Burkholderia cepacia strain IrV1 multi-resistant to copper and dyes isolated from laboratory wastewater effluent

##plugins.themes.bootstrap3.article.sidebar##

PDF
DOI https://doi.org/10.13057/biodiv/d230544
Issue
Vol. 23 No. 5 (2022)
Section
Articles
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

##plugins.themes.bootstrap3.article.main##

WAHYU IRAWATI ♥
Department of Biology Education, Faculty of Education, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia
VALENTINE LINDARTO
Department of Natural Sciences, Dian Harapan Lippo Village High School. Jl. Imam Bonjol No. 201, Lippo Kawaraci, Tangerang 15811, Banten, Indonesia
REINHARD PINONTOAN
Department of Biology, Faculty of Science and Technology, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia
TRIWIBOWO YUWONO
Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada. Jl. Flora No. 1, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia
FEBRI MANGOMO MANGUNSONG
Department of Science Education, Faculty of Education, Dian Harapan Lippo Village High School. Jl. Imam Bonjol No. 201, Lippo Karawaci, Tangerang 15811, Banten, Indonesia
DESTYA WATY SILALAHI
Department of Biology Education, Faculty of Education, Universitas Pelita Harapan. Jl. M.H. Thamrin Boulevard 1100, Lippo Karawaci, Tangerang 15811, Banten, Indonesia

Abstract

Abstract. Irawati W, Lindarto V, Pinontoan R, Yuwono T, Mangunsong FM, Silalahi DW. 2022. Burkholderia cepacia strain IrV1 multi-resistant to copper and dyes isolated from laboratory wastewater effluent. Biodiversitas 23: 2614-2620. Copper and dye multi-resistant bacteria may be cultivated to increase the efficiency of waste bioremediation processes. Multi-resistant bacteria can be isolated from waste containing copper and dyes, including laboratory waste. The aims of this study were to: (i) isolate copper-resistant bacteria from wastewater, (ii) determine the multi-resistance of bacteria against copper and dyes, and (iii) observe the ability of bacteria to remove colors methylene blue, basic fuchsin, and Congo red. Selected bacterial isolates were identified based on the 16S rDNA gene. One highly copper resistant bacterial strain IrV, showed resistance to 7 mM CuSO4, 100 ppm methylene blue, 100 ppm basic fuchsin, and 100 ppm Congo red. Based on the 16S rDNA gene sequence, strain IrV belongs to Burkholderia cepacia with 99.86% similarity. The addition of copper on the medium resulted in colony color changes from light yellow to green indicated Cu binding within the cell. Moreover, a clear zone around the colony was observed, suggesting that the strain is capable of decolorizing methylene blue, Congo red, and basic fuchsin dyes. The results thus demonstrate that bacterial strain IrV is multi-resistant to copper and dyes.

