Morphological response and genetic variability of four species of chili pepper (Capsicum spp.) under infection of pepper yellow leaf curl virus




Abstract. Sayekti TWDA, Syukur M, Hidayat SH, Maharijaya A. 2021. Morphological response and genetic variability of four species of chili pepper (Capsicum spp.) under infection of pepper yellow leaf curl virus. Biodiversitas 22: 4758-4765. Chili pepper has various types and species, but only five known species are commonly used and consumed. Most cultivated chili is susceptible to various plant diseases, one of which is Pepper yellow leaf curl disease (PYLCD) caused by Pepper yellow leaf curl virus (PYLCV) (Begomovirus, Geminiviridae). To control PYLCD, resistant variety assembly is required to prevent virus infection in cultivated plants. From this research, testing on four chili species is expected to provide information regarding the resistance and performance of chili peppers to conditions infected with PYLCV. This study was conducted at Dramaga Bogor, West Java, Indonesia in two experimental units: planting under virus-free conditions (as control) and virus-infected conditions. Each experimental unit was carried out using a single factor Randomized Complete Block Design (RCBD) with three replications. Twenty-nine genotypes of chili pepper were used consisted of four species, including C. annuum, C. frutescens, C. chinense, and C. baccatum. Of the 29 genotypes tested, thirteen genotypes in the resistant, nine genotypes in moderate resistant, two genotypes in moderate susceptible, three genotypes in the Susceptible, and two genotypes in the highly susceptible category. The heritability, genotypic coefficient of variance (GCOV) and phenotypic coefficient of variance (PCOV) value obtained from testing for all characters is high, ranging from 65.16-99.12%, 14.87-82.60%, and 15.77-84.45%, respectively. Most of the genotypes from C. chinense showed good resistance to PYLCV. In general, by considering the category of the resistance level and other characters such as productivity, ‘Jolokia’ (C. chinense), ‘Anies’ (C. annuum) and ‘Bonita’ (C. frutescens) can be ascertained as potential candidate sources of resistance to PYLCV.


