Characterization and biocontrol of Pantoea ananatis, the causal agent of rice bacterial leaf blight in Malang, Indonesia
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Abstract. Andriani LT, Poromarto SH, Supyani, Purwanto E, Hadiwiyono. 2025. Characterization and biocontrol of Pantoea ananatis, the causal agent of rice bacterial leaf blight in Malang, Indonesia. Biodiversitas 26: 1537-1544. Disease outbreaks pose a significant obstacle to sustainable agricultural production. Two major diseases affecting rice crops are bacterial leaf blight and blast disease. Bacterial leaf blight caused by Pantoea ananatis is considered new, based on field observations, the cause of bacterial leaf blight is still Xanthomonas oryzae pv. oryzae. Research on P. ananatis is important to provide new knowledge about the causes of bacterial leaf blight other than X. oryzae pv. oryzae. The symptoms of bacterial leaf blight include yellowing and browning of the leaf, followed by drying, resulting in death of the leaf cells. The objectives of this study were to isolate the bacteria, identify them morphologically and molecularly, determine physiological characteristics and explore their biocontrol potential using rhizobacteria. Bacterial characterization was performed both morphologically and physiologically. This study determined the hypersensitive response of tobacco leaves, physiological and biochemical characteristics, pathogenicity tests, and molecular identification via 16S rRNA gene sequencing. The bacteria formed yellow colonies on yeast chalk dextrose agar at 36°C. Isolate was positive for indole and Levan tests, used citrate as a carbon source, and liquefied gelatin at low temperatures. The bacteria showed positive results in tobacco hypersensitivity and pathogenicity tests on paddy rice. Molecular identification results showed that the bacterial pathogen was identified as P. ananatis. The result of biocontrol potential test was shown that bacteria with the code UNS-R2 can control the pathogenic bacteria P. ananatis using spray method. These findings can be used as new knowledge about the types of leaf blight pathogens other than X. oryzae and their control alternatives to determine tactical steps in integrated crop management toward sustainable agriculture. This is the first report on the identification and characterization of P. ananatis in rice in Indonesia and the biocontrol potential using rhizobacteria.
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References
Abdel-Gaied TG, Abd El-Khair H, Youssef MM, El-Maaty SA, Mikhail MS. 2022. First report of strawberry bacterial leaf blight caused by Pantoea ananatis in Egypt. J Plant Prot Res 62 (2): 207-214. DOI: 10.24425/jppr.2022.141359.
Agrios GN. 2016. Plant Pathology (Fifth Edition). Academic Press, Amsterdam. DOI: 10.1016/C2009-0-02037-6.
Aini, LQ, Trianti, I, Rachmawati, SW, Putra, AM, Anastasya, NA, and Setiawan, A. 2023. In vitro characterization of UB Forest (Malang, Indonesia) indigenous bacteria as plant growth promoting bacteria (PGPB). Biodiversitas 24: 4558-4565. DOI: 10.13057/biodiv/d240837.
Aksoy HM, Boluk E. 2019. First report of Pantoea ananatis in japonica rice varieties in Turkey. J Plant Pathol 101: 409. DOI: 10.1007/s42161-018-0178-8.
Alawiye TT, Babalola OO. 2019. Bacterial diversity and community structure in typical plant rhizosphere. Diversity 11 (10): 179. DOI: 10.3390/d11100179.
Amaria W, Sinaga MS, Mutaqin KH, Supriadi, Widodo. 2023. Hemolysis and hypersensitive tests ease culture collection management of antagonistic bacteria. J Trop Plant Pests Dis 23 (2): 24-30. DOI: 10.23960/jhptt.22324-30.
Azizi MMF, Zulperi D, Rahman MAA, Abdul-Basir B, Othman NA, Ismail SI, Hata EM, Ina-Salwany MY, Abdullah MAF. 2019. First report of Pantoea ananatis causing leaf blight disease of rice in Peninsular Malaysia. Plant Dis 103 (8): 1-2 DOI: 10.1094/PDIS-01-19-0191-PDN.
BSIP [Badan Standardisasi Instrumen Pertanian]. 2009. Pengelolaan Tanaman Terpadu (PTT) Padi Sawah. Balai Besar Penelitian Tanaman Padi. Available at https://pustaka.setjen. pertanian.go.id/informasi-pub/category/6-padi?download=171:padi&start=40. [Indonesian]
Cappucino JG, Welsh CT. 2020. Microbiology A laboratory manual 12th edition. Pearson Education, Inc., New York.
Cother E, Reinke R, McKenzie C, Lanoiselet VM, Noble DH. 2004. An unusual stem necrosis of rice caused by Pantoea ananas and the first record of this pathogen on rice in Australia. Australas Plant Pathol 33: 495-503. DOI: 10.1071/AP04053.
De Armas S, Galván GA, Lapaz MI, González-Barrios P, Vicente E, Pianzzola MJ, Siri MI. 2022. Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. Plant Dis 106 (4): 1216-1225. DOI: 10.1094/PDIS-06-21-1140-RE.
Doni F, Suhaimi NSM, Irawan B, Mohamed Z, Mispan MS. 2021. Associations of Pantoea with rice plants: As friends or foes?. Agriculture 11 (12): 1278. DOI: 10.3390/agriculture11121278.
Egorova, M, Mazurin, E, and Ignatov, AN. 2015. First report of Pantoea ananatis causing grain discoloration and leaf blight of rice in Russia. New Dis Rep 32: 21-21. DOI: 10.5197/j.2044-0588.2015.032.021.
