Toxicity of entomopathogenic fungal culture filtrate of lowland and highland soil of South Sumatra (Indonesia) against Spodoptera litura larvae




Abstract. Gustianingtyas M, Herlinda S, Suwandi, Suparman, Hamidson H, Hasbi, Setiawan A, Verawaty M, Elfita, Arsi. 2020. Toxicity of entomopathogenic fungal culture filtrate of lowland and highland soil of South Sumatra against Spodoptera litura larvae. Biodiversitas 21: 1839-1849. The use of secondary fungal metabolites for the active ingredient of mycoinsecticide is more effective and more easily integrated with other pest control techniques. This study aimed to measure the toxicity of the culture filtrate of entomopathogenic fungi originating from South Sumatra against the Spodoptera litura larvae. Beauveria bassiana (25 isolates) and Metarhizium anisopliae (20 isolates) of South Sumatra were cultured in liquid media and filtered to produce culture filtrate. The larvae which were sick due to the filtrate showed the symptoms of decreased appetite and were not actively moving, while the dead larvae were characterized by being wrinkled, dry, black integument, and odorless. Mortality caused by B. bassiana filtrate was the highest 98% (BJgTs isolate) and not significantly different from the BSwTd2 isolate (94.67%). Yet, the LT50 BSwTd2 isolate was shorter (5.92 days) compared to the LT50 BJgTs isolate (6.35 days). The most toxic M. anisopliae filtrate produced the mortality of 96% (MKbTp2 isolate) and 85.33% (MPdB isolate) each of which had LT50 of 7.36 days and 8.09 days, respectively. So, the most toxic culture filtrate was BSwTd2 isolate of B. bassiana and MKbTp2 isolate of M. anisopliae. The entomopathogenic fungi producing filtrate which are toxic have the potential to be active ingredients of mycoinsecticides.


Altre JA, Vandenberg JD. 2001. Factors influencing the infectivity of isolates of Paecilomyces fumosoroseus against diamondback moth, Plutella xylostella. J Invertebr Pathol 78: 31-36. DOI: 10.1006/jipa.2001.5043
Augustyniuk-Kram A, Kram KJ. 2012. Entomopathogenic fungi as an important natural regulator of insect outbreaks in forests review. Forest Ecosystem, More than Just Trees. Blanco JA. (ed.). In Tech: Croatia. DOI: 10.5772/30596
Ayudya D, Herlinda S, Suwandi S. 2019. Insecticidal activity of culture filtrates from liquid medium of Beauveria bassiana isolates from South Sumatra (Indonesia) wetland soil against larvae of Spodoptera litura. Biodiversitas 20: 2101–2109. DOI: 10.13057/biodiv/d200802
Bandani AR, Khambay BPS, Faull L, Newton R, Deadman M, Butt TM. 2000. Production of efrapeptins by Tolypocladium species and evaluation of their insecticidal and antimicrobial properties. Mycol Research 104: 537-544. DOI: 10.1017/S0953756299001859
Darlan N, Pradiko I, Winarna, Siregar HH. 2016. Effect of El Niño 2015 on oil palm performance in central and Southern Sumatera. J Tanah dan Iklim 40: 113–120. [Indonesian]. DOI: 10.2017/jti.v40n2.2016.113-120
El-Ghany TMA. 2015. Entomopathogenic Fungi and their Role in Biological Control. Biology Department Faculty of Science Jazan University KSA. Cairo. DOI:10.4172/978-1-63278-065-2-66
El-Husseini MMM. 2019. Efficacy of the entomopathogenic fungus, Metarhizium anisopliae (Metsch.), against larvae of the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), under laboratory conditions. Egypt J Biol Pest Control 29: 4–6. DOI: 10.1186/s41938-019-0156-2
Fattah A, Syam S, Daud ID, Dewi VS, Rahman A. 2018. The intensity of leaf damage caused by attack of Spodoptera litura F and seed yield on some soybean varieties in South Sulawesi Indonesia. Scientific Research J 6: 55–60.
