Pharmacognostic authentication and bioactivity of polyherbal essential oils from red ginger, turmeric, and aromatic ginger
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Abstract. Hamad A, Wahyuningrum R, Pusparini DH, Faridaeni D, Isabila EM, Hartanti D. 2025. Pharmacognostic authentication and bioactivity of polyherbal essential oils from red ginger, turmeric, and aromatic ginger. Biodiversitas 26: 5132-5144. People in Baturraden, Indonesia, use rhizomes of red ginger (Zingiber officinale var. rubrum), turmeric (Curcuma longa), and aromatic ginger (Kaempferia galanga) to make polyherbal formulations for managing masuk angin symptoms. This study aimed to verify the identity of the crude drug, determine the antioxidant properties of the formulations, and evaluate the chemical constituents and antimicrobial activity of polyherbal essential oils to assess the effectiveness for curing masuk angin symptoms. The identity of the red ginger, turmeric, and aromatic ginger crude drugs was conducted by following the pharmacopoeial method. The crude drugs were prepared into seven formulations, designated as Formula 1 through Formula 7. The antioxidant properties were evaluated using the standard 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity method, and the Total Phenolic Content (TPC) was determined using UV-Vis spectrophotometry. The antimicrobial activity was determined using the microdilution method, with the percentage of inhibition as a parameter. The polyherbal formulations exerted different DPPH scavenging activity and TPC, with the highest values observed in Formula 1[red ginger only, 178.01±3.88 mmol Trolox Equivalent (TE)/g] and Formula 2 [turmeric only, 9.60±0.28 mg Gallic Acid Equivalent (GAE)/g], respectively. Each polyherbal essential oil contained a different constituent profile. Polyherbal essential oils showed moderate antimicrobial activity against Multidrug-Resistant (MDR) Streptococcus aureus and Candida albicans, with more profound antifungal activity than antimicrobial activity. Formula 3 demonstrated the highest overall antimicrobial activity, with percentages of inhibition against Streptococcus aureus and Candida albicans were 15.75±1.32% and 71.67±3.39%, respectively. The findings confirmed the identity of the crude drugs, highlighting the potential of a local polyherbal formulation, particularly Formula 3, for its antioxidant and moderate antimicrobial activities associated with masuk angin symptoms.
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References
Afendi FM, Heryanto R, Darusman LK, Syahrir NHA, Bakri R, Qomariasih N. 2016. Jamu informatics: A new perspective in jamu research. CICSJ Bull 34 (2): 47-52. DOI: 10.11546/CICSJ.34.47.
Ahmad RS, Hussain MB, Sultan MT, Arshad MS, Waheed M, Shariati MA, Plygun S, Hashempu MH. 2020. Biochemistry, safety, pharmacological activities, and clinical applications of turmeric: A mechanistic review. Evid Based Complement Alternat Med 2020: 7656919. DOI: 10.1155/2020/7656919.
Albaqami JJ, Hamdi H, Narayanankutty A, Visakh NU, Sasidharan A, Kuttithodi AM, Famurewa AC, Pathrose B. 2022. Chemical composition and biological activities of the leaf essential oils of Curcuma longa, Curcuma aromatica and Curcuma angustifolia. Antibiotics 11 (11): 1547. DOI: 10.3390/antibiotics11111547.
Amarante JF, da Costa MM, da Silva Almeida JRG, de Oliveira HP, 2023. Synergistic interaction of clove, cinnamon, and eucalyptus essential oils impregnated in cellulose acetate electrospun fibers as antibacterial agents against Staphylococcus aureus. Braz J Microbiol 54 (3): 1635-1643. DOI: 10.1007/s42770-023-01048-5.
Angane M, Swift S, Huang K, Butts CA, Quek SY. 2022. Essential oils and their major components: An updated review on antimicrobial activities, mechanism of action and their potential application in the food industry. Foods 11 (3): 464. DOI: 10.3390/foods11030464.
