Identification of bacteria in Rhizophora mucronata leaf litter and sediment
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Abstract. Yulma, Kustanti A, Soemarno, Mahmudi M, Ihsan B. 2025. Identification of bacteria in Rhizophora mucronata leaf litter and sediment. Biodiversitas 26: 3054-3060. The mangrove ecosystem is an area that has high productivity, with litter production that contributes to increasing nutrients. This study aimed to determine the bacterial community in mangrove vegetation, especially in sediment and decomposed Rhizophora mucronata leaf litter. This study was conducted in the Mangrove and Crab Conservation Area. R. mucronata leaf litter was collected with a 2 × 2 m container. Furthermore, the leaf litter was dried at 105°C until its weight was constant. The decomposition rate and bacterial identification were measured at intervals of 14, 28, 42 and 56 days. Meanwhile, sediment was taken at different depths, namely 10, 20, and 30 cm, and as much as 250 g for each depth using a biopore drill. Identification of bacteria in litter and sediment was carried out with three tests, namely: Gram staining test (Gram-negative, Gram-positive and bacterial forms), primary test (3% KOH, 3% H2O2 and Oxidase) and further test (O/f, Glucose and motility). The results showed that the bacterial community that plays a role in decomposing organic matter and accelerating the rate of decomposition in R. mucronata leaf litter is diverse with different characteristics, including Bacillus sp., Aeromonas sp., Pseudomonas sp., Listeria sp., Actinobacillus sp., Micrococcus sp., and Acinetobacter sp. At the same time, the bacterial community found in the sediment includes Pseudomonas sp., Aeromonas sp., Micrococcus sp., Bacillus sp., Flavobacterium sp., Enterobacteria sp., and Listeria sp. The most dominant bacteria found is Bacillus sp. because one of the endophytic bacteria found in plant tissue can increase plant growth through mechanisms such as biological nitrogen fixation, phosphate and potassium solubilization.
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References
Alam MI, Debrot AO, Ahmed MU, Ahsan MN, Verdegem MC. 2021. Synergistic effects of mangrove leaf litter and supplemental feed on water quality, growth and survival of shrimp (Penaeus monodon, Fabricius, 1798) post larvae. Aquaculture 545: 737237. DOI: 10.1016/j.aquaculture.2021.737237.
Arfarita N, Nuraini Y, Rinady MVP, Noerhayati E, Prayogo C. 2024. The impact of different management on pine-based agroforestry system and litter accumulation on the population and activity of cellulolytic bacteria. Biodiversitas 25: 924-936 DOI: 10.13057/biodiv/d250303.
Chan K-Y, Wong SH, Mak CY. 1979. Effects of bottom sediments on the survival of Enterobacter aerogenes in seawater. Mar Pollut Bull 10 (7): 205-210. DOI: 10.1016/0025-326X(79)90534-4.
Chen J, Chen G, Gu Y, Zhu H, Ye Y. 2020. Fate of leaf litter in restored Kandelia obovata (S. L.) mangrove forests with different ages in Jiulong River Estuary, China. Restor Ecol 28 (2): 369-377. DOI: 10.1111/rec.13079.
Chollet S, Maillard M, Schörghuber J, Grayston SJ, Martin J-L. 2021. Deer slow down litter decomposition by reducing litter quality in a temperate forest. Ecology 102 (2): e03235. DOI: 10.1002/ecy.3235.
Clores MA, Conde MAA, Perez JDV. 2020. Biomass leaching and dynamics of nutrients, microbial abundance and activity during decomposition of seagrass Cymodocea rotundata necromass. Appl Environ Res 42 (1): 1-13. DOI: 10.35762/aer.2020.42.1.1.
Cristiano G, Di Sabatino A. 2023. How does water current velocity affect invertebrate community and leaf-litter breakdown in a physicochemically stable freshwater ecosystem? An experimental study in twonearby reaches (erosional vs depositional) of the Vera Spring (Central Italy). Ecohydrology 17 (4): e2532. DOI: 10.1002/eco.2532.
Du N, Li W, Qiu L, Zhang Y, Wei X, Zhang X. 2020. Mass loss and nutrient release during the decomposition of sixteen types of plant litter with contrasting quality under three precipitation regimes. Ecol Evol 10 (7): 3367-3382. DOI: 10.1002/ece3.6129.
Farid SM, Gobel SA. 2023. Analysis of mangrove forest litter productivity in Tutuwoto Village, Anggrek District, North Gorontalo Regency. Jambura Edu Biosfer J 5 (2): 36-42. DOI: 10.34312/jebj.v5i2.22012. [Indonesian]
Gao X, Zheng Z, Diao Z, Zhang Y, Wang Y, Ma L. 2024. The effects of litter input and increased precipitation on soil microbial communities in a temperate grassland. Front Microbiol 15: 1347016. DOI: 10.3389/fmicb.2024.1347016.
Godson PS, Vincent SGT, Krishnakumar S. 2022. Ecology and Biodiversity of Benthos. Elsevier Inc, Amsterdam, Netherlands.
