Morphology and phytochemical potential of Vaccinium varingiifolium in Mount Lawu, Karanganyar District, Central Java, Indonesia
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Abstract
Abstract. Roziaty E, Santhyami, Agustina P, Aryani I, Fathin MA, Rahmania SA, Sholihin MYN, Salsabilla DF. 2025. Morphology and phytochemical potential of Vaccinium varingiifolium in Mount Lawu, Karanganyar District, Central Java, Indonesia. Biodiversitas 26: 3209-3217. Vaccinium varingiifolium, commonly known as Cantigi, is a subalpine shrub endemic to high-altitude ecosystems above 2,000 meters. Mount Lawu, located in Central Java, Indonesia, represents one of its key habitats. Despite its ecological relevance, the species remains underexplored, particularly in terms of its morphological and phytochemical traits. This study aimed to characterize the morphological features and phytochemical composition of V. varingiifolium to understand its adaptive strategies and ecological role better. A combination of qualitative and quantitative exploratory approaches was employed, using purposive sampling. Morphological assessments, including microscopic examinations, were conducted on stems, leaves, flowers, and fruits. Phytochemical screening using spectrophotometry targeted secondary metabolites, while ecological observations assessed environmental factors influencing adaptation. Morphological characterization of V. varingiifolium in Lawu Mountains revealed that young leaves are purplish-red, turning green with increased tissue density upon maturation. Stems are brown, globular, and lignified. Flowers are purplish-red, bell-shaped (campanulate), and axillary in position. Fruits are oval (5-6 mm in diameter), transitioning from green to black as they ripen. Phytochemical analyses revealed the presence of flavonoids and tannins, compounds known for their antioxidant, antibacterial, anti-inflammatory, and wound-healing properties. The plant thrives at elevations of 2,900-3,100 meters, where higher temperatures facilitate rapid regeneration. These findings provide important insights into the morphological adaptations and chemical defenses of V. varingiifolium, supporting conservation initiatives and enriching our understanding of plant survival mechanisms in subalpine environments, particularly in the context of climate change.
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References
Argent G, Wilkie P. 2020. Six new species of Vaccinium (Ericaceae) from New Guinea. Edinburgh J Bot 77 (3): 439-453. DOI: 10.1017/S0960428620000104.
Argent G. 2019. Rigiolepis and Vaccinium (Ericaceae) in Borneo. Edinburgh J Bot 76 (1): 55-172. DOI: 10.1017/S0960428618000276.
Arivo D, Mubarik NR, Rusmana I, Batubara I. 2023. Antioxidant activity of various solvent extracts from endophytic bacteria isolated from girang (Leea indica) leaves. Biodiversitas 24 (1): 415-422. DOI: 10.13057/biodiv/d240148.
Barcelo R. 2015. Phytochemical screening and antioxidant activity of edible wild fruits in Benguet, Cordillera Administrative Region, Philippines. Electron J Biol 11 (3): 80-89.
Blume CL. 1825. Bijdragen tot de flora van Nederlandsch Indië (Vol. 1). Ter Lands Drukkerij, Batavia. [Dutch]
Casolo V, Braidot E, Petrussa E, Zancani M, Vianello A, Boscutti F. 2020. Relationships between population traits, nonstructural carbohydrates, and elevation in alpine stands of Vaccinium myrtillus. Am J Bot 107 (4): 639-649. DOI: 10.1002/ajb2.1458.
Cavieres LA, Hernández-Fuentes C, Sierra-Almeida A, Kikvidze Z. 2016. Facilitation among plants as an insurance policy for diversity in Alpine communities. Funct Ecol 30 (1): 52-59. DOI: 10.1111/1365-2435.12545.
Cetinkaya H, Kulak M, Karaman M, Karaman HS, Kocer F. 2017. Flavonoid accumulation behavior in response to the abiotic stress: Can a uniform mechanism be illustrated for all plants? In: Justino GC (eds). Flavonoids-From Biosynthesis to Human Health. IntechOpen, London. DOI: 10.5772/68093.
Cronin DT, Libalah MB, Bergl RA, Hearn GW. 2014. Biodiversity and conservation of tropical montane ecosystems in the Gulf of Guinea, West Africa. Arct Antarct Alp Res 46 (4): 891-904. DOI: 10.1657/1938-4246-46.4.891.
Devi AM, Devi KK, Devi PP, Devi ML, Das S. 2023. Metabolic engineering of plant secondary metabolites: Prospects and its technological challenges. Front Plant Sci 14: 1171154. DOI: 10.3389/fpls.2023.1171154.
Djamas N, Pancoro A, Devy L. 2024. The effect of drought stress on phyllanthin and quercetin contents of green meniran plant (Phyllanthus niruri L.). Jurnal Hortikultura Indonesia 15 (2): 112-118. DOI: 10.29244/jhi.15.2.112-118.