Asoka SF, Wahyuni WT, Batubara I, Wahyudi ST. 2022. Red ginger (Zingiber officinale var. rubrum): Its essential oil content and potential as an anti-Propionibacterium acnes by molecular docking. AIP Conf Proc 2563: 050003. DOI: 10.1063/5.0103211.
Badrunanto, Wahyuni WT, Farid M, Batubara I, Yamauchi K. 2024. Antioxidant components of the three different varieties of Indonesian ginger essential oil: In vitro and computational studies. Food Chem Adv 4: 100558. DOI: 10.1016/j.focha.2023.100558.
Barbosa GB, Minguillan JMO. 2021. Antioxidant activity and total phenolic content of fresh and cured rhizomes of Curcuma longa and Etlingera philippinensis. Intl Food Res J 28 (4): 839-847. DOI: 10.47836/ifrj.28.4.20.
Batubara I, Badrunanto, Wahyuni WT, Farid M. 2023. Combination of extraction and distillation of red ginger rhizome on the composition of active compounds and tyrosinase inhibitory activity. Intl J Adv Sci Eng Inf Technol 13 (2): 431-437. DOI: 10.21462/ijaseit.v13i2.17606.
Bhattacharya R, Rolta R, Dev K, Sourirajan A. 2021. Synergistic potential of essential oils with antibiotics to combat fungal pathogens: Present status and future perspectives. Phytother Res 35 (11): 6089-6100. DOI: 10.1002/ptr.7218.
de Araújo GA, de Morais Oliveira Tintino CD, Pereira RLS et al. 2025. Toxicological assessment of citral and geraniol: Efflux pump inhibition in Staphylococcus aureus and invertebrate toxicity. Toxicol Rep 14: 101917. DOI: 10.1016/j.toxrep.2025.101917.
de Lima PMN, Pereira TC, de Carvalho LS, dos Santos LF, Oliveira CER, de Paula Ramos L, Marcucci MC, Hasna AA, de Oliveira LD. 2024. Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans, Staphylococcus aureus, and Candida spp. World J Crit Care Med 13 (3): 92531. DOI: 10.5492/wjccm.v13.i3.92531.
de Sousa DP, Damasceno ROS, Amorati R, Elshabrawy HA, de Castro RD, Bezerra DP, Nunes VRV, Gomes RC, Lima TC. 2023. Essential oils: Chemistry and pharmacological activities. Biomolecules 13 (7): 1144. DOI: 10.3390/biom13071144.
El Hachlafi N, Aanniz T, El Menyiy N, El Baaboua A, El Omari N, Balahbib A, Shariati MA, Zengin G, Fikri-Benbrahim K, Bouyahya A. 2023. In vitro and in vivo biological investigations of camphene and its mechanism insights: A review. Food Rev Intl 39 (4): 1799-1826. DOI: 10.1080/87559129.2021.1936007.
Ghasemzadeh A, Jaafar HZE, Rahmat A. 2016. Variation of the phytochemical constituents and antioxidant activities of Zingiber officinale var. rubrum Theilade associated with different drying methods and polyphenol oxidase activity. Molecules 21 (6): 780. DOI: 10.3390/molecules21060780.
Guerrini A, Tacchini M, Chiocchio I, Grandini A, Radice M, Maresca I, Paganetto G, Sacchetti G. 2023. A comparative study on chemical compositions and biological activities of four Amazonian Ecuador essential oils: Curcuma longa L. (Zingiberaceae), Cymbopogon citratus (DC.) Stapf, (Poaceae), Ocimum campechianum Mill. (Lamiaceae), and Zingiber officinale R. Antibiotics 12 (1): 177. DOI: 10.3390/antibiotics12010177.
Guimarães AF, Vinhas ACA, Gomes AF, Souza LH, Krepsky PB. 2020. Essential oil of Curcuma longa L. rhizomes chemical composition, yield variation and stability. Quim Nova 43 (7): 909-913. DOI: 10.21577/0100-4042.20170547.
