Evolutionary tracks of chromosomal diversification in Trichopsis (Anabantiformes, Osphronemidae) fishes: New insights from a molecular cytogenetic perspective
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Mingkwan B, Sassi FDMC, Muanglenm N, Pinmongkhonkul S, Pinthong K, Tongnunui S, Yeesin P, Tanomtong A, Liehr T, Cioffi MDB, Supiwong W. 2023. Evolutionary tracks of chromosomal diversification in Trichopsis (Anabantiformes, Osphronemidae) fishes: New insights from a molecular cytogenetic perspective. Biodiversitas 24: 1551-1559. This study investigated the chromosomes of three Trichopsisspecies (namely Trichopsis pumila, Trichopsis vittata, and Trichopsis schalleri) using conventional (Giemsa stain, Ag-NOR) and molecularcytogenetic techniques. Fluorescence in situ hybridization (FISH) with repetitive DNAs, including 5S and 18S rDNAs, and microsatellites as probes were also performed. Our results indicated a conserved diploid number of 46 (2n) for all analyzed species of both sexes, although varying in their karyotype structure. Furthermore, T. schalleri and T. vittata karyotypes were presented with only acrocentric chromosomes (46a), while T. pumila had a submetacentric pair in addition to acrocentric ones (2sm+44a). Positive Ag-NOR sites were differentially found in each species adjacent to the acrocentric chromosome's centromeric and/or interstitial sub-centromeric regions. Both 5S and 18S rDNAs vary between the analyzed species, but all were localized in multiple sites. However, the distribution pattern of microsatellites differed in each species, mostly scattering at peri-centromeric and telomeric regions. These findings show that Trichopsis species, while having a conserved diploid number, differ significantly in the distribution of repetitive sequences in their karyotypes, particularly for rDNAs. These species-specific patterns can be useful for characterizing and identifying further different species and examining the evolutionary mechanisms that drove the evolution of these fishes' karyotypes.
##plugins.themes.bootstrap3.article.details##
References
Abu-Almaaty AH, Hassan MK, Bahgat IM, Suleiman MEE. 2017. Inter simple sequence repeat (ISSR) and cytogenetic analysis of three fish species of family osphronemidae. Egyptian Journal of Aquatic Biology and Fisheries 21(2): 1-15.
Arai R. 2011. Fish karyotypes: a check list. Springer, Tokyo, Japan 340 pp.
Baimai V. 2010. Biodiversity in Thailand. The Journal of the Royal Institute of Thailand 2: 107-118.
Chaiyasan P, Mingkwan B, Jantarat S, Suwannapoom C, Cioffi MB, Liehr T, Talumphai S, Tanomtong A, Supiwong W. 2021. Classical and molecular cytogenetics of Belontia hasselti (Perciformes: Osphronemidae): Insights into the ZZ/ZW sex chromosome system. Biodiversitas 22(2): 548-556.
Chaiyasan P, Supiwong W, Saenjundaeng P, Seetapan K, Pinmongkhonkul S, Tanomtong A. 2018. A report on classical cytogenetics of highfin Barb fish, Cyclocheilichthys armatus (Cypriniformes, Cyprinidae). Cytologia 83 (2): 149-154.
Chooseangjaew S, Tanyaros S, Maneechot N, Buasriyot P, Getlekha N, Tanomtong A. 2017. Chromosomal characteristics of the
tropical oyster, Crassostrea belcheri Sowerby, 1871 (Ostreoida, Ostreidae) by conventional and Ag-NOR banding
techniques. Cytologia 82: 3-8.
Cioffi MB, Bertollo LAC, Villa MA, Oliveira EA, Tanomtong A, Yano W, Supiwong W, Chaveerach A. 2015. Genomic organization of repetitive DNA elements and its implications for the chromosomal evolution of Channid fishes (Actinopterygii, Perciformes). PLoS One 10(6): e0130199.
Cioffi MB, Kejnovsky E, Bertollo LAC. 2011. The chromosomal distribution of microsatellite repeats in the genome of the wolffish Hoplias malabaricus, focusing on the sex chromosomes. Cytogenet Genome Res 132: 289-296.
Dernberg AF, Sedat JW, Hawley RS. 1996. Direct evidence of role for heterochromatin in meiotic chromosome segregation. Cell 86:135-146.
Donsakul T, Magtoon W, Rungsiruji A. 2009. Karyotypes of five belontiid fishes (Family Belongtiidae): Betta smaragdina, B. simplex, B. sp., Trichopsis pumila and T. Schalleri. The 35th Congress on Science and Technology of Thailand. Chonburi, Thailand. p. 77.
