Performance of cultured marine sponges-symbiotic bacteria as a heavy metal bio-adsorption




Abstract. Marzuki I, Ahmad R, Kamaruddin M, Asaf R, Armus R, Siswanty I. 2021. Performance of cultured marine sponges-symbiotic bacteria as a heavy metal bio-adsorption. Biodiversitas 22: 5536-5543. One source of heavy metal waste could be coming from industrial disposal. Contamination and accumulation of hazardous heavy metal waste are most likely to occur in water areas, It has become a global issue that urgently requires appropriate technology to address. Two marine sponge-symbiotic bacteria from the Spermonde islands, Indonesia, i.e., Bacillus cohnii strain DSM 6307 (BS) and Pseudomonas stutzeri RCH2 (PS), were used in this study. The purpose of this study is to investigate the mechanism of heavy metal bio-adsorption of two sponge symbiotic bacteria by contact technique and are assessed their activity, capacity, and efficiency of bio-adsorption against different heavy metals (Cr, Mn, Fe, Co, Cu, Zn, Ag, and Cd),. The bio-adsorption capacity was evaluated by Atomic Absorption Spectroscopy (AAS) after a predetermined contact time (4, 8, 12, and 16 days). The results demonstrated that two sponge symbiotic bacteria had bio-adsorption activities against eight different heavy metals. BS bacteria exhibited higher bio-adsorption capacity (Cr ? Zn ? Cu ? Fe ? Co ?Mn and Ag ? Cd) than that of PS bacteria (Zn ? Co ? Fe ? Mn ? Cu ? Cr and Cd ? Ag). The difference in the atomic number of heavy metals causes the characteristics of these metals to vary which affects the capacity and efficiency of bio-adsorption of sponge symbiotic bacteria to heavy metals.


Alimardan M, Ziarati P, Moghadami RJ. 2016. Adsorption of Heavy Metal Ions from Contaminated Soil by B. integerrima Barberry. Biomedical & Pharmacology Journal 9(1):1-8,
Chávez GMDCA, González CR. 2013. Tolerance of Chrysantemum maximum to heavy metals: the potential for its use in the revegetation of tailings heaps. Journal Environ Sci. 25:367-375,
Gebregewergis A. 2020. Levels of selected metals in white teff grain samples collected from there different areas of Ethiopia by using Microwave Plasma Atomic Emission Spectroscopy (MP-AES). International Journal of Novel Research in Physics Chemistry & Mathematics, 7(1), 13–24.
Gjorgieva D, Kadifkova-Panovska T, Ba?eva K, Stafilov T. 2011. Assessment of heavy metal pollution in Republic of Macedonia using a plant assay. Arch Environ Contam Toxicol. 60:233–240,
Gupta AK, Verma SK, Khan K, Verma RK. 2013. Phytoremediation using aromatic plants: a sustainable approach for remediation of heavy metals polluted sites. Environ Sci Technol., 47:10115–10116,
Han Y, Chen G, Chen Y, Shen Z. 2015. Cadmium toxicity and alleviating effects of exogenous salicylic acid in Iris hexagona. Bull Environ. Contam Toxicol. 95:796–802,
Kamaruddin M, Tokoro M, Moshiur, Rahman M, Arayama S, Hidayati APN, Syafruddin D., … Kawahara E. 2014. Molecular characterization of various trichomonad species isolated from humans and related mammals in Indonesia. Korean Journal of Parasitology, 52(5), 471–478,
Kamaruddin M, Triananinsi N, Sampara N, Sumarni-, Minarti, RAAM. 2020. Karakterisasi DNA Mikrobiota Usus Bayi pada Persalinan Normal yang diberi ASI dan Susu Formula. Media Kesehatan Masyarakat, 16(1), 116–126.,
Karimpour M, Ashrafi SD, Taghavi K, Mojtahedi A, Roohbakhsh E, & Naghipur D. 2018. Adsorption of cadmium and lead onto live and dead cell mass of Pseudomonas aeruginosa?: A dataset. Elsevier, 18, 1185–1192.
