Increasing ploidy level of marigold (Tagetes patula Sudamala Barak) using colchicine with acute and chronic methods
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Abstract. Sari YN, Aisyah SI, Sukma D, Syukur M. 2025. Increasing ploidy level of marigold (Tagetes patula Sudamala Barak) using colchicine with acute and chronic methods. Biodiversitas 26: 2726-2734. Marigold (Tagetes sp.) is a popular and potential ornamental plant in Indonesia, especially in Bali, commonly used in religious ceremonies and industry. A significant challenge in the floriculture industry is producing plants with desirable traits, such as larger flowers. Sudamala Barak has dark red flowers with small size (4,5-5,6 cm), but consumers prefer larger ones (8-10 cm). Colchicine is a chemical mutagen that disrupts spindle fiber formation during mitosis, thereby inducing chromosome doubling and resulting in polyploid cells. Tagetes patula is an allotetraploid species (2n = 48). This study aimed to determine the LC50 value and analyze the morphological, histological, and cytological characteristics of Sudamala Barak mutants. The research was conducted from May 2024 to February 2025 using two application methods: acute (seven concentrations) and chronic (four concentrations) based on LC50 (lethal concentration 50%). The results showed that colchicine significantly affected various plant characteristics. The LC50 value was found to be 0.346%. Overall, the chronic method produced greater diversity than the acute method. Colchicine treatment influenced plant height, canopy width, number of primary branches, stem diameter, flowering time, and flower diameter. It also increased the number of stomata and chloroplasts, potentially improving photosynthetic efficiency. In the acute method, the highest ploidy level was euploid-pentaploid (2n = 60) at concentrations of 0.05%; 0.1%; 0.15%; 0.25%; and 0.3%. In contrast, in the chronic method, the highest ploidy level was euploid-autoalooctaploid (2n = 96) at 10× and 20× LC50 dilution concentrations.
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References
Afifah UAN, Wiendi NMA, Maharijaya A. 2020. In vitro polyploidy induction of patchouli (Pogostemon Cablin Benth.) by colchicine. J Trop Crop Sci 7 (1): 37-44. DOI: 10.29244/jtcs.7.01.37-44.
Aisyah SI, Saraswati RAM, Yudha YS, Nurcholis W. 2022a. The diversity of agromorphological characters of Portulaca grandiflora in the MV8 population deriving from recurrent irradiation. Biodiversitas 23 (9): 4432-4439. DOI: 10.13057/biodiv/d230908.
Aisyah SI, Yudha YS, Sukma D, Nurcholis W. 2022b. Phenotypic variation and the polyphenols content alteration of Celosia cristata due to chronically induced mutation using ethyl methane sulphonate. J Southwest Jiaotong Univ 57 (3): 221-230. DOI: 10.35741/issn.0258-2724.57.3.18.
Aisyah SI, Yudha YS, Sukma D, Nurcholis W. 2023. The analysis of morphological diversity and polyphenols content of Celosia cristata in M2 population induced by ethyl methane sulphonate. J Trop Life Sci 13 (1): 115-122. DOI: 10.11594/jtls.13.01.11.
Anggraeni L, Sulistyaningsih E, Purwantoro A. 2023. Chromosome variation and karyomorphology of Artemisia cina O. Berg: Comparison on colchicine and 2, 4-D+ BA treatment. Res Sq 2023: 1-17. DOI: 10.21203/rs.3.rs-3646575/v1.
Ansari E, Khosrowshahli M, Etminan A, Jafari AA. 2022. Polyploidy induction and ploidy level determination in annual and perennial diploid Medicago species using the enumeration of chloroplasts of stomata guard cells. Cytol Genet 56: 164-171. DOI: 10.3103/S0095452722020025.
Avdi? J, Sarajli? N, Be?i? B, Murti? S, Turalija A. 2013. The effect of concentration, exposure time and type of colchicine treatment on the morphological characteristics of French marigold (Tagetes patula L. var. nana). 2013 (2): 353-358.
Bat H, Alt?nda? FN, Yi?it MA, Ellialt?o?lu ??, Çömlekçio?lu N. 2021. Ploidy estimation in pepper and eggplant via stomata characteristics. Intl J Agric For Life Sci 5 (2): 11-18.
Bhattarai K, Kareem A, Deng Z. 2021. In vivo induction and characterization of polyploids in Gerbera daisy. Sci Hortic 282: 110054. DOI: 10.1016/j.scienta.2021.110054.
