The combination of mare's milk and grape polyphenol extract for treatment of dysbiosis induced by dextran sulfate sodium




Abstract. Kozhakhmetov S, Babenko D, Nurgaziyev M, Tuyakova A, Nurgozhina A, Muhanbetganov N, Chulenbayeva L, Sergazy S, Gulyayev A, Saliev T, Kushugulova A. 2020. The combination of mare's milk and grape polyphenol extract for treatment of dysbiosis induced by dextran sulfate sodium. Biodiversitas 21: 2275-2280. This study showed the potential of a biological product based on mare's milk and a complex of grape polyphenols to modulate intestinal microflora after dextran sulfate sodium  (DSS)-induced dysbiosis. Rat ulcerative colitis has been developed using intra-gastric administration of 10% DSS solution. To track changes in the structure of the microbiome at all stages of the study, the next-generation sequencing of the 16S rRNA gene section and LotuS conveyor were used. The results of sequencing demonstrated a decrease in biological diversity of microbiota after the induction of colitis, and recovery after 7 days of use of the  (MMGPE). The product induced the structural changes of the microbiome damaged by DSS. Representatives of SCFA producing bacteria increased concentrations of  Prevotella, Alloprevotella, Lactobacillus, Ruminococcaceae, and Blautia.


Chen M, YiL, Zhang Y, Zhou X, Ran L, Yang J, Zhu J, Zhang Q, MiM. 2016. Resveratrol Attenuates Trimethylamine-N-Oxide (TMAO)-Induced Atherosclerosis by Regulating TMAO Synthesis and Bile Acid Metabolism via Remodeling of the Gut Microbiota. MBio 7: e02210-e2215.
Chong J, Liu P, Zhou G, Xia J. 2020. Using MicrobiomeAnalyst for comprehensive statistical, functional, and meta-analysis of microbiome data. Nat. Protoc. 150 :799–821.
Costello SP, Hughes PA, Waters O, Bryant RV, Vincent AD, Blatchford P, Katsikeros R, Makanyanga J, Campaniello MA, Mavrangelos C, Rosewarne CP, Bickley C, Peters C, Schoeman MN, Conlon MA, Roberts-Thomson IC, Andrews JM. 2019. Effect of Fecal Microbiota Transplantation on 8-Week Remission in Patients With Ulcerative Colitis: A Randomized Clinical Trial. JAMA 321: 156–164.
Danilova N, Abdulkhakov S, Grigoryeva T, Markelova M, Vasilyev I, Boulygina E, Ardatskaya M, Pavlenko A, Tyakht A, Odintsova A, Abdulkhakov R. 2019. Markers of dysbiosis in patients with ulcerative colitis and Crohn’s disease. Ter. Arkh. 91: 17-24.
Derwa Y, Gracie DJ, Hamlin PJ, Ford AC. 2017. Systematic review with meta-analysis: the efficacy of probiotics in inflammatory bowel disease. Aliment. Pharmacol. Ther. 46: 389-400.
Etxeberria U, Arias N, Boqué N, Macarulla MT, Portillo MP, Martínez JA, Milagro FI. 2015. Reshaping faecal gut microbiota composition by the intake of trans-resveratrol and quercetin in high-fat sucrose diet-fed rats. J. Nutr. Biochem. 26: 651-660.
Fotschki J, Szyc A.M, Laparra J.M, Markiewicz L.H, Wróblewska B, 2016. Immune-modulating properties of horse milk administered to mice sensitized to cow milk. J. Dairy Sci. 99: 9395-9404.
Gao Z, Chen K-Y, Mueller O, Zhang H, Rakhilin N., Chen J, Shen X. 2018. Microbiota of Inflammatory Bowel Disease Models. 0th International Engineering in Medicine and Biology Conference, Honolulu, Hawaii, July 17-21, 2018. 2374–2377.
Hall AB, Yassour M, Sauk J, Garner A, Jiang X, Arthur T, Lagoudas GK, Vatanen T, Fornelos N, Wilson R, Bertha M, Cohen M, Garber J, Khalili H, Gevers D, Ananthakrishnan AN, Kugathasan S, Lander ES, Blainey P, Vlamakis H, Xavier RJ, Huttenhower C. 2017. A novel Ruminococcus gnavus clade enriched in inflammatory bowel disease patients. Genome Med. 9: 103.