Hamad A, Djalil AD, Dewi DYS, Hartanti D. 2021. Development of lemon basil essential oil as a natural chicken meat preservative. IOP Conf Ser: Earth Environ Sci 803: 012028. DOI: 10.1088/1755-1315/803/1/012028.
Haroen U, Syafwan S, Kurniawan K, Budiansyah A. 2024. Determination of total phenolics, flavonoids, and testing of antioxidant and antibacterial activities of red ginger (Zingiber officinale var. rubrum). J Adv Vet Anim Res 11 (1): 114-124. DOI: 10.5455/javar.2024.k755.
Hartanti D, Chatsumpun N, Sa-ngiamsuntorn K, Supharattanasitthi W, Kitphati W, Peungvicha P. 2023. The pharmacognostic standards, antioxidant and antidiabetic activities, and hepatic safety profile of an Indonesian antidiabetic polyherbal formulation. Indones J Pharm 34 (1): 65-78. DOI: 10.22146/ijp.3243.
Hartanti D, Hamad A. 2023. Antioxidant properties and interaction effects of a novel polyherbal formulation. Curr Trends Biotechnol Pharm 17 (4A): 28-33. DOI: 10.5530/ctbp.2023.4s.87.
Hartanti D, Insani AA, Wahono SK, Hamad A. 2024. Extraction method and crude drug-to-solvent ratio effects on the antioxidant properties and physicochemical profile during storage of a polyherbal formulation extract. BIO Web Conf 135: 06002. DOI: 10.1051/bioconf/202413506002.
Hartanti D, Romdhoni MF, Hamad A. 2025. Crude drug standardization and antioxidant properties of an Indonesian antidiabetic polyherbal formulation and plant constituents. Trop J Nat Prod Res 9 (5): 2074-2080. DOI: 10.26538/tjnpr/v9i5.28.
Hearunyakij M, Phutdhawong W. 2022. Optimization, yield and chemical composition of essential oil from Kaempferia galanga L. rhizome: comparative study with microwave assisted extraction and hydrodistillation. J Essent Oil Bear Plants 25 (3): 444-455. DOI: 10.1080/0972060X.2022.2108727.
Heinrich M, Barnes J, Prieto-Garcia J, Gibbon S, Williamson EM. 2023. The complex pharmacology of herbal medicines. In: Heinrich M, Barnes J, Prieto-Garcia J, Gibbon S, Williamson EM (eds). Fundamentals of Pharmacognosy and Phytotherapy. Elsevier, Amsterdam, Netherlands.
Hulankova R. 2024. Methods for determination of antimicrobial activity of essential oils in vitro - A review. Plants 13 (19): 2784. DOI: 10.3390/plants13192784.
Indonesian MoH. 2017. Indonesian Herbal Pharmacopeia. Ministry of Health, Republic of Indonesia, Jakarta. [Indonesian]
Ivanov M, Kannan A, Stojkovi? DS, Glamo?lija J, Calhelha RC, Ferreira ICFR, Sanglard D, Sokovi? M. 2021. Camphor and eucalyptol - anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. Intl J Mol Sci 22: 483. DOI: 10.3390/ijms22020483.
Ju J, Xie Y, Yu H, Guo Y, Cheng Y, Qian H, Yao W. 2022. Synergistic interactions of plant essential oils with antimicrobial agents: A new antimicrobial therapy. Crit Rev Food Sci Nutr 62 (7): 1740-1751. DOI: 10.1080/10408398.2020.1846494.
Kumar A. 2020. Phytochemistry, pharmacological activities and uses of traditional medicinal plant Kaempferia galanga L. - an overview. J Ethnopharmacol 253: 112667. DOI: 10.1016/j.jep.2020.112667.
Lukiati B, Sulisetijono, Nugrahaningsih, Masita R. 2020. Determination of total phenol and flavonoid levels and antioxidant activity of methanolic and ethanolic extract of Zingiber officinale Rosc var. rubrum rhizome. AIP Conf Proc 2231: 040003. DOI: 10.1063/5.0002657.
Lukita S, Khosasi W, Susanto C, Florenly. 2021. The antibacterial effectiveness of red ginger (Zingiber officinale Roscoe) essential oil in inhibiting the growth of Staphylococcus aureus and Streptococcus mutans. Biomed J Indones 7: 364-373. DOI: 10.32539/BJI.v7i2.305.
Lukitaningsih E, Rohman A, Rafi M, Nurrulhidayah AF, Windarsih A. 2020. In vivo antioxidant activities of Curcuma longa and Curcuma xanthorrhiza: A review. Food Res 4 (1): 13-19. DOI: 10.26656/fr.2017.4(1).172.
Mapeka TM, Sandasi M, Viljoen AM, van Vuuren SF. 2022. Optimization of antioxidant synergy in a polyherbal combination by experimental design. Molecules 27 (13): 4196. DOI: 10.3390/molecules27134196.
Muderawan IW, Mudianta IW, Martiningsih NW. 2022. Physicochemical properties, chemical compositions and antioxidant activities of rhizome oils from two varieties of Kaempferia galanga. Indones J Chem 22 (1): 72-85. DOI: 10.22146/ijc.66348.
Muflihah YM, Gollavelli G, Lin Y-C. 2021. Correlation study of antioxidant activity with phenolic and flavonoid compounds in 12 Indonesian indigenous herbs. Antioxidants 10 (10): 1530. DOI: 10.3390/antiox10101530.
Mugao L. 2024. Factors influencing yield, chemical composition and efficacy of essential oils. Intl J Multidiscip Res Growth Eval 5 (4): 169-178. DOI: 10.54660/.ijmrge.2024.5.4.169-178.
Murudkar PH, Tambe MS, Chandrasekar SB, Boddeda B, Pawar AT. 2022. Common Ayurvedic, Chinese traditional and Unani antidiabetic formulations - A review. Front Pharmacol 13: 991083. DOI: 10.3389/fphar.2022.991083.
Muzzazinah, Yunus A, Rinanto Y, Suherlan Y, Ramli M, Putri DS, Ningtyas DW, Rahma AL, Nabila SJ. 2024. Profile of chemical compounds and potency of galangal (Kaempferia galanga L.) essential oils from Kemuning Village, Karanganyar District, Central Java, Indonesia. Biodiversitas 25 (4): 1386-1393. DOI: 10.13057/biodiv/d250406.
Ngo-Mback MNL, Babii C, Dongmo PMJ, Toghueo MRK, Stefan M, Boyom FF. 2020. Anticandidal and synergistic effect of essential oil fractions from three aromatic plants used in Cameroon. J Mycol Med 30 (2): 100940. DOI: 10.1016/j.mycmed.2020.100940.
Nissa A, Utami R, Sari AM, Nursiwi A. 2018. Combination effect of nisin and red ginger essential oil (Zingiber officinale var. rubrum) against foodborne pathogens and food spoilage microorganisms. AIP Conf Proc 2014: 020023. DOI: 10.1063/1.5054427.
Nurjanah S, Rosalinda S, Andina DP. 2024. Optimization of red ginger (Zingiber officinale var. rubrum) extraction using microwave assisted hydrodistillation method. Jurnal Teknik Pertanian Lampung 13 (2): 369-380. DOI: 10.23960/jtep-l.v13i2.369-380.
Orellana-Paucar AM. 2024. Turmeric essential oil constituents as potential drug candidates: A comprehensive overview of their individual bioactivities. Molecules 29: 4210. DOI: 10.3390/molecules29174210.
Paul S, Majumdar M. 2022. Comparative study of six antidiabetic polyherbal formulation for its multimodal approaches in diabetes management. 3 Biotech 12: 114. DOI: 10.1007/s13205-022-03166-7.
Paul S, Majumdar M. 2023. Multimode assessment of commercial polyherbal formulation: An in vitro and in silico approach. Appl Biochem Biotechnol 195 (4): 2261-2281. DOI: 10.1007/s12010-022-04064-7.