Adhikari BN, Joshi BP, Shrestha J, Bhatta NR. 2018. Genetic variability, heritability, geneic advance and correlation among yield and yield component of rice (Oryza sativa L.). J. Agric Nat Resour 1(1):149-160.
Adluri PK, Baldoldiya GM, Nath PD. 2017. Screening of bhut jolokia (Capsicum chinense Jacq.) germplasm of North East India against chilli leaf curl virus. IJPAB. 5(4): 1189-1196. DOI: 10.18782/2320-7051.5624
Ajith PM, Manju P. 2006. Genetic variation for yield and anthracnose resistance in chilli (Capsicum annuum L.). Indian J, Genet 66(2): 161-162.
Al-Basir F, Adhurya S, Banerjee M, Venturino E, Ray S. 2020. Modelling the effect of incubation and latent periods on the dynamics of vector-borne viral disease. Bull Math Biol. 82(94): 1-22. DOI: 10.1007/s11538-020-00767-2
Barchenger DW, Yule S, Jeeatid N, Lin SW, Wang YW, Lin TH, Chan YL, Kenyon L. 2019. A novel source of resistance to pepper yellow leaf curl Thailand virus (PepYLCThV) (Begomovirus) in chile pepper. HortScience. 54(12): 2146-2149. DOI: 10.21273/HORTSCI14484-19
Beam K, Ascencio-Ibanez. 2020. Geminivirus resistance: a minireview. Front. Plant Sci 11: 1-9. DOI: 10.3389/fpls.2020.01131
Blair MW, Morales FJ. 2008. Geminivirus resistance breeding in common bean. In: Prespectives in Agriculture Veterinary Science, Nutrition and Natural Resources. CABI Wallingford.
Ganefianti DW. 2010. Genetics of Resistance on Chilli Pepper to Yellow Leaf Curl Begomovirus and Strategy Breeding. [Disertation]. Bogor Agricultural University, Bogor. [Indonesian]
Garcia-Gano E, Resende RO, Fernandez-Munoz R, Moriones E. 2006. Synergistic interaction between tomato chlorosis virus and tomato spotted wilt virus result in breakdown of resistance in tomato. Virology 96(11): 1263-1269. DOI: 10.1094/PHYTO-96-1263
Gaswanto R, Syukur M, Hidayat SH, Gunaeni N. 2016. Symptom and host range identification of six Chilli Begomovirus isolate in Indonesia. J. Hort. 26(2): 223-234. [Indonesian]
Gomez AK, Gomez AA. 1987. Statitically Procedures for Agricultural Research Second Edition. John Willey and Sons. Inc. Canada.
Jarret RL, Barboza GE, Batista FRC, Berke T, Chou YY, Hulse-Kemp A, Ochoa-Alejo N, Tripodi P, Veres A, Garcia CC et al. 2019. Capsicum – an abbreviated compendium. J. Ammer. Soc. Hort. Sci. 144(1): 3-22.
Johnson WW, Robinson HF, Comstock RE. 1955. Genotypic and phenotypic correlations in soybeans and their implications in selection. Agron. J. 47: 477-482.
Lopez-Fabuel I, Wetzel T, Bertolini E, Bassler A, Vidal E, Torres LB, Yuste A, Olmos A. 2013. Real-time multiplex RT-PCR for the simultaneous detection of the five main grapevine viruses. J. Virol. Methods. 188: 21-24. DOI: 10.1016/j.jviromet.2012.11.034
Lush JL. 1949. Heritability of quantitative characters in farm animals. Hereditas 35:356-375.
Mauck K, Bosque-Perez NA, Eigenbrode SD, De Moraes CM, Mescher MC. 2012. Transmission mechanism shape pathogen effects on host-vector interaction: evidence from plant viruses. Funct. Ecol 26:1162-1175.
Miras M, Sempere RN, Kraft JJ, Miller WA, Aranda A, Truniger V. 2014. Interfamilial recombination between viruses led to acquisition of a novel translation-enhancing RNA element that allows resistance breaking. New Phytol. 202:233-246. DOI: 10.1111/nph.12650
Mohamed EF. 2010. Interaction between some viruses which attack tomato (Lycopersicon esculentum Mill.) plants and their effect on growth and yield of tomato plants. Am. J. Sci. 6(8): 311-320
Mongkolporn O, Taylor PWJ. 2011. Capsicum. In: Kole C (ed). Wild Crop Relatives: Genomic and Breeding Resources Vol : Vegetables 1st Edn. Springer, New York.
Munoz-Concha D, Quinones X, Hernandez JP, romero S. 2020. Chilli pepper landrace survival and family farmers in central chile. Agronomy 10: 1541-1558.
Nankar AN, Todorova V, Tringovska I, Pasev G, Radeva-Ivanova V, Ivanova V, Kostova D. 2020. A step towards Balkan Capsicum annuum L. core collection:phenotypic and biochemical characterization of 180 accessions for agronomic fruit quality, and virus resistance traits. PLoS ONE. 15(8): e0237741.
Paul S, Kumar N, Kumar A. 2017. Characterization and genetic variation study among linseed (Linium usitatissimum L.) genotypes for seed yield and related traits in Mid-Hills of North-West Himalayas. Plant Arch. 17(1): 407-411.
Pena-Yam LP, Munoz-Ramirez LS, Aviles-Vinas SA, Canto-Flick A, Perez-Pastrana J, Guzman-Antonio A, Santana-Buzzy N. 2019. Analysis of genetic parameters of habanero pepper (Capsicum chinense Jacq.) in Yucatan, Mexico. Hort Science 54(3): 429-433.
Rimbaud L, Dallot S, Delaunay A, Borron S, Soubeyrand S, Thebaud G, Jacquot E. 2015. Assessing the mismatch between incubation and latent periods for vector-borne disease:the case of Sharka. Phytopathology 105(11). 1408-1416. DOI: 10.1111/nph.12650
Rubio L, Galipienso L, Ferriol I. 2020. Detection of plant viruses and disease management: relevance of genetic diversity and evolution. Front. Plant Sci. 11:1092-1114. DOI: doi: 10.3389/fpls.2020.01092
Seprico D. 2020. Beyond quantitative and qualitative traits: three telling cases in the life sciences. Biol Philos. 35(3): 34-59. DOI: 10.1007/s10539-020-09750-6
Singh KA, Kushwaha N, Chakraborty S. 2016. Synergistic interaction among begomoviruses leads to the suppression of host defense-related gene expression and breackdown of resistance in chilli. Appl Microbiol Biotechnol. 100(9): 4035-4049. DOI: 10.1007/s00253-015-7279-5
Steel RGD, Torrie JH. 1981. Principles and Procedures of Statistics: A Biometrical Approach. 2nd Ed. McGrwa Hill Book, New York.
Sulandari S, Hidayat SH, Suseno R, Harjosudarmo J, Sosromarsono S. 2007. Inoculation of pepper yellow leaf curl virus on various plants and detection of the virus in its insect vector Bemisia tabaci Genn. (Hemiptera: Aleyrodidae). Proceeding of The Third Asian Conference on Plant Pathology. Gajah Mada University 20-24 August 2007.
Sulandari S, Suseno R, Hidayat SH, Harjosudarmo J, Sosromarsono S. 2006. Detection and host range study of virus associated with pepper yellow leaf curl disease. Hayati 13(1): 1-6. [Indonesian]
Terfa GN, Gurmu GN. 2020. Genetic variability, heritability and genetic advance in lindseed (Linum usitatissium L.) genotypes for seed yield and other agronomic traits. Oil Crop Sci. 5:156-160. DOI: 10.1016/j.ocsci.2020.08.002
Usman MG, Rafii MY, Ismail MR, Malek MA, Latif MA. 2014. Heritability and genetic andvance among chili pepper genotypes for heat tolerance and morphophysiological characteristics. Sci. World J. 2014:308042.
Usman MG, Rafii MY, Martini MY, Oladosu Y, Pedram K. 2017. Genotypic character relationship and phenotypic path coefficient analysis in chili pepper genotypes grown under tropical condition. J Sci Food Agric 97(4):1164-1171.
Whirter KS. 1979. Breeding of cross-pollinated crops. In: Knight. R (ed). A Course Manual in Plant Breeding. Australian Vide-Chanceller’s Committee. Brisbanen.
Yan Z, Perez-de-Castro A, Diez MJ, Hutton SF, Visser RG, Wolters AMA, Bai Y, Li J. 2018. Resistance to tomato yellow leaf curl virus in tomato genrmplasm. Front. Plant Sci. 9:1-14. DOI: 10.3389/fpls.2018.0119
Zaidi SSA Naqvi RZ, Asif M, Strickler S, Shakir S, Shafiq M, Khan AM, Amin I, Mishra B, Mukhtar MS et al. 2019. Molecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton (Gossypium hirsutum). Plant Biotechnol J (2019): 1-16. DOI: 10.1111/pbi.13236

Most read articles by the same author(s)

1 2 > >>