Short Communication: Analysis of the chromosome numbers of Zinnia elegans Jacq. in single, double, and pom-pom flowers




Abstract. Saifudin, Shafira S, Dwiranti A, Salamah A. 2021. Short Communication: Analysis of the chromosome numbers of Zinnia elegans Jacq. in single, double, and pom-pom flowers. Biodiversitas 22: 2771-2777. Zinnia elegans Jacq. is highly valued as an ornamental plant with a variety of flower colors, sizes, and shapes. Polyploidization has been reported in Z. elegans with pom-pom flowers, nevertheless, the variation in chromosome numbers of various flower shapes has yet to be investigated. This study aimed to analyze the chromosome numbers of Z. elegans Jacq. cultivar “California Giant,” “Lilliput,” and “Cactus Flowered Mix” with single, double, and pom-pom flowers to determine their variations and identify the morphology of the flowers. Chromosomes were prepared using the squashing method, and images were analyzed using the Chromosome Image Analyzing System (CHIAS) IV. The minimum of 5 slides was prepared for each flower type from each cultivar. The results show that the three cultivars are diploid plants (2n = 24) with varying flower morphology. The single and double flowers of Z. elegans “Lilliput” and the single flower of “Cactus Flowered Mix” showed no variation in chromosome numbers. In contrast, chromosome number variation was found in the pom-pom flower of Z. elegans “California Giant” (2n = 22, 24, 48) and the double flower of “Cactus Flowered Mix” (2n = 9, 13, 15, 24). Two cultivars, Z. elegans “California Giant” and Z. elegans “Cactus Flowered Mix,” were successfully analyzed using CHIAS IV. Statistical analysis using a t-test (? = 0.05) showed that the total chromosome length of Z. elegans “California Giant” (2n = 24) was significantly greater than that of Z. elegans “Cactus Flowered Mix” (2n = 24). Chromosome satellites were found in both cultivars.


Anantasaran J. 2007. Cytogenetic and molecular characterization and in vitro culture of Zinnia species (Thesis). Thailand: Universitas Prince of Songkla.
Anderson NO. 2007. Flower breeding and genetics: issues, challenges and opportunities for the 21st century. California: Springer Science & Business Media.
Aziz IR. 2019. Kromosom tumbuhan sebagai marka genetik. Jurnal Teknosains 13(2): 125–131. DOI: 10.24252/teknosains.v13i2.9638
Azizi N, Sheidai M, Mozaffarian V, Nourmohammadi Z. 2014. Karyotype and genome size analyses in species of Helichrysum (Asteraceae). Acta Botanica Brasilica 28(3): 367–375.DOI: 10.1590/0102-33062014abb3136
Bhat TA, Wani AA. 2017. Chromosome structure and aberrations. India: Springer Nature.
Doyle, Coate. 2019. Polyploidy, the nucleotype, and novelty: the impact of genome doubling on the biology of the cell. International Journal of Plant Sciences 180(1): 1–52. DOI: 10.1086/700636
Du Y, Bi Y, Zhang M, Yang F, Jia G, Zhang X. 2017. Genome size diversity in Lilium (liliaceae) is correlated with karyotype and environmental traits. Frontiers Plant Science 8: 1–11.DOI: 10.3389/fpls.2017.01303
El-Shehawi AM, Elseehy MM. 2017. Genome size and chromosome number relationship contradicts the principle of darwinian evolution from common ancestor. Journal of Phylogenetics & Evolutionary Biology 5: 1–9. DOI: 10.4172/2329-9002.1000179
Fruth RL. 1991. Fertility and chromosome number in interspecific impatiens hybrids (Thesis). Iowa: Iowa State University.
Gupta PK, Koak R. 1976. Induced autotetraploidy in Zinnia elegans Jacq. Cytologia 41: 187–191. DOI: 10.1508/cytologia.41.18
Irawan, B. 2019. Genetika molekuler. Surabaya: Airlangga University Press.
Kato S, Ohmido N, Hara M, Kataoka R, Fukui K. 2009. Image analysis of small plant chromosomes by using an improved system, CHIAS IV. Chromosome Science 12: 43–50.
Kato S. 2015. Studies on the development of image analysis for plant research (Thesis). Japan: Kobe University.
Levan A, Fredga K, Sandberg AA. 1964. Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.
Misra RL, Misra S. 2017. Commercial ornamental crops: cut flowers. London: Kruger Brentt Publishers UK Limited.
Purwantoro A, Jamhari. 2017. Uji preferensi konsumen terhadap karakter bunga kembang kertas (Zinnia elegans Jacq.). Agroista: Jurnal Agroteknologi 1(1): 20–31.
Prakash NS, Lakshmi N, Harini I. 1988. A note on spontaneous mixoploid in Capsicum. Current Science 57(8): 435–436.
Puspita A, Setiawan AB, Purwantoro A, Sulistyaningsih E. 2020. Identifikasi kromosom homolog melalui deteksi nucleolus organizer regions dengan pewarnaan AgNO3 pada tanaman bawang merah. Bioteknologi & Biosains Indonesia 7(1): 9–17. DOI: 10.29122/jbbi.v7i1.3693
Ranjbar M, Karamian R, Nouri S. 2011. Diploid-tetraploid mixoploidy in a new species of Astragalus (Fabaceae) from Iran. Annales Botanici Fennici
: 343–351. DOI: 10.5735/085.048.0406
Salamah A, Oktarina R, Ambarwati EA, Putri DF, Dwiranti A, Andayani N. 2018. Chromosome numbers of some asteraceae species from Universitas Indonesia campus, Depok, Indonesia. Biodiversitas 19(6): 2079–2087. DOI: 10.13057/biodiv/d190613
Singh RJ. 2003. Plant cytogenetics second edition. USA: CRC Press LLC.
Singh RJ. 2018. Practical Manual on Plant Cytogenetics. Boca Raton: CRC Press.
Stansfield WD. 1991. Theory and problems of genetics third edition. USA: The McGraw-HiM Companies.
Torres AM. 1963. Taxonomy of Zinnia. Brittonia 15(1): 1–25. DOI: 10.2307/2805035
Zhang Y, Zhu ML, Dai SL. 2013. Analysis of karyotype diversity of 40 Chinese chrysanthemum cultivars. Journal of Systematics and Evolution 51(3): 335–352. DOI: 10.1111/J.1759-6831.2012.00235.X

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