Short Communication: Identification of culturable marine fungi and bacteria from coastal region in Brunei Darussalam
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Abstract. Taha H, Shivanand P, Zainudin MAA, Hadanan NA. 2021. Short Communication: Identification of culturable marine fungi and bacteria from coastal region in Brunei Darussalam. Biodiversitas 22: 1326-1331. Microbial diversity in Brunei Darussalam located on northwest coast of Borneo is not well explored. This study aimed to isolate and identify culturable marine fungi and bacteria from coastal regions. Microbial identification was carried out via DNA barcoding using rRNA-ITS marker for fungi and 16S rRNA marker for bacteria. Nine marine fungal isolates were identified to five genera as Aspergillus, Penicillium, Phialemoniopsis, Purpureocillium and Trametes. However, only one genus, Pseudoalteromonas was identified from ten marine bacterial isolates with at least three different Pseudoalteromonas species expected from the isolation. This study provided an insight into the diversity of culturable marine microbes from a coastal ecosystem. As the microbes were found culturable and can easily grow under saline conditions, they are potential of considerable biotechnological interest.
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References
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Yue Y, Yu H, Li R, Xing R, Liu S, Li P. 2015. Exploring the antibacterial and antifungal potential of jellyfish-associated marine fungi by cultivation-dependent approaches. PLoS ONE 10 (12): e0144394. DOI: 10.1371/journal.pone.0144394
Zakaria L, Yee TL, Zakaria M, Salleh B. 2011. Diversity of microfungi in sandy beach soil of Teluk Aling, Pulau Pinang. Tropical Life Sciences Research 22 (1): 71–80.
Zhang L, An R, Wang J, Sun N, Zhang S, Hu J, Kuai J. 2005. Exploring novel bioactive compounds from marine microbes. Current Opinion in Microbiology 8 (3): 276–281. DOI: 10.1016/j.mib.2005.04.008.
Amer MS, Ellatif HHA, Hassan SWM, Aboelela GM, Gad AM. 2019. Characterization of some fungal strains isolated from the Eastern coast of Alexandria, Egypt, and some applications of Penicillium crustosum. The Egyptian Journal of Aquatic Research 45 (3): 211–217. DOI: 10.1016/j.ejar.2019.06.006
Carroll AR, Copp BR, Davis RA, Keyzers RA, Prinsep MR. 2019. Marine natural products. Natural Product Reports 36: 122–173. DOI: 10.1039/c8np00092a
Cohen E, Koch L, Thu KM, Rahamim Y, Aluma Y, Ilan M, Yarden O, Carmeli S. 2011. Novel terpenoids of the fungus Aspergillus insuetus isolated from the Mediterranean sponge Psammocinia sp. collected along the coast of Israel. Bioorganic & Medicinal Chemistry 19 (22): 6587–6593. DOI: 10.1016/j.bmc.2011.05.045
Gonçalves MFM, Santos L, Silva BMV, Abreu AC, Vicente TFL, Esteves AC, Alves A. 2019. Biodiversity of Penicillium species from marine environments in Portugal and description of Penicillium lusitanum sp. nov., a novel species isolated from sea water. International Journal of Systematic and Evolutionary Microbiology 69 (10): 3014–3021. DOI: 10.1099/ijsem.0.003535
Danovaro R, Pusceddu A. 2007. Biodiversity and ecosystem functioning in coastal lagoons: does microbial diversity play any role? Estuarine, Coastal and Shelf Science 75 (1-2): 4–12. DOI: 10.1016/j.ecss.2007.02.030
Deshmukh SK, Prakash V, Ranjan N. 2018. Marine fungi: a source of potential anticancer compounds. Frontiers in Microbiology 8: 2536. DOI: 10.3389/fmicb.2017.02536
Holmström C, Kjelleberg S. 1999. Marine Pseudoalteromonas species are associated with higher organisms and produce biologically active extracellular agents. FEMS Microbiology Ecology 30 (4): 285–293. DOI: 10.1111/j.1574-6941.1999.tb00656.x
Justo A, Hibbett DS. 2011. Phylogenetic classification of Trametes (Basidiomycota, Polyporales) based on a five-marker dataset. Taxon 60 (6): 1567–1583. DOI: 10.1002/tax.606003
Kaur A, Rogers KD, Swenson DE, Dowd PF, Wicklow DT, Gloer JB. 2014. Bioactive natural products from fungicolous Hawaiian isolates: secondary metabolites from a Phialemoniopsis sp. Mycology 5 (3): 120–129. DOI: 10.1080/21501203.2014.931309