Elferjane MR, Milutinovi? V, Krivoku?a MJ, Taherzadeh MJ, Pietrzak W, Marinkovi? A, Jovanovi? AA. 2024. Vaccinium myrtillus L. leaf waste as a source of biologically potent compounds: Optimization of polyphenol extractions, chemical profile, and biological properties of the extracts. Pharmaceutics 16 (6): 740. DOI: 10.3390/pharmaceutics16060740.
Forney CF, Javorek SK, Jordan MA, Kloet SPV. 2012. Floral volatile composition of four species of Vaccinium. Botany 90 (5): 365-371. DOI: 10.1139/B2012-008.
Frazier AG, Brewington L. 2020. Current changes in Alpine ecosystems of Pacific Islands. In: Goldstein MI, DellaSala DA (eds). Encyclopedia of the World's Biomes. Elsevier Inc, Amsterdam, Netherlands. DOI: 10.1016/b978-0-12-409548-9.11881-0.
Geng Y, Zhang Y, Ranjitkar S, Huai H, Wang Y. 2016. Traditional knowledge and its transmission of wild edibles used by the Naxi in Baidi Village, Northwest Yunnan province. J Ethnobiol Ethnomed 12: 10. DOI: 10.1186/s13002-016-0082-2.
Hu J, Wang J, Li S, Yang B, Gong M, Li X, Zhang L, Tian J. 2016. Phytochemical compositions, antioxidant and antimicrobial activities analysis of extracts from Vaccinium bracteatum Thunb. leaves. J Appl Bot Food Qual 89: 150-155. DOI: 10.5073/jabfq.2016.089.018.
Huang YS, Nong SY, Li XK, Xie G, Tong YH. 2022. Vaccinium bangliangense, a new species of Ericaceae from limestone areas in Guangxi, China. PhytoKeys 194: 23-31. DOI: 10.3897/phytokeys.194.81018.
Hussain S, Sharma S, Bhatti RC, Singh AN. 2023. Floral diversity expedition in Ladakh: An insight into the exploration, distribution pattern, ethnobotanical, phytochemical studies and conservation strategies. Ethnobot Res Appl 26: 1-32. DOI: 10.32859/era.26.43.1-32.
Jia G, Zhao H, Hou D, Sun T, Lin W. 2019. Quantitative determination of total flavonoids from Polygonatum sibiricum by spectrophotometry. IOP Conf Ser: Mater Sci Eng 677: 022126. DOI: 10.1088/1757-899X/677/2/022126.
Jurikova T, Skrovankova S, Mlcek J, Balla S, Snopek L. 2019. Bioactive compounds, antioxidant activity, and biological effects of European cranberry (Vaccinium oxycoccos). Molecules 24 (1): 24. DOI: 10.3390/molecules24010024.
Kaškonien? V, Bimbirait?-Survilien? K, Kaškonas P, Tiso N, ?esonien? L, Daubaras R, Maruška AS. 2020. Changes in the biochemical compounds of Vaccinium myrtillus, Vaccinium vitis-idaea, and forest litter collected from various forest types. Turk J Agric For 44: 557-566. DOI: 10.3906/tar-1912-41.
Liu P, Lindstedt A, Markkinen N, Sinkkonen J, Suomela J-P, Yang B. 2014. Characterization of metabolite profiles of leaves of bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea L.). J Agric Food Chem 62 (49): 12015-12026. DOI: 10.1021/jf503521m.
Mahajan M, Kuiry R, Pal PK. 2020. Understanding the consequence of environmental stress for accumulation of secondary metabolites in medicinal and aromatic plants. J Appl Res Med Aromat Plants 18: 100255. DOI: 10.1016/j.jarmap.2020.100255.
Nakhutsrishvili G, Batsatsashvili K, Bussmann RW, Ur Rahman I, Hart RE, Haq SM. 2022. The subalpine and alpine vegetation of the Georgian Caucasus-a first ethnobotanical and phytosociological synopsis. Ethnobot Res Appl 23: 1-60. DOI: 10.32859/era.23.12.1-60.
Petru?ová V, Ba?korová M. 2024. Determination of phytochemical quality of leaves Vaccinium vitis-idaea L. and Vaccinium myrtillus L. from the polluted and non-polluted areas. Environ Monit Assess 196 (11): 1135. DOI: 10.1007/s10661-024-13157-1.
Raudone L, Vilkickyte G, Pitkauskaite L, Raudonis R, Vainoriene R, Motiekaityte V. 2019. Antioxidant activities of Vaccinium vitis-idaea L. leaves within cultivars and their phenolic compounds. Molecules 24 (5): 844. DOI: 10.3390/molecules24050844.
Reshi ZA, Ahmad W, Lukatkin AS, Bin Javed S. 2023. From nature to lab: A review of secondary metabolite biosynthetic pathways, environmental influences, and in vitro approaches. Metabolites 13 (8): 895. DOI: 10.3390/metabo13080895.
