Lactic acid bacteria isolated from the digestive tract of spiny lobsters (Panulirus homarus) and their potency as probiotics
Article Sidebar
Abstract Views: 312
File Views: 74
Main Article Content
Abstract
Abstract. Faturrahman, Auliyah KR, Juanda LA, Setyaningrum TW, Sarkono, Suryadi BF, Azhar F, Priyambodo B, Qoriasmadillah W. 2025. Lactic acid bacteria isolated from the digestive tract of spiny lobsters (Panulirus homarus) and their potency as probiotics. Biodiversitas 26: 1827-1835. Spiny lobsters (Panulirus homarus) are an on-demand marine fishery commodity with significant economic value. However, collected data indicate that the lobsters exhibit low growth rates and high mortality due to disease infection caused by Vibrio spp. The provision of probiotics in feed represents a potential solution to this issue. Lactic acid bacteria (LAB) have been identified as probiotics due to their ability to counteract pathogenic bacteria and synthesize enzymes capable of hydrolyzing complex molecules, such as proteins, carbohydrates, and lipids, thereby promoting organismal growth. The objective of this study was to isolate LAB from the digestive tract of spiny lobsters as probiotic candidates. The selection of LAB isolates from the digestive tract was based on their ability to inhibit the growth of Vibrio harveyi bacteria and their capacity to produce a range of extracellular enzymes, including proteases, lipases, and amylases. The results demonstrated the isolation of seven LAB isolates, classified as Pediococcus, Enterococcus, and Lactobacillus genera, which exhibited the capacity to impede the proliferation of V. harveyi. The majority of the isolated LAB were found to possess the ability to hydrolyze protein, starch, and fat, in addition to exerting inhibitory effects against the growth of V. harveyi. Among the isolates, SP6 exhibited the most promising characteristics as a probiotic candidate by demonstrating robust protein and fat hydrolysis abilities, along with a significant capacity to inhibit the growth of V. harveyi.
Article Details
Issue
Section
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
References
Agustin R, Amin M, Lamid M. 2023. The effect of formulated diets with different protein sources on feed consumption, feed conversion ratio, and nutrient retention of scalloped spiny lobster (Panulirus homarus). IOP Conf Ser: Earth Environ Sci 1273 (1): 012047. DOI: 10.1088/1755-1315/1273/1/012047.
Anee IJ, Alam S, Begum RA, Shahjahan RM, Khandaker AM. 2021. The role of probiotics on animal health and nutrition. J Basic Appl Zool 82: 52. DOI: 10.1186/s41936-021-00250-x.
Axelsson L. 2004. Lactic acid bacteria: Microbiological and functional aspect. Rev Bras Cienc Farm 42 (3). DOI: 10.1590/S1516-93322006000300018.
Cappucino JG, Sherman N. 2002. Microbiology: A Laboratory Manual. 6th Edition, Pearson Education Inc., San Francisco.
Chandra P, Enespa, Singh R, Arora PK. 2020. Microbial lipases and their industrial applications: A comprehensive review. Microb Cell Fact 19 (1): 169. DOI: 10.1186/s12934-020-01428-8.
Chizhayeva A, Amangeldi A, Oleinikova Y, Alybaeva A, Sadanov A. 2022. Lactic acid bacteria as probiotics in sustainable development of aquaculture. Aquat Living Resour 35: 10. DOI: 10.1051/alr/2022011.
Daniels CL, Merrifield DL, Ringø E, Davies SJ. 2013. Probiotic, prebiotic and synbiotic applications for the improvement of larval European lobster (Homarus gammarus) culture. Aquaculture 416-417: 396-406. DOI: 10.1016/j.aquaculture.2013.08.001.
Derunets AS, Selimzyanova AI, Rykov SV, Kuznetsov AE, Berezina OV. 2024. Strategies to enhance stress tolerance in lactic acid bacteria across diverse stress conditions. World J Microbiol Biotechnol 40 (4): 126. DOI: 10.1007/s11274-024-03905-3.
Dida G. 2018. Isolation and characterization of starch degrading rhizobacteria from soil of Jimma University Main Campus, Ethiopia. Afr J Microbiol Res 12 (32): 788-795. DOI: 10.5897/ajmr2018.8873.
El-Haroun ER, Goda AS, Kabir Chowdhury MA. 2006. Effect of dietary probiotic biogen supplementation as a growth promoter on growth performance and feed utilization of Nile tilapia, Oreochromis niloticus (L.). Aquacult Res 37: 1473-1480. DOI: 10.1111/j.1365-2109.2006.01584.x.
Elida M, Agustina A, Ermiati E, Desminarti S. 2022. Isolate characterization and amylolytic properties of lactic acid bacteria from traditional fermented dadih. IOP Conf Ser Earth Environ Sci 1097 (1): 012025. DOI: 10.1088/1755-1315/1097/1/012025.
