Distribution of banana Fusarium wilt in Banyumas, Indonesia, and characterization of F. oxysporum isolates from infected bananas and taro growing on the same farm

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DOI https://doi.org/10.13057/biodiv/d250401
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Vol. 25 No. 4 (2024)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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RUTH FETI RAHAYUNIATI ♥
Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman. Jl. Dr. Suparno, North Purwokerto, Banyumas 53122, Central Java, Indonesia
RULY EKO KUSUMA KURNIAWAN
Program Study of Conservation and Land Management, Faculty of Agriculture, Universitas Jenderal Soedirman. Jl. Dr. Suparno, North Purwokerto, Banyumas 53122, Central Java, Indonesia
ENDANG MUGIASTUTI
Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman. Jl. Dr. Suparno, North Purwokerto, Banyumas 53122, Central Java, Indonesia

Abstract

Abstract. Rahayuniati RF, Kurniawan REK, Mugiastuti E. 2024. Distribution of banana Fusarium wilt in Banyumas, Indonesia, and characterization of F. oxysporum isolates from infected bananas and taro growing on the same farm. Biodiversitas 25: 1344-1351. Fusarium wilt disease is a major constraint to banana production in Indonesia. The disease has also affected banana production in Banyumas, one banana-producing region. However, information on the distribution and characteristics of Fusarium wilt in Banyumas is limited. Growing mixed bananas and taro on the same farm in Banyumas is common. During the banana wilt survey, we found that taro also showed a symptom of Fusarium wilt. Therefore, this study aims to determine the distribution of Fusarium wilt disease on bananas in Banyumas, determine the characteristics of F. oxysporum on bananas and taro that grow on the same farm, and conduct pathogenicity tests of taro Fusarium isolates on bananas. The study consists of two steps, i.e., survey and sampling using purposive sampling technique conducted at the banana-growing area in Baturraden, Rawalo, Wangon, Purwojati, Gumelar, Ajibarang, Kemranjen, and Somagede districts. The second step involved the identification, characterization, and pathogenicity test of Fusarium isolate banana compared to isolate taro. A complete randomized design was used in an experimental study. All samples with Fusarium wilt symptoms were identified morphologically as comprised of macroscopic, microscopic characters and volatile odor of isolates. The results indicated that Fusarium wilt was equally distributed in Banyumas District, with the disease incidence reaching 50%. F. oxysporum isolates, which cause Fusarium wilt of banana and taro in Banyumas District, have indicated the same characteristics as Foc, i.e., diverse colony morphology, dominated by white to purple colonies with a cottony, smooth, and dry surface. Colonies are circular, irregular, dispersed, and undispersed on the surface of the medium with a filamentous margin; the single-septate oval-shaped microconidium and typical straight to sickle-shaped macroconidium. There were no significant differences between F. oxysporum isolate banana and taro in terms of disease incubation period, disease intensity, and Area Under Disease Progress Curve (AUDPC). Generally, this study found that Fusarium wilt was equally distributed in the lowlands. The Fusarium in bananas was the same as the Fusarium on taro in the same area, and the Fusarium isolated from taro effectively infected the banana.

