Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia

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DOI https://doi.org/10.13057/biodiv/d240210
Issue
Vol. 24 No. 2 (2023)
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Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

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CHRISMIS NOVALINDA GINTING
Laboratory of Basic Sciences, Universitas Prima Indonesia. Jl. Sampul, Sei Putih Baru, Medan 20118, North Sumatra, Indonesia
FINNA PISKA
Faculty of Medicine, Laboratory of Basic Sciences, Universitas Prima Indonesia. Jl. Sampul, Sei Putih Baru, Medan 20118, North Sumatra, Indonesia
HARMILENI
Department of Chemical Engineering, Politeknik Teknologi Kimia Industri. Jl Medan Tenggara No. VII, Medan 20228, North Sumatra, Indonesia
EDY FACHRIAL ♥
Laboratory of Basic Sciences, Universitas Prima Indonesia. Jl. Sampul, Sei Putih Baru, Medan 20118, North Sumatra, Indonesia

Abstract

Abstract. Ginting CN, Piska F, Harmileni, Fachrial E. 2023. Molecular identification of thermophilic bacteria with antimicrobial activity isolated from hot springs in North Sumatra, Indonesia. Biodiversitas 24: 752-758. Microorganisms such as thermophilic bacteria are promising sources of antimicrobial compounds due to their abundance, which has yet to be widely investigated. Therefore, this study aims to identify thermophilic bacteria isolated from natural hot springs, Sidebuk Debuk, in North Sumatra, Indonesia. The bacteria were isolated from sediments and hot springs water. Isolated 10 bacteria were characterized and identified based on their morphological and biochemical properties, which included gelatinase activity, motility, citrate utilization, triple sugar iron agar test, catalase test, cell shape, color, and Gram staining. Antimicrobial activity was tested using the disc diffusion method against Escherichia coli and Staphylococcus aureus. The most potential isolates were identified molecularly by amplification of 16S rRNA genes using universal primers. The sequences result were trimmed and assembled using BioEdit and submitted to BLAST to determine the homology of the bacteria. The isolate ITU9 was identified as Bacillus megaterium and exhibited the best antimicrobial activity with an inhibition zone of 9.5 mm against S. aureus during 72 h of incubation. This research showed that the thermophilic B. megaterium strain ITU9 was successfully isolated from Sidebuk Debuk hot springs and had stable antimicrobial activity for 72 h against E. coli and S. aureus.