Erlania E, Radiarta IN, Sugama K. 2014. Dinamika kelimpahan benih lobster (Panulirus spp.) di perairan Teluk Gerupuk, Nusa Tenggara Barat: Tantangan pengembangan teknologi budidaya lobster. Jurnal Riset Akuakultur 9: 475-486. DOI: 10.15578/jra.9.3.2014.475-486. [Indonesian]
Fachri M, Amoah K, Huang Y, Cai J, Alfatat A, Ndandala CB, Shija VM, Jin X, Bissih F, Chen H. 2024. Probiotics and paraprobiotics in aquaculture: A sustainable strategy for enhancing fish growth, health and disease prevention-a review. Front Mar Sci 11: 1499228. DOI: 10.3389/fmars.2024.1499228.
Faturrahman, Ismiati I, Nurhasanah A. 2019. Distribusi bakteri penghasil enzim ekstraseluler pada saluran pencernaan lobster mutiara (Panulirus ornatus). Jurnal Sains Teknologi dan Lingkungan 5 (2): 71-82. DOI: 10.29303/jstl.v5i2.129. [Indonesian]
Fauziah PN, Nurhajati J, Chrysanti. 2015. Daya antibakteri filtrat asam laktat dan bakteriosin Lactobacillus bulgaricus KS1 dalam menghambat pertumbuhan Klebsiella pneumoniae Strain ATCC 700603, CT1538, dan S941. Majalah Kedokteran Bandung 47 (1): 35-41. DOI: 10.15395/mkb.v47n1.395. [Indonesian]
Finanda A, Mukarlina, Rahmawati. 2021. Isolasi dan karakterisasi genus bakteri asam laktat dari fermentasi daging buah pisang kepok (Musa paradisiaca L.). Protobiont 10 (2): 37-41. DOI: 10.26418/protobiont.v10i2.53897. [Indonesian]
Furini G, Berger JS, Campos JA, Sand STVD, Germani JC. 2018. Production of lipolytic enzymes by bacteria isolated from biological effluent treatment systems. An Acad Bras Ciênc 90 (3): 2955-2965. DOI: 10.1590/0001-3765201820170952.
Haliman RW, Kurnia MD, Iman MN, Kusuma SA, Halalludin B, Rizkiantino R, Sari PP, Rahayu M, Fitriana RN, Panjaitan BV, Jacinda AK, Laiman H. 2023. Isolation of Pediococcus pentosaceus to compete Vibrio harveyi in the shrimp Litopenaeus vannamei hatchery. Biodiversitas 24: 4514-4520. DOI: 10.13057/biodiv/d240853.
Hamidah MN, Rianingsih L, Romadhon R. 2019. Aktivitas antibakteri isolat bakteri asam laktat dari peda dengan jenis ikan berbeda terhadap E. coli dan S. aureus. Jurnal Ilmu dan Teknologi Perikanan 1 (2): 11-21. DOI: 10.14710/jitpi.2019.6742. [Indonesian]
Hancz C. 2022. Application of probiotics for environmentally friendly and sustainable aquaculture: A review. Sustainability 14 (22): 15479. DOI: 10.3390/su142215479.
Indira M, Venkateswarulu TC, Abraham Peele K, Nazneen Bobby M, Krupanidhi S. 2019. Bioactive molecules of probiotic bacteria and their mechanism of action: A review. 3 Biotech 9 (8): 306. DOI: 10.1007/s13205-019-1841-2.
Khushboo, Karnwal A, Malik T. 2023. Characterization and selection of probiotic lactic acid bacteria from different dietary sources for development of functional foods. Front Microbiol 14: 1170725. DOI: 10.3389/fmicb.2023.1170725.
Kieliszek M, Pobiega K, Piwowarek K, Kot AM. 2021. Characteristics of the proteolytic enzymes produced by lactic acid bacteria. Molecules 26 (7): 1858. DOI: 10.3390/molecules26071858.
Kivanc M, Acu E. 2022. Production of extracellular lipase by Enterococcus faecium E68 with olive oil waste as substrate. Biotecnia 24 (3): 87-93. DOI: 10.18633/biotecnia.v24i3.1750.
Lein EY, Lal MTM,Maran BAV, Ch’ng CL, Hamasaki K, Sano M, Tuzan AD. 2022. Gastrointestinal microbiota of spiny lobster: A review. Fishes 7 (3): 108. DOI: 10.3390/fishes7030108.
Maharani SD, Amin M, Lamid M. 2023. Potency of formulated diets with different protein on feed consumption, feed conversion ratio, feed efficiency and nutrient retention of scalloped spiny lobster (Panulirus homarus). IOP Conf Ser: Earth Environ Sci 1273 (1): 012075. DOI: 10.1088/1755-1315/1273/1/012075.
Manoharan M, Balasubramanian TS. 2022. An extensive review on production, purification, and bioactive application of different classes of bacteriocin. J Trop Biodivers Biotechnol 7 (3): jtbb72735. DOI: 10.22146/jtbb.72735.
Maryati Y, Nuraida L, Hariyadi RD. 2021. Production of organic acid and Short-Chain Fatty Acids (SCFA) from lactic acid bacteria isolate on oligosaccharide media. Jurnal Kimia Sains Aplikasi 24 (6): 213-221. DOI: 10.14710/jksa.24.6.213-221.
Ma?átková O, Pospíšilová D, Michailidu, Jaroš P, Masák J. 2019. Effect of subinhibitory concentration of antibiotics on Rhodococcus erythropolis and Pseudomonas fluorescens biofilm formation. Chem Pap 73: 1113-1119. DOI: 10.1007/s11696-018-0662-9.
Muñoz-Atienza E, Gómez-Sala B, Araújo C, Campanero C, del Campo R, Hernández PE, Herranz C, Cintas LM. 2013. Antimicrobial activity, antibiotic susceptibility and virulence factors of lactic acid bacteria of aquatic origin intended for use as probiotics in aquaculture. BMC Microbiol 13: 15. DOI: 10.1186/1471-2180-13-15.
Nangin D, Sutrisno A. 2015. Enzim amilase pemecah pati mentah dari mikroba: Kajian pustaka. Jurnal Pangan dan Agroindustri 3 (3): 1032-1039. [Indonesian]
Nguyen VD, Pham TT, Nguyen THX, Nguyen TTX, Hoj L. 2014. Screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (Panulirus ornatus) juveniles. Fish Shellfish Immunol 40: 49-60. DOI: 10.1016/j.fsi.2014.06.017.
O’toole GA. 2016. Classic spotlight: How the gram stain works. J Bacteriol 198 (23): 3128. DOI: 10.1128/jb.00726-16.
Perera E, Simon C. 2015. Digestive physiology of spiny lobsters: Implications for formulated diet development. J Rev Aquacult 7 (4): 243-261. DOI: 10.1111/raq.12066.
Phieter AC, Chrisnasari R, Pantjajani T. 2020. Karakterisasi enzim pemecah pati dari malt serelia. Keluwih: J Sci Technol 1 (1): 38-48. DOI: 10.24123/saintek.v1i1.2773. [Indonesian]
Prastiyanto ME, Wardoyo FA, Wilson W, Darmawati S. 2020. Antibacterial activity of various extracts of Averrhoa bilimbi against multidrug resistant bacteria. Biosaintifika 12 (2): 163-168. DOI: 10.15294/biosaintifika.v12i2.23600.
Rahayu HM, Setiadi AE. 2023. Isolation and characterization of indigenous lactic acid bacteria from Pakatikng Rape, Dayak’s Traditional Fermented. Food Nat Sci Educ Res J 9 (2): 920-925. DOI: 10.29303/jppipa.v9i2.2801.
Rahayu S, Amoah K, Huang Y, Cai J, Wang B, Shija VM, Jin X, Anokyewaa MA, Jiang M. 2024. Probiotics application in aquaculture: Its potential effects, current status in China and future prospects. Front Mar Sci 11: 1455905. DOI: 10.3389/fmars.2024.1455905.
Riadi S, Setiyawati D, Situmeang S. 2020. Isolasi dan uji potensi bakteri asam laktat asal kimchii dan teh kombucha dalam menghambat bakteri patogen. Jurnal Kesmas Prima Indonesia 4 (1): 25-29. DOI: 10.34012/jkpi.v2i1.891. [Indonesian]
Ringø E, Doan HV, Lee S, Song SK. 2020. Lactic acid bacteria in shellfish: possibilities and challenges. Rev Fish Sci Aquac 28 (2): 139-169. DOI: 10.1080/23308249.2019.1683151.
Ringø E, Hoseinifar SH, Ghosh K, Doan HV, Beck BR, Song SK. 2018. Lactic acid bacteria in finfish-An update. Front Microbiol 9: 1818. DOI: 10.3389/fmicb.2018.01818.
Ruiz Rodríguez LG, Mohamed F, Bleckwedel J, Medina R, Vuyst LD, Hebert EM, Mozzi F. 2019. Diversity and functional properties of lactic acid bacteria isolated from wild fruits and flowers present in Northern Argentina. Front Microbiol 10: 1091. DOI: 10.3389/fmicb.2019.01091.
Rumampuk YB, Wowor PM, Mambo CD. 2017. Uji daya hambat ekstrak spons laut (Callyspongia aerizusa) terhadap pertumbuhan bakteri Salmonella typhi dan Streptococcus pyogenes. eBiomedik 5 (2). DOI: 10.35790/ebm.5.2.2017.18480. [Indonesian]
Sandi FM, Subagiyo S. 2022. Aktifitas antibakteri isolat bakteri asam laktat saluran pencernaan kuda laut (Hippocampus kuda Bleeker, 1852) terhadap Vibrio harvey. Jurnal Kelautan Tropis 25 (2): 241-248. [Indonesian]
Sari M. 2018. Kemampuan antimikroba bakteri asam laktat (BAL) dari bekasam dalam menghambat Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella sp. [Thesis]. Universitas Sumatera Utara, Medan. [Indonesian]
Silitonga LR, Nursyirwani, Effendi I. 2019. