Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Ramona Y, Darmayasa IBG, Kusuma AANN, Line MA. 2021. Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens. Biodiversitas 22: 298-303. This study investigated the inhibitory potential of diversity of antagonist bacteria residing in the rhizosphere zone and mature compost to counter fungal plant pathogens. Soils collected from rhizosphere of lettuce farms in Bali-Indonesia and Tasmania-Australia, mature compost, commercial biocontrol (Dipel®), and laboratory contaminants with significant inhibition against tested fungal pathogens were used as sources of antagonist bacteria. These antagonists were isolated by applying dilution and spread method on trypticase soya agar (TSA) or potato dextrose agar (PDA), and their ability to inhibit Sclerotinia minor, Sclerotinia sclerotiorum, Fusarium spp., and Rhizoctonia solani was assessed in dual culture assays. The results showed that 67 out of more than 100 isolates had antagonistic activity in vitro against at least one of tested fungal pathogens. In the preliminary identification, Bacillus spp. or Pseudomonas spp. were found to be pre-dominant isolates. Following screening studies in a non-replicated glasshouse experiment against S. minor and S. sclerotiorum, 8 of the most promising isolates were further identified using molecular methods based on their 16s rDNA sequences aligned with those deposited at the GeneBank. These 8 isolates were identified as Pseudomonas corrugata, Bacillus megaterium, Bacillus polymyxa, Bacillus mojavensis, Bacillus pumilus, Bacillus thuringiensis, Exiguobacterium acetylicum, and Chryseobacterium indologenes.
##plugins.themes.bootstrap3.article.details##
References
Anith KN, Radhakrishnan NV, and Manomohandas TP. 2003. Screening of antagonistic bacteria for biological control of nursery wilt of black pepper (Piper nigrum). Microbiol. Res. 158: 91–97. DOI: http://www.urbanfischer.de/journals/microbiolres.
Catara V. 2007. Pseudomonas corrugate: Plant pathogen and/or biological resource? Molecular plant Pathology 8(3): 233 - 244. DOI: https://doi.org/10.1111/j.1364-3703.2007.00391.x.
Ciancio A, Pieterse CMJ and Mercado-Blanco J. 2016. Editorial: Harnessing useful rhizosphere microorganisms for pathogen and pest biocontrol. Front. Microbiol. 7:1620. doi: 10.3389/fmicb.2016.01620
da Costa Capizzani CP, Cac¸adora NC, Marquesb EA, Levyc CE, Tonania L, Torresd LAGMM, and da Costa Darinia,A L. 2018. A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis. Brazilian Journal of Microbiology 4 9: 422 – 428 .
Fernandes FL, Bacci L, and Fernandes MS. 2010. Impact and selectivity of insecticides to predators and parasitoids. EntomoBrasilis 3 (1): 1 - 10.
Gehan AE, Samia HAZ, Ehab ADS, Mehreshan TEL, and Abdel-Wahab AFM. 2018. In vitro suppressive effect of agriculture residues and municipal solid wastes compost tea on some phytopathogenic fungi. J. Sci. Res. Sci. 35: 181 – 202.
Gómez Expósito R, Postma J, Raaijmakers JM and De Bruijn I. 2015. Diversity and activity of Lysobacter species from disease suppressive soils. Front. Microbiol. 6:1243. Doi: 10.3389/fmicb.2015.01243.
Ghorbanpour M, Omidvari M, Abbaszadeh-Dahaji P, Omidvar, R, and Kariman K. 2018. Mechanisms underlying the protective effects of beneficial fungi against plant diseases. Biological Control. 117: 147-157. DOI: https://doi.org/10.1016/j.biocontrol.2017.11.006.
Heidarzadeh N, Beghaee-Ravari S. 2016. Application of Bacillus pumilus as a potential biocontrol agent of Fusarium wilt of tomato. Archives of Phytopathology and Plant Protection 48(13-16): 841-849. DOI: https://doi.org/10.1080/03235408.2016.1140611.
Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley, and S.T. Williams. 1994. Bergey’s manual of determinative bacteriology. 9th Ed. Lippincott Williams and Wilkins. Philadelphia.