Fernandes EKK, Rangel DEN, Moraes AM, Bittencourt VREP, Roberts DW. 2007. Variability in tolerance to UV-B radiation among Beauveria spp. isolates. J Invertebr Pathol 96: 237?243. DOI: 10.1016/j.jip.2007.05.007
Garrido-Jurado J, Torrent J, Barrón V, Corpas A, Quesada-Moraga E. 2011. Soil properties affect the availability, movement, and virulence of entomopathogenic fungi conidia against puparia of Ceratitis capitata (Diptera: Tephritidae). Biol Control 58: 277-285. DOI: 10.1016/j.biocontrol.2011.05.017
Herlinda S, Yudha S, Thalib R, Khodijah, Suwandi, Lakitan B, Verawaty M. 2018a. Species richness and abundance of spiders inhabiting rice in fresh swamps and tidal lowlands in South Sumatera, Indonesia. J ISSAAS 24: 82-93.
Herlinda S, Rizkie L, Suwandi, Susilawati, Lakitan B, Verawaty M, Hasbi. 2018b. Effects of high temperature and ultraviolet-C irradiance on conidial viability and density of Beauveria bassiana and Metarhizium anisopliae isolated from soils of lowland ecosystems in Indonesia. Eurasian J Anal Chem 13: 209–216.
Johari A, Herlinda S, Pujiastuti Y, Irsan C, Sartiami, D. 2016. Morphological and genetic variation of Thrips parvispinus (Thysanoptera: Thripidae) in chili plantation (Capsicum annuum L.) in the lowland and highland of Jambi Province, Indonesia. American J Bio Sci 2: 17–21. DOI: 10.11648/j.ajbio.s.2014020601.14
Juhriah, Azrai M, Tambaru E, Rahayu JE. 2019. Phenotypic characteristics and grouping of hybrid waxy corn Zea mays L. J Ilmu Alam dan Lingkungan 10: 51–60. [Indonesian]. DOI: 10.20956/jal.v10i1.6624
Karenina T, Herlinda S, Irsan C, Pujiastuti Y. 2019. Abundance and species diversity of predatory arthropods inhabiting rice of refuge habitats and synthetic insecticide application in freshwater swamps in South Sumatra, Indonesia. Biodiversitas 20 (8): 2375-2387. DOI:
Kartika K, Lakitan B, Wijaya A, Kadir S, Widuri L.I, Siaga E. 2018. Effects of particle size and application rate of rice-husk biochar on chemical properties of tropical wetland soil, rice growth and yield. Australian J Crop Sci 12: 817–826. DOI: 10.21475/ajcs.18.12.05.PNE1043
Khachatourians GG, Qazi SS, Reid WR. 2007. Biochemistry and molecular biology of entomopathogenic fungi. In: Brakhage AA, Zipfer PF (eds.). The Mycota VI: Human and animal relationships. Springer-Verlag, Berlin.
Liu SF, Wang GJ, Nong XQ, Liu B, Wang M, Li SL, Cao GC, Zhang ZH. 2017. Entomopathogen Metarhizium anisopliae promotes the early development of Peanut Root. Plant Protect Sci 53: 101–107. DOI: 10.17221/49/2016-PPS
Luo Z, Ren H, Mousa JJ, Rangel DEN, Zhang Y, Bruner SD, Keyhani NO. 2017. The PacC transcription factor regulates secondary metabolite production and stress response, but has only minor effects on virulence in the insect pathogenic fungus Beauveria bassiana. Environ Microbiol 19: 788–802. DOI: 10.1111/1462-2920.13648
Mancillas-Paredes JM, Hernández-Sánchez H, Jaramillo-Flores ME, García-Gutiérrez C. 2019. Proteases and chitinases induced in Beauveria bassiana during infection by Zabrotes subfasciatus. Southwestern Entomologist 44: 125-137. DOI: 10.3958/059.044.0114
Maqsood S, Sabri, MA, Ali A, Abbas M, Aziz A. 2017. Comparative toxicity of some insecticides against army worm, Spodoptera litura L. (Lepidoptera: Noctuidae) under laboratory conditions. J Entomology and Zoology Studies 5: 770–773.
Maryanto M, Sukiyono K, Priyono B. 2018. Technical efficiency analysis and determinants in potato farming (Solanum tuberosum L.) in Pagar Alam City, South Sumatra Province. Agraris 4: 1–8. [Indonesian]. DOI: 10.18196/agr.4154
Mascarin GM, Jackson MA, Behle RW, Kobori NN, Júnior ÍD. 2016. Improved shelf life of dried Beauveria bassiana blastospores using convective drying and active packaging processes. Appl Microbiol Biotechnol 100: 8359–8370. DOI: 10.1007/s00253-016-7597-2
Mascarin GM, Jackson MA, Kobori NN, Behle RW, Delalibera Júnior Í. 2015. Liquid culture fermentation for rapid production of desiccation tolerant blastospores of Beauveria bassiana and Isaria fumosorosea strains. J Invertebr Pathol 127: 11–20. DOI: 10.1016/j.jip.2014.12.001
Munir J, Herman W. 2019.Various physical and soil chemical properties phenomena support the food security in West Sumatera. Ziraa’ah 44: 146–153. [Indonesian]. DOI: 10.31602/zmip.v44i2.1736
Nagal G. Verma K, Rathore L. 2016. Management of Spodoptera litura (Fabricius) through some novel insecticides and biopesticides on bell pepper under polyhouse environment. Advances in Life Sci 5: 1081–1084.