Goodman ED, Johnston-Peck AC, Dietze EM, Wrasman CJ, Hoffman AS, Abild-Pedersen F, Bare SR, Plessow PN, Cargnello M. 2019. Catalyst deactivation via decomposition into single atoms and the role of metal loading. Nat Catal 2 (9): 748-755. DOI: 10.1038/s41929-019-0328-1.
Guo Y, Ren C, Yi J, Doughty R, Zhao F. 2020. Contrasting responses of rhizosphere bacteria, fungi and arbuscular mycorrhizal fungi along an elevational gradient in a temperate montane forest of China. Front Microbiol 11: 2042. DOI: 10.3389/fmicb.2020.02042.
Kaurichev IS, Shishova VS. 1967. Oxidation reduction conditions of coarse textured soils of the Meschera lowland. Sov Soil Sci 5: 636-646.
Khomutovska N, Jasser I, Isidorov VA. 2024. Unraveling the role of bacteria in nitrogen cycling: Insights from leaf litter decomposition in the Knyszyn Forest. Forests 15 (6): 1065. DOI: 10.3390/f15061065.
Li H, Chen C, Zhou J, Bai H, Zhang S, Liu Q. 2024. Exotic mangrove Laguncularia racemosa litter input accelerates nutrient cycling in mangrove ecosystems. Front Plant Sci 15: 1463548. DOI: 10.3389/fpls.2024.1463548.
Li Q, Moorhead DL, DeForest JL, Henderson R, Chen J, Jensen R. 2009. Mixed litter decomposition in a managed Missouri Ozark forest ecosystem. For Ecol Manag 257: 688-694. DOI: 10.1016/j.foreco.2008.09.043.
Liu J, Ding C, Zhang W, Wei Y, Zhou Y, Zhu W. 2022. Litter mixing promoted decomposition rate through increasing diversities of phyllosphere microbial communities. Front Microbiol 13: 1009091. DOI: 10.3389/fmicb.2022.1009091.
Liu X, Huang Z, Havrilla CA, Liu Y, Wu G-L. 2021. Plant litter crust role in nutrients cycling potentials by bacterial communities in a sandy land ecosystem. Land Degrad Dev 32 (11): 3194-3203. DOI: 10.1002/ldr.3973.
Lu K, Yang Q, Jiang Y, Liu W. 2022. Changes in temporal dynamics and factors influencing the environment of the bacterial community in mangrove rhizosphere sediments in Hainan. Sustainability 14 (12): 7415. DOI: 10.3390/su14127415.
Lu X, Gu X, Zhang L, Zhang J, Wang W, Wang M. 2024. Tidal zone effects on the diet composition of leaf-eating crabs in natural mangrove communities: A stable isotope analysis. Front Mar Sci 11: 1351183. DOI: 10.3389/fmars.2024.1351183.
Maeda HA. 2016. Lignin biosynthesis: Tyrosine shortcut in grasses. Nat Plants 2 (6): 16080. DOI: 10.1038/nplants.2016.80.
Marathe SK, Vashistht MA, Prashanth A, Parveen N, Chakraborty S, Nair SS. 2018. Isolation, partial purification, biochemical characterization and detergent compatibility of alkaline protease produced by Bacillus subtilis, Alcaligenes faecalis and Pseudomonas aeruginosa obtained from sea water samples. J Genet Eng Biotechnol 16 (1): 39-46. DOI: 10.1016/j.jgeb.2017.10.001.
Marin MAL, Strejcek M, Junkova P, Suman J, Santrucek J, Uhlik O. 2021. Exploring the potential of Micrococcus luteus culture supernatant with resuscitation-promoting factor for enhancing the culturability of soil bacteria. Front Microbiol 12: 685263. DOI: 10.3389/fmicb.2021.685263.
Masagca JT. 2024. Feeding ecology of tree-climbing mangrove sesarmid crabs from Luzon, Philippines. Biotropia 16 (1): 1-10. DOI: 10.11598/btb.2009.16.1.61.
McBride MJ. 2014. The family Flavobacteriaceae. In: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F (eds). The Prokaryotes. Springer, Berlin, Heidelberg. DOI: 10.1007/978-3-642-38954-2_130.
Mugivhisa LL, Manganyi MC. 2025. Green catalysis: The role of medicinal plants as food waste decomposition enhancers/accelerators. Life 15 (4): 552. DOI: 10.3390/life15040552.
Muwawa EM, Obieze CC, Makonde HM, Jefwa JM, Kahindi JHP, Khasa DP. 2021. 16S rRNA gene amplicon-based metagenomic analysis of bacterial communities in the rhizospheres of selected mangrove species from Mida Creek and Gazi Bay, Kenya. PLoS One 16 (3): e0248485. DOI: 10.1371/journal.pone.0248485.
Nuñez M. 2014. Micrococcus. In: Encyclopedia of Food Microbiology, Volume 2. Elsevier, New York. DOI: 10.1016/b978-0-12-384730-0.00206-8.
Oliveira JL, Xin H, Chai L, Millman ST. 2019. Effects of litter floor access and inclusion of experienced hens in aviary housing on floor eggs, litter condition, air quality, and hen welfare. Poult Sci 98 (4): 1664-1677. DOI: 10.3382/ps/pey525.