Inayati, A, Sulistyowati L, Aini LQ, and Yusnawan E. 2020. Mycoparasitic activity of indigenous Trichoderma virens strains against mungbean soil borne pathogen Rhizoctonia solani: Hyperparasite and hydrolytic enzyme production. AGRIVITA Journal of Agricultural Science 42(2): 229–242. DOI: https://doi.org/10.17503/agrivita.v0i0.2514.
Kim KH, Kabir, E, and Jahan SA. 2017. Exposure to pesticides and the associated human health effects. Science of the Total Environment 575: 525–535. DOI: http://dx.doi.org/10.1016/j.scitotenv.2016.09.009.
Kunova, A., Bonaldi, M., Saracchi, M., Pizzatti, C., Chen, X., and Cortesi, P. 2016. Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth. BMC Microbiology.16:272. DOI: 10.1186/s12866-016-0886-1.
Larkin RP, Fravel DR. 1998. Efficacy of various fungal and bacterial biocontrol organisms for control of Fusarium wilt of tomato. Plant Disease 82 (9): 1022 – 1028.
Lucas JA, Hawkin NJ, and Fraaije BA. 2015. The evolution of fungicide resistance. Adv. Appl. Microbiol. 90: 29–92. DOI: 10.1016/bs.aambs.2014.09.001
Malleswari D. 2014. In vitro antagonistic activity of diverse bacterial isolates against Macrophomina phaseolina (Tassi) Goid. Int.J.Curr.Microbiol.App.Sci. 3(5): 755-763.
Meena RS, Kumar S, Datta R, Lal R, Vijayakumar V, Brtnicky M, Sharma MP, Yadav G S, Jhariya MK, Jangir C K, Pathan SI, Dokulilova T, Pecina, V, and Marfo TD. 2020. Impact of agrochemicals on soil microbiota and management: A Review. Land. 9 (34): 1 – 21. DOI: 10.3390/land9020034.
Martínez-Viveros O, Jorquera MA, Crowley, DE, Gajardo G, and Mora ML. 2010. Mechanism and practical considerations involved in plant growth promotion by rhizobacteria. J. Soil Sci. Plant Nutr. 10 (3): 293 – 319.
Mauti EM, Mauti GO, Ouno GA, Mabeya B M, and Kiprono SJ. 2013. Molecular identification of soil bacteria by 16srDNA sequence. Journal of Natural Sciences Research 3(14): 51 – 58.
Nicolopoulou-Stamati P, Maipas S, Kotampasi C, Stamatis P, and Hens L. 2016. Chemical pesticides and human health: The urgent need for a new concept in agriculture. Front. Public Health 4:148. doi: 10.3389/fpubh.2016.00148.
Ntushelo K, Ledwaba LK, Rauwane ME, Adebo, OA, and Njobeh PB. 2019. The mode of action of Bacillus species against Fusarium graminearum, tools for investigation, and future prospects. Toxin 11(606): 1 – 14. DOI:10.3390/toxins11100606
Ramona Y. 2003 Assessment of some antagonists to fungal plant pathogens and development of methods for their large scale cultivation. Ph.D Thesis. School of Agricultural Science, The University of Tasmania Australia.
Ramona Y, Sujaya IN, Nocianitri KA, Aryantha WR, Cintyadewi R, Maha Uni IAS, and Nursini NM. 2015. Characterization and molecular identification of Lactobacillus spp. isolated from feces of healthy infants for local probiotic development in Bali. Proceeding of International Conference on Life Sciences and Biotechnology. Jember University-East Java. Jember, 28 – 29 September 2015. [Indonesia].
Shelby N, Duncan RP, Van der Putten WH, McGinn KJ, Weser C, and Hulme PE. 2016. Plant mutualisms with rhizosphere microbiota in introduced versus native ranges. Journal of Ecology 104: 1259–1270. DOI: 10.1111/1365-2745.12609.
Suthar KP, Patel RM, Singh D, Parekh VB, Khunt MD, Ahmad T. 2017. Isolation and characterization of antagonist bacteria from chickpea rhizosphere against Fusarium oxysporum f. sp. ciceris. Journal of Pure and Applied Microbiology 11(1): 371 – 378.
Wang, M, Geng, L, Sun X, Shu C, Song F, and Zhang J. 2020. Screening of Bacillus thuringiensis strains to identify new potential biocontrol agents against Sclerotinia sclerotiorum and Plutella xylostella in Brassica campestris L. Biological Control 145. DOI: https://doi.org/10.1016/j.biocontrol.2020.104262.
