Segregation analysis and floral phenotype of T2 transgenic yellow cosmos (Cosmos sulphureus Cav.) carrying neomycin phosphotransferase II gene in second generation
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Muchyiddin MGAA, Sawitri WD, Irsyadi MB, Swandari T, Purwantoro A. 2024. Segregation analysis and floral phenotype of T2 transgenic yellow cosmos (Cosmos sulphureus Cav.) carrying neomycin phosphotransferase II gene in second generation. Biodiversitas 25: 1711-1717. The seed of transgenic yellow cosmos (Cosmos sulphureus Cav.) lines harbouring neomycin phosphotranferase II (nptII) gene have been obtained from our previous studies. The nptII selectable marker gene is commonly used for developing a method to estimate the number of targeted gene integrated into the genome. In order to produce successful clones, it is important to ensure that transgenes are inherited sexually as the dominant trait in T2 progeny. This study aimed to obtain the segregation patterns of nptII gene in two populations derived from open- and self-pollinated and evaluated floral phenotypic of T2 transgenic yellow cosmos. The molecular analysis of transgenic yellow cosmos was conducted by PCR and the obtained data was analyzed using a Chi-Square test. The results showed that transgenes were inherited in 1:1 for transgenic and wild-type in both self- and open-pollinated populations. Therefore, it indicated that T2 transgenic plants were still unstable and evolved continuously to segregate independently in the over generation. Surprisingly, the morphological changes were detected in transgenic plants. The mixture of ligulate and tubular types in ray floret was found in some of the flower types in T2 yellow cosmos. In this case, nptII gene may diverge in apparently random genome insertion. This insertion would induce morphological changes in the ray floret type of flower since recombination estimates varied among transformation events.
##plugins.themes.bootstrap3.article.details##
Most read articles by the same author(s)
- SUYITNO ALOYSIUS, AZIZ PURWANTORO, KUMALA DEWI, ENDANG SEMIARTI, Improvement of genetic variability in seedlings of Spathoglottis plicata orchids through X-ray irradiation , Biodiversitas Journal of Biological Diversity: Vol. 18 No. 1 (2017)
- MUHAMAD JALIL, AZIZ PURWANTORO, BUDI SETIADI DARYONO, PURNOMO, Distribution, variation, and relationship of Curcuma soloensis Valeton in Java, Indonesia based on morphological characters , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 8 (2020)
- DYAH WENY RESPATIE, PRAPTO YUDONO, AZIZ PURWANTORO, Y. ANDI TRISYONO, The potential of Cosmos sulphureus flower extract as a bioherbicide for Cyperus rotundus , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 12 (2019)
- Siti Yuli Meilanda Sormin, Aziz Purwantoro, Agus Budi Setiawan, Chee How Teo, Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 5 (2021)
- THERESA D. KURNIA, AZIZ PURWANTORO, SRI SULANDARI, PANJISAKTI BASUNANDA, AGUS B. SETIAWAN, YENI FATMAWATI, IGNATIUS P. ANDIKA, Molecular and morpho-physiological identification of yellow leaf curl disease of cucumber in Salatiga, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 3 (2022)
- YENI FATMAWATI, AGUS BUDI SETIAWAN, AZIZ PURWANTORO, DYAH WENY RESPATIE, CHEE HOW TEO, Analysis of genetic variability in F2 interspecific hybrids of mung bean (Vigna radiata) using inter-retrotransposon amplified polymorphism marker system , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 11 (2021)
- SUYITNO ALOYSIUS, AZIZ PURWANTORO, KUMALA DEWI, ENDANG SEMIARTI, Phenotypic variation and genetic alteration of Spathoglottis plicata resulted from in vitro cultured seed irradiated with X-Ray , Biodiversitas Journal of Biological Diversity: Vol. 19 No. 5 (2018)
- HERNI SHINTIAVIRA, ENDANG SULISTYANINGSIH, AZIZ PURWANTORO, RANI AGUSTINA WULANDARI, The effects of gibberellic acid (GA3) on the harvesting time of spray type Chrysanthemum cut flowers in medium land , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 4 (2020)
- AINUN NIKMATI LAILY, BUDI SETIADI DARYONO, AZIZ PURWANTORO, PURNOMO, Various macro and micro-morphological characters of three sex types of highland papaya (Vasconcellea pubescens) in Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 12 (2022)