Probiotics potential of lactic acid bacteria isolated from Slender Walking Catfish (Clarias nieuhofii)
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
RAHMAD LINGGA
♥
https://orcid.org/0000-0002-2787-3819
SUDIRMAN ADIBRATA
OCCA ROANISCA
SIPRIYADI
RISKY HADI WIBOWO
AHMAD ARSYADI
Abstract
Abstract. Lingga R, Adibrata S, Roanisca O, Sipriyadi, Wibowo RH, Arsyadi. 2023. Probiotics potential of lactic acid bacteria isolated from Slender Walking Catfish (Clarias nieuhofii). Biodiversitas 24: 4572-4580. Probiotics are living microorganisms or bioactive agents that positively impact animal digestion. They have successfully isolated from various sources. Recently, we isolated and characterized lactic acid bacteria (LAB) from slender walking fish (Clarias nieuhofii Valenciennes, 1840). The fish sample was obtained from Batu Rusa and Paya Benua Rivers in Bangka Island, Indonesia. LAB was isolated from the fish intestine using the pour plate method. The isolated LAB was then characterized based on their phenotypic traits, biochemical properties, and the 16S rRNA gene identification. The chosen isolates were tested to determine their ability to produce lactic acid, hemolytic and antibacterial activity, and antibiotic resistance. All isolates have the characteristic of rod-shaped and short rod-shaped cells with Gram-positive properties. Isolate KP1 showed the number of populations (2.89 x 107 CFU/mL) and lactic acid production with a concentration of 1.85%. All isolates exhibited no hemolysis activity and displayed sensitivity to antibiotics. The twelve lactic acid bacteria formed clear zones against Staphylococcus aureus and Escherichia coli. The results of the 16S rRNA gene identification indicated that isolates KB4, KB7, KB8, and KP1 belong to Lactobacillus vaginalis, Lactobacillus fermentum, Limosilactobacillus fermentum, and Levilactobacillus brevis, respectively. Lactic acid bacteria isolated from slender walking fish exhibited potential probiotic traits.
##plugins.themes.bootstrap3.article.details##
References
Abedi E, Hashemi SMB. 2020. Lactic acid production–producing microorganisms and substrates sources-state of art. Heliyon 6(2020): 1-32. DOI: https://doi.org/10.1016/j.heliyon.2020.e04974.
Adeli MM, Kazempoor R, Shirazi NH. 2022. Effect of probiotic Lactobacillus fermentum on growth performance, bioaccumulation and antioxidant defenses of zebrafish (danio rerio) under cadmium toxicity. Aquaculture Studies 23(4): 1-10. DOI: http://doi.org/10.4194/AQUAST991
Agustina A, Saptian G, Hardi EH. 2022. Isolation and identification of potential lactic acid bacteria as probiotics from the intestines of repang fish (Puntioplites waandersi). AACL Bioflux 15(1): 24-33.
Ahmed T, Kanwal R, Ayub N. 2006. Influence of temperature on growth pattern of Lactococcus lactis, Streptococcus cremoris and Lactobacillus acidophilus isolated from camel milk. Biotechnology 5(4): 481-488. DOI: 10.3923/biotech.2006.481.488.
Allameh S. K., Ringø E., Yusoff F. M., Daud H. M., Ideris A. 2014 Properties of Enterococcus faecalis, a new probiotic bacterium isolated from the intestine of snakehead fish (Channa striatus Bloch). African Journal of Microbiology Research 8(22):2215-2222.
American Society for Microbiology. 2016. Blood Agar Plates and Hemolysis Protocols.
Anisimova E, Gorokhova I, Karimullina G, Yarullina D. 2022. Alarming antibiotic resistance of Lactobacilli isolated from probiotic preparations and dietary supplements. Antibiotics 11: 1-12. DOI: https://doi.org/10.3390/antibiotics11111557.
Ayivi RD, Ibrahim SA. 2022. Lactic acid bacteria: an essential probiotic and starter culture for the production of yoghurt. International Journal of Food Science and Technology 57: 7008–7025. DOI: 10.1111/ijfs.16076.