Rifnas LM, Vidanapthirana N. 2023. Plant Morphology. University of Colombo Institute for Agro-Technology and Rural Sciences, Sri Lanka.
Roziaty E, Nurrahman Al Farisi A. 2022. Cantigi (Vaccinium varingifolium (Blume) Miq.) di jalur pendakian Gunung Lawu Kabupaten Karanganyar Jawa Tengah. Bioeksperimen 8 (1): 20-30. DOI: 10.23917/bioeksperimen.v8i1.18086. [Indonesian]
Stanisci A, Bricca A, Calabrese V, Cutini M, Pauli H, Steinbauer K, Carranza ML. 2020. Functional composition and diversity of leaf traits in subalpine versus alpine vegetation in the Apennines. AoB Plants 12 (2): plaa004. DOI: 10.1093/aobpla/plaa004.
?tef?nescu B-E, C?linoiu LF, Ranga F, Fetea F, Mocan A, Vodnar DC, Cri?an G. 2020. Chemical composition and biological activities of the nord-west romanian wild bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea L.) leaves. Antioxidants 9 (6): 495. DOI: 10.3390/antiox9060495.
Svanberg I, Ståhlberg S. 2021. Wild European dewberry, Rubus caesius L. (fam. Rosaceae), in Sweden: From traditional regional consumption to exotic dessert at the Nobel Prize banquet. J Ethnic Foods 8: 37. DOI: 10.1186/s42779-021-00114-3.
Tong Y-H, Huang Y-S, Ye X-H, Cai Z-Y, Xia N-H. 2020. Vaccinium napoense, a new species of V. sect. Conchophyllum (Ericaceae) from Guangxi, China. Nordic J Bot 38 (12): e02773. DOI: 10.1111/njb.02773.
Tong Y-H, Zhu Y-Y, Ye X-H, Ye X-E, Yang C-Z, Xia N-H. 2021. Vaccinium zhangzhouense, a new species endemic to Fujian, China. Nordic J Bot 39 (7): e03091. DOI: 10.1111/njb.03091.
Torres-Guevara FÁ, Ganoza-Yupanqui ML, Mantilla-Rodriguez E, Suárez-Rebaza LA, Bussmann RW. 2023. Ethnobotany of fruit species native to paramos and cloud forests of Northern Peru. Ethnobot Res Appl 25: 1-15. DOI: 10.32859/era.25.10.1-15.
Vilkickyte G, Raudone L. 2021. Phenological and geographical effects on phenolic and triterpenoid content in Vaccinium vitis-idaea L. leaves. Plants 10 (10): 1986. DOI: 10.3390/plants10101986.
Wang L, Wang J, Tan X, Fang C. 2020. Analysis of NOx pollution characteristics in the atmospheric environment in Changchun City. Atmosphere 11(1). DOI: 10.3390/ATMOS11010030
Yu F, Groen TA, Wang T, Skidmore AK, Huang J, Ma K. 2017. Climatic niche breadth can explain variation in geographical range size of alpine and subalpine plants. Intl J Geogr Inf Sci 31 (1): 190-212. DOI: 10.1080/13658816.2016.1195502.
Yulyana A, Chaidir C, Simanjuntak P, Sulastri L, Abdillah S. 2023. The water fraction of Cantigi (Vaccinium varingiaefolium Bl. Miq.) fruits demonstrate the highest antimetabolic syndrome properties on enzyme assay. Pharmacia 70: 587-594. DOI: 10.3897/pharmacia.70.e109333.
Yulyana A, Winarno H, Kosasih K. 2016. Karakterisasi ekstrak daun cantigi (Vaccinium varingiaefolium Miq.). Jurnal Sains dan kesehatan 1 (5): 276-283.
Zehnder T, Lüscher A, Ritzmann C, Pauler CM, Berard J, Kreuzer M, Schneider MK. 2020. Dominant shrub species are a strong predictor of plant species diversity along Subalpine pasture-shrub transects. Alp Bot 130: 141-156. DOI: 10.1007/s00035-020-00241-8.
Zhang L, Liu P, Li L, Huang Y, Pu Y, Hou X, Song L. 2019. Identification and antioxidant activity of flavonoids extracted from xinjiang jujube (Ziziphus jujube Mill.) leaves with ultra-high pressure extraction technology. Molecules 24 (1): 122. DOI: 10.3390/molecules24010122.
Zhou Y, Yang M, Tai Z, Jia J, Luan D, Ma X. 2022. Carbohydrates and secondary compounds of alpine tundra shrubs in relation to experimental warming. BMC Plant Biol 22: 482. DOI: 10.1186/s12870-022-03851-y.
Zoratti L, Jaakola L, Häggman H, Giongo L. 2015. Anthocyanin profile in berries of wild and cultivated Vaccinium spp. along altitudinal gradients in the Alps. J Agric Food Chem 63 (39): 8641-8650. DOI: 10.1021/acs.jafc.5b02833.