Ditcharoen S, Bertollo LAC, Rab P, Hnatkova, Molona WF, Liehr T, Tanomtomg A, Triantaphyllidis C, Ozouf-Costaz C, Tongnunui S, Pengseng P, Supiwong W, Aroutiounian R, Cioffi MB. 2019. Genomic organization of repetitive DNA elements and extensive karyotype diversity of Silurid Catfishes (Teleostei: Siluriformes): a comparative cytogenetic approach. International Journal of Molecular Sciences 20(3545): 1-18.
Ditcharoen S, Sassi FMC, Bertollo LAC, Molina WF, Liehr T. Saengjundaeng P, Tanomtong A, Supiwong W, Suwannapoom C, Cioffi MB. 2020. Comparative chromosomal mapping of microsatellite repeats reveals divergent patterns of accumulation in Siluridae (Teleostei: Siluriformes) species. Genetics and Molecular Biology 43(4): 1-11.
Esmaeili HR, Pirvar Z, Ebrahimi M, Matthias F. 2015. Karyological and molecular analysis of three endemic loaches (Actinopterygii: Cobitoidea) from Kor River basin, Iran. Molecular Biology Research Communications 4(1): 1-13.
Getlekha N, Cioffi MB, Yano CF, Maneechot N, Bertollo LAC, Supiwong W, Tanomtong A, Molina WF. 2016a. Chromosome mapping of repetitive DNAs in sergeant major fishes (Abudefdufinae, Pomacentridae): a general view on the chromosomal conservatism of the genus. Genetica 144: 567-576.
Getlekha N, Molina WF, Cioffi MB, Yano CF, Maneechot N, Bertollo LAC, Supiwong W, Tanomtong A. 2016b. Repetitive DNAs highlight the role of chromosome fusions in the karyotype evolution of Dascyllus species (Pomacentridae, Perciformes). Genetica 144(2): 203-211.
Getlekha N, Tanomtong A. 2020. First Cytogenetic Study of Green Chromis, Chromis viridis (Perciformes, Pomacentridae)
by Conventional Staining and Ag-NOR Banding Techniques. Chiang Mai University Journal of Natural Sciences 19(2): 164-175.
Gornung E. 2013. Twenty years of physical mapping of major ribosomal RNA genes across the Teleosts: A review of research. Cytogenetic and Genome Research 141(2-3): 90-102.
Juntaree S, Supiwong W. 2020. Standardized Karyotype and Idiogram of the Glass Goby Fish (Gobiopterus chuno) (Hamilton, 1822) in Thailand. Science Technology and Engineering Journal (STEJ) 6(2): 72-81.
Jantarat S, Supiwong W, Phintong K, Sonsrin K, Kong-ngarm N, Tanomtong A. 2017. First Analysis on the Cytogenetics of Painted Sweetlip, Plectorhinchus pictus (Heamulidae: Perciformes) from Thailand. Cytologia 82(2): 145-150.
Kasiroek W, Indananda C, Pinthong K, Supiwong W, Pengseng P, Tanomtong A. 2017. NOR Polymorphism and Chromosome Analysis of Banggai Cardinalfish, Pterapogon kauderni (Perciformes, Apogonidae). Cytologia 82(1): 17-23.
Khakhong S, Supiwong W, Tanomtong A, Sriuttha M, Jearranaiprepame P, Soemphol W, Jiwyam W. 2014. A First Chromosomal
Characterization of NORs in Splendid Snakehead Fish, Channa lucius (Perciformes, Channidae). Cytologia 79(2): 133-
Kottelat M. 2001. Fishes of Laos. WHT Publications Ltd., Colombo 5, Sri Lanka, 198 p.
Kumar R, Kushwaha B, Nagpure NS, Behera BK, Lakra WS. 2013. Karyological and molecular diversity in three freshwater
species of the genus Channa (Teleostei, Perciformes) from India. Caryologia: International Journal of Cytology,
Cytosystematics and Cytogenetics 66(2): 109-119.
Kumar R, Sahoo PK, Vishwanath W, Barat A. 2014. Karyotype of a new loach schistura obliquofascia and a mahseer Puntius
chelynoides from Kumaun Hills of Himalaya. Cytologia 79(2): 243-246.
Levan A, Fredga K, Sandberg A. A. 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52: 201-220.
Magtoon W, Rangsiruji A, Donsakul T. 2007. Karyotypes of Betta splendens, B. prima, Trichopsis vittatus and
Trichogaster trichopterus (family Belontiidae) from Thailand. The 33rd Congress on Science and Technology of
Thailand, Nakhon Sri Thammarat, Thailand. p. 94.
Maneechot N, Yano CF, Bertollo LAC, Getlekha N, Molina WF, Ditcharoen S, Tengjaroenkul B, Supiwong S, Tanomtong A,
Cioffi MB. 2016. Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias
(Clariidae, Siluriformes). Molecular Cytogenetics 9(4): 1-10.
