Karyomorphological delineation, and the NOR loci on the sex chromosome in three species of Chrysopeleinid (Chrysopeleinae: Colubridae) from Thailand

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DOI https://doi.org/10.13057/biodiv/d230758
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Vol. 23 No. 7 (2022)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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NATTASUDA DONBUNDIT
Biology Program, Faculty of Science, Khon Kaen University. Munag, Khon Kaen 44000, Thailand
PHICHAYA BAUSRIYOD
Biology Program, Faculty of Science, Khon Kaen University. Munag, Khon Kaen 44000, Thailand
ALONGKLOD TANOMTONG
Biology Program, Faculty of Science, Khon Kaen University. Munag, Khon Kaen 44000, Thailand
NATTAPONG SRISAMOOT
Division of Biotechnology, Faculty of Agricultural Technology, Kalasin University. Muang, Kalasin 46000, Thailand
MONTRI SUMONTHA
Ranong Marine Fisheries Research and Development Station. 157 Chaloem Phrakiat Rd., Paknam, Muang, Ranong 85000, Thailand
WEERA THONGNETR
Walai Rukhavej Botanical Research Institute, Mahasarakham University. Kantharawichai, Maha Sarakham, Thailand
ISARA PATAWANG
Department of Biology, Faculty of Science, Chiang Mai University. Muang, Chiang Mai, Thailand
WEERAYUTH SUPIWONG
Faculty of Interdisciplinary Studies, Nong Khai Campus, Khon Kaen University. Muang, Nong Khai 43000, Thailand
SUKHONTHIP DITCHAROEN
Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi. Khlong 6, Khlong Luang, Pathum Thani 12110, Thailand
NAWARAT MUANGLEN
Department of Fisheries, Faculty of Agricultural Technology, Sakon Nakhon Rajabhat University. Sakon Nakhon 47000, Thailand
PUNTIVAR KAEWMAD ♥
Major of Biology, Faculty of Science and Technology, Mahasarakham Rajabhat University. Muang, Maha Sarakham 44000, Thailand

Abstract


Abstract. Donbundit N, Bausriyod P, Tanomtong A, Srisamoot N, Sumontha M, Thongnetr W, Patawang I, Supiwong W, Ditcharoen S, Muanglen N, Kaewmad P. 2022. Karyomorphological delineation, and the NOR loci on the sex chromosome in three species of Chrysopeleinid (Chrysopeleinae: Colubridae) from Thailand. Biodiversitas 23: 3813-3819. The description of chromosomal characteristics of three snakes from Thailand namely, Ahaetulla fusca, A. prasina, and Chrysopelea ornata was by using conventional staining and Ag-NOR banding techniques. The results showed that the same diploid chromosome number (2n) = 36 (16 macrochromosomes + 20 microchromosomes) and the fundamental number (NF) was 54 while the karyotype formulae in these three species are as follows: A. fusca; 2n(36) = Lm6 + Sm2+ Ssm4 + Sa2+ ZZ/ZW + 20 microchromosomes, A. prasina; 2n(36) = Lm6+ Sm6+ Sa2+ ZZ/ZW + 20 microchromosomes and C. ornata; 2n(36) = Lm4+ Lsm2+ Sm2 + Ssm4+ Sa2+ ZZ/ZW + 20 microchromosomes. Besides, the ZZ/ZW sex chromosome was observed in all species, whereas C. ornata was discovered the heteromorphic sex chromosome for the first time. Nucleolar organizer regions (NORs) are located on telomeric region of one pair in all species, while located on microchromosomes in A. fusca and A. prasina, whereas C. ornata was detected on sex chromosomes. Therefore, the Z chromosome revealed the NOR on the telomeric region of the long arm, while NOR of the W chromosome was located on the telomeric region of the short arm.


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