Weller DM. 2007. Pseudomonas biocontrol agents of soilborne pathogens: Looking back over 30 years. Phytopathology 97(2): 251 – 256.
Wulansari N T, Ramona Y, and Proborini MW. 2015. Identification of Xanthomonas campestris isolated from rhizosphere zone of broccoli farm at Kembang Merta village, Tabanan-Bali. Jurnal Metamorfosa 2(1): 29 – 33.
Zheng XY, Sinclair JB. 2000. The effects of traits of Bacillus megaterium on seed and root colonization and their correlation withthe suppression of Rhizoctonia root rot of soybean. Biocontrol 45: 223 – 243.
Catara V. 2007. Pseudomonas corrugate: Plant pathogen and/or biological resource? Molecular plant Pathology 8(3): 233 - 244. DOI: https://doi.org/10.1111/j.1364-3703.2007.00391.x.
Ciancio A, Pieterse CMJ and Mercado-Blanco J. 2016. Editorial: Harnessing useful rhizosphere microorganisms for pathogen and pest biocontrol. Front. Microbiol. 7:1620. doi: 10.3389/fmicb.2016.01620
da Costa Capizzani CP, Cac¸adora NC, Marquesb EA, Levyc CE, Tonania L, Torresd LAGMM, and da Costa Darinia,A L. 2018. A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis. Brazilian Journal of Microbiology 4 9: 422 – 428 .
Fernandes FL, Bacci L, and Fernandes MS. 2010. Impact and selectivity of insecticides to predators and parasitoids. EntomoBrasilis 3 (1): 1 - 10.
Gehan AE, Samia HAZ, Ehab ADS, Mehreshan TEL, and Abdel-Wahab AFM. 2018. In vitro suppressive effect of agriculture residues and municipal solid wastes compost tea on some phytopathogenic fungi. J. Sci. Res. Sci. 35: 181 – 202.
Gómez Expósito R, Postma J, Raaijmakers JM and De Bruijn I. 2015. Diversity and activity of Lysobacter species from disease suppressive soils. Front. Microbiol. 6:1243. Doi: 10.3389/fmicb.2015.01243.
Ghorbanpour M, Omidvari M, Abbaszadeh-Dahaji P, Omidvar, R, and Kariman K. 2018. Mechanisms underlying the protective effects of beneficial fungi against plant diseases. Biological Control. 117: 147-157. DOI: https://doi.org/10.1016/j.biocontrol.2017.11.006.
Heidarzadeh N, Beghaee-Ravari S. 2016. Application of Bacillus pumilus as a potential biocontrol agent of Fusarium wilt of tomato. Archives of Phytopathology and Plant Protection 48(13-16): 841-849. DOI: https://doi.org/10.1080/03235408.2016.1140611.
Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley, and S.T. Williams. 1994. Bergey’s manual of determinative bacteriology. 9th Ed. Lippincott Williams and Wilkins. Philadelphia.
Inayati, A, Sulistyowati L, Aini LQ, and Yusnawan E. 2020. Mycoparasitic activity of indigenous Trichoderma virens strains against mungbean soil borne pathogen Rhizoctonia solani: Hyperparasite and hydrolytic enzyme production. AGRIVITA Journal of Agricultural Science 42(2): 229–242. DOI: https://doi.org/10.17503/agrivita.v0i0.2514.
Kim KH, Kabir, E, and Jahan SA. 2017. Exposure to pesticides and the associated human health effects. Science of the Total Environment 575: 525–535. DOI: http://dx.doi.org/10.1016/j.scitotenv.2016.09.009.
Kunova, A., Bonaldi, M., Saracchi, M., Pizzatti, C., Chen, X., and Cortesi, P. 2016. Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth. BMC Microbiology.16:272. DOI: 10.1186/s12866-016-0886-1.
Larkin RP, Fravel DR. 1998. Efficacy of various fungal and bacterial biocontrol organisms for control of Fusarium wilt of tomato. Plant Disease 82 (9): 1022 – 1028.
Lucas JA, Hawkin NJ, and Fraaije BA. 2015. The evolution of fungicide resistance. Adv. Appl. Microbiol. 90: 29–92. DOI: 10.1016/bs.aambs.2014.09.001
Malleswari D. 2014. In vitro antagonistic activity of diverse bacterial isolates against Macrophomina phaseolina (Tassi) Goid. Int.J.Curr.Microbiol.App.Sci. 3(5): 755-763.
