacteria from buffalo milk dadiah. J. Sains Teknologi Indonesia, 14?(3): 228-233.
Suryani Y, Astuti B, Oktavia dan Umniyati S. 2010. Isolasi dan karakterisasi bakteri asam laktat dari limbah kotoran ayam sebagai agensi probiotik dan enzim kolesterol reduktase. Prosiding Seminar Nasional Biologi, 138-147.
Syukur S dan Purwati E. 2013. Bioteknologi Probiotik untuk Kesehatan Masyarakat. Penerbit Andi, Yogyakarta.
Syukur S, Fachrial E and Jamsari. 2014. Isolation, antimicrobial activity and protein bacteriocin characterization of lactic acid bacteria isolated from dadih in Solok, West Sumateran Indonesia. Reseach Journal of Pharmaceutical, Biological and Chemical Science. P. 1096-1104.
Syukur S, Utami LS, Purwati E, Urnemi dan Jamsari. 2011. Screening and invitro antimicrobial, protease activities from West Sumatera, Indonesia, Proseding Seminar Internasional HKI, Pekanbaru.
Tokatli M, G. Gulgor, S.B. Elmaci, N.A. Isleyen and F. Ozcelik. 2015. In vitro properties of potential probiotic Indigenous lactic acid bacteria originating from traditional pickles. Biomed. Res. Int., 2015: 315819.
Ullah N, Wang X, Wu J, Guo Y, Ge H, Li T, Khan S, Li Z and Fengm X. 2017. Purification and pri mary characterization of a novel bacteriocin, LiN333, from Lactobacillus casei, an isolate from a Chinese fermented food. Food Sci. Technol, 84: 867-875.
Usmiati S, Broto W and Setiyanto H. 2011. Characteristics of cow milk dadiah using a starter of probiotic bacteria. J. Ilmu Ternak Vet., 16?(2): 141-153.
Unus. 2005. Basic Microbiology. Publisher Papas Sinar Sinanti.
Vasiee AR, Y.F. Tabatabaei, A. Mortazavi and M.R. Edalatian. 2014. Isolation, identification and characterization of probiotic Lactobacilli spp. From Tarkhineh. International Food Research Journal. 21 : 2487-2492.
Willey JM, Sherwood LM and Woolverton CJ. 2008. Presscout, Harley and Klein’s Microbiology. 7th Edn, Mc Graw Hill Companies, New York, USA.
Yang, Ziheng and Rannala B. 2012. Molecular Phylogenetics: Principles and Practice. Nature Reviews Genetics 13:303-314.
Zhang B, Wang Y, Tan Z, Li Z and Jiao Z. 2016. Screening of Probiotic Activities of Lactobacilli Strains Isolated from Traditional Tibetan Qula, A Raw Yak Milk Cheese. Asian-Australas J Anim Sci. 29: 1490-1499.
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