##plugins.themes.bootstrap3.article.details##

References
Al-Sulami A, Jaafar R. 2015. Biosorption and Bioaccumulation of Some Heavy Metals by Deinococcus Radiodurans Isolated from Soil in Basra Governorate- Iraq. J Biotechnol Biomater 5 (2): 190.
Anonim. (2000). Health Effects of Excess Copper in Drinking Water. In: Copper in Drinking Water. National Academies Press, US.
Arifin A, Hayati Z, Jamil KF. 2016. Isolation and Identification of Bacteria At Clinical Microbiology Laboratory of Zainoel Abidin General Hospital Banda Aceh. JIMKI 1 (4): 1–8.
Barber RG, Grenier ZA, Burkhead, JL. 2021. Copper Toxicity Is Not Just Oxidative Damage: Zinc Systems and Insight from Wilson Disease. Biomedicines 9 (3): 316.
Bondarczuk K, Piotrowska-Seget Z. 2013. Molecular basis of active copper resistance mechanisms in Gram-negative bacteria. Cell Biol Toxicol 29 (6): 397–405.
Delahaut V, Raškovi? B, Salvado MS, Bervoets L, Blust R, de Boeck G. 2020. Toxicity and bioaccumulation of Cadmium, Copper and Zinc in a direct comparison at equitoxic concentrations in common carp (Cyprinus carpio) juveniles. PLoS ONE 15 (4): e0220485.
Dianrevy A. 2017. Penerapan Bakteri Indigenus Untuk Remediasi Limbah Cair Batik Pewarna Napthol Merah Dan Menurunkan Logam Cu (Tembaga). JUAJY: 1–16.
Diep P, Mahadevan R, Yakunin AF. 2018. Heavy Metal Removal by Bioaccumulation Using Genetically Engineered Microorganisms. Front Bioeng Biotechnol 6: 157.
Dupont CL, Grass G, Rensing C. 2011. Copper toxicity and the origin of bacterial resistance—new insights and applications. Metallomics 3 (11): 1109–18.
Dykhuizen D. 2005. Species Numbers in Bacteria. Proceedings of the California Academy of Sciences, 56-6-1: 62–71.
Espírito Santo C, Morais PV, Grass G. 2010. Isolation and Characterization of Bacteria Resistant to Metallic Copper Surfaces. Appl Environ Microbiol 76 (5): 1341–8.
Fazzo L, Minichilli F, Santoro M, Ceccarini A, della Seta M, Bianchi F, Comba P, Martuzzi M. 2017. Hazardous waste and health impact: a systematic review of the scientific literature. Environ Health 16 (1): 107.
Goodell B, Qian Y, Jellison J, Richard M. 2004. Decolorization and Degradation of Dyes with Mediated Fenton Reaction. Water Environ Res 76 (7): 2703–7.
Grey B, Steck TR. 2001. Concentrations of Copper Thought To Be Toxic to Escherichia coli Can Induce the Viable but Nonculturable Condition. Appl Environ Microbiol 67 (11): 5325–7.
Hassan MM, Carr, CM. 2018. A critical review on recent advancements of the removal of reactive dyes from dyehouse effluent by ion-exchange adsorbents. Chemosphere 209: 201–19.
Higgins S, Gualdi S, Pinto?Carbó M, Eberl L. 2020. Copper resistance genes of Burkholderia cenocepacia H111 identified by transposon sequencing. Environ Microbiol Rep 12 (2): 241–9.
Ibrahim M, Wang F, Lou M, Xie G, Li B, Bo Z, Zhang G, Liu H, Wareth A. 2011. Copper as an antibacterial agent for human pathogenic multidrug resistant Burkholderia cepacia complex bacteria. J Biosci Bioeng 112 (6): 570–6.
Imran M, Crowley DE, Khalid A, Hussain S, Mumtaz MW, Arshad M. 2015. Microbial biotechnology for decolorization of textile wastewaters. Rev Environ Sci Biotechnol 14 (1): 73–92.
Irawati W, Djojo ES, Kusumawati L, Yuwono T, Pinontoan R. 2021. Optimizing Bioremediation: Elucidating Copper Accumulation Mechanisms of Acinetobacter sp. IrC2 Isolated From an Industrial Waste Treatment Center. Front Microbiol 12: 713812.
Irawati W, Pinontoan R, Yuwono T. 2020. Indigenous copper resistant bacteria isolated from activated sludge of water treatment plant in Surabaya, Indonesia. Biodiversitas 21 (11): 5077–84.
Irawati W, Pinontoan R, Mouretta B, Yuwono T. 2021. The potential of copper-resistant bacteria Acinetobacter sp. strain CN5 in decolorizing dyes. Biodiversitas 23 (2): 679-685.
Irawati W, Sopiah N, Sulista S. 2017. Heavy Metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia. Biodiversitas 16: 1481–6.
Islam T, Rahman MdS, Hussain MdS. 2017. Heavy Metal Tolerance Pattern of Textile Dye Degrading Native Bacteria: A Bioremediation Viewpoint. Ann Med Red 7: 67–73.
Isola OB, Olatunji EO. 2016. Isolation and Identification of Microorganisms from Surfaces in Science Laboratories. SAU J Sci Technol 1 (1): 80–7.
Kim K, Lee K, So S, Cho S, Lee M, You K, Moon J, Song T. 2018. Fenton-Like Reaction between Copper Ions and Hydrogen Peroxide for High Removal Rate of Tungsten in Chemical Mechanical Planarization. ECS J Solid State Sci Technol 7 (3): 91–5.
Laszlo JA. 2000. Regeneration of Azo-Dye-Saturated Cellulosic Anion Exchange Resin by Burkholderia cepacia Anaerobic Dye Reduction. Environ Sci Technol 34 (1): 167–72.
Lellis B, Fávaro-Polonio CZ, Pamphile JA, Polonio JC. 2019. Effects of textile dyes on health and the environment and bioremediation potential of living organisms. Biotechnol Res Innov 3 (2): 275–90.
Lobo V, Patil A, Phatak A, Chandra N. 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4 (8): 118–26.
Nurlaila I, Irawati W, Purwandari K, Pardamean B. 2020. K-Means Clustering Model to Discriminate Copper-Resistant Bacteria as Bioremediation Agents. Procedia Comput Sci 179: 804–12.
RC, Dupont CL. 2020. Genomic and phenotypic characterization of Burkholderia isolates from the potable water system of the International Space Station. PLoS ONE 15 (2): e0227152.
Pohanish RP. 2017. Sittig’s handbook of toxic hazardous chemicals and carcinogens. Willian Andrew Publishing, New York.
Purchase R. 2013. The Link between Copper and Wilson’s Disease. Sci Prog 96 (3): 213–23.
Rehman K, Fatima F, Waheed I, Akash, MSH. 2018. Prevalence of exposure of heavy metals and their impact on health consequences. J Cell Biochem 119 (1): 157–84.
Ren S, Guo J, Zeng G, Sun G. 2006. Decolorization of triphenylmethane, azo, and anthraquinone dyes by a newly isolated Aeromonas hydrophila strain. Appl Microbiol Biotechnol 72 (6): 1316–21.
Sandhya S. 2010. Biodegradation of Azo Dyes Under Anaerobic Condition: Role of Azoreductase. In: The Handbook of Environmental Chemistry. Springer, Switzerland.
Saratale RG, Saratale GD, Chang JS, Govindwar SP. 2011. Bacterial decolorization and degradation of azo dyes: A review. J Taiwan Inst Chem Eng 42 (1): 138–57.
Shamim S. 2018. Biosorption of Heavy Metals. In Biosorption. InTech, London.
Sivarajasekar N, Baskar R. 2014. Adsorption of basic red 9 on activated waste Gossypium hirsutum seeds: Process modeling, analysis and optimization using statistical design. J Ind Eng Chem 20 (5): 2699–709.
Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. 2012. Heavy Metal Toxicity and the Environment. Exp Suppl 101: 133–64.
Torbeck L, Raccasi D, Guilfoyle DE, Friedman RL, Hussong D. 2011. Burkholderia cepacia: This Decision Is Overdue. PDA J Pharm Sci Technol 65 (5): 535–43.
Victor H, Ganda V, Kiranadi B, Pinontoan, R. 2020. Metabolite Identification from Biodegradation of Congo Red by Pichia sp. KnE Life Sci 5 (2): 102–10.

Most read articles by the same author(s)

1 2 > >>