Identification and diversity analysis of cassiicolin-encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia
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Abstract. Oktavia F. 2020. Identification and diversity analysis of cassiicolin encoding gene of Corynespora cassiicola isolates from rubber tree in Indonesia. Biodiversitas 21: 3499-3507. Cassiicolin is an important effector of virulence process on the Corynespora cassiicola, plant-pathogen that causes Corynespora leaf fall disease in rubber tree. The study aimed to identify and analyze the genetic diversity of isolates from rubber plants in Indonesian based on cassiicolin encoding gene (Cas gene). Identification of Cas genes was performed using 12 pairs of primers on 23 isolates from rubber host clones. Only three pairs of primers were able to amplify the analyzed DNA, namely CasF20/CasR28, CasF19/CasR26, and CasF4/R16 which produced DNA fragments of 760 bp, 550 bp and 210 bp respectively in this study. BLAST analysis showed that 19 isolates carried a Cas5 gene. Three of the 19 DNA samples also contained a putative Cas3 or Cas4 gene. Four other isolates had no detectable Cas gene (Cas0). The genetic diversity based on the Cas gene showed that the isolates were grouped into three major clusters. ome isolates had high genetic similarity with outgroup isolates from China. There was not clear link between Cas gene with virulence profiles. The cassiicolin encoding gene information is useful for predicting the virulence of C. cassiicola isolates from Indonesia rubber plantation, so that the disease severity spreading in rubber plants can be early anticipated.
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References
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de Lamotte F, Duviau MP, Sanier C, Thai R, Poncet J, Bieysse D, Breton F, Pujade-Renaud V. 2007. Purification and characterization of Cassiicollin toxin produced by Corynespora cassiicola causal agent of the leaf fall disease of rubber tree. J Chrom 849: 357-362.
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Ogbebor ON. 2010. The status of three common leaf disease of Para rubber in Nigeria. JAnimal Plant Sci 6(1): 567-570.
Oktavia F, Kuswanhadi, Widodo, Dinarty D, Sudarsono. 2017. Pathogenicity and rDNA-ITS sequence analysis of the Corynespora cassiicola isolates from rubber plantations in Indonesia. Emirates J Food Agricul 29(11):872-883. doi: 10.9755/ejfa.2017.v29.i11.1497.
Othman R 2013. Development of high yielding disease resistance clones. IRRDB Agronomy Workshop Medan, Indonesia.
Petrov V, Qureshi MK, Hille J, Gechev T. 2018. Occurrence, biochemistry and biological effects of host-selective plant mycotoxins. Food Chem Toxic 112: 251-264. doi.org/10.1016/j.fct.2017.12.047
Pujade-Renaud V, Lopez D, Ribeiro S, Minh T, Deon M, Clement-Demange A, Garcia D, Drevet P, Label P, Morin E. 2015. The effectors of Corynespora cassiicola virulence in rubber tree. International Rubber Confrence 2015, Ho Chi Minh, Vietnam, Agriculture Publishing House.
Rampersad SN, Perez-Brito D, Torres-Calzada C, Tapia-Tussell R, Carrington CV. 2013. Genetic structure and demographic history of Colletotrichum gloeosporioides sensu lato and C. truncatum isolates from Trinidad and Mexico. BMC Evol Biol 13(1): 1-17. doi:10.1186/1471-2148-13-130
Ribeiro S, Tran DM, Deon M, Clément-Demange A, Garcia D, Soumahoro M, Masson A, Pujade-Renaud V. 2019. Gene deletion of Corynespora cassiicola cassiicolin Cas1 suppresses virulence in the rubber tree. Fungal Genet Biol 129: 101-114. doi.org/10.1016/j.fgb.2019.05.004
Shuib SH, Deon M, Mahyuddin MM, Izhar A, Fumanal B, Sunderasan E, Pujade-Renaud V. 2015. Cassiicolin genes among Corynespora cassiicola isolates from rubber plantations in Malaysia. J Rubber Res 18(2): 6241-6246.