Khalmuratova I, Kim H, Nam YJ, Oh Y, Jeong MJ, Choi HR, You YH, Choo YS, Lee IJ, Shin JH, Yoon H, Kim JG. 2015. Diversity and plant growth promoting capacity of endophytic fungi associated with halophytic plants from the west coast of Korea. Mycobiology 43 (4): 373–383. DOI: 10.5941/MYCO.2015.43.4.373
Kneževi? A, Staji? M, Sofreni? I, Stanojkovi? T, Milovanovi? I, Teševi? V, Vukojevi? J. 2018. Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia. PloS one 13 (8): e0203064. DOI: 10.1371/journal.pone.0203064
Kumar S, Stecher G, Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33 (7): 1870–1874. DOI: 10.1093/molbev/msw054
Li L, Singh P, Liu Y, Pan S, Wang G. 2014. Diversity and biochemical features of culturable fungi from the coastal waters of Southern China. AMB Express 4: 60. DOI: 10.1186/s13568-014-0060-9
Mahanty S, Bakshi M, Ghosh S, Chatterjee S, Bhattacharyya S, Das P, Das S, Chaudhuri P. 2019. Green synthesis of iron oxide nanoparticles mediated by filamentous fungi isolated from Sundarban mangrove ecosystem, India. BioNanoScience 9: 637–651, DOI: 10.1007/s12668-019-00644-w
Manohar CS, Raghukumar C. 2013. Fungal diversity from various marine habitats deduced through culture-independent studies. FEMS Microbiology Letters 341 (2): 69–78. DOI: 10.1111/1574-6968.12087
Mathan S, Smith AA, Kumaran J, Prakash S. 2011. Anticancer and antimicrobial activity of Aspergillus protuberus SP1 isolated from marine sediments of South Indian coast. Chinese Journal of Natural Medicines 9 (4): 286–292. DOI: 10.1016/S1875-5364(11)60066-6
Migahed FF. 2003. Distribution of fungi in the sandy soil of Egyptian beaches. Mycobiology 31 (2): 61–67. DOI: 10.4489/MYCO.2003.31.2.061
Perdomo H, Garcia D, Gene J, Cano J, Sutton DA, Summerbell R, Guarro J. 2013. Phialemoniopsis, a new genus of Sordariomycetes, and new species of Phialemonium and Lecythophora. Mycologia 105 (2): 398–421. DOI: 10.3852/12-137
Qin QL, Li Y, Zhang YJ, Zhou ZM, Zhang WX, Chen XL, Zhang XY, Zhou BC, Wang L, Zhang YZ. 2011. Comparative genomics reveals a deep-sea sediment-adapted life style of Pseudoalteromonas sp. SM9913. The ISME journal 5 (2): 274–284. DOI: 10.1038/ismej.2010.103
Richards GP, Needleman DS, Watson MA, Polson SW. 2019. Whole-genome sequences of two Pseudoalteromonas piscicida strains, DE1-A and DE2-A, with strong antibacterial activity against Vibrio vulnificus. Microbiology Resource Announcements 8 (1): e01451-18. DOI: 10.1128/MRA.01451-18
Rédou V, Navarri M, Meslet-Cladière L, Barbier G, Burgaud G. 2015. Species richness and adaptation of marine fungi from deep-subseafloor sediments. Applied and Environmental Microbiology 81 (10): 3571–3583. DOI: 10.1128/AEM.04064-14
Siegenthaler A, Wangensteen OS, Benvenuto C, Campos J, Mariani S. 2018. DNA metabarcoding unveils large-scale trophic variation in a widespread coastal opportunist. Molecular Ecology 28 (2): 232–249. DOI: 10.1111/mec.14886
Taha H, Shivanand P, Shaminan NIN, Osman M, Abdul-Halim AMAA, Abdullah M. 2020a. Isolation and identification of culturable bacteria and fungi from mixed dipterocarp and mangrove forests of Brunei Darussalam. Proceedings of the National Academy of Sciences India Section B Biological Sciences 90 (3): 523–530. DOI: 10.1007/s40011-019-01119-4
Taha H, Shivanand P, Khoo DH, Mohammad YH, Matussin NBA, Metali F. 2020b. Identification of culturable petroleum-degrading bacteria and fungi from petroleum-contaminated sites in Brunei Darussalam. Journal of Environmental Science and Health, Part A 55 (13): 1542–1547. DOI: 10.1080/10934529.2020.1826238
Yakop F, Taha H, Shivanand P. 2019. Isolation of fungi from various habitats and their possible bioremediation. Current Science 116 (5): 733–740. DOI: 10.18520/cs/v116/i5/733-740
Yue Y, Yu H, Li R, Xing R, Liu S, Li P. 2015. Exploring the antibacterial and antifungal potential of jellyfish-associated marine fungi by cultivation-dependent approaches. PLoS ONE 10 (12): e0144394. DOI: 10.1371/journal.pone.0144394
Zakaria L, Yee TL, Zakaria M, Salleh B. 2011. Diversity of microfungi in sandy beach soil of Teluk Aling, Pulau Pinang. Tropical Life Sciences Research 22 (1): 71–80.
Zhang L, An R, Wang J, Sun N, Zhang S, Hu J, Kuai J. 2005. Exploring novel bioactive compounds from marine microbes. Current Opinion in Microbiology 8 (3): 276–281. DOI: 10.1016/j.mib.2005.04.008.
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