Synchronization of GnRH and PGF2? on estrus response, pregnancy, progesterone hormones in crossing of Swamp Buffalo and Water Buffalo in West Sumatra, Indonesia
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Abstract. Roza E, Aritonang SN, Susanti H, Sandra A. 2019. Synchronization of GnRH and PGF2? on estrus response, pregnancy, progesterone hormones in crossing of swamp buffalo and water buffalo in West Sumatra, Indonesia. Biodiversitas 20: 2910-2914. This study aims to determine the effect of GnRH and PGF2? synchronization on estrus emergence, pregnancy percentage, progesterone hormone levels and blood profile from artificial insemination (AI) of swamp and water buffalo crossing in Sijunjung, West Sumatra. The samples were 21 female swamp buffaloes with criteria clinically healthy, age ? 2.5 years and not pregnant. All buffalos on the first day were synchronized using 250 ?gGnRH (Fertagyl®, Intervet International). All of the buffaloes received 12.5 ?g PGF2? on the seventh day after GnRH injection. On the second day after injection of PG2?, the observation of estrus was carried out, the buffalos with estrus symptoms appeared after performing AI for 18 hours which the estrus symptoms was seen using a 0.5 ml Murrah buffalo semen with a sperm concentration of 500 million. Blood serum of 3-5 ml for the examination of progesterone levels was taken on days 21, 24, and 27 after appeared in AI. Hormone analysis was performed using the Enzyme-Linked Immunoabsorbent Assay (ELISA) method. The variables measured were the percentage of estrus, pregnancy, progesterone hormone levels, and blood profile. Pregnancy examination (PE) was carried out after 90 days in AI through rectal palpation. The data were analyzed descriptively. The results showed synchronization of GnRH and PG2? hormones in buffalo cattle which had 100% estrus, 66.67% pregnancy after AI, pregnant buffalo progesterone concentration 5.09-8.87 ng/mL and non-pregnant 1.11-3.45 ng/mL, total blood protein 7.9 g/L and blood glucose 86.86 mg/dL. The conclusion of this study is that the combination of GnRH and PG2? gives a clear appearance of estrus, progesterone hormone levels, and optimal buffalo blood profile.
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References
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Brito, LFC, R Satrapa, EP Marson and JP Kastelic. 2002. Efficacy of PGF2? to synchronize estrus in water buffalo cows (Bubalus bubalis) is dependent upon plasma progesterone concentration, corpus luteum size and ovarian follicular status before treatment. Anim. Reprod. Sci. 73: 23-35.Prentice Hall Inc.
Chandrahar, D, RP Tiwari, MK. Awasthi and GK Dutta. 2003. Serum biochemical profile of repeat breeder cows. Indian J. Anim Reprod. 24:125-127.
De Rensis, F and F Lopez-Gatius. 2007. Protocols for synchronizing estrus and ovulation in buffalo (Bubalus bubalis): A review. Theriogenology 67: 209-216.
Ditjen Peternakan Dan Kesehatan Hewan. 2015. Statistik peternakan 2015. Direktorat Jenderal Peternakan. Departemen Peternatian.
Fahlevi1, Z, Al Azhar, A. Sayuti, M Isa, M Adam and Herrialfian. 2017. Blood Chemistry of Local Swamp Buffaloes (Bubalus bubalis) of Aceh. Jurnal Medika Veterinaria. 11 (1): 66-70.
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Ginther, OJ, HK Shrestha, MJ Fuenzalida, AK Shahiduzzaman, MA Hannan and MA Beg. 2010. Intrapulse temporality between pulses of a metabolite of prostaglandin F2á and circulating concentrations of progesterone before, during, and after spontaneous luteolysis in heifers. Theriogenology 74: 1179-1186.
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Mulligan, FJ, L O’Grady, DA. Rice, and ML Doherty. 2006. Nutrition and fertility in dairy cows. Irish Vet. J. 60:15-20.
Muhamad, F, A Sawar, CS Hayat and MI Anwar. 2000. Peripheral Plasma Progesterone Concentration During Early Pregnancy in Holstein Friesian Cows. Pakistan Vet J 20 (4) : 166-168
Murray, RK, DK. Granner, PA Mayes dan VW Rodwell. 2003. Biokimia Harper. Edisi ke-25. Penerbit Buku Kedokteran EGC, Jakarta.
Neglia, G, B Gasparrini, RD Palo, CD Rosa, L Zicarelli and G Campanile. 2003. Comparison of pregnany rates with two oestrus synchronization protocols in Italian Mediterranean buffalo cows. Theriogenology 60: 125-33.
Paul, V and B Prakash. 2005. Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah Buffaloes (Bubalus bubalis). Theriogenal. 64: 1049-1060.
Pradhan, R and N Nakagoshi. 2008. Reproductive Disorders in Cattle doe to Nutritional Status. J of Inter Dev and Coop 14: 45-66
Rajamahendran, R, JD Ambrose, M Aali, N Rramakrishnappa, N Giritharan and J Small. 2002. Hormonal Treatment Following Breeding to Increase Pregnancy Rates IN Cattle. J. Biotech. Anim. Reprod. 9: 151-160.
Roza, E, SN Aritonang dan A Sandra. 2015. The Haematology of Lactating Buffalo Fed Local Foilage as Feed Supplement. Journal of Agricultural Science and Technology A. Vol.5, No. 10 pp:839-445.
Roza, E, SN Aritonang dan Lendrawati. 2017. Kajian Gambaran Darah Dan Karakteristik Susu Kerbau Sungai Dan Kerbau Rawa Dalam Pencapaian Swasembada Susu 2020. Laporan Penelitian BOPTN. Unand.
Ryu, YH, JY Yang, DS Seo and Y Ko. 2003. Effect of serum IGF-I on progesterone concentration during early pregnancy in Korean Native Cattle (Hanwoo). Asian-Aust.J Anim Sci. 16:2 ; 176-179.
Senger, PL. 2005. Reproductive cyclicity – the follicular phase. In: Pathways to Pregnancy and Parturition. 2nd Revised Edition. Washington State University Research & Technlogy Park. Current Conceptions, Inc. p.164- 187.
Siregar, TN. 2008. Upaya Meningkatkan Intensitas Berahi Pada Kerbau Dalam Hubungannya Dengan Peningkatan Angka Konsepsi Hasil Inseminasi Buatan. Jurnal Ilmiah Ilmu-Ilmu Peternakan. Vol. XI, No.4, Hlm 69-74.
Steel, RGD and JH Torrie. 1991. Principles and Procedures of Statistics. McGraw-Hill Book Co. Inc. New York.
Yendraliza, BP Zespin, Z Udin, dan Jaswandi. 2012. Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF2?. JITV 17(2): 107-111
Arthur, GH., EN. David and H Pearson. 2001. Veterinary reproduction and obstetrics. 8th edition. Bailiere Tindall. London. United Kingdom.
Bartolome, JA., FT Silvestre, ACM Artechte, S Kamimura, LF Archbald and WW Thatcher. 2002. The Use of Ovsynch and Heatsynch for Resynchronization of Cows Open at Pregnancy Diagnosis by Ultrasonography. J. Dairy Sci. 81: 390-342.
Bearden, JH, WF John and TW Scott. 2004. Applied animal reproduction 6th. New Jersey: Pearson
Berber, RCA, EH. Madureira and PS Barusselli. 2002. Comparison of two ovsynch protocols (GnRH vs. LH) for fixed timed insemination in buffalo (Bubalus bubalis).
Belstra, BA. 2003. Parity Associated Changes In Reproductive Performance: Phisiological Basis and Record Keeping Artefact. http:www.mark.asci..ncsu.edu/swine report/2003/belstra.htm.
Brito, LFC, R Satrapa, EP Marson and JP Kastelic. 2002. Efficacy of PGF2? to synchronize estrus in water buffalo cows (Bubalus bubalis) is dependent upon plasma progesterone concentration, corpus luteum size and ovarian follicular status before treatment. Anim. Reprod. Sci. 73: 23-35.Prentice Hall Inc.
Chandrahar, D, RP Tiwari, MK. Awasthi and GK Dutta. 2003. Serum biochemical profile of repeat breeder cows. Indian J. Anim Reprod. 24:125-127.
De Rensis, F and F Lopez-Gatius. 2007. Protocols for synchronizing estrus and ovulation in buffalo (Bubalus bubalis): A review. Theriogenology 67: 209-216.
Ditjen Peternakan Dan Kesehatan Hewan. 2015. Statistik peternakan 2015. Direktorat Jenderal Peternakan. Departemen Peternatian.
Fahlevi1, Z, Al Azhar, A. Sayuti, M Isa, M Adam and Herrialfian. 2017. Blood Chemistry of Local Swamp Buffaloes (Bubalus bubalis) of Aceh. Jurnal Medika Veterinaria. 11 (1): 66-70.
FAOSTAT. 2012. Statisticaldatabase.http://faostat.fao.org/. [12September2012].Gohl, B., 1981. Tropical Feeds. Feed Information Summaries and Nutritive Value. FAO-UN, Bangkok.
Frandson, RD. 1996. Anatomi dan Fisiologi Ternak. Gajah mada University Press. Yogyakarta
Fricke, PM and RD Shaver. 2007. Managing reproductive disorders in dairy cows. www.wisc.edudysciuwexrep.
Ginther, OJ, HK Shrestha, MJ Fuenzalida, AK Shahiduzzaman, MA Hannan and MA Beg. 2010. Intrapulse temporality between pulses of a metabolite of prostaglandin F2á and circulating concentrations of progesterone before, during, and after spontaneous luteolysis in heifers. Theriogenology 74: 1179-1186.
Hafez, ESE. 2000. Anatomy of Male Reproduction. “In Reproduction in Farm Animals”. Hafez ( 7 th ed.). Lippincott William & Wilkins. A Wolter Kluwer Company.
Hafez, ESE and B Hafez. 2000. Reproduction in Farm Animals 7th. Maryland: Lippincott William and Wilkins.
Herdis. 2011. Respon Estrus Domba Garut Betina Pada Perlakuan Laserpuntur Dengan Fase Reproduksi Yang Berbeda. Jurnal Sains dan Teknologi Indonesia Vol. 13, No. 3, Hlm.171-176.
Ibrahim, L. 2008. Produksi susu, reproduksi dan manajemen kerbau perah di Sumatera Barat. Jurnal Peternakan, 5 (1): 1- 9.
Irikura, CR, JCP. Ferreira, I Martin, LU Cimenes, E Oba and AM Jorge. 2003. Follicular dynamics in buffalo heifers (Bubalus bubalis) using the GnRHPGF2?- GnRH protocol. Buffalo J. 3: 323-327.
Jainudeen, MR and ESE Hafez. 2000. Reproductive Failure in Females. In Reproduction in Farm Animal. Hafez E.S.E (Ed.) 7th ed. Lea and Fabiger, Philadelphia
Khan, DJ, HA Garberick, CJ Bierschwal, RG Elmore and RS Youngqvist. 2009. Reproductive hormones associated with normal and abnormal changes in ovarian follicles in postpartum dairy buffalo. Journal of Dairy Science 62: 1290-1291.
Leitman, NR, DC Busch, DA Mallory, DJ Wilson, MR Ellersieck, MF Smith and DJ Patterson. 2009. Comparison of long-term CIDRbased protocol to synchronize estrus in beef heifers. Animal Reproduction Science 114: 345-355.
Lemcke, B. 2004. Production Specialized Quality Meat Products from Water Buffalo: Tenderbuff. The Carabao – Bubalus Bubalis Carabanensis. Proceedings 7th World Buffalo Conggress. 20-23 October, 2004: 49-54. Makati City, Philippines.