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References
Al-Thubiani, Abdullah S.A., Yahia A. Maher, Adel Fathi, Mohammed A.S. Abourehab, Mohammed Alarjah, Mohd S.A. Khan, and Saleh B. Al-Ghamdi. 2018. “Identification and Characterization of a Novel Antimicrobial Peptide Compound Produced by Bacillus Megaterium Strain Isolated from Oral Microflora.” Saudi Pharmaceutical Journal 26 (8): 1089–97. https://doi.org/10.1016/j.jsps.2018.05.019.
Awais, Muhammad, Aamer Ali Shah, Abdul Hameed, and Fariha Hasan. 2007. “Isolation, Identification and Optimization of Bacitracin Produced by Bacillus SP.” Pakistan Journal of Botany 39 (4): 1303–12.
Dahesihdewi, Andaru, Adhi Kristianto Sugianli, and Ida Parwati. 2019. “The Surveillance of Antibiotics Resistance in Indonesia: A Current Reports.” Bali Medical Journal 8 (2): 565. https://doi.org/10.15562/bmj.v8i2.1386.
Esikova, TZ, Yu Temirov, Sokolov, and Yu Alakhov. 2002. “Secondary Antimicrobial Metabolites Produced by Thermophilic Bacillus Spp. Strains VK2 and VK1.” Applied Biochemistru and Microbiology 38 (3): 226–31. https://doi.org/10.1023/A.
Fachrial, E., Harmileni, S. Anggraini, T.T. Nugroho, and Saryono. 2019. “Inulinase Activity of Thermophilic Bacteria Isolated from Hot Springs of Penen Village, North Sumatera, Indonesia.” In IOP Conference Series: Earth and Environmental Science. Vol. 406. https://doi.org/10.1088/1755-1315/406/1/012012.
Fachrial, Edy, Sari Anggraini, Harmileni, Titania T. Nugroho, and Saryono. 2019. “Isolation and Molecular Identification of Carbohydrase and Protease Producing Bacillus Subtilis JCM 1465 Isolated from Penen Hot Springs in North Sumatra, Indonesia.” Biodiversitas 20 (12): 3493–98. https://doi.org/10.13057/biodiv/d201205.
Fachrial, Edy, Raden Roro Jenny Satyo Putri, I. Nyoman Ehrich Lister, Sari Anggraini, Harmileni, Titania T. Nugroho, and Saryono. 2020. “Molecular Identification of Cellulase and Protease Producing Bacillus Tequilensis Utmsa14 Isolated from the Geothermal Hot Spring in Lau Sidebuk Debuk, North Sumatra, Indonesia.” Biodiversitas 21 (10): 4719–25. https://doi.org/10.13057/biodiv/d211035.
Khalil, Rowaida, Yasser Elbahloul, Fatima Djadouni, and Sanaa Omar. 2009. “Isolation and Partial Characterization of a Bacteriocin Produced by a Newly Isolated Bacillus Megaterium 19 Strain.” Pakistan Journal of Nutrition 8 (3): 242–50. https://doi.org/10.3923/pjn.2009.242.250.
Li, Hui, Lele Li, Yuanyuan Chi, Qingwu Tian, Tingting Zhou, Chunhua Han, Yuanqi Zhu, and Yusun Zhou. 2020. “Development of a Standardized Gram Stain Procedure for Bacteria and Inflammatory Cells Using an Automated Staining Instrument.” MicrobiologyOpen 9 (9): 1–10. https://doi.org/10.1002/mbo3.1099.
Malanicheva, I. A., D. G. Kozlov, I. G. Sumarukova, O. V. Efremenkova, V. A. Zenkova, G. S. Katrukha, M. I. Reznikova, O. D. Tarasova, S. P. Sineokii, and G. I. El’-Registan. 2012. “Antimicrobial Activity of Bacillus Megaterium Strains.” Microbiology 81 (2): 178–85. https://doi.org/10.1134/S0026261712020063.
MANE, ROHIT SHANKAR, and ASAMA NADAF. 2022. “Brief Review on Thermophilic Bacteria and Their Applications.” International Journal of Current Pharmaceutical Research 14 (1): 15–19. https://doi.org/10.22159/ijcpr.2022v14i1.44107.
Moshafi, Mohammad Hasan, Hamid Forootanfar, Alieh Ameri, Mojtaba Shakibaie, Gholamreza Dehghan-Noudeh, and Mojdeh Razavi. 2011. “Antimicrobial Activity of Bacillus Sp. Strain FAS1 Isolated from Soil.” Pakistan Journal of Pharmaceutical Sciences 24 (3): 269–75.
Muhammad, Syed Aun, Safia Ahmad, and Abdul Hameed. 2009. “Antibiotic Production by Thermophilic Bacillus Specie SAT-4.” Pakistan Journal of Pharmaceutical Sciences 22 (3): 339–45.
Nguyen, Tu H.K., and Le B. Thu. 2015. “Evaluation of Antimicrobial Activities of Bacillus Megaterium with a Third-Generation Cephalosporin (Ceftriaxone).” Journal of Applied Pharmaceutical Science 5 (9): 016–020. https://doi.org/10.7324/JAPS.2015.50903.
Octarya, Zona, Titania T. Nugroho, Yuana Nurulita, and Saryono. 2022. “Molecular Identification, GC-MS Analysis of Bioactive Compounds and Antimicrobial Activity of Thermophilic Bacteria Derived from West Sumatra Hot-Spring Indonesia.” HAYATI Journal of Biosciences 29 (4): 549–61. https://doi.org/10.4308/hjb.29.4.549-561.
Odonkor, Stephen T, and Addo Kennedy K. 2012. “Bacteria Resistance to Antibiotics: Recent Trends and Challenges.” International Journal of Biological & Medical Research 2 (4): 1204–10. papers3://publication/uuid/D08CCDB8-8E18-469F-87E3-2704311AC78B.
Pandey, Anita, Kusum Dhakar, Avinash Sharma, Payal Priti, Priyanka Sati, and Bhavesh Kumar. 2015. “Thermophilic Bacteria That Tolerate a Wide Temperature and PH Range Colonize the Soldhar (95 °C) and Ringigad (80 °C) Hot Springs of Uttarakhand, India.” Annals of Microbiology 65 (2): 809–16. https://doi.org/10.1007/s13213-014-0921-0.
Patel, Jayshree D, and Reza Fadaei. 2016. “Cost of Antibiotics in Society and Economic Approach.” Journal of Economics and Finance 7 (6): 17–20. https://doi.org/10.9790/5933-0706031720.
Prihanto, Asep Awaludin, Hidayatun Muyasyaroh, Abdul Aziz Jaziri, and Itorul Nada Umam. 2020. “Isolation and Molecular Characterization of Gelatinase-Producing Bacteria from Mangrove Sediment.” Biogenesis 8 (1): 10–14. https://doi.org/10.1063/5.0018150.
Saimin, Juminten, Hartati, Yenti Purnamasari, Sufiah Asri Mulyawati, Tien, and Pranita Aritrina. 2020. “Microbiological and Biochemical Contamination Analysis of Refilled Drinking-Water in Abeli, Kendari, Southeast Sulawesi.” Indonesian Biomedical Journal 12 (2): 124–29. https://doi.org/10.18585/inabj.v12i2.871.
STOICA, ROXANA-M?D?LINA, MI?U MOSCOVICI, CATERINA TOMULESCU, ANGELA C???RIC?, NARCISA B?BEANU, OVIDIU POPA, and HATICE AHU KAHRAMAN. 2019. “Antimicrobial Compounds of the Genus Bacillus: A Review.” Romanian Biotechnological Letters 24 (6): 1111–19. https://doi.org/10.25083/rbl/24.6/1111.1119.
Ulucay, Orhan, Arzu Gormez, and Cem Ozic. 2022. “Identification, Characterization and Hydrolase Producing Performance of Thermophilic Bacteria: Geothermal Hot Springs in the Eastern and Southeastern Anatolia Regions of Turkey.” Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology 115 (2): 253–70. https://doi.org/10.1007/s10482-021-01678-5.
Xie, Yudan, Qiuju Peng, Yuyu Ji, Ailin Xie, Long Yang, Shuzhen Mu, Zhu Li, et al. 2021. “Isolation and Identification of Antibacterial Bioactive Compounds From Bacillus Megaterium L2.” Frontiers in Microbiology 12 (March): 1–10. https://doi.org/10.3389/fmicb.2021.645484.
Yilmaz, Mirac, Haluk Soran, and Yavuz Beyatli. 2006. “Antimicrobial Activities of Some Bacillus Spp. Strains Isolated from the Soil.” Microbiological Research 161 (2): 127–31. https://doi.org/10.1016/j.micres.2005.07.001.

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