Prayoga T, Pradipto YD. 2014. Masuk angin in Javanese healthy lifestyle: A qualitative analysis using health and indigenous psychology approaches. ANIMA Indones Psychol J 29 (3): 146-154. DOI: 10.24123/aipj.v29i3.
Qiang Y, Si R, Tan S, Wei H, Huang B, Wu M, Shi M, Fang L, Fu J, Zeng S. 2021. Spatial variation of volatile organic compounds and antioxidant activity of turmeric (Curcuma longa L.) essential oils harvested from four provinces of China. Curr Res Food Sci 4: 882-890. DOI: 10.1016/j.crfs.2021.11.002.
Rahim NFA, Muhammad N, Abdullah N, Talip BA, Poh KH. 2020. The interaction effect and optimal formulation of selected polyherbal extracts towards antioxidant activity. Food Res 4 (6): 2042-2048. DOI: 10.26656/fr.2017.4(6).281.
Rinanda T, Isnanda RP, Zulfitri. 2018. Chemical analysis of red ginger (Zingiber officinale Roscoe var rubrum) essential oil and its anti-biofilm activity against Candida albicans. Nat Prod Commun 13 (12): 1587-1590. DOI: 10.1177/1934578X1801301206.
Sagar PK, Sharma P, Singh R. 2024. Anti-quorum sensing and anti-biofilm activity of ginger (Zingiber officinale) rhizomes against multidrug-resistant clinical isolates of Pseudomonas aeruginosa. Avicenna J Med Biotechnol 16 (1): 49-56. DOI: 10.18502/ajmb.v16i1.14171.
Sandrasari DA, Sabariman M, Azni IN. 2019. Determination of potential level of Indonesian rhizomes as an antioxidant based on phenolic compound and antioxidant activity. IOP Conf Ser: Earth Environ Sci 383: 012017. DOI: 10.1088/1755-1315/383/1/012017.
Sharma N, Gupta N, Orfali R, Kumar V, Patel CN, Peng J, Perveen S. 2022. Evaluation of the antifungal, antioxidant, and anti-diabetic potential of the essential oil of Curcuma longa leaves from the North-Western Himalayas by in vitro and in silico analysis. Molecules 27 (22): 7664. DOI: 10.3390/molecules27227664.
Sharma V, Gogoi B, Borah SN, Ghosh A, Mazumdar A, Kalita RD. 2024. In-silico molecular docking and molecular dynamic simulation of ?-elemene and caryophyllene identified from the essential oil of Kaempferia galanga L. against biofilm-forming proteins, CrtM and SarA of Staphylococcus aureus. J Biomol Struct Dyn 7: 1-13. DOI: 10.1080/07391102.2024.2310773.
Sivasothy Y, Chong WK, Hamid A, Eldeen IM, Sulaiman SF, Awang K. 2011. Essential oils of Zingiber officinale var. rubrum Theilade and their antibacterial activities. Food Chem 124 (2): 514-517. DOI: 10.1016/j.foodchem.2010.06.062.
Sobhy M, Ali SS, Cui H, Lin L, El-Sapagh S. 2023. Exploring the potential of 1,8-cineole from cardamom oil against food-borne pathogens: Antibacterial mechanisms and its application in meat preservation. Microb Pathog 184: 106375. DOI: 10.1016/j.micpath.2023.106375.
Tang Z, Chen H, Zhang M, Fan Z, Zhong Q, Chen W, Yun Y-H, Chen W. 2022. Antibacterial mechanism of 3-carene against the meat spoilage bacterium Pseudomonas lundensis and its application in pork during refrigerated storage. Foods 11 (1): 92. DOI: 10.3390/foods11010092.
Triratnawati A. 2011. Masuk angin dalam konteks kosmologi Jawa. Humaniora 23: 326-335. DOI: 10.22146/jh.1033. [Indonesian]
Utaminingrum W, Nofrianti, Hartanti D. 2021. Ethnopharmacological study of the polyherbal formula in Baturraden, Indonesia. Suranaree J Sci Technol 28 (5): 070024(1-7).