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References
Aguilar-Hawod, K. G. I., de la Cueva, F. M., & Cumagun, C. J. R. (2020). Genetic diversity of fusarium oxysporum f. Sp. cubense causing Panama wilt of banana in the Philippines. Pathogens, 9(1), 1–24. https://doi.org/10.3390/pathogens9010032
Ata, H., Papuangan, N., & Bahtiar. (2016). Identifikasi cendawan patogen pada tanaman tomat (Solanum lycopersicum L.). Bioedukasi, 4(2), 541–550. https://doi.org/http://dx.doi.org/10.33387/bioedu.v4i2.168
Bebber, D. P. (2019). Climate change effects on Black Sigatoka disease of banana. Philosophical Transactions of the Royal Society B: Biological Sciences, 374(1775). https://doi.org/10.1098/rstb.2018.0269
BPS-Statistics of Banyumas Regency. (2022). Banyumas Regency in Figures 2022. BPS-Statistics of Banyumas Regency.
Cardillo, E., Acedo, A., & Abad, E. (2018). Topographic effects on dispersal patterns of Phytophthora cinnamomi at a stand scale in a Spanish heathland. PLoS ONE, 13(3), 1–23. https://doi.org/10.1371/journal.pone.0195060
Devika, B., Fatima, S., & Sonawane, B. N. (2017). Impact of physical factors on growth and sporulation of saprophytic pathogenic fungi. Int. J.Eng Dev and Res, 5(2), 1346–1350.
Dita, M., Echegoyén Ramos, P. E., & Pérez Vicente, L. F. (2013). Plan de Contingencia Ante un Brote de la Raza 4 Tropical de Fusarium oxysporum f. sp. cubense en un país de la región del OIRSA. OIRSA.
Dwivany, F. M., Wikantika, K., Sutanto, A., Ghazali, M. F., Lim, C., & Kemalesha, G. (2021). Pisang Indonesia. In ITB Press (Vols. 978-623–29, Issue March).
Firdausi, N., Hayati, A., & Rahayu, T. (2015). Studi Etnobotani dan Keragaman Pisang Buah ( Musaceae ) Pada Masyarakat Tradisional Pandalungan Desa Krai Kecamatan Yosowilangun Kabupaten Lumajang. Biosaintropis, 1(1), 26–34.
Gheorghe, B. A., Stelica, C., Relu, Z. C., & Maria, O. (2015). The biological growth parameters of the Fusarium oxysporum f. sp. glycines fungus. Romanian Biotechnological Letters, 20(6), 10921–10928.
Heck, D. W., Dita, M., Del Ponte, E. M., & Mizubuti, E. S. G. (2021). Incidence, spatial pattern and temporal progress of fusarium wilt of bananas. Journal of Fungi, 7(8). https://doi.org/10.3390/jof7080646
Hermanto, C., Sutanto, A., Jumjunidang, Edison, H. S., Daniells, J. W., Neill, W. T. O., Sinohin, V. G., Molina, A. B., & Taylor, P. (2011). Incidence and Distribution of Fusarium Wilt Disease of Banana in Indonesia. Acta Horticulturae, 897, 313–322. https://doi.org/10.17660/ActaHortic.2011.897.43
Hutabalian, M., Pinem, M., & Oemry, S. (2015). Uji Antagonisme Beberapa Jamur Saprofit Dan Endofit Dari Tanaman Pisang Terhadap Fusarium Oxysporum F.Sp. Cubens Di Laboratorium. Jurnal Online Agroekoteknologi, 3(2), 687–695.
Jayatri, H. N., Sumardiyono, C., & Wibowo, A. (2018). Race and Virulence Determination of Fusarium oxysporum f . sp . cubense Isolates from Sidomulyo Village of Bantul , Yogyakarta Penentuan Ras dan Virulensi Isolat Fusarium oxysporum f . sp . cubense Asal Desa Sidomulyo Kabupaten Bantul. Jurnal Perlindungan Tanaman Indonesia, 22(1), 72–81. https://doi.org/10.22146/jpti.26283
Jumjunidang, Edison, Riska, & Hermanto, C. (2012). Penyakit Layu Fusarium Pada Tanaman Pisang di Provinsi NAD: Sebaran dan Identifikasi Isolat Berdasarkan Analisis Vegetative Compatibility Group. Jurnal Hortikultura, 22(2), 164–171. https://doi.org/10.21082/jhort.v22n2.2012.p165-172
Kusuma, A. M., Rostaman, & Marsandi, K. (2020). Penyakit pada Tanaman Pisang dan Distribusinya di Wilayah Kecamatan Sumbang Kabupaten Banyumas. Jurnal Agro Wiralodra, 3(1), 8–15.
Li, C. Y., Mostert, G., Zuo, C. W., Beukes, I., Yang, Q. S., Sheng, O., Kuang, R. B., Wei, Y. R., Hu, C. H., Rose, L., Karangwa, P., Yang, J., Deng, G. M., Liu, S. W., Gao, J., Viljoen, A., & Yi, G. J. (2013). Diversity and Distribution of the Banana Wilt Pathogen Fusarium oxysporum F. Sp. cubense in China. Fungal Genomics & Biology, 3(2), 1–6. https://doi.org/10.4172/2165-8056.1000111
Mengesha, G. G., Yetayew, H. T., & Sako, A. K. (2018). Spatial distribution and association of banana (Musa spp.) Fusarium wilt (Fusarium oxysporum f.sp. cubense) epidemics with biophysical factors in southwestern Ethiopia. Archives of Phytopathology and Plant Protection, 51(11–12), 575–601. https://doi.org/10.1080/03235408.2018.1502067
Moore, N. Y., Hargreaves, P. A., Pegg, K. G., & Irwin, J. A. G. (1991). Characterisation of Strains of Fusarium oxysporum F.sp cubense. Aust. J. Bot, 39(1962), 161–166.
