A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia
Article Sidebar
Abstract Views: 320
File Views: 61
Main Article Content
Abstract
Abstract. Sutami, Purwanto, Rosariastuti R. 2021. A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia. Biodiversitas 22: 3785-3791. Isolation and characterization of indigenous bacteria from the soil of traditional gold mining contaminated with mercury is the first step in a series of research to explore and utilize indigenous bacteria in Jendi's area. This study was aimed to determine the characteristics and identity of bacterial isolates from soil of traditional gold mining in Jendi Village, Wonogiri contaminated by mercury. The methods used in this study included bacterial isolation, media preparation, phenotypic identification including; morphological and physiological tests and genotyping tests. The results showed that the bacterial isolate A3 grew optimally in media with the addition of 10% NaCl, at a temperature of 27°C, and pH 9. There were negative reactions to the observations of gram staining, acid production from glucose, indole production, catalase and urease, and positive reactions to oxidation. A neighbor-joining phylogenetic tree based on the 16S rRNA gene sequence showed that the A3 strain was closely related to Sphingosinicella microcystinivorans strain Y2T (JCM 13185T) with 100% Query coverage and a maximum identity of 99.56%.
Article Details
Issue
Section
References
Aanniz T, Ouadghiri M, Melloul M, Swings J, Elfahime E, Ibijbijen J, Ismaili M, Amar M. 2015. Thermophilic bacteria in moroccan hot springs, salt marshes and desert soils. Brazilian Journal of Microbiology, 46(2), 443–453. DOI: 10.1590/S1517-838246220140219
Alpers CN, Hunerlach MP, May JT, Hothem RL, Taylor HE, Antweiler RC, De Wild JF, Lawler DA. 2005. Geochemical characterization of water, sediment, and biota affected by mercury contamination and acidic drainage from historical gold mining, Greenhorn Creek, Nevada County, California, 1999–2001: U.S. Geological Survey Scientific Investigations Report 2004-5251, 278 p. http://pubs.usgs.gov/sir/2004/5251/
Aygan A, Arikan B. 2007. Mini review an overview on bacterial motility detection. International Journal of Agri?culture and Bi?ology, 9(1), 193–196. http://www.fspublishers.org
Brink B. 2010. Urease Test Protocol. American Society for Microbiology,11: 1–10. http://www.microbelibrary.org/
Buzzini P, Turchetti B, Yurkov A. 2018. Extremophilic yeasts: the toughest Extremophilic yeasts: the toughest yeasts around? Yeast, 35(8), 487–497. DOI: 10.1002/yea.3314
Dewi NR, Setiani O, Gold B, Village J, Subdistrict S, District W. 2013. Hubungan riwayat paparan merkuri dengan gangguan keseimbangan tubuh pada penambang emas tradisional di Desa Jendi Kecamatan Selogiri Kabupaten Wonogiri. Jurnal Kesehatan Lingkungan Indonesia, 12(1), 70–74. DOI: 10.14710/jkli.12.1.70-74
Dutu R. 2015. Making the most of natural resources in indonesia. OECD Economic Survey of Indonesia, 97–134. DOI: 10.1787/eco_surveys-idn-2015-6-en
Fan L, Song J, Hildebrand PD, Forney CF. 2002. Interaction of ozone and negative air ions to control micro-organisms. Journal of Applied Microbiology, 93 (1), 144–148. DOI: 10.1046/j.1365-2672.2002.01683.x
Geueke B, Fleischmann T, Ka P, Kohler HE. 2007. Description of Sphingosinicella xenopeptidilytica sp. nov., a b -peptide-degrading species, and emended descriptions of the genus Sphingosinicella and the species Sphingosinicella microcystinivorans. 107–113. DOI: 10.1099/ijs.0.64509-0
Gondal AH, Qammar F, Sidra S, Shamal SK, Muhammad DT, Asma Z, Bushara R. 2021. Adaptability of soil ph through innovative microbial approach. Current Research in Agricultural Sciences. 8(2): 71-79. DOI: 10.18488/journal.68.2021.82.71.79
Hii YS, Yen San C, Lau SW, Danquah MK. 2020. Isolation and characterisation of phosphate solubilizing microorganisms from peat. Biocatalysis and Agricultural Biotechnology, 26(5), 101643. DOI: 10.1016/j.bcab.2020.101643
Ji B, Zhu L, Wang S, Liu Y. 2021. Temperature-effect on the performance of non-aerated microalgal-bacterial granular sludge process in municipal wastewater treatment. Journal of Environmental Management, 282(11), 111955. DOI: 10.1016/j.jenvman.2021.111955
Khanifah K, Udin U, Hadi N, Alfiana F. 2020. Environmental performance and firm value: Testing the role of firm reputation in emerging countries. International Journal of Energy Economics and Policy, 10(1), 96–103. DOI: 10.32479/ijeep.8490
Li J, Miao X, Hao Y, Xie Z, Zou S, Zhou C. 2020. Health risk assessment of metals (Cu, Pb, Zn, Cr, Cd, As, Hg, Se) in angling fish with different lengths collected from Liuzhou, China. International Journal of Environmental Research and Public Health, 17(7), 1–16. DOI: 10.3390/ijerph17072192
Lionel. 2016. Why Is Catalase So Fast? A Preliminary Net-work Hypothesis for the Rapid Enzyme-cata-lysed Decomposition of Hydrogen Peroxide. Water. 7, 129–146. DOI: 10.14294/WATER.2016.1
Ludwig W, Euzéby J, Whitman WB. 2010. Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. In Bergey’s Manual® of Systematic Bacteriology. DOI: 10.1007/978-0-387-68572-4_2
Ma’arif MR. 2014. Gerakan perlawanan lsm cagar tuban terhadap pembangunan PT. HOLCIM INDONESIA. Paradigma, 2(1), 1–9. https://jurnalmahasiswa.unesa.ac.id/index.php/paradigma/article/view/6639
MacWilliams MP. 2009. Indole Test Protocol. American Journal for Microbiology, December 2009, 1–9. https://asm.org/getattachment/200d3f34-c75e-4072-a7e6-df912c792f62/indole-test-protocol-3202.pdf
Maruyama T, Park H, Ozawa K, Tanaka Y, Sumino T, Hamana K, Hiraishi A, Kato K. 2006. Sphingosinicella microcystinivorans gen. nov., sp. nov., a microcystin-degrading bacterium. International Journal of Systematic and Evolutionary Microbiology, 85–89. DOI:10.1099/ijs.0.63789-0
Miyata M, Robinson R C, Uyeda TQP, Fukumori Y, Fukushima S, ichi, Haruta S, Homma M, Inaba K, Ito M, Kaito C, Kato K, Kenri T, Kinosita Y, Kojima S, Minamino T, Mori H, Nakamura S, Nakane D, Nakayama K,Wakabayashi K. 2020. Tree of motility – A proposed history of motility systems in the tree of life. Genes to Cells, 25(1), 6–21. DOI: 10.1111/gtc.12737
Moreno M de L, Pérez D, García MT, Mellado E. 2013. Halophilic bacteria as a source of novel hydrolytic enzymes. Life, 3(1), 38–51. DOI: 10.3390/life3010038
Oren A. 2008. Microbial life at high salt concentrations: Phylogenetic and metabolic diversity. Saline Systems, 4(1), 1–13. DOI: 10.1186/1746-1448-4-2
Public Health England. 2018. Motility test. UK Standards for Microbiology Investigations. 21(4). https://www.gov.uk/uk-standards-for-microbiologyinvestigations-smi-quality-and-consistency-in-clinical-laboratories
Public Health England. 2019. Oxidation / Fermentation of Glucose Test. UK Standards for Microbiology Investigations, TP 27(4), 1–13. 4. https://www.gov.uk/uk-standards-formicrobiology-investigations-smi-quality-and-consistency-in-clinical-laboratories
