Morphological and anatomical studies of Conamomum vietnamense N.S. Lý & T.S. Hoang: An endemic plant from Vietnam

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DOI https://doi.org/10.13057/biodiv/d240946
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Vol. 24 No. 9 (2023)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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NGUYEN THANH TO NHI
Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City. No. 300A, Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh City 70000, Vietnam
TRAN VAN CHEN ♥
Faculty of Traditional Medicine, University of Medicine and Pharmacy at Ho Chi Minh City. No.217, Hong Bang Street, Ward 11, District 5, Ho Chi Minh City 70000, Vietnam
https://orcid.org/0000-0003-1430-231X
DUONG NGUYEN XUAN LAM
Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City. No. 217, Hong Bang Street, Ward 11, District 5, Ho Chi Minh City 70000, Vietnam
DANH DUC NGUYEN
Institute of Applied Technology, Thu Dau Mot University. No. 06, Tran Van On Street, Phu Hoa Ward, Thu Dau Mot City 7510, Binh Duong Province, Vietnam
QUANG DIEP DINH
Nong Lam University. Quarter 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City 70000, Vietnam
https://orcid.org/0000-0002-1462-0070
NGUYEN HOANG KHANH LINH
Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

Abstract

Abstract. Nhi NTT, Chen TV, Lam DNX, Nguyen DD, Dinh QD, Linh NHK. 2023. Morphological and anatomical studies of Conamomum vietnamense N.S. Lý & T.S. Hoang: An endemic plant from Vietnam. Biodiversitas 24: 5022-5034. Conamomum vietnamense N.S. Lý & T.S. Hoang (Zingiberaceae) is an endemic plant from Vietnam. Information on the anatomical structure of leaves, roots, rhizomes, and the powder characteristics of C. vietnamense has not been reported. This study aimed to combine macro- and micro-morphological analysis to identify C. vietnamense accurately. Twenty fresh plants were randomly collected from the wild habitats in Vietnam were used for the study. Macromorphological characteristics were observed and compared to earlier research. Iodine green-carmine staining was then used to histologically examine the leaves, roots, and rhizomes. The powdered rhizome and leaf features were described via a microscope. Macromorphologically, C. vietnamense is characterized by its stout stilt roots, short petioles, oblong-lanceolate leaf blades with lightly plicate, inflorescences with obovoid, and narrowly ovate bracts, hairy underside bracteoles, bilobed calyx, yellow flowers with broadly obovate, glabrous, trilobed labellum. Micromorphologically, the anatomical features of leaves, roots, and rhizomes have similarities with those of Zingiberaceae species. The tetracytic stomata was observed on both leaf surfaces. The stomatal indexes on the abaxial and adaxial surfaces of the leaves are 8.81±0.55% and 0.1±0.06%, respectively. Calcium oxalate crystals were found in the leaf. Starch grains and reticulate xylem vessels were present only in rhizome powder. These findings are important characteristics for identifying C. vietnamense.

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References
Abdulrahaman AA, Taiwo MO, Oladele FA. 2015. Phytopharmaceutical potential and microscopic analysis of rhizomes of Curcuma longa and Zingiber officinale (Zingiberaceae). Ann West Univ Timi? Ser Biol 18(2): 73-86.
Ahmed SS, Kumar R. 2022. Pharmacognostical standardization of Cassia auriculata, Centella asiatica and Zingiber officinale. J Med Pharm Allied Sci 11(5): 5227-5231. DOI: 10.55522/jmpas.V11I5.4060.
Alamgir ANM. 2017. Pharmacognostical Botany: Classification of Medicinal and Aromatic Plants (MAPs), Botanical Taxonomy, Morphology, and Anatomy of Drug Plants. In: Therapeutic Use of Medicinal Plants and Their Extracts: Volume 1. Progress in Drug Research, vol 73, 177-293. Springer, Cham. DOI:10.100¬7/978-3-319-63862-1_6.
Amel B. 2015. Microscopic analysis of Curcuma longa L. using multivariate test. Int J Pharmacol 2(4): 173-177. DOI:10.13040/IJPSR.0975-8232.IJP.2(4).173-77.
