Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit
##plugins.themes.bootstrap3.article.sidebar##
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Hidayat H, Haryadi W, Matsjeh S, Raharjo TJ. 2019. Molecular identification 16S rRNA gene of active proteolytic lactic acid bacteria (LAB) isolated from kelengkeng (Dimocarpus longan) fruit. Biodiversitas 20: 2222-2228. Various fermentation food involves microorganisms, especially Lactic Acid Bacteria (LAB) which has beneficial properties for fermented food. The present study aims to do identification and characterization of active proteolytic LAB from kelengkeng (Dimocarpus longan), a commonly found fruit in South East Asia. From ten LAB isolates, isolates K7 and K8 appeared as basil shape based on Gram staining and were chosen for further examination. The molecular identification of these selected isolates were investigated from the 16S rRNA analysis using universal primer 27F and 1525R. The K7 and K8 isolates showed resistance ability towards acidic environment (pH 2.0) and were examined for protease enzyme screening resulting 7.5 and 6 to clear zone, which was 17 and 12 mm. The result of the molecular characterization from the two isolates indicated that isolate K7 was identified as Leuconostoc mesenteroides subsp suionicum strain LT-38 while isolate K8 was identified as Leuconostoc mesenteroides strain C305.16.
##plugins.themes.bootstrap3.article.details##
Emerenini EC, Afolabi OR, Okolie PI, Akintokun AK. 2013. Isolation and Molecular Characterization of Lactic Acid Bacteria Isolated from Fresh Fruits and Vegetables Using Nested PCR Analysis. British Microbiology Research Journal 3: 368-377.
Evi T, Eko Y, Novik N. 2006. The viability test of Lactobacillus sp. Mar 8 is encapsulated, Biodiversity 7: 114-117.
Feriala AA, Abu S. 2016. Efficacy of Lactic Acid Bacteria Isolated from Some Fruits and Vegetables, Egypt Journal Microbiol 51: 13- 28.
Garcia EF, Luciano WA, Xavier DE, da Costa EC, de Sousa OK, Franco OL, de Morais JMA, Lucena BT, Picão RC, Magnani M, Saarela M, de Souza EL. 2016. Identification of Lactic Acid Bacteria in Fruit Pulp Processing By products and Potential Probiotic Properties of Selected Lactobacillus Strains, Journal Front Microbial 30: 1371.
Habibi H. 2017. Analysis of 16S rRNA gene Lactic Acid Bacteria (LAB) Isolate From Markisa Fruit (Passiflora sp.) as a producer of enzyme and probiotics; AIP Conference Proceedings International Conference on Chemistry , Chemical Process and Engineering in Java Island. Universitas Islam Indonesia, Yogyakarta, 15-16 November2016. [Indonesia]
Joddi IC, Winarti Z, Desniar. 2007. Isolation and Characterization of Lactic Acid Bacteria From Bandeng (Chanos chanos) Bekasam, Aquatic Product Technology 2 : 10.
Joko S.2017. Kelengkeng Superior Variety. Jakarta Baru Press, Yogyakarta.
Kwok J. De Vos W.M. 1994. The Proteolytic System of Lactic Acid Bacteria. In : Genetics and Biotechnology of Lactic Acid Bacteria. Ed Gasson abd De Vos. Balckie Academic & Profesional, Glasgow.
Labeda DP. 1990. Isolation Biotechnological Organisme From Nature Environmental Biotechnology. Mc. Graw Hill Publishing Company, USA.
Loveness KN, Martinus JR, Nout, Tendekayi HG, Bar T, Teun B, Marcel HZ. 2007. Yeasts and Lactic Acid Bacteria Microbiota from Masau (Ziziphus mauritiana) Fruits and Their Fermented Fruit Pulp in Zimbabwe, International Journal of Food Microbiology 120: 159–166.
Nurmalinda A, Periadnadi, Nurmiati. 2013. Isolation and Partial Characterization of Indigenous Fermenting Bacteria From Durian Fruit (Durio zibethinus Murr.), Journal of Biology 2 : 12.
Rani Y, Titania T, Nugroho, Fifi P. 2015. Test of Enzyme Protease Activity of Isolate Bacillus sp. Riau Local Channels. JOM FMIPA 1 : 1-12.
Rosalia T, Lluís B, Emilio M, Esther B. 2008. Lactic Acid Bacteria from Fresh Fruit and Vegetables as Biocontrol Agents of Phytopathogenic Bacteria and Fungi. Journal International Microbiology 1: 231-236.
Rosiana P. 2009. Isolation of Bacteria Test of Thermophilic Protease Activity from Hot Spring the Sipoholon North Tapanuli. Universitas Sumatera Utara, Medan.
Soeharsono. 2010. Probiotics Scientific Base, Application and Practical Aspects. Widya Padjajaran, Bandung.
Surono IS. 2004. Probiotics; Fermentation Milk and Health. Cipta Karya, Jakarta.
Widodo L. 2007. Microbiology. Universitas Terbuka, Jakarta.
Wikandari P, Suparmo, Marsono Y, Rahayu E. 2012. The Characterization of Proteolytic Lactic Acid Bacteria in the Bekasam, Journal Nature Indonesia 14: 120-125.
Wilda LS, Sumaryati S, Jamsari. 2013. Optimization of Protease Activity from Lactic Acid bacteria (LAB) Pediococcus pentosaceus Isolated from Soursop fermentation (Annona muricata L.), Andalas University. Journal of Chemistry 2: 1.
Yusmarini Y, Usman P, Vonny SJ, Akhyar A, Kusumaningrum. 2016. Characterization of Amylolytic Lactic Acid Bacteria from Sago Starch Processing Industry, AGRITECH 37 : 95-100.
Most read articles by the same author(s)
- HABIBI HIDAYAT, WINARTO HARYADI, TRI JOKO RAHARJO, Three-dimensional structure modeling of a protease from lactic acid bacteria Leuconostoc mesenteroides K7 using automated protein homology analysis , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 7 (2020)