Konkolewska A, Piechalak A, Ciszewska L, Antos-krzemi N, & Skrzypczak T. 2020. Combined use of companion planting and PGPR for the assisted phytoextraction of trace metals ( Zn , Pb , Cd ). Environmental Science and Pollution Research, (Februari),
Lajayer BA, Moghadam NK, Maghsoodi MR, Ghorbanpour M, Kariman K. 2019. Phytoextraction of heavy metals from contaminated soil, water and atmosphere using ornamental plants: mechanisms and efficiency improvement strategies, Environmental Science and Pollution Research, Springer Nature,
Liu YF, Mbadinga SM, Gu DJ, Mu BZ. 2017. Type II chaperonin gene as a complementary barcode for 16S rRNA gene in study of Archaea diversity of petroleum reservoirs, Int. J. Biodeterior & Biodegradation, 123 (December):113–120,
Marzuki I, Alwi RS, Erniati, Kamaruddin M, Sinardi, Iryani AS. 2019b. Chitosan Performance Of Shrimp Shells In The Biosorption Ion Metal Of Cadmium, Lead And Nickel Based On Variations pH Interaction, 1st Int. Conf. Materials Engineering and Management Section (ICMEM). Atlantic Press, Advances in Engineering Research,165: 6-11,
Marzuki I, Iksan MA, Marzuki AA, Angela A. 2019a. Aplication of Sea Sponge Micro Symbiont As A New Biomaterial To Reduce Chromium Heavy Metal Toxicity. Journal Al-Kimia, 7(1): 67-75, [in Indonesia]
Marzuki I, Sinardi S, Pratama I, Chaerul M, Paserangi I, Mudyawati M, & Asaf R. 2021a. Performance of sea sponges micro symbionts as a biomaterial in biodegradation naphthalene waste of modified. In The 5th International Seminar on Sustainable Urban Development; IOP Conference Series: Earth and Environmental Science Vol. 737, (p. 012016).
Marzuki I, Ali MY, Syarif HU, Erniati, Gusty S, Ritnawati, … Nisaa K. 2021c. Investigation of Biodegradable Bacteria as Bio indicators of the Presence of PAHs Contaminants in Marine Waters in the Marine Tourism Area of Makassar City. In The 6th International Conference on Tropical and Coastal Region Eco (p. 012006).
Marzuki I, Daris L, Nisaa K, & Emelda A. 2020b. The power of biodegradation and bio-adsorption of bacteria symbiont sponges sea on waste contaminated of polycyclic aromatic hydrocarbons and heavy metals. In IOP Conference Series: Earth and Environmental Science Vol. 584, (p. 012013).
Marzuki I, Daris L, Yunus S, & Riana AD. 2020c. Selection and characterization of potential bacteria for polycyclic aromatic biodegradation of hydrocarbons in sea sponges from Spermonde Islands , Indonesia. AACL Bioflux, 13(6), 3493–3506.
Marzuki I, Kamaruddin M, & Ahmad R. 2021b. Identification of marine sponges-symbiotic bacteria and their application in degrading polycyclic aromatic hydrocarbons. Journal Biodiversitas, 22(3), 1481–1488.
Marzuki I, Pratama I, Ismail HE, Paserangi I, Kamaruddin M, Chaerul M, & Ahmad R. 2021d. The Identification and Distribution Components of Polycyclic Aromatic Hydrocarbon Contaminants at the Port of Paotere , Makassar , South Sulawesi. In The 1st International Conference on Biotechnology and Food Sciences IOP Conf. Series: Earth and Environmental Science IOP Publishing Vol. 679, (p. 012017).
Marzuki I. 2020a. The Bio-adsorption Pattern Bacteria Symbiont Sponge Marine Against Contaminants Chromium and Manganese In The Waste Modification of Laboratory Scale. Indonesia Chimica Acta, 13(1), 1–9.