Bayu HH, Irwanto R, Dalimunthe NP, Lingga R. 2023. Isolation and identification of lactic acid bacteria from Channa sp. as potential probiotic. Jurnal Pembelajaran dan Biologi Nukleus 9(1): 75-84.
Bernardeau M, Vernoux JP, Dubernet SH, Gueguen M. 2008. Safety assessment of dairy microorganisms: The Lactobacillus genus. International Journal of Food Microbiology 126: 278–285.
Denev, S. A. 2008. Ecological alternatives of antibiotic growth promoters in the animal husbandry and aquaculture. DSc thesis, Department of Biochemistry Microbiology, Trakia University Stara Zagora, Bulgaria (pp. 294).
Emanuel V, Adrian V, Ovidiu P, Gheorghe C. 2005. Isolation of a Lactobacillus plantarum strain used forobtaining a product for the preservation of fodders. African Journal of Biotechnology 4(5): 403-408.
Faeed M, Kasra KR, Pourkazemi M, Ahmadnezhad M. 2020. In vivo study on probiotic Lactobacillus brevis in Sander lucioperca and some of their nonspecific immune parameters, intestinal morphology and survival against Aeromonas hydrophila.. Iranian Journal of Fisheries Sciences 21(2): 387-402. DOI: 10.22092/ijfs.2022.126354.
Ghanbari M, Rezaei M, Jami M, Nazari RM. 2009. Isolation and characterization of Lactobacillus speciesfrom intestinal contents of beluga (Huso huso) and Persian sturgeon (Acipenser persicus). Iranian Journal of Veterinary Research 10(2): 152-157.
Govindaraj K, Samayanpaulraj V, Narayanadoss V, Uthandakalaipandian R. 2021. Isolation of lactic acid bacteria from intestine of freshwater fishes
and elucidation of probiotic potential for aquaculture application. Probiotics and Antimicrobial Proteins 13: 1598–1610. DOI: 10.1007/s12602-021-09811-6.
Haro G, Iksen I, Nasri N. 2020. Identification, characterization and antibacterial potential of probiotic lactic acid bacteria isolated from naniura (A traditional Batak fermented food from carp) against Salmonella typhi. Rasayan J Chem 13(1): 464-468. DOI: 10.31788/RJC.2020.1315530.
Harzallah D, Belhadj. 2013. Characteristics, selection criteria and role in immunomodulation of human gi muccosal barrier. Intechopen chapter 8: 197–216. DOI: 10.5772/50732.
Hoseinifar SH, Ringø E, Masouleh AS, Esteban MA. 2016. Probiotic, prebiotic and synbiotic supplements in sturgeon aquaculture: a review. Rev Aquacult 8:89-102.
Hernentis H, Marlida Y, Nur YS, Wizna W, Santi MA, Septiani N, Adzitey F, Huda N. 2020. Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia. Vet World 13(9): 1922–1927. DOI: 10.14202/vetworld.2020.1922-1927.
Husain F, Duraisamy S, Balakrishnan S, Ranjith S, Chidambaram P, Kumarasamy A. 2022. Phenotypic assessment of safety and probiotic potential of native isolates from marine fish Moolgarda seheli towards sustainable aquaculture. Biologia 77:775-790.
Kim, G. B., Seo, Y. M., Kim, C. H., & Paik, I. K. 2011. Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poultry Science 90(1): 75–82. https://doi.org/10.3382/ps.2010-00732.
Kurnia M, Amir H, Handayani D. 2020. Identifikasi Bakteri Asam Laktat dari Makanan Tradisional Suku Rejang di Provinsi Bengkulu: Lemea. J. Pendidikan dan Ilmu Kimia 4(1): 25-32.
Lara-Flores M., Olvera-Novoa M. A.. 2013 The use of lactic acid bacteria isolated from intestinal tract of Nile tilapia (Oreochromis niloticus), as growth promoters in fish fed low protein diets. Latin American Journal of Aquatic Research 41(3):490-497.