Buchori A, Sanjaya IPW, Putra RP, Sukma D, Suprapta DN, Syukur M, Nurcholis W, Aisyah SI. 2024. Assessment of Tagetes patula mutants and its wild type for flower morphology, polyphenol contents, and antioxidant activity. Sabrao J Breed Genet 56 (3): 1147-1158. DOI: 10.54910/sabrao2024.56.3.21.
Cabahug RAM, Khanh HTTM, Lim K-B, Hwang Y-J. 2021. Phenotype and ploidy evaluation of colchicine-induced Echeveria ‘Peerless’. Toxicol Environ Health Sci 13: 17-24. DOI: 10.1007/s13530-020-00069-z.
Chinnathambi V, Panwar S, Singh KP, Namita, Lekshmy S, Mallick N, Mehraj U. 2024. In vitro androgenesis for isolation of haploid and ploidy analysis in marigold (Tagetes patula L.). Sci Hortic 330: 112962. DOI: 10.1016/j.scienta.2024.112962.
Cristina U, Sala F. 2023. Flowers color variation in Tagetes patula in relation to the substrate and time during the flowering period. J Hortic For Biotechnol 27 (3): 23-31.
Dewi IAP, Pharmawati M. 2018. Penggandaan kromosom marigold (Tagetes erecta L.) dengan perlakuan kolkisin. Jurnal Ilmu Biologi Biosfer 35: 153-157. DOI: 10.20884/1.mib.2018.35.3.773. [Indonesian]
Eng W-H, Ho W-S. 2019. Polyploidization using colchicine in horticultural plants: A review. Sci Hortic 246: 604-617. DOI: 10.1016/j.scienta.2018.11.010.
Fakhrzad F, Jowkar A, Shekafandeh A, Kermani MJ, Moghadam A. 2023. Tetraploidy induction enhances morphological, physiological and biochemical characteristics of wallflower (Erysimum cheiri (L.) Crantz). Sci Hortic 308: 111596. DOI: 10.1016/j.scienta.2022.111596.
Farhadi N, Panahandeh J, Motallebi-Azar A, Mokhtarzadeh S. 2023. Production of autotetraploid plants by in vitro chromosome engineering in Allium hirtifolium. Hortic Plant J 9 (5): 986-998. DOI: 10.1016/j.hpj.2022.12.013.
Fathurrahman F, Mardaleni, Krisianto A. 2023. Effect of colchicine mutagen on phenotype and genotype of Vigna unguiculata var. sesquipedalis the 7th generation. Biodiversitas 24 (3): 1408-1416. DOI: 10.13057/biodiv/d240310.
Ghanbari MA, Jowkar A, Salehi H, Zarei M. 2019. Effects of polyploidization on petal characteristics and optical properties of Impatiens walleriana (Hook.). Plant Cell Tiss Org Cult 138: 299-310. DOI: 10.1007/s11240-019-01625-3.
Gupta G, Memon AG, Pandey B, Khan MS, Iqbal MS, Srivastava JK. 2021. Colchicine induced mutation in Nigella sativa plant for the assessment of morpho-physiological and biochemical parameter Vis-A-Vis in vitro anti-inflammatory activity. Open Biotechnol J 15 (1): 173-182. DOI: 10.2174/1874070702115010173.
Haring F, Farid M, Sudirman S, Anshori MF. 2023. The morpho-somatic and chromosomal changes in colchicine polyploidy induction Colocasia esculenta var. antiquorium. Plant Breed Biotechnol 11 (2): 105-116. DOI: 10.9787/PBB.2023.11.2.105.
Iannicelli J, Guariniello J, Tossi VE, Regalado JJ, Di Ciaccio L, Van Baren CM, Álvarez SIP, Escandón AS. 2020. The “polyploid effect” in the breeding of aromatic and medicinal species. Sci Hortic 260: 108854. DOI: 10.1016/j.scienta.2019.108854.
Ji X, Lelivelt C, Wijnker E, de Jong H. 2022. Is partial desynapsis in cauliflower (Brassica oleracea L. var. botrytis) pollen mother cells linked to aneuploidy in the crop?. Euphytica 218: 79. DOI: 10.1007/s10681-022-03027-7.
Karagöz FP, Dursun A, Dumlu B, Kara?al M, Karagöz H. 2024. Colchicine-induced polyploidy and morphological changes in wild Silene compacta Fischer: Potential as an ornamental plant in Türkiye. J Biometry Stud 4 (2): 79-90. DOI: 10.61326/jofbs.v4i2.04.
