Short Communication: Phytochemical diversity and bioactivity of Malaysian Premna cordifolia (Lamiaceae)




Abstract. Daud D, Dewa MSAM, Mahbob ENM, Razak WRWA. 2021. Short Communication: Phytochemical diversity and bioactivity of Malaysian Premna cordifolia (Lamiaceae). Biodiversitas 22: 3245-3248. The aims of the present study are to determine the chelation potential and protective effect of Premna cordifolia leaves ethanolic extract in lead intoxicated mice. Twenty-four male mice were divided into four groups with six animals each. Group 1 was healthy mice and received 2 ml/kg bwt distilled water. Group 2 was healthy mice and supplemented with 200 mg/kg bwt P. cordifolia leaves ethanolic extract. Group 3 was lead intoxicated mice and received 2 ml/kg bwt distilled water. Group 4 was lead intoxicated mice and supplemented with 200 mg/kg bwt P. cordifolia leaves ethanolic extract. All treatments were given for 14 days. Chelation activity was determined by measuring the concentrations of lead in the liver, kidney and testis by using atomic absorption spectroscopy. Sperm quality was tested according to World Health Organization manual and testosterone was estimated by using ELISA kit. Spectroscopic data showed that P. cordifolia leaves ethanolic extract was able to lower lead concentrations in liver, kidney and testis of lead intoxicated mice. Following a decrease in lead concentrations, sperm quality and testosterone levels were restored in lead intoxicated mice supplemented with P. cordifolia leaves ethanolic extract. In conclusion, P. cordifolia leaves ethanolic extract has the potential to be developed as an alternative remedy for treating lead poisoning effects.


Adhikari A, Darbar S, Chatterjee T, Das M, Polley N, Bhattacharyya M, Bhattarcharyya S, Pal D, Pal SK. 2018. Spectroscopic studies on dual role of natural flavonoids in detoxification of lead poisoning: Bench to bedside trial. ACS Omega 3: 15975-15987.
Amoriwicz R. 2007. Tannins: The new natural antioxidants? Eur J Lipid Sci Technol 109: 549-551.
Daud D, Md Nawi M, Ismail A, Tawang A. 2015. The effects of Eucheuma cottonii on sperm quality and tissue lead level in rats exposed to lead nitrate. Biotechnology An Indian J 11(4): 138-143.
El-Khadragy M, Al-Megrin WA, Al-Sadhan NA, Metwally DM, El-Hennamy RE, Salem FEH, Kassab RB, Abdel-Moneim AE. 2020. Impact of coenzyme Q10 administration on lead acetate-induced testicular damaged in rats. Oxid Med Cell Longev doi.10.1155/2020/4981386.
Flora SJS. 2009. Structural, chemical and biological aspects of antioxidants for strategies against metal and metalloid exposure. Oxid Med Cell Longev 2(4): 191-206.
Flora SJS, Pachauri V. 2010. Chelation in metal intoxication. Int J Environ Res Public Health 7(7): 2745-2788.
Harborne JB. 1973. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall, London.
Hui CK, Majid NI, Mohd Zainol MK, Mohamad H, Mohd Zin Z. 2018. Preliminary phytochemical screening and effect of hot water extraction conditions on phenolic contents and antioxidant capacities of Morinda citrifolia leaf. Malays Appl Biol 47(4): 13-24.
Kunnambath PM, Thirumalaisamy S. 2015. Characterization and utilization of tannin extract for the selective adsorption of Ni (II) ions from water. J Chemistry doi: 10.1155/2015/498359.
Leon OLL, Pacheco JMS. 2020. Effects of lead on reproductive health. IntechOpen doi:10.5772/intechopen.91992.
Lotfi-Ghahramanloo M, Baghshani H. 2016. Ameliorative effects of caffeic acid on lead accumulation and oxidative stress in lead-exposed mice. Zahedan J Res Med Sci 18(5): e6674.
Maddu N. 2019. Diseases related to types of free radicals. IntechOpen doi: 10.5772/intechopen.82879.
Md Jani MH, Daud D, Sharkawi N, Hashim H, Ismail NH, Salleh A, Hashim N. 2018. The effect of Zingiber zerumbet ethanol extract (ZZREE) on testosterone level and testes histoarchitecture in high fat diet-induced obese rats. Malays Appl Biol 47(5): 181-187.
Mehrandish R, Rahimian A, Shahriary A. 2019. Heavy metals detoxification: A review of herbal compounds for chelation therapy in heavy metals toxicity. J Herbmed Pharmacol 8(2): 69-77.
Onuegbu GC, Eze FI, Ayogu PC, Agbo MO, Ezema CG, Ezema BE. 2019. Phytochemicaland in vitro antimicrobial properties of Premna hispida (Verbenaceae) Linn. IOSR J Pharm Biol Sci 14(5): 64-74.
Sani A, Abdullahi IL. 2017. Evaluation of some heavy metals concentration in body fluids of metal workers in Kano metropolis, Nigeria. Toxicol Rep 4: 72-76.
Sharkawi NA, Ismail NH, Md Jani NH, Daud D, Hashim N, Salleh A, Hashim H. 2019. Curative potential of Zingiber zerumbet on paracetamol-induced hepatoxicity. Malays Appl Biol 48(3): 11-17.
Sofowora A. 1993. Medicinal plants and traditional medicine in Africa. Spectrum Books, Ibadan.
Soleimanzadeh A, Kian M, Moradi S, Mahmoudi S. 2019. Carob (Ceratonia siliqua L) fruit hydro-alcoholic extract alleviates reproductive toxicity of lead in male mice: Evidence on sperm parameters, sex hormones, oxidative stress biomarkers and expression of Nrf2 and iNOS. Avicenna J Phytomed 10(1): 35-49.
Vaclavikova R, Kondrova E, Ehrlichova DM, Boumendjel A, Kovar J, Stopka P. 2008. The effect of flavonoid derivatives on doxorubicin transport and metabolism. Bioorg Med Chem 16(4): 2034-2042.
Wagner H. 1993. Pharmazeutische Biology. Gustav Fisher Vweleg, Germany.
World Health Organization. 1999. WHO Laboratory Manual for The Examination of Human Semen and Sperm Cervical Mucus Interaction. Cambridge University Press, Cambridge.