Wahyuni DR, Widowati W, Kusuma HSW, Pratami HK, Wijayanti CR, Wahyuni CD. 2021. Antioxidant potency of Kaempferia galanga Linn and Zingiber officinale var. rubra rhizomes. 2021 IEEE International Conference on Health, Instrumentation & Measurement, and Natural Sciences (InHeNce), 2021, Medan, Indonesia. DOI: 10.1109/InHeNce52833.2021.9537220.
Wang S-Y, Cai L, Yang N, Xu F-F, Wu Y-S, Liu B. 2023. Chemical composition of the Kaempferia galanga L. essential oil and its in vitro and in vivo antioxidant activities. Front Nutr 10: 1080487. DOI: 10.3389/fnut.2023.1080487.
Wang S-Y, Zhao H, Xu H-T, Han X-D, Wu Y-S, Xu F-F, Yang X-B, Göransson U, Liu B. 2021. Kaempferia galanga L.: Progresses in phytochemistry, pharmacology, toxicology and ethnomedicinal uses. Front Pharmacol 12: 675350. DOI: 10.3389/fphar.2021.675350.
Widyowati R, Agil M. 2018. Chemical constituents and bioactivities of several Indonesian plants typically used in jamu. Chem Pharm Bull 66 (5): 506-518. DOI: 10.1248/cpb.c17-00983.
Wijayanti II, Budiharjo A, Pangastuti A, Prihapsara F, Artanti AN. 2018. Total phenolic content and antioxidant activity of ginger extract and SNEDDS with eel fish bone oil (Anguilla spp.). Nusantara Bioscience 10: 164-169. DOI: 10.13057/nusbiosci/n100306.
Winarni E, Nasution AN, Nasution SW. 2023. Antifungal activity of red ginger (Zingiber officinale var. rubrum) and garlic (Allium sativum) against HIV patients-isolated Candida albicans. Jurnal Penelitian Pendidikan IPA 9 (10): 9038-9044. DOI: 10.29303/jppipa.v9i10.4981.
Xu F-X, Zhang J-Y, Jin J, Li Z-G, She Y-B, Lee M-R. 2021. Microwave-assisted natural deep eutectic solvents pretreatment followed by hydrodistillation coupled with GC-MS for analysis of essential oil from turmeric (Curcuma longa L.). J Oleo Sci 70 (10): 1481-1494. DOI: 10.5650/jos.ess20368.
Yang JJ, Rahmawati F. 2022. Antimicrobial effects of various red ginger (Zingiber officinale) extract concentrations on Escherichia coli bacteria. Eur J Biotechnol Biosci 10: 63-67.
Yang Q-Q, Cheng L-Z, Zhang T, Yaron S, Jiang H-X, Sui Z-Q, Corke H. 2020. Phenolic profiles, antioxidant, and antiproliferative activities of turmeric (Curcuma longa). Ind Crops Prod 152: 112561. DOI: 10.1016/j.indcrop.2020.112561.
Yap VL, Tan LF, Rajagopal M, Wiart C, Selvaraja M, Leong MY, Tan PL. 2023. Evaluation of phytochemicals and antioxidant potential of a new polyherbal formulation TC-16: Additive, synergistic or antagonistic? BMC Complement Med Ther 23: 93. DOI: 10.1186/s12906-023-03921-0.
Yu J, Hu W, Zhang X, 2025. Study on the synthesis, structural characterization and antifungal mechanism of ar-turmerone derivatives. J Mol Struct 1346: 142821. DOI: 10.1016/j.molstruc.2025.142821.
Zhang C, Xie Y, Qiu W, Mei J, Xie J. 2023. Antibacterial and antibiofilm efficacy and mechanism of ginger (Zingiber officinale) essential oil against Shewanella putrefaciens. Plants 12 (8): 1720. DOI: 10.3390/plants12081720.
Zhang S, Kou X, Zhao H, Mak K-K, Balijepalli MK, Pichika MR. 2022. Zingiber officinale var. rubrum: Red ginger’s medicinal uses. Molecules 27 (3): 775. DOI: 10.3390/molecules27030775.