Tran DM, Cle´ment-Demange A, De´on M, Garcia D, Le Guen V, Cle´ment-Vidal A, et al. 2016. Genetic Determinism of Sensitivity to Corynespora cassiicola Exudates in Rubber Tree (Hevea brasiliensis). PLoS ONE 11(10): e0162807. doi:10.1371/journal.pone.0162807
Wu J, Xie X, Shi Y, Chai A, Wang Q, li B. 2018. Variation of cassiicolin genes among Chinese isolates of Corynespora cassiicola. J Microbiol 56: 634–647. doi.org/10.1007/s12275-018-7497-5
Xianbao L, Boxun L, Shan C, Guixiu H. 2016. Diversity and Pathogenicity of the Cassiicolin Gene in Corynespora cassiicola of Rubber Tree in China. J Tropical Crops 10: 1969-1973
Xianbao L, Tao S, Yanli F. 2019. Analysis of host specificity andpathogenicity of Cas5 isoform cassiicolin in Corynespora cassiicola from Hevea brasiliensis.[abstract].Proceedings of the 2019 Annual Meeting of the Chinese Academy of Plant Pathology.
Barthe P, Pujade-Renaud V, Breton F, Gargani D, Thai R, Roumestand C, et al. 2007. Structural analysis of cassiicolin, a host-selective protein toxin from Corynespora cassiicola. J Mol Biol 367(1):89–101. doi: 10.1016/j.jmb.2006.11.086 PMID: 17234212
Boxun L, Xianbao L, Yanli F, Huiting X, Guixiu H. 2019. Construction of Cassiicolin Genes Barcode Database and Molecular Detection Technology of Corynespora cassiicola from Hevea brasiliensis in China. Chinese J Tropical Crops 40(9): 1770-1782.
Breton F, Sanier C, d’Auzac A. 2000. Role of cassiicolin a host-selective toxin in pathogenicity of Corynespora cassiicola, causal agent of a leaf fall disease of Hevea. J Rubb Res 3(2): 115-128.
de Lamotte F, Duviau MP, Sanier C, Thai R, Poncet J, Bieysse D, Breton F, Pujade-Renaud V. 2007. Purification and characterization of Cassiicollin toxin produced by Corynespora cassiicola causal agent of the leaf fall disease of rubber tree. J Chrom 849: 357-362.
Déon M, Bourré Y, Gimenez S, Berger A, Bieysse D, de Lamotte F, Poncet J, Roussel V, Bonnot F, Oliver G, et al. 2012a. Characterization of a cassiicolin-encoding gene from Corynespora cassiicola, pathogen of rubber tree (Hevea brasiliensis). Plant Sci 185–186: 227-237. doi:10.1016/j.plantsci.2011.10.017
Déon M, Scomparin A, Tixier A, Mattos CR, Leroy T, Seguin M, Roeckel-Drevet P, Pujade-Renaud V. 2012b. First characterization of endophytic Corynespora cassiicola isolates with variant cassiicolin genes recovered from rubber trees in Brazil. Fungal Diver 54(1): 87-99. doi:10.1007/s13225-012-0169-6
Déon M, Fumanal B, Gimenez S, Bieysse D, Oliveira RR, Shuib SS, Breton F, Elumalai S, Vida JB, Seguin M, et al. 2014. Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis. Fungal Biol 118(1): 32-47. doi:10.1016/j.funbio.2013.10.011
Dixon LJ, Schlub RL, Pernezny K, Datnoff LE. 2009. Host specialization and phylogenetic diversity of Corynespora cassiicola. Phytopathology 99(9): 1015-1027. doi:10.1094/PHYTO-99-9-1015
Drummond AJ, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, et al. (2012). Geneious v5.6.
Farr D, Rossman A. 2019. Fungal Databases, Systematic Mycology and Microbiology Laboratory, U.S. National Fungus Collections, ARS, USDA.
https://nt.ars-grin.gov/fungaldatabases/.
Fernando THPS, Jayasinghe CK, Wijesundera RLC, Silva WPK, Nishantha EADN. 2010. Evaluation of screening methods against Corynespora leaf fall disease of rubber (Hevea brasiliensis). J Plant Diseases Protec 117(1): 24-29. doi:10.2307/43229087
Timothy L. Friesen TL, Faris JD, Solomon PS, Oliver, RP. 2008. Host-specifific toxins: effectors of necrotrophic pathogenicity. Celular Microbiol 10(7): 1421–1428. doi:10.1111/j.1462-5822.2008.01153.x
Liu XB, Li BX, Chen S, Huang GX. 2016. Diversity and pathogenicity of the cassiicolin gene in Corynespora cassiicola of rubber tree in China. Chinese J Tropic Crops 37: 1969-1973.