Pepper IL, Gerba CP, Gentry TJ. 2015. Environmental Microbiology Third Edition. Elsevier Inc, New York.
Pereira APA, Durrer A, Gumiere T, Gonçalves JLM, Robin A, Bouillet J-P, Wang J, Verma JP, Singh BK, Cardoso EJBN. 2019. Mixed Eucalyptus plantations induce changes in microbial communities and increase biological functions in the soil and litter layers. For Ecol Manag 433: 332-342. DOI: 10.1016/j.foreco.2018.11.018.
Perangin-angin NSB, Adriman A, El Fajri N. 2014. The Production and Decomposition Rate of Mangrove Litter in The Sungai Alam Village, Bengkalis Sub-district, Bengkalis Regency, Riau Province. [Doctoral Dissertation]. Universitas Riau, Riau. [Indonesian]
Purahong W, Wubet T, Lentendu G, Schloter M, Pecyna MJ, Kapturska D, Hofrichter M, Krüger D, Buscot F. 2016. Life in leaf litter: Novel insights into community dynamics of bacteria and fungi during litter decomposition. Mol Ecol 25: 4059-4074. DOI: 10.1111/mec.13739.
Rannavre R, Donde S. 2023. Mini Review: Some notes on the impact of Millipedes (Myriapoda: Diplopoda) on fungi and bacteria. J Bacteriol Mycol 10 (3): 1211. DOI: 10.26420/jbacteriolmycol.2023.1211.
Santoyo G, Moreno-Hagelsieb G, del Carmen Orozco-Mosqueda M, Glick BR. 2016. Plant growth-promoting bacterial endophytes. Microbiol Res 183: 92-99. DOI: 10.1016/j.micres.2015.11.008.
Sarker S, Masud?Ul?Alam M, Hossain MS, Chowdhury SR, Sharifuzzaman SM. 2021. A review of bioturbation and sediment organic geochemistry in mangroves. Geological J 56 (5): 2439-2450. DOI: 10.1002/gj.3808.
Wang Z, Feng J-X, Li X-P, Zhang J. 2021. Whole-genome sequencing of Micrococcus luteus MT1691313, isolated from the Mariana Trench. Microbiol Resour Announc 10: e0036921. DOI: 10.1128/mra.00369-21.
Yulma Y, Ihsan B, Awaludin, Zainuddin, Bija S, Rani M, Andira A, Ramadani F, Sunarti. 2020. Identification of bacteria from mangrove forest in Mamburungan, Tarakan City. IOP Conf Ser: Earth Environ Sci 564: 012020. DOI: 10.1088/1755-1315/564/1/012020.
Yulma Y, Ihsan B, Sunarti S, Malasari E, Wahyuni N, Mursyban M. 2017. Identification of bacteria in decomposed mangrove leaf litter in the mangrove and proboscis monkey conservation area (KKMB) in Tarakan City. J Trop Biodivers Biotechnol 2: 28-33. DOI: 10.22146/jtbb.27173. [Indonesian]
Yulma Y, Wijayanti T. 2024. Total nitrogen content in sediments in the mangrove and crab conservation area of Tarakan City. Jurnal Perikanan Unram 14 (3): 1448-1456. DOI: 10.29303/jp.v14i3.983.
Yunasfi, Susetya IE, Utomo B, Dalimunthe A, Samsuri, Zaitunah A. 2024. Fungal diversity associated with the decomposition of Avicennia marina leaf litter at various salinity levels. Biodiversitas 25 (2): 792-800. DOI: 10.13057/biodiv/d250239.
Zhang C, Bijl E, Svensson B, Hettinga K. 2019a. The extracellular protease AprX from Pseudomonas and its spoilage potential for UHT milk: A review. Compr Rev Food Sci Food Saf 18 (4): 834-852. DOI: 10.1111/1541-4337.12452.
Zhang Y, Yu X, Zhang W, Lang D, Zhang X, Cui G, Zhang X. 2019b. Interactions between endophytes and plants: Beneficial effect of endophytes to ameliorate biotic and abiotic stresses in plants. J Plant Biol 62: 1-13. DOI: 10.1007/s12374-018-0274-5
Zhang Y, Zhang Q, Yang W, Zhang Y, Wang N, Fan P, You C, Yu L, Gao Q, Wang H, Zheng P, Wang R. 2023. Response mechanisms of 3 typical plants nitrogen and phosphorus nutrient cycling to nitrogen deposition in temperate meadow grasslands. Front Plant Sci 14: 1140080. DOI: 10.3389/fpls.2023.1140080.
Zhang C, de Pasquale S, Hartman K, Stanley CE, Berendsen RL, Van der Heijden MGA. 2024. The microbial contribution to litter decomposition and plant growth. Environ Microbiol Rep 16 (1): e13205. DOI: 10.1111/1758-2229.13205.
Zhou X, Chen X, Qi X, Zeng Y, Guo X, Zhuang G, Ma A. 2023. Soil bacterial communities associated with multi-nutrient cycling under long-term warming in the alpine meadow. Front Microbiol 14: 1136187. DOI: 10.3389/fmicb.2023.1136187.