Lazado CC, Caipang CM. 2014. Mucosal immunity and probiotics in fish. Fish Shellfish Immunol 39 (1):78-89. https://doi.org/10.1016/j.fsi.2014.04.015.
Llewellyn, M. S., Boutin, S., Hoseinifar, S. H., & Derome, N. 2015. Teleost microbiomes: The state of the art in their characterization, manipulation and importance in aquaculture and fisheries. Frontiers in Microbiology 207:1-18.
Loh J. Y., Lim Y. Y., Harmin S. A., Ting A. S. Y., 2014 In vitro assessment on intestinal microflora from commonly farmed fishes for control of the fish pathogen Edwardsiella tarda. Turkish Journal of Veterinary and Animal Sciences 38(3):257- 263.
Lokapirnasari WP, Sahidu AM, Soepranianondo K, Supriyanto A, Yulianto AB, Arif AA. 2018. Potency of lactic acid bacteria isolated from balinese bovine (Bos sondaicus) intestinal waste from slaughterhouse to improve nutrient content of wheat pollard as animal feedstuff by fermentation process. Vet World 11(8): 1127–1134. DOI: 10.14202/vetworld.2018.1127-1134.
Mulyasari, Widanarni, Suprayudi MA, Zairin Jr M., Sunarno MTD. 2016. Screening of probiotics from the digestive tract of gouramy (Osphronemus goramy) and their potency to enhance the growth of tilapia (Oreochromis niloticus). AACL Bioflux 9 (5):1121-1132.
Munir MB, Marsh TL, Blaud A, Hashim R, Joshua WJA, Nor SAM. 2017. Analysing the effect of dietary prebiotics and probiotics on gut bacterial richness and diversity of Asian snakehead fingerlings using T?RFLP method. Aquaculture Research 49:3350–3361. DOI: 10.1111/are.13799.
Muthukumar P., Kandeepan C. 2015 Isolation, identification and characterization of probiotic organisms from intestine of fresh water fishes. International Journal of Current Microbiology and Applied Sciences 4(3):607-616.
Nasri, Harahap U, Silalahi J, Satria D. 2021. Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria. Biodiversitas 22(8): 3098-3104. DOI: 10.13057/biodiv/d220802.
Nayak SK. 2010. Probiotics and immunity: a fish perspective. Fish Shellfish Immunol. 29(1): 2-14. https://doi.org/10.1016/j.fsi.2010.02.017.
Ouchari L, Boukeskasse A, Bouizgarne B, Ouhdouch Y. 2019. Antimicrobial potential of actinomycetes isolated from the unexplored hot Merzouga desert and their taxonomic diversity. Biol Open 8 (2). DOI: 10.1242/bio.035410.
Pan X, Chen F, Wu T, Tang H, Zhao Z. 2009. The acid, bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT. Food Control 20: 598-602. DOI: 10.1016/j.foodcont.2008.08.019.
Prihanto AA, Nursyam H, Jatmiko YD, Hayati RL. 2020. Autochthonous acid-producing bacteria from catfish (Clarias sp.) with antibacterial activity against selected fish pathogens: a preliminary study. Int J Microbiol 2020: 1-5. DOI: 10.1155/2020/8526581.
Pundir R. K., Rana S., Kashyap N., Kaur A. 2013 Probiotic potential of lactic acid bacteria isolated from food samples: an in vitro study. Journal of Applied Pharmaceutical Science 3(3):85-93.
Salminen S, Wright AV, Ouwehand A. 2004. Lactic Acid Bacteria: Microbiology and Functional Aspects. 3rd edition. Revised and Expanded Marcel Dekker Inc, New York.
Sambrook J, Russell DW. 2001. Molecular Cloning a Laboratory Manual. 3rd ed. New York. Cold Spring Harbor Laboratory Pr.
Sánchez-Ortiz A. C., Luna-Gonzáles A., Campa-Córdova Á. I., Escamilla-Montes R., Flores-Miranda M. C., Mazón-Suástegui J. M.. 2015 Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming. Latin American Journal of Aquatic Research 43(1):123-136.
Van Doan H, Hoseinifar SH, Khanongnuch C, Kanpiengjai A, Unban K, Van Kim V, Srichaiyo S. 2018. Host-associated probiotics boosted mucosal and serum immunity, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture 491:94-100.
Vijayaram S, Kannan S, Muthukumar S. 2016. Isolation and characterization of probiotic bacteria isolated from diverse fish fauna of the trodden Vaigai river at Theni district. Journal of Chemical and Pharmaceutical Research 8(7): 883-889.
Wanka KM, Damerau T, Costas B, Krueger A, Schulz C, Wuertz S. 2018. Isolation and characterization of native probiotics for fish farming. BMC Microbiol 18(1):1-13. https://doi.org/10.1186/s12866-018-1260-2.
Widanarni, Tanbiyaskur. 2015 Application of probiotic, prebiotic and synbiotic for the control of streptococcosis in tilapia Oreochromis niloticus. Pakistan Journal of Biological Sciences 18(2):59-66.
Most read articles by the same author(s)
- RAHMAD LINGGA, SRI BUDIARTI, IMAN RUSMANA, ARIS TRI WAHYUDI, Isolation, characterization and efficacy of lytic bacteriophages against pathogenic Escherichia coli from hospital liquid waste , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 7 (2020)
- RENY HERAWATI, ALNOPRI, MASDAR, MARULAK SIMARMATA, SIPRIYADI, MIMI SUTRAWATI, Identification of Drought Tolerant and Molecular Analysis of DREB2A and BADH2 Genes and Yield Potensial of Lines from Single Crossing Bengkulu Local Rice Varieties , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 2 (2021)
- SUKMAWATI SUKMAWATI, FEBRIANTI ROSALINA, SIPRIYADI, NURUL KUSUMA DEWI, MELDA YUNITA, ABDUL RIDHA TAHA SARHAN, YENI RAHAYU, EKO KUSUMAWATI, Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 3 (2022)
- SUKMAWATI SUKMAWATI, SIPRIYADI, MELDA YUNITA, NURUL KUSUMA DEWI, ERIN D. NOYA, Analysis of bacteriocins of lactic acid bacteria isolated from fermentation of rebon shrimp (Acetes sp.) in South Sorong, Indonesia as antibacterial agents , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 7 (2022)
- SUKMAWATI SUKMAWATI, RATNA RATNA, SIPRIYADI, MELDA YUNITA, Characterization and molecular identification of bacteria from mackerel bekasam in Sorong City, Southwest Papua Province, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 9 (2023)
- BAYU PRANATA, RIDWAN SALA, ARADEA BUJANA KUSUMA, ABDUL HAMID A. TOHA, DEBORA CHRISTIN PURBANI, DANIEL FRIKLI MOKODONGAN, SIPRIYADI, Genetic diversity and connectivity of Red Snapper Lutjanus gibbus in the Papua Waters, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 1 (2024)
- DEBIE RIZQOH, SIPRIYADI, UTARI HARTATI SURYANI, CINTYA NABILA PUTRI, MEUTIA AGUSTIN, HERNITA TAURUSTYA, NOVRIANTIKA LESTARI, MARDHATILLAH SARIYANTI, Exploring the antibacterial activity of endophytic bacteria from Andaliman (Zanthoxylum acanthopodium DC.) against Bacillus subtilis , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 2 (2024)
- AHMAT FAKHRI UTAMA, JARULIS, SIPRIYADI, IZUL MIFTAKHUL JANNAH, DNA barcoding of Burgo chicken from Bengkulu, Indonesia, based on the cytochrome oxidase gene sub unit I mitochondria DNA , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 11 (2023)
- RISKY HADI WIBOWO, SIPRIYADI, WELLY DARWIS, DELFIANI ANGGIAS PUTRI, SALPRIMA YUDHA, MASHUDI, NOOR ANDRYAN ILSAN, PERSON PESONA RENTA, Isolation, characterization and identification of sponge-associated bacteria producing antimicrobial compounds , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 6 (2023)