Lopez D, Ribeiro S, Label P, Fumanal B, Venisse J-S, Kohler A, de Oliveira RR, Labutti K, Lipzen A, Lail K, Bauer D, Ohm RA, Barry KW, Spatafora J, Grigoriev IV, Martin FM and Pujade-Renaud V. 2018. Genome-Wide Analysis of Corynespora cassiicola Leaf Fall Disease Putative Effectors. Front Microbiol 9: 276. doi: 10.3389/fmicb.2018.00276
Nghia N, Kadir J, Sunderasan E, Abdullah M, Malik A, Napis S. 2008. Morphological and inter Simple Sequence Repeat (ISSR) Markers Analyses of Corynespora cassiicola Isolates from Rubber Plantations in Malaysia. Mycopathologia. 166: 189-201.
Ogbebor ON. 2010. The status of three common leaf disease of Para rubber in Nigeria. JAnimal Plant Sci 6(1): 567-570.
Oktavia F, Kuswanhadi, Widodo, Dinarty D, Sudarsono. 2017. Pathogenicity and rDNA-ITS sequence analysis of the Corynespora cassiicola isolates from rubber plantations in Indonesia. Emirates J Food Agricul 29(11):872-883. doi: 10.9755/ejfa.2017.v29.i11.1497.
Othman R 2013. Development of high yielding disease resistance clones. IRRDB Agronomy Workshop Medan, Indonesia.
Petrov V, Qureshi MK, Hille J, Gechev T. 2018. Occurrence, biochemistry and biological effects of host-selective plant mycotoxins. Food Chem Toxic 112: 251-264. doi.org/10.1016/j.fct.2017.12.047
Pujade-Renaud V, Lopez D, Ribeiro S, Minh T, Deon M, Clement-Demange A, Garcia D, Drevet P, Label P, Morin E. 2015. The effectors of Corynespora cassiicola virulence in rubber tree. International Rubber Confrence 2015, Ho Chi Minh, Vietnam, Agriculture Publishing House.
Rampersad SN, Perez-Brito D, Torres-Calzada C, Tapia-Tussell R, Carrington CV. 2013. Genetic structure and demographic history of Colletotrichum gloeosporioides sensu lato and C. truncatum isolates from Trinidad and Mexico. BMC Evol Biol 13(1): 1-17. doi:10.1186/1471-2148-13-130
Ribeiro S, Tran DM, Deon M, Clément-Demange A, Garcia D, Soumahoro M, Masson A, Pujade-Renaud V. 2019. Gene deletion of Corynespora cassiicola cassiicolin Cas1 suppresses virulence in the rubber tree. Fungal Genet Biol 129: 101-114. doi.org/10.1016/j.fgb.2019.05.004
Shuib SH, Deon M, Mahyuddin MM, Izhar A, Fumanal B, Sunderasan E, Pujade-Renaud V. 2015. Cassiicolin genes among Corynespora cassiicola isolates from rubber plantations in Malaysia. J Rubber Res 18(2): 6241-6246.
Tran DM, Cle´ment-Demange A, De´on M, Garcia D, Le Guen V, Cle´ment-Vidal A, et al. 2016. Genetic Determinism of Sensitivity to Corynespora cassiicola Exudates in Rubber Tree (Hevea brasiliensis). PLoS ONE 11(10): e0162807. doi:10.1371/journal.pone.0162807
Wu J, Xie X, Shi Y, Chai A, Wang Q, li B. 2018. Variation of cassiicolin genes among Chinese isolates of Corynespora cassiicola. J Microbiol 56: 634–647. doi.org/10.1007/s12275-018-7497-5
Xianbao L, Boxun L, Shan C, Guixiu H. 2016. Diversity and Pathogenicity of the Cassiicolin Gene in Corynespora cassiicola of Rubber Tree in China. J Tropical Crops 10: 1969-1973
Xianbao L, Tao S, Yanli F. 2019. Analysis of host specificity andpathogenicity of Cas5 isoform cassiicolin in Corynespora cassiicola from Hevea brasiliensis.[abstract].Proceedings of the 2019 Annual Meeting of the Chinese Academy of Plant Pathology.
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