Mingkwan B, Chaiyachate, Tanomtong A, Supiwong S. 2021. First Karyotypic Study of the Kissing Gourami, Helostoma
temminckii (Cuvier, 1829) (Anabantiformes, Helostomatidae) from Thailand by Conventional and Ag-NOR Staining Techniques. Science Technology and Engineering Journal (STEJ) 7(2): 9-22.
Moraes RLR, Bertollo LAC, Marin MMF, Yano CF, Hatanaka T, Barby FF, Troy WP, Cioffi MB. 2017. Evolutionary relationships and cytotaxonomy considerations in the genus Pyrrhulina (Characiformes Lebiasinidae). Zebrafish 14: 536-546.
Nelson JS, Grande, TC, Wilson MVH. 2016. Fishes of the World: Fifth Edition. In Fishes of the World; Fifth Edition.
Pasa R, Kavalco KF, Penteado P, Kavalco SAF, Toledo LT. 2009. Gene mapping of 18S and 5S rDNA genes in the karyotype of
the three-spot Gourami Trichogaster trichopterus (Perciformes, Osphronemidae). Zebrafish 6(3):219-222.
Pereira CS, Pazian MF, Rab P, Collares-Pereira MJ. 2015. Dynamics of Rex3 in the genomes of endangered lberian Leuciscinae
(Cyprinidae) and their natural hybrids. Mol Cytogenet 8(81).
Phimphan S, Tanomtong A, Jumrusthanasan S, Supiwong W, Siripiyasing P, Sanoamuang L. 2013. First Report of NOR
Polymorphism and Chromosome Analysis of John’s Snapper, Lutjanus johnii (Perciformes, Lutjanidae) in Thailand.
Cytologia 78(4): 335-344.
Pinthong K, Supiwong W, Simporn B, Chooseangjaew S, Kakampuy W, Tanomtong A. 2017. A first chromosomal and nucleolar
organizer regions (NORs) analyses of Cheveys sheetfish, Micronema cheveyi (Siluriformes, Siluridae). Cytologia 82(4):
- 441.
Pissaparn M, Phimphan S, Chaiyasan P, Tanomtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First
chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21(9): 4309-4316.
Rebordinos L, Cross I, Merlo A. 2013. High evolutionary dynamism in 5S rDNA of fish: Strate of the Art. Cytogenet Genome Res
: 103-13.
Sangpakdee W, Phimphan S, Tanomtong A, Patawang I, Pinthong K, Neeratanaphan L. 2015. Karyological study of Lutjanus
ehrenbergii and L. carponotatus (perciformes, lutjanidae) by classical and Ag-NOR staining techniques. Nucleus (India) 58(3): 1-7.
Sangpakdee W, Phimphan S, Tengjaroenkul B, Pinthong K, Neeratanaphan L, Tanomtong A. 2017. Cytogenetic study of three
microhylid species (Anura, Microhylidae) from Thailand. Cytologia 82: 67-74.
Singhato A, Judprasong K, Sridonpai P, Laitip N, Ornthai N, Yafa C, Chimkerd C. 2022. Effect of Different Cooking Methods on
Selenium Content of Fish Commonly Consumed in Thailand. Foods 11: 1808.
Sochorová J, Garcia S, Gálvez F, Symonová R, Kova?í A. 2018. Evolutionary trends in animal ribosomal DNA loci: Introduction to a new online database. Chromosoma 127(1): 141-150.
Sreeputhorn K, Mangumphan K, Muanphet B, Tanomtong A, Supiwong W, Kaewmad P. 2017. The First Report on Chromosome Analysis of F1 Hybrid Catfish: Mekong Giant Catfish (Pangasianodon gigas)×Striped Catfish (Pangasianodon hypophthalmus) and Spot Pangasius (Pangasius larnaudii)×Pangasianodon hypophthalmus (Siluriformes, Pangasiidae). Cytologia 82(4):457-463.
Supiwong W, Liehr T, Cioffi MB, Chaveerach A, Kosyakova N, Pinthong K, Tanee T, Tanomtong A. 2014. Chromosomal evolution in naked catfishes (Bagidae, Siluriformes): A comparative chromosome mapping study. Zool Anz 253(4): 316-320.
Supiwong W, Jiwyam W, Sreeputhorn K, Maneechot N, Bertollo LAC, Cioffi MB, Getlekha N, Tanomtong A. 2017a. First report
on classical and molecular cytogenetics of archerfish, Toxotes chatareus (Perciformes: Toxotidae). The nucleus 60(3):
-359.