Anu S, Navas M, Dan M. 2020. Morpho-anatomical characterisation of the rhizomes of ten species of Curcuma L. (Zingiberaceae) from South India. J Spices Aromatic Crops 29(1): DOI: 10.25081/josac.2020.v29.i1.6243.
Boer HD, Newman M, Poulsen AD, Droop AJ, Fér T, Thu Hi?n LT, Hlavatá K, Lamxay V, Richardson JE, Steffen K, Leong-Škorni?ková J. 2018. Convergent morphology in Alpinieae (Zingiberaceae): Recircumscribing Amomum as a monophyletic genus. Taxon 67 (1): 6–36. DOI: 10.12705/671.2.
Das A, Pal KK, Nag S. 2018. Anatomy, micromorphology and histochemical localization of different phytochemicals of two medicinally important taxa of the family Zingiberaceae. Res J Life Sci Bioinform Pharm Chem Sci 4(1):190-198. DOI: 10.26479/2018.0401.16.
Gevú KV, Da Cunha M, Barros CF, Pereira SM, Lima HRP. 2014. Structural analysis of subterranean organs in Zingiberaceae. Plant Syst Evol 300(5): 1089-1098. DOI: 10.1007/s00606-013-0947-y.
He G, Yang SB, Wang YZ. 2023. The potential of Amomum tsao-ko as a traditional Chinese medicine: traditional clinical applications, phytochemistry and pharmacological properties. Arab J Chem 16(8):104936. DOI:10.1016/j.arabjc.2023.104936.
Kajornjit P, Saensouk S, Saensouk P. 2018. Pollen morphology and leaf anatomy of genus Globba in Thailand. Sci Asia 44(3): 146-161. DOI: 10.2306/scienceasia1513-1874.2018.44.146.
Lamxay V, Newman MF. 2012. A revision of Amomum (Zingiberaceae) in cambodia, laos and vietnam. Edinb J Bot 69(1): 99-206. DOI: 10.1017/S0960428611000436.
Liang H, Zhang Y, Deng J, Gao G, Ding C, Zhang L, Yang R. 2020. The complete chloroplast genome sequences of 14 Curcuma species: Insights into genome evolution and phylogenetic relationships within Zingiberales. Front Genet 11:e802. DOI: 10.3389/fgene.2020.00802.
Liu H, Specht CD, Zhao T, Liao J. 2020. Morphological anatomy of leaf and rhizome in Zingiber officinale Roscoe, with emphasis on secretory structures. Hort Sci 55 (2): 204-207. DOI: 10.21273/HORTSCI14555-19.
Luu DNA, Bui VH, Luu DC, Nguyen QH, Trinh XT, Thanh TN. 2021. Conamomum pierreanum (Gagnep.) Skornick. & AD Poulsen (Zingiberaceae), a New Record for the Flora of Vietnam. VNU Journal of Science: Natural Sciences and Technology 37(2): 41-45. DOI: 10.25073/2588-1140/vnunst.5035.
Ly NS, Hoang TS, Insisiengmay O, Haevermans T, Newman MF. 2022. Conamomum vietnamense (Zingiberaceae), a new species from Tay Nguyen, Vietnam. Phytotaxa 531(2): 129-135. DOI: 10.11646/phytotaxa.531.2.5.
Mathew S, Britto SJ, Thomas S. 2014. Comparative powder microscopical screening of the rhizome and leaf of Alpinia calcarata and Alpinia galanga. Intl J Pharm Sci Res 5(4): 1449. DOI: 10.13040/IJPSR.0975-8232.5(4).1449-53.
Ministry of Health. 2017. Vietnamese Pharmacopoeia V. Medical Publishing House. Ha Noi, Vietnam.
Muyumba NW, Mutombo SC, Sheridan H, Nachtergael A, Duez P. 2021. Quality control of herbal drugs and preparations: The methods of analysis, their relevance and applications. Talanta Open 4:100070. DOI: 10.1016/j.talo.2021.100070.