Nasir, N., Pittaway, P. A., Pegg, K. G., & Lisle, A. T. (1999). A pilot study investigating the complexity of Fusarium wilt of bananas in West Sumatra, Indonesia. Australian Journal of Agricultural Research, 50, 1279–1283.
Ordonez, N., Seidl, M. F., Waalwijk, C., Drenth, A., Kilian, A., Thomma, B. P. H. J., Ploetz, R. C., & Kema, G. H. J. (2015). Worse Comes to Worst: Bananas and Panama Disease—When Plant and Pathogen Clones Meet. PLoS Pathogens, 11(11), 1–7. https://doi.org/10.1371/journal.ppat.1005197
Pattikawa, A. S., Pagaya, J., & Patty, J. (2020). In vitro antagonism of the isolate mushroom Trichoderma harzianum to Fusarium oxysporum f.sp cubense causes wilt disease in banana kepok (Musa paradisiaca normalis). Rumphius Pattimura Biological Journal, 2(2), 50–55. https://doi.org/DOI https://doi.org/10.30598/rumphiusv2i2p050-055
Perrier, X., De Langhe, E., Donohue, M., Lentfer, C., Vrydaghs, L., Bakry, F., Carreel, F., Hippolyte, I., Horry, J. P., Jenny, C., Lebot, V., Risterucci, A. M., Tomekpe, K., Doutrelepont, H., Ball, T., Manwaring, J., De Maret, P., & Denham, T. (2011). Multidisciplinary perspectives on (Musa spp.) domestication. Proceedings of the National Academy of Sciences of the United States of America, 108(28), 11311–11318. https://doi.org/10.1073/pnas.1102001108
Pirenaningtyas, A., Muryani, E., & Santoso, D. H. (2020). Teknik Rekayasa Lereng untuk Pengelolaan Gerakan Massa Tanah di Dusun Bengle, Desa Dlepih, Kecamatan Tirtomoyo, Kabupaten Wonogiri, Provinsi Jawa Tengah. Jurnal Geografi?: Media Informasi Pengembangan Dan Profesi Kegeografian, 17(1), 15–22. https://doi.org/10.15294/jg.v17i1.21757
Rahayuniati, R. F., Hartono, S., Somowiyarjo, S., Subandiyah, S., & Thomas, J. E. (2021). Characterization of banana bunchy top virus on sumatra (Indonesia) wild banana. Biodiversitas, 22(3), 1243–1249. https://doi.org/10.13057/biodiv/d220321
Ritung, S., Nugroho, K., Mulyani, A., & Suryani, E. (2011). Petunjuk Teknis Evaluasi Lahan untuk Komoditas Pertanian (Edisi Revisi). In Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian, Badan Penelitian dan Pengembangan Pertanian.
Saptaningtyas, W. W. E., & Nurwidayati, T. (2020). Kajian Literatur Menuju Ekonomi Sirkular Untuk Pisang Dan Produk Olahannya. SNITT-Politeknik Negeri Balikpapan, 515–522.
Sari, W., & Irawan, I. (2018). Kelimpahan Dan Keragaman Bakteri Rizosfer Tanaman Pisang Serta Hubungannya Dengan Kejadian Penyakit Layu Fusarium. Agroscience (Agsci), 8(1), 54. https://doi.org/10.35194/agsci.v8i1.356
Selno, S., Zakiah, Z., & Kurniatuhadi, R. (2021). Kualitas Gaharu Aquilaria sp. dengan Pemberian Bioinokulan Fermentasi Batang Pisang yang Terkena Penyakit Layu Fusarium (Quality. Bios Logos, 11(2), 94–101. https://doi.org/https://doi.org/10.35799/jbl.11.2.2021.32551
Sitepu, F. E., Lisnawita, & Pinem, M. I. (2014). Penyakit Layu Fusarium (Fusarium oxysporum f. sp. cubense (E. F. Smith) Synd. & Hans.) pada Tanaman Pisang (Musa Spp.) dan Hubungannya dengan Keberadaan Nematoda Radopholus similis di Lapangan. Jurnal Online Agroekoteknologi, 2(2337), 1204–1211.
Teixeira, L., Heck, D., Nomura, E., Vieira, H., & Dita, M. (2021). Soil attributes, plant nutrition, and Fusarium wilt of banana in São Paulo, Brazil. Tropical Plant Pathology, 46(4), 443–454. https://doi.org/10.1007/s40858-021-00428-2
Thangavelu, R., Mostert, D., Gopi, M., Devi, P. G., Padmanaban, B., Molina, A. B., & Viljoen, A. (2019). First detection of Fusarium oxysporum f. sp. cubense tropical race 4 (TR4) on Cavendish banana in India. European Journal of Plant Pathology, 154(3), 777–786. https://doi.org/10.1007/s10658-019-01701-6
Triwidodo, H., Tondok, E. T., & Shiami, D. A. (2020). Pengaruh Varietas dan Umur Tanaman Berbeda terhadap Jumlah Populasi dan Tingkat Serangan Hama dan Penyakit Pisang (Musa sp.) di Kabupaten Sukabumi. Agrikultura, 31(2), 68. https://doi.org/10.24198/agrikultura.v31i2.27077
Widinugraheni, S., Niño-Sánchez, J., Van Der Does, H. C., Van Dam, P., García-Bastidas, F. A., Subandiyah, S., Meijer, H. J. G., Kistler, H. C., Kema, G. H. J., & Rep, M. (2018). A SIX1 homolog in Fusarium oxysporum f.sp. Cubense tropical race 4 contributes to virulence towards Cavendish banana. PLoS ONE, 13(10), 1–12. https://doi.org/10.1371/journal.pone.0205896
Zuo, C., Deng, G., Li, B., Huo, H., Li, C., Hu, C., Kuang, R., Yang, Q., Dong, T., Sheng, O., & Yi, G. (2018). Germplasm screening of Musa spp. For resistance to Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). European Journal of Plant Pathology, 151(3), 723–734. https://doi.org/10.1007/s10658-017-1406-3

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