McDonald, LE. 2000. Veterinary Endocrinology and Reproduction. 3rd Ed. Bailliere Tindall, London. Pp 315-367
Metwelly, KK. 2001. Postpartum anestrus in buffalo cows; cause and treatment. Proc. sixth. Sci. Congr. Egypt. Soe. Cattle Disease. Assiut. University, Egypt. pp. 259-267.
Mihaiu, M, A Lapusan, C Bele, and R Mihaiu. 2011. Compositional Particularities of the Murrah Hybrid Buffalo Milk and its Suitability for Processing in the Traditional System of Romania. Bulletin UASVM, Veterinary Medicine 68(2):p. 216 – 221
Mulligan, FJ, L O’Grady, DA. Rice, and ML Doherty. 2006. Nutrition and fertility in dairy cows. Irish Vet. J. 60:15-20.
Muhamad, F, A Sawar, CS Hayat and MI Anwar. 2000. Peripheral Plasma Progesterone Concentration During Early Pregnancy in Holstein Friesian Cows. Pakistan Vet J 20 (4) : 166-168
Murray, RK, DK. Granner, PA Mayes dan VW Rodwell. 2003. Biokimia Harper. Edisi ke-25. Penerbit Buku Kedokteran EGC, Jakarta.
Neglia, G, B Gasparrini, RD Palo, CD Rosa, L Zicarelli and G Campanile. 2003. Comparison of pregnany rates with two oestrus synchronization protocols in Italian Mediterranean buffalo cows. Theriogenology 60: 125-33.
Paul, V and B Prakash. 2005. Efficacy of the Ovsynch protocol for synchronization of ovulation and fixed-time artificial insemination in Murrah Buffaloes (Bubalus bubalis). Theriogenal. 64: 1049-1060.
Pradhan, R and N Nakagoshi. 2008. Reproductive Disorders in Cattle doe to Nutritional Status. J of Inter Dev and Coop 14: 45-66
Rajamahendran, R, JD Ambrose, M Aali, N Rramakrishnappa, N Giritharan and J Small. 2002. Hormonal Treatment Following Breeding to Increase Pregnancy Rates IN Cattle. J. Biotech. Anim. Reprod. 9: 151-160.
Roza, E, SN Aritonang dan A Sandra. 2015. The Haematology of Lactating Buffalo Fed Local Foilage as Feed Supplement. Journal of Agricultural Science and Technology A. Vol.5, No. 10 pp:839-445.
Roza, E, SN Aritonang dan Lendrawati. 2017. Kajian Gambaran Darah Dan Karakteristik Susu Kerbau Sungai Dan Kerbau Rawa Dalam Pencapaian Swasembada Susu 2020. Laporan Penelitian BOPTN. Unand.
Ryu, YH, JY Yang, DS Seo and Y Ko. 2003. Effect of serum IGF-I on progesterone concentration during early pregnancy in Korean Native Cattle (Hanwoo). Asian-Aust.J Anim Sci. 16:2 ; 176-179.
Senger, PL. 2005. Reproductive cyclicity – the follicular phase. In: Pathways to Pregnancy and Parturition. 2nd Revised Edition. Washington State University Research & Technlogy Park. Current Conceptions, Inc. p.164- 187.
Siregar, TN. 2008. Upaya Meningkatkan Intensitas Berahi Pada Kerbau Dalam Hubungannya Dengan Peningkatan Angka Konsepsi Hasil Inseminasi Buatan. Jurnal Ilmiah Ilmu-Ilmu Peternakan. Vol. XI, No.4, Hlm 69-74.
Steel, RGD and JH Torrie. 1991. Principles and Procedures of Statistics. McGraw-Hill Book Co. Inc. New York.
Yendraliza, BP Zespin, Z Udin, dan Jaswandi. 2012. Post-partum reproductive appearance of buffalo at various levels of GnRH and synchronized with PGF2?. JITV 17(2): 107-111
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