Qiao Z, Cao M, Wang D, Liao S, Wang G. 2007. Sphingosinicella humi sp. nov., isolated from arsenic- contaminated farmland soil and emended description of the genus Sphingosinicella. International Journal of Systematic and Evolutionary Microbiology, January, 0–6. DOI: 10.1099/ijsem.0.003186
Reiner K. 2010. Catalase-test-protocol. American Society for Microbiology, 1–9. https://www.asmscience.org/content/education/protocol/protocol.3226
Rhani HC. 2012. Faktor-faktor yang berhubungan dengan kadar merkuri (Hg) pada tanah pemukiman warga di sekitar lokasi penambangan emas tradisional (Studi Kasus di Desa Jendi, Kecamatan Selogiri, Kabupaten Wonogiri, Provinsi Jawa Tengah). Jurnal Kesehatan Masyarakat Universitas Diponegoro, 1(2), 18738. http://ejournals1.undip.ac.id/index.php/jkm
Selid PD, Xu H, Collins EM, Face-Collins MS, Zhao JX. 2009. Sensing mercury for biomedical and environmental monitoring. Sensors, 9(7), 5446–5459. DOI: 10.3390/s90705446
Shields P,Cathcart L. 2016. Oxidase test protocol. ASM Conference for Undergraduate Educators, November 2010, 1–5. https://asm.org/getattachment/00ce8639-8e76-4acb-8591-0f7b22a347c6/oxidase-test-protocol-3229.pdf
Stackebrandt E, Goebel BM. 1994. Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic Bacteriology, 44(4), 846–849. DOI: 10.1099/00207713-44-4-846
Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. 2012. Molecular, clinical and environmental toxicicology Volume 3: Environmental Toxicology. In Molecular, Clinical and Environmental Toxicology (Vol. 101). DOI: 10.1007/978-3-7643-8340-4
Vadde. 2020. Flagellar motility in bacteria structure and function of flagellar motor. Journal of Pharmaceutical Sciences and Research, 12(4), 558–560. DOI: 10.1016/S1937-6448(08)01402-0
Waheed R, El Asely AM, Bakery H, El-Shawarby R, Abuo-Salem M, Abdel-Aleem N, Malhat F, Khafaga A, Abdeen A. 2020. Thermal stress accelerates mercury chloride toxicity in Oreochromis niloticus via up-regulation of mercury bioaccumulation and HSP70 mRNA expression. Science of the Total Environment, 718, 137326. DOI: 10.1016/j.scitotenv.2020.137326
Wang C, He S, Zou Y, Liu J, Zhao R, Yin X, Zhang H, Li Y. 2020. Quantitative evaluation of in-situ bioremediation of compound pollution of oil and heavy metal in sediments from the Bohai Sea, China. Marine Pollution Bulletin, 150(June), 110787. DOI: 10.1016/j.marpolbul.2019.110787
Winardi W, Haryono E, Sudrajat S, Soetarto ES. 2020. In situ bioremediation strategies for the recovery of mercury-contaminated land in abandoned traditional gold mines in Indonesia. Biosaintifika, 12(3), 469–477. DOI: 10.15294/biosaintifika.v12i3.25229
Yasir M, Aslam Z, Song GC, Jeon CO, Chung YR. 2010. Sphingosinicella vermicomposti sp. nov., isolated from vermicompost, and emended description of the genus Sphingosinicella. International Journal of Systematic and Evolutionary Microbiology, 60(3), 580–584. DOI: 10.1099/ijs.0.010777-0
Yoon JH, Kang SJ, Lee JS, Nam SW, Kim W, Oh TK. 2008. Sphingosinicella soli sp. nov., isolated from an alkaline soil in Korea. International Journal of Systematic and Evolutionary Microbiology, 58(1), 173–177. DOI: 10.1099/ijs.0.65242-0
Zulaikhah ST, Wahyuwibowo J, Pratama AA. 2020. Mercury and its effect on human health: A review of the literature. International Journal of Public Health Science, 9(2), 103–114. DOI: 10.11591/ijphs.v9i2.20416