Hildebrand F, Tadeo R, Voigt AY, Bork P, Raes J. 2014. LotuS: an efficient and user-friendly OTU processing pipeline. Microbiome 2: 30-37.
Hu Y, Chen D, Zheng P, Yu J, He J, Mao X, Yu B. 2019. The Bidirectional Interactions between Resveratrol and Gut Microbiota: An Insight into Oxidative Stress and Inflammatory Bowel Disease Therapy. Biomed Res. Int. 5403761.
Järbrink-Sehgal E, Andreasson A. 2020. The gut microbiota and mental health in adults. Curr. Opin. Neurobiol. 62: 102-114.
McMurdie PJ, Holmes S. 2013. phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data. PLoS One 8: e61217.
Moayyedi P, Surette MG, Kim PT, Libertucci J, Wolfe,M, Onischi C, Armstrong D, Marshall JK, Kassam Z, Reinisch W, Lee CH. 2015. Fecal Microbiota Transplantation Induces Remission in Patients With Active Ulcerative Colitis in a Randomized Controlled Trial. Gastroenterology 149: 102-109.
Naito Y, Uchiyama K, Takagi T. 2018. A next-generation beneficial microbe: Akkermansia muciniphila. J. Clin. Biochem. Nutr. 63: 33-35.
Oksanen J, Blanchet FG, Friendly M, Kindt R, Legendre P, Mcglinn D, et al. Package “vegan” Title Community Ecology Package. Community Ecol Packag. 2019. 2(9): 1-297. Available from:
Pei L, Ke Y, Zhao H, Wang L, Jia C, Liu W, Fu Q, Shi M, Cui J, Li S. 2019. Role of colonic microbiota in the pathogenesis of ulcerative colitis. BMC Gastroenterol. 19: 10.
Png CW, Lindén SK, Gilshenan KS, Zoetendal EG, McSweeney CS, Sly LI, McGuckin MA, Florin THJ. 2010. Mucolytic bacteria with increased prevalence in IBD mucosa augment in vitro utilization of mucin by other bacteria. Am. J. Gastroenterol. 105: 2420-2428.
Rather IA, Bajpai VK, Ching LL, Majumder R, Nam G-J, Indugu N, Singh P, Kumar S, Hajrah NH, Sabir JSM, Kamli MR, Park Y-H. 2020. Effect of a bioactive product SEL001 from Lactobacillus sakei probio65 on gut microbiota and its anti-colitis effects in a TNBS-induced colitis mouse model. Saudi J. Biol. Sci. 27: 261-270.
Shen Z-H, Zhu C-X, Quan Y-S, Yang Z-Y, Wu S, Luo W-W, Tan B, Wang X-Y. 2018. Relationship between intestinal microbiota and ulcerative colitis: Mechanisms and clinical application of probiotics and fecal microbiota transplantation. World J. Gastroenterol. 24: 5-14.
Slavin J. 2013. Fiber and prebiotics: mechanisms and health benefits. Nutrients 5: 1417-1435.
Snopek L, Mlcek J, Sochorova L, Baron M, Hlavacova I, Jurikova T, Kizek R, Sedlackova E, Sochor J. 2018. Contribution of Red Wine Consumption to Human Health Protection. Molecules 23: 1684.
Tian Y, Zhou Y, Huang S, Li J, Zhao K, Li X, Wen X., Li X. 2019. Fecal microbiota transplantation for ulcerative colitis: a prospective clinical study. BMC Gastroenterol. 19: 116.
Wan MLY, Co VA, El-Nezami H. 2020. Dietary polyphenol impact on gut health and microbiota. Crit. Rev. Food Sci. Nutr. 1-22.
Wang J, Xiong K, Zhao S, Zhang C, Zhang J, Xu L, Ma A. 2020. Long-Term Effects of Multi-Drug-Resistant Tuberculosis Treatment on Gut Microbiota and Its Health Consequences. Front. Microbiol. 11: 53.
Xie J, Liu Y, Chen B, Zhang G, Ou S, Luo J, Peng X. 2019. Ganoderma lucidum polysaccharide improves rat DSS-induced colitis by altering cecal microbiota and gene expression of colonic epithelial cells. Food Nutr. Res. 63: 1559.

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