Kishimoto S, Oda-Yamamizo C, Ohmiya A. 2019. Comparison of petunia and calibrachoa in carotenoid pigmentation of corollas. Breed Sci 69 (1): 117-126. DOI: 10.1270/jsbbs.18130.
Kruthika HS, Rukmangada MS, Naik VG. 2023. Genome size, chromosome number variation and its correlation with stomatal characters for assessment of ploidy levels in a core subset of mulberry (Morus spp.) germplasm. Gene 881: 147637. DOI: 10.1016/j.gene.2023.147637.
Kurniati F. 2021. Potensi bunga marigold (Tagetes erecta L.) sebagai salah satu komponen pendukung pengembangan pertanian. Media Pertanian 6 (1): 22-29. DOI: 10.37058/mp.v6i1.3010. [Indonesian]
Kushwah KS, Verma RC, Patel S, Jain NK. 2018. Colchicine induced polyploidy in Chrysanthemum carinatum L. J Phylogenetics Evol Biol 6 (1): 1000193. DOI: 10.4172/2329-9002.1000193.
Lenawaty DY, Sukma D, Syukur M, Suprapta DN, Nurcholis W, Aisyah SI. 2022. Increasing the diversity of marigold (Tagetes sp.) by acute and chronic chemical induced mutation of EMS (Ethyl Methane Sulfonate). Biodiversitas 23: 1399-1407. DOI: 10.13057/biodiv/d230326.
Li J, Chen J, Zhang Q, Yu P, Zhou Y, Jia G. 2022. The composition of anthocyanins and carotenoids influenced the flower color heredity in Asiatic hybrid lilies. Horticulturae 8 (12): 1206. DOI: 10.3390/horticulturae8121206.
Li Y, Feng J, Zheng L, Huang J, Yang Y, Li X. 2020. Intercropping with marigold promotes soil health and microbial structure to assist in mitigating tobacco bacterial wilt. J Plant Pathol 102: 731-742. DOI: 10.1007/s42161-020-00490-w.
Mahanta M, Gantait S, Sarkar S, Sadhukhan R, Bhattacharyya S. 2023. Colchicine-mediated in vitro polyploidization in Gerbera hybrid. 3 Biotech 13: 74. DOI: 10.1007/s13205-022-03457-z.
Makin FMPR, Welsiliana, Wiguna GA. 2022. Karakterisasi stomata dan trikomata daun kirinyuh (Chromolaena odorata L.). Sci-Bio: J Sci Biodivers 3 (1): 61-67. DOI: 10.32938/jsb/vol3i1pp61-67. [Indonesian]
Manzoor A, Ahmad T, Bashir MA, Hafiz IA, Silvestri C. 2019. Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. Plants 8 (7): 194. DOI: 10.3390/plants8070194.
Mo L, Chen J, Lou X, Xu Q, Dong R, Tong Z, Huang H, Lin E. 2020. Colchicine-induced polyploidy in Rhododendron fortunei Lindl. Plants 9 (4): 424. DOI: 10.3390/plants9040424.
Patel S. 2023. Marigold flower blooming stage detection in complex scene environment using faster RCNN with data augmentation. J Adv Comp Sci Appl 14 (3): 676-684. DOI: 10.14569/ijacsa.2023.0140379.
Prasath D, Nair RR, Babu PA. 2022. Effect of colchicine induced tetraploids of ginger (Zingiber officinale Roscoe) on cytology, rhizome morphology, and essential oil content. J Appl Res Med Aromat Plants 31: 100422. DOI: 10.1016/j.jarmap.2022.100422.
Rahmawati AAN, Nandariyah, Parjanto. 2024. Phenotypic performance of Srikayang's shallot variety M1 by colchicine induction. Biodiversitas 25 (3): 1297-1303. DOI: 10.13057/biodiv/d250346.
Roy S, Thakur A, Singh R, Kumar A, Yadav S. 2023. Determination of semi-lethal dose of colchicine on in vitro grown callus of Azadirachta indica genotypes. Appl Biol Chem J 4 (1): 28-33. DOI: 10.52679/tabcj.2023.0003.
Sajjad YA, Jaskani MJ, Mehmood A, Ahmad I, Abbas H. 2013. Effect of colchicine on in vitro polyploidy induction in African marigold (Tagetes erecta). Pak J Bot 45 (3): 1255-1258.
Samadi N, Naghavi MR, Moratalla-López N, Alonso GL, Shokrpour M. 2022. Morphological, molecular and phytochemical variations induced by colchicine and EMS chemical mutagens in Crocus sativus L. Food Chem 4: 100086. DOI: 10.1016/j.fochms.2022.100086.