Supiwong W, Phimphan S, Kaewmad P, Saenjundaeng P, Jantarat S, Tanomtong A. 2017b. First Cytogenetic Study of the Whitecheek Monocle Bream, Scolopsis vosmeri (Perciformes, Nemipteridae) from Thailand. Cytologia 82(5): 481-484.
Supiwong W, Wongchantra P, Thongnetr W, Mingkwan B, Chaiyasan P, Pinmongkhonkul S, Pinthong K, Tanomtomg A. 2021. Comparative cytogenetic analysis of fishes in the genus Trichopodus (Osphronemidae) in Thailand. Biodiversitas 22(7): 3029-3036.
Singh M, Kumar R, Nagpure NS, Kushwaha B, Mani I, Murmu K, Chauhan UK, Lakra WS. 2010. Nucleotide sequences and chromosomal localization of 45S and 5S rDNA in Neolissochilus hexagonolepis (Pisces, Cyprinidae), using dual-color fish. Zoolog Sci 27(8): 709-716.
Symonova R, Majtanova Z, Sember A, Staaks GBO, Bohlen J, Freyhof J. Genome differentiation in a species pair of coregoninefishes: An extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplication. BMC Evol. Biol 13: 42-52.
Taki Y, Ohtsuka R, Komodo M, Natori Y, Utsugi K, Shibukawa K, Oizumi T, Ottomanski S, Praxaysombath B, Phongsa K, Magtoon W, Musikasinthorn P, Grudpan C, Grudpan J, Suvarnaraksha A, So N, Thach P, Penh P, Nguyen PT, Tran DD, Tran LX. 2021. Fishes of the Indochinese Mekong, Tokyo, Japan 545pp.
Tanomtong A, Supiwong W, Jearranaiprepame P, Khakhong S, Kongpironchuen C, Getlekha N. 2014. A new natural autotetraploid
and chromosomal characteristics of dwarf snakehead fish, Channa gachua (Perciformes, Channidae) in Thailand.
Cytologia 79(1): 15-27.
Yeesin P, Buasriyot P, Ditcharoen S, Chaiyasan P, Suwannapoom C, Juntaree S, Jantarat S, Talumphai S, Cioffi MB, Liehr T,
Tanomtong A, Supiwong W. 2021. Comparative study of four Mystus species (Bagridae, Siluriformes) from Thailand:
insights into their karyotypic diversity. Comparative Cytogenetics 15(2):119-136.
Vidthayanon C. 2005. Freshwater Fish in Thailand. 5th ed. Nanmeebook Press, Bangkok.
Zworykin DD. 2016. Phylogenesis of reproductive strategies in labyrinth fishes (Anabantoidei) and their sisterly groups. Zhurnal
Obshchei Biologii 77(6): 464-881.
Most read articles by the same author(s)
- PATCHARAPORN CHAIYASAN, BOONYADA MINGKWAN, SITTHISAK JANTARAT, CHATMONGKON SUWANNAPOOM, MARCELO DE BELLO CIOFFI, THOMAS LIEHR, SUCHEELA TALUMPHAI, ALONGKLOD TANOMTONG, WEERAYUTH SUPIWONG, Classical and molecular cytogenetics of Belontia hasselti (Perciformes: Osphronemidae): Insights into the ZZ/ZW sex chromosome system , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 2 (2021)
- NATTASUDA DONBUNDIT, PHICHAYA BAUSRIYOD, ALONGKLOD TANOMTONG, NATTAPONG SRISAMOOT, MONTRI SUMONTHA, WEERA THONGNETR, ISARA PATAWANG, WEERAYUTH SUPIWONG, SUKHONTHIP DITCHAROEN, NAWARAT MUANGLEN, PUNTIVAR KAEWMAD, Karyomorphological delineation, and the NOR loci on the sex chromosome in three species of Chrysopeleinid (Chrysopeleinae: Colubridae) from Thailand , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 7 (2022)
- WEERAYUTH SUPIWONG, PRAYOON WONGCHANTRA, WEERA THONGNETR, BOONYADA MINGKWAN, PATCHARAPORN CHAIYASAN, SITTHISAK PINMONGKHONKUL, KRIT PINTHONG, ALONGKLOD TANOMTONG, Comparative cytogenetic analysis of fishes in the genus Trichopodus (Osphronemidae) in Thailand , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 7 (2021)
- MANACHAYA PISSAPARN, SUMALEE PHIMPHAN, PATCHARAPORN CHAIYASAN, ALONGKLOD TANOAMTONG, THOMAS LIEHR, CHATMONGKON SUWANNAPOOM, MONTRI REUNGSING, WEERAYUTH SUPIWONG, First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866) , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 9 (2020)