Melawaty L, Noor A, Harlim T, Nicole de V. 2014. Essential Metal Zn in Sponge Callyspongia aerizusa from Spermonde Archipelago, Advances in Biological Chemistry Journal 4: 86-90, 10.4236/abc,2014.41012
Minarti, Triananinsi N, Sampara N, Sumarni & Kamaruddin M. 2020. Metagenomic Diversity of Gut Microbiota of Gestational Diabetes Mellitus of Pregnant Women. Jurnal Biomedika, 13(01), 1–8.
Motaghi M, & Ziarati P. 2016. Adsorptive removal of cadmium and lead from Oryza sativa rice by banana peel as bio-sorbent. Biomedical and Pharmacology Journal, 9(2), 739–749.
Muszy?ska E, Labudda M, Kami?ska I, Górecka M, & Bederska-B?aszczyk M. 2019. Evaluation of heavy metal-induced responses in Silene vulgaris ecotypes. Protoplasma, (May).
Orania AM, Baratsa A, Vassilevab E, Thomasc OP. 2018. Marine sponges as a powerful tool for trace elements biomonitoring studies in coastal environment, Marine Pollution Bulletin 131: 633–645,
Pawar PR, and Al-Tawaha A.RMS. 2017. Marine sponges as Bioindicator species of Environmental Stress at Uran (Navi Mumbai), west coast of India, Am. J. Sustain. Agric., 11 (3): 29–37
Seo SB, Kajiuchi T, Kim DI, Lee SH, Kim HK. 2012. Preparation of Water Soluble Chitosan Blendmers and Their Application to Removal of Heavy Metal Ions from Wastewater. J. Macromol. Res. 10(2): 103–107,
Shama SA, Moustafa ME, Gad MA. 2010. Removal of heavy metals Fe3+, Cu2+, Zn2+, Pb2+, Cr3+ and Cd2+ From Aquaeus Solutins by Using Eichornia Crassipes, Journal Electrochimica Acta 28 (2):125-133,
Siahaya N, Noor A, Heyrani NS, Nicole de V. 2014. A preliminary Effort to assign Sponge (Callyspongia sp) as trace metal biomonitor for Pb, Cd, Zn, and Cr, an enviromental persfective in hative gulf water Ambon, Advances in Biological Chemistry Journal 3: 549-552, abc, 2013.26062
Sobrinho NMB, do Amaral De AAFM, Abreu LES, & Zonta E. 2020. Metals Phytoextraction by Cordia africana from Soils Contaminated with Oil Drilling Waste. Floresta e.Ambiente, 27(1), 1–8,
Tam N, Wong Y. 2008. Effectiveness of bacterial inoculum and mangrove plants on remediation of sediment contaminated with polycyclic aromatic hydrocarbons, Marine Pollution Bulletin 57: 716-728,
Wibowo N, Nurcahyo R, Gabriel DS. 2019. Sponge Iron Plant Feasibility Studi In Kalimantan, Indonesia. ARPN Journal of Engineering and Applied Sciences 14(23): 4013-4020
Zhang S, Zhou Y, Nie W, Song L, Zhang T. 2012. Preparation of Uniform Magnetic Chitosan Microcapsules and Their Application in Adsorbing Copper Ion (II) and Chromium Ion (III). Indonesian Eng. Chemistry Res. 51(1): 14098–14106,
Zhou Y, Xia S, Zhang Z, Zhang J, Hermanowicz SW. 2011. Associated Adsorption Characteristics of Pb ( II ) and Zn ( II ) by a Novel Biosorbent Extracted from Waste-Activated Sludge. J. Environ. Engineering II: 1–7
Ziarati P, Moshiri IM, Sadeghi P. 2017. Bio-adsorption of Heavy Metals from Aqueous Solutions by Natural and Modified non-living Roots of Wild Scorzonera incisa DC. Sci Discov. 1 (99): 1–8.