Nasution K, Respatijarti. 2019. Yield trial on eight potential lines F7 generation of chili pepper (Capsicum Annuum L.) compact type in Low Land. J Produksi Tanaman 7: 464–473. [Indonesian]
Oreste M, Baser N, Bubici G, Tarasco E. 2015. Effect of Beauveria bassiana strains on the Ceratitis capitata - Psyttalia concolor system. Bulletin Insectology 68: 265–272.
Peña-Peña AJ, Santillán-Galicia MT, Hernández-López J, Guzmán-Franco AW. 2015. Metarhizium pingshaense applied as a seed treatment induces fungal infection in larvae of the white grub Anomala cincta. J Invertebrate Pathology 130: 9–12. DOI: 10.1016/j.jip.2015.06.010
Pinnamaneni R., Kalidas P, Rao KRSS. 2010. Cloning and Expression of Bbchit1 gene of Beauveria bassiana. Open Entomology J 4: 30–35. DOI: 10.2174/1874407901004010030
Prabawati G, Herlinda S, Pujiastuti Y. 2019. The abundance of canopy arthropods in South Sumatra (Indonesia) freshwater swamp main and ratooned rice applied with bioinsecticides and synthetic insecticide. Biodiversitas 20: 2921–2930. DOI: 10.13057/biodiv/d201021
Safitri A, Herlinda S, Setiawan, A. 2018. Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas 19: 2365–2373. DOI: 10.13057/biodiv/d190647
Sagrim M, Sumule AI, Iyai DA, Baransano M. 2017. Prime potency of agriculture commodities on highland of Arfak Mountains Regency, Papua Barat. J Ilmu Pertanian Indonesia 22: 141–146. [Indonesian]. DOI: 10.18343/jipi.22.3.141
Situmorang S, Haryono D, Soelaiman A. 2019. Analysis of business performance and welfare of vegetable farmers in Pagar Alam City South Sumatera Province. Indonesian J Socio Economics 1: 84–92. [Indonesian]
Soesanto L, Mugiastuti E, Manan A. 2019. Raw secondary metabolites application of two Trichoderma harzianum isolates towards vascular streak dieback on cocoa seedlings. Pelita Perkebunan 35: 22–32. DOI: 10.22302/iccri.jur.pelitaperkebunan.v35i1.346
Sumikarsih E, Herlinda S. Pujiastuti Y. 2019. Conidial density and viability of Beauveria bassiana isolates from java and Sumatra and their virulence against Nilaparvata lugens at different temperatures. Agrivita J Agric Sci 1: 335–349.
Turnip A, Runtuboi DYP, Lantang D. 2018. Effectivity test of Beauveria bassiana fungus and application time of Spodoptera litura in mustard greens (Brassica juncea). J Biologi Papua, 10: 26–31. [Indonesian]. DOI: 10.31957/jbp.131
Zamhari A, Sitorus S, Pravitasari A. 2017. Analysis of the competitive commodities and its direction of development plan in Pagar Alam City, South Sumatera Province. Tata Loka, 19: 218–229. [Indonesian]. DOI: 10.14710/tataloka.19.3.218-229
Zibaee A, Bandani AR, Talaei-Hassanlouei R, Malagoli D. 2011. Cellular Immune Reactions of the Sunn Pest, Eurygaster integriceps, to the Entomopathogenic Fungus, Beauveria bassiana and Its Secondary Metabolites. J Insect Sci 11: 1–16. DOI: 10.1673/031.011.13801
Zibaee A, Bandani AR, Tork M. 2009. Effect of the entomopathogenic fungus, Beauveria bassiana, and its secondary metabolite on detoxifying enzyme activities and acetylcholinesterase (AChE) of the Sunn pest, Eurygaster integriceps (Heteroptera: Scutellaridae). Biocontrol Sci Technol 19: 485–498. DOI: 10.1080/09583150902847127

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