Sanaei-Hoveida Z, Mortazavian SMM, Norouzi M, Sadat-Noori SA. 2024. Exploring the potential of polyploidization as a breeding tool for medicinal plants: A case study on cumin (Cuminum cyminum L.). Plant Cell Tiss Org Cult 156 (1): 23. DOI: 10.1007/s11240-023-02648-7.
Sattler MC, Carvalho CR, Clarindo WR. 2016. The polyploidy and its key role in plant breeding. Planta 243 (2): 281-296. DOI: 10.1007/s00425-015-2450-x.
Song Z, Zuo Y, Li W, Dai S, Liu G, Pu Z, Yan Z. 2024. Chromosome stability of synthetic Triticum turgidum–Aegilops umbellulata hybrids. BMC Plant Biol 24: 391. DOI: 10.1186/s12870-024-05110-8.
Susrama IGK, Yuliadhi KA. 2020. Induced mutagenesis in yellow flowering marigold with colchicine in hydrogen peroxide. Adv Trop Biodivers Environ Sci 4: 44-48. DOI: 10.24843/atbes.2020.v04.i02.p04.
Syukur M, Sastrosumarjo S, Wahyu Y, Aisyah SI, Sujiprihati S, Yunianti R. 2019. Sitogenetika Tanaman. IPB Press, Bogor. [Indonesian]
Tammu RM, Nuringtyas TR, Daryono BS. 2021. Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia. J Genet Eng Biotechnol 19 (1): 31. DOI: 10.1186/s43141-021-00131-4.
Teixeira M, Tao W, Fernandes A, Faria A, Ferreira IMPLVO, He J, de Freitas V, Mateus N, Oliveira H. 2023. Anthocyanin-rich edible flowers, current understanding of a potential new trend in dietary patterns. Trends Food Sci Technol 138: 708-725. DOI: 10.1016/j.tifs.2023.07.010.
Towner JW. 1961. Cytogenetic studies on the origin of Tagetes patula. I. meiosis and morphology of diploid and allotetraploid T. erecta× T. tenuifolia. Am J Bot 48 (9): 743-751. DOI: 10.1002/j.1537-2197.1961.tb11706.x.
Towner JW. 1962. Cytogenetics of Tagetes jaliscensis x T. erecta. Am J Bot 49 (10): 1064-1067. DOI: 10.1002/j.1537-2197.1962.tb15047.x.
Udofia EG, Daudu OY, Isong AE, Odeyemi F, Adeboye SE, Adeshina DA, Osisami OF, Joseph RI. 2024. The effects of colchicine on the mitotic behaviour of selected indigenous tomato (Solanum lycopersicum L.) accession. Badeggi J Agric Res Environ 6 (2): 80-88. DOI: 10.35849/bjare202402/184/008.
Watts A, Bondada R, Maruthachalam R. 2023. Identification of Arabidopsis thaliana haploid plants by counting the chloroplast numbers in stomatal guard cells. Plant Physiol Rep 28: 180-184. DOI: 10.1007/s40502-022-00706-4.
Wibisono K, Aisyah SI, Nurcholis W, Suhesti S. 2022. Sensitivity in callus tissue of Plectranthus amboinicus (L.) through mutation induction with colchicine. Agrivita J Agric Sci 44 (1): 82-95. DOI: 10.17503/agrivita.v44i1.3058.
Widoretno W, Azriningsih R, Sukmadjaja D, Rosyidah M. 2023. In vitro induction and identification of polyploid Amorphophallus muelleri Blume plants by colchicine treatment. Agrivita J Agric Sci 45 (1): 87-97. DOI: 10.17503/agrivita.v45i1.3992.
Yamagishi M. 2020. White with partially pink flower color in Lilium cernuum var. album is caused by transcriptional regulation of anthocyanin biosynthesis genes. Sci Hortic 260: 108880. DOI: 10.1016/j.scienta.2019.108880.
Yan Y-J, Qin S-S, Zhou N-Z, Xie Y, He Y. 2022. Effects of colchicine on polyploidy induction of Buddleja lindleyana seeds. Plant Cell Tiss Org Cult 149: 735-745. DOI: 10.1007/s11240-022-02245-0.
Yirga M. 2021. Poliploidy and its implications in plants breeding-A review. Intl J Curr Res Biosci Plant Biol 8 (3): 1-9. DOI: 10.20546/ijcrbp.2021.803.001.