Hou D, Lian T, Guo G, Gong H, Wu C, Han P, Weng S, He J. 2023. Integration of microbiome and Koch’s postulates to reveal multiple bacterial pathogens of whitish muscle syndrome in mud crab, Scylla paramamosain. Microbiome 11 (1): 155. DOI: 10.1186/s40168-023-01570-6.
Janse JD. 2006. Phytobacteriology: Principles and Practice. CABI Publishing, Wallingford. DOI: 10.1079/9781845930257.0000.
Javandira C, Aini LQ, Sugiharto AN, Abadi AL. 2013. The potential of Bacillus sp. and Pseudomonas sp. as biological control agents against corn leaf blight disease caused by Pantoea sp. Agrivita 35 (2): 103-109. DOI: 10.17503/Agrivita-2013-35-2-p103-109.
Jin P, Tan Z, Wang H, Liu W, Miao W. 2021. Antimicrobial effect of Bacillus licheniformis HN-5 bacitracin A on rice pathogen Pantoea ananatis. BioControl 66: 249-257. DOI: 10.1007/s10526-020-10052-9.
Kini K, Agnimonhan R, Afolabi O, Milan B, Soglonou, B, Gbogbo V, Koebnik R, Silué D. 2017. First report of a new bacterial leaf blight of rice caused by Pantoea ananatis and Pantoea stewartii in Togo. Plant Dis 101: 241-242. DOI: 10.1094/PDIS-06-16-0940-PDN.
Kini K, Dossa R, Dossou B, Mariko M, Koebnik R, Silué D. 2019. A semi-selective medium to isolate and identify bacteria of the genus Pantoea. J Gen Plant Pathol 85: 424-427. DOI: 10.1007/s10327-019-00862-w.
Kini K. 2020. Pantoea spp: A new bacterial threat to rice production in Sub-Saharan Africa. [Dissertation]. Universitas Montpellier, France.
Luna E, Lang JM, McClung A, Wamishe Y, Jia Y, Leach JE. 2023. First report of new bacterial leaf blight of rice caused by Pantoea ananatis United States. Plant Dis 107 (7): 2214. DOI: 10.1094/PDIS-08-22-2014-PDN.
Mamede MC, Tebaldi ND, Mota LCBM, Martins OM, Coelho L. 2018. Detection of Pantoea ananatis in corn seeds on semi-selective medium. Trop Plant Pathol 43: 254-256. DOI: 10.1007/s40858-017-0203-z.
Mondal KK, Mani C, Singh J, Kim J-G, Mudgett MB. 2011. A new leaf blight of rice caused by Pantoea ananatis in India. Plant Dis 95 (12): 1582. DOI: 10.1094/PDIS-06-11-0533.
Mwangi RW, Mustafa M, Charles K, Wagara IW, Kappel N. 2023. Selected emerging and reemerging plant pathogens affecting the food basket: A threat to food security. J Agric Food Res 14: 100827. DOI: 10.1016/j.jafr.2023.100827.
Nurjanah N, Joko T, Subandiyah S. 2018. Characterization of Pantoea ananatis isolated from garlic and shallot. Jurnal Perlindungan Tanaman Indonesia 21 (2): 120. DOI: 10.22146/jpti.27407.
Rahma H, Nurbailis, Kristina N. 2019. Characterization and potential of plant growth-promoting rhizobacteria on rice seedling growth and the effect on Xanthomonas oryzae pv. oryzae. Biodiversitas 20 (12): 3654-3661. DOI: 10.13057/biodiv/d201226.
Reddy PP. 2014. Plant Growth Promoting Rhizobacteria for Horticultural Crop Protection. Springer, New Delhi. DOI: 10.1007/978-81-322-1973-6.
Reshma TS, Balan S, Dileep C. 2022. First report of rice grain discolouration and leaf blight caused by Pantoea ananatis in the Kuttanad agro-ecosystem, Kerala, India. Can J Plant Pathol 45 (1): 30-34. DOI: 10.1080/07060661.2022.2096697.
Sahni S, Prasad BD. 2021. Rapid methods for identification of plant-growth-promoting rhizobacteria. Intl J Plant Soil Sci 33 (15): 12-17. DOI: 10.9734/ijpss/2021/v33i1530506.
Schaad NW, Jones JB, Chun W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. American Phytopathological Society Press, Saint Paul. DOI: 10.1046/j.1365-3059.2001.00635.x.
Singh BK, Delgado-Baquerizo M, Egidi E, Guirado E, Leach JE, Liu H, Trivedi P. 2023. Climate change impacts on plant pathogens, food security and paths forward. Nat Rev Microbiol 21: 640-656. DOI: 10.1038/s41579-023-00900-7.
Toh WK, Loh PC, Wong H. 2019. First report of leaf blight of rice caused by Pantoea ananatis and Pantoea dispersa in Malaysia. Plant Dis 103 (7): 1764. DOI: 10.1094/PDIS-12-18-2299-PDN.
Tsaniyah B, Joko T, Widiastuti A. 2024. Identification of pathogens causing important diseases in leatherleaf fern (Rumohra adiantiformis) and in vitro inhibition using Bacillus velezensis B-27. Caraka Tani 39 (2): 297-310. DOI: 10.20961/carakatani.v39i2.83675.
US EPA [United States Environmental Protection Agency]. 2015. Climate Impacts on Agriculture and Food Supply: Climate Change Impacts. US Environmental Protection Agency, USA.
Yu L, Yang C, Ji Z, Zeng Y, Liang Y, Hou Y. 2021. First report of new bacterial leaf blight of rice caused by Pantoea ananatis in Southeast China. Plant Dis 106 (1): 310. DOI: 10.1094/PDIS-05-21-0988-PDN.