Meena RS, Kumar S, Datta R, Lal R, Vijayakumar V, Brtnicky M, Sharma MP, Yadav G S, Jhariya MK, Jangir C K, Pathan SI, Dokulilova T, Pecina, V, and Marfo TD. 2020. Impact of agrochemicals on soil microbiota and management: A Review. Land. 9 (34): 1 – 21. DOI: 10.3390/land9020034.
Martínez-Viveros O, Jorquera MA, Crowley, DE, Gajardo G, and Mora ML. 2010. Mechanism and practical considerations involved in plant growth promotion by rhizobacteria. J. Soil Sci. Plant Nutr. 10 (3): 293 – 319.
Mauti EM, Mauti GO, Ouno GA, Mabeya B M, and Kiprono SJ. 2013. Molecular identification of soil bacteria by 16srDNA sequence. Journal of Natural Sciences Research 3(14): 51 – 58.
Nicolopoulou-Stamati P, Maipas S, Kotampasi C, Stamatis P, and Hens L. 2016. Chemical pesticides and human health: The urgent need for a new concept in agriculture. Front. Public Health 4:148. doi: 10.3389/fpubh.2016.00148.
Ntushelo K, Ledwaba LK, Rauwane ME, Adebo, OA, and Njobeh PB. 2019. The mode of action of Bacillus species against Fusarium graminearum, tools for investigation, and future prospects. Toxin 11(606): 1 – 14. DOI:10.3390/toxins11100606
Ramona Y. 2003 Assessment of some antagonists to fungal plant pathogens and development of methods for their large scale cultivation. Ph.D Thesis. School of Agricultural Science, The University of Tasmania Australia.
Ramona Y, Sujaya IN, Nocianitri KA, Aryantha WR, Cintyadewi R, Maha Uni IAS, and Nursini NM. 2015. Characterization and molecular identification of Lactobacillus spp. isolated from feces of healthy infants for local probiotic development in Bali. Proceeding of International Conference on Life Sciences and Biotechnology. Jember University-East Java. Jember, 28 – 29 September 2015. [Indonesia].
Shelby N, Duncan RP, Van der Putten WH, McGinn KJ, Weser C, and Hulme PE. 2016. Plant mutualisms with rhizosphere microbiota in introduced versus native ranges. Journal of Ecology 104: 1259–1270. DOI: 10.1111/1365-2745.12609.
Suthar KP, Patel RM, Singh D, Parekh VB, Khunt MD, Ahmad T. 2017. Isolation and characterization of antagonist bacteria from chickpea rhizosphere against Fusarium oxysporum f. sp. ciceris. Journal of Pure and Applied Microbiology 11(1): 371 – 378.
Wang, M, Geng, L, Sun X, Shu C, Song F, and Zhang J. 2020. Screening of Bacillus thuringiensis strains to identify new potential biocontrol agents against Sclerotinia sclerotiorum and Plutella xylostella in Brassica campestris L. Biological Control 145. DOI: https://doi.org/10.1016/j.biocontrol.2020.104262.
Weller DM. 2007. Pseudomonas biocontrol agents of soilborne pathogens: Looking back over 30 years. Phytopathology 97(2): 251 – 256.
Wulansari N T, Ramona Y, and Proborini MW. 2015. Identification of Xanthomonas campestris isolated from rhizosphere zone of broccoli farm at Kembang Merta village, Tabanan-Bali. Jurnal Metamorfosa 2(1): 29 – 33.
Zheng XY, Sinclair JB. 2000. The effects of traits of Bacillus megaterium on seed and root colonization and their correlation withthe suppression of Rhizoctonia root rot of soybean. Biocontrol 45: 223 – 243.
Most read articles by the same author(s)
- YAN RAMONA, IDA BAGUS GEDE DARMAYASA, MARTIN A. LINE, Biological control of Sclerotinia minor attack on pyrethrum plants by Trichoderma harzianum in glasshouse experiment , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 6 (2022)
- YENNI CIAWI, FAINMARINAT SELVIANI INABUY, NI MADE TERIYANI, YAN RAMONA, Potential of sodium dichromate and sodium silicate to control in vitro growth of Bacillus cereus, a metal corrosion-causing bacterium , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 3 (2023)