Nguyen DD, Nguyen NH, Tran TH, Nguyen DK, Thi Nguyen LT. 2023. Limonene and eucalyptol rich essential oils with their antimicrobial activity from the leaves and rhizomes of Conamomum vietnamense N.S. Lý & T.S. Hoang (Zingiberaceae). Pharmacia 70(1): 91-96. DOI:10.3897/pharmacia.70.e96946.
Nunes TD, Zhang D, Raissig MT. 2020. Form, development and function of grass stomata. Plant J 101(4): 780-799. DOI: 10.1111/tpj.14552.
Osman AG, Raman V, Haider S, Ali Z, Chittiboyina AG, Khan IA. 2019. Overview of analytical tools for the identification of 3 adulterants in commonly traded herbs and spices. J AOAC Intl 102(2): 376-385. DOI: 10.5740/jaoacint.18-0389.
Paopun Y. 2020. Calcium oxalate crystals and leaf anatomical characteristics of Kaempferia galanga L. J Microsc Soc Thail 33(1): 28-33. DOI: 10.14456/microsc-microanal-res.2020.7.
Roy B, Jana BK, Maiti GG. 2013. Morpho-anatomical diversity of the rhizomes of some medicinal and aromatic plants of Zingiberaceae. Int J Chem Pharm Res 2(8): 197-203.
Roy S, Acharya RN, Harisha CR, Shukla VJ. 2016. Macro, microscopic and preliminary analytical evaluation of root and leaf of Globba marantina Linn.- An extrapharmacopoeial drug of Ayurveda. Indian J Pharm Sci 78(4): 469-478. DOI: 10.4172/pharmaceutical- sciences.1000141.
Salasiah M, Meekiong K. 2018. Preliminary anatomical study on leaf surfaces of Bornean Zingiberaceae (tribe Alpinieae) from northeast Sarawak. Malays Appl Biol 47(5): 289-293.
Santos Tozin LRD, de Melo Silva SC, Rodrigues TM. 2016. Non-glandular trichomes in Lamiaceae and Verbenaceae species: morphological and histochemical features indicate more than physical protection. N Z J Bot 54(4): 446-457. DOI: 10.1080/0028825X.2016.1205107.
Segev R, Nannapaneni R, Sindurakar P, Kim H, Read H, Lijek S. 2015. The effect of the stomatal index on the net rate of photosynthesis in the leaves of Spinacia oleracea, Vinca minor, Rhododendron spp., Epipremnum aureum, and Hedera spp.. J Emerg Investig 2015 (20): 1-5.
Sharifi-Rad M, Varoni EM, Salehi B, Sharifi-Rad J, Matthews KR, Ayatollahi SA, Rigano D. 2017. Plants of the genus Zingiber as a source of bioactive phytochemicals: From tradition to pharmacy. Molecules 22(12): 2145. DOI: 10.3390/molecules22122145.
Shukla Y, Singh M. 2007. Cancer preventive properties of ginger: A brief review. Food Chem Toxicol 45(5): 683–690. DOI: 10.1016/j.fct.2006.11.002.
Srivastava S, Misra A. 2018. Quality Control of Herbal Drugs: Advancements and Challenges. In: Singh B, Peter K. (eds) New Age Herbals: Resource, Quality and Pharmacognosy, Springer, Singapore, 189-290. DOI: 10.1007/978-981-10-8291-7_10. [Singapore]
Tang Y, Liao J, Wu Q. 2010. Analyses of anatomic structure and their systematic significances of Zingiberaceous roots of 28 species in China. Acta Bot Boreal Occid Sin 30(9): 1821-1833.
Truong LH, Dang TH, Dat NQ, Trung NTQ, Gioi T. 2019. Conamomum odorum, a new species of Zingiberaceae from central Vietnam. Academia J Biol 41(3): 55–59. DOI: 10.15625/2615-9023/v41n3.13671.
Uma E, Muthukumar T. 2014. Comparative root morphological anatomy of Zingiberaceae. Syst Biodivers 12(2): 195-209. DOI: 10.1080/14772000.2014.894593.
Van Chen T, Lam DNX, Thong CLT, Nguyen DD, Nhi NTT, Triet NT. 2022b. Morphological characters, pharmacognostical parameters, and preliminary phytochemical screening of Curcuma sahuynhensis Škorni?k. & N.S. Lý in Quang Ngai Province, Vietnam. Biodiversitas 23 (8): 3907-3920. DOI: 10.13057/biodiv/d230807.
Van Chen T, Tuan ND, Triet NT, An NH, Nguyen PTT, Hai NTT, Nhi NTT, Co NQ, Nhi HTH, Huong HV, Phuong TTB, Nhung NTA. 2022a. Morphological and molecular characterization of Distichochlamys citrea M.F. Newman in Bach Ma National Park, Thua Thien Hue Province, Vietnam. Biodiversitas 23(4): 2066-2079. DOI: 10.13057/biodiv/d230442.
Wang P, Huang F, Zhou L, Cao L, Liang S, Xu H, Liu J. 2000. Analysis of Amomun villosum species and some adulterants of Zingiberaceae by RAPD. J Chin Med Mater 23(2): 71-74.
WHO. 2011. Quality Control Methods for Medicinal Plant materials. WHO Press. Geveva.
Wijayasiriwardena C, Premakumara S. 2012. Comparative powder microscopy of Alpinia calcarata Roscoe and Alpinia galanga (Linn.) Willd. Ayu 33(3): 441. DOI: 10.4103/0974-8520.108863.
Wijayasiriwardene TDMCK, Herath HMIC, Premakumara GAS. 2016. Morphological & microscopic identification of Curcuma albiflora Thw. J Ayu Her Med 2(1): 15-19. DOI: 10.31254/jahm.2016.2105.
Windarsih G, Riastiwi I, Dewi AP, Yuriyah S. 2022. Stomatal and epidermal characteristics of Zingiberaceae in Serang District, Banten, Indonesia. Biodiversitas 23(10): 5373-5386. DOI: 10.13057/biodiv/d231048.
Windarsih G, Utami DW, Yuriyah S. 2021. Morphological characteristics of Zingiberaceae in Serang District, Banten, Indonesia. Biodiversitas 22(12): 5507-5529. DOI: 10.13057/biodiv/d221235.
Wu MH, Zhang W, Guo P, Zhao ZZ. 2014. Identification of seven Zingiberaceous species based on comparative anatomy of microscopic characteristics of seeds. Chin Med 9(1): 1-7. DOI: 10.1186/1749-8546-9-10.
Xiao K, Mao X, Lin Y, Xu H, Zhu Y, Cai Q, Xie H, Zhang J. 2017. Trichome, a Functional Diversity Phenotype in Plant. Mol Biol 6(1):183. DOI: 10.4172/2168-9547.1000183.
Yang L, Feng C, Cai MM, Chen JH, Ding P. 2020. Complete chloroplast genome sequence of Amomum villosum and comparative analysis with other Zingiberaceae plants. Chin Herb Med 12(4): 375-383. DOI: 10.1016/j.chmed.2020.05.008.
Zahara M. 2020. Identification of morphological and stomatal characteristics of Zingiberaceae as medicinal plants in Banda Aceh, Indonesia. In: Samadi, Yunita D, Goritno AD (eds). IOP Conf Ser: Earth Environ Sci. The 1st International Conference on Agriculture and Bioindustry, Banda Aceh, Indonesia, 24-26 October 2019. DOI: 10.1088/1755-1315/425/1/012046. [Indonesian]
Zhao H, Xiao MH, Zhong Y, Wang YQ. 2022. Leaf epidermal micromorphology of Zingiber (Zingiberaceae) from China and its systematic significance. PhytoKeys 190: 131-146. DOI: 10.3897/phytokeys.190.77526.
Zhao Z, Liang Z, Ping G. 2011. Macroscopic identification of Chinese medicinal materials: traditional experiences and modern understanding. J Ethnopharmacol 134(3): 556-564. DOI: 10.1016/j.jep.2011.01.018.
Zhao ZZ, Hu YN, Wong YW, Wong WCG, Wu KIM, Jiang ZH, Kang T. 2005. Application of microscopy in authentication of Chinese patent medicine—Bo Ying compound. Microsc Res Tech 67(6): 305-311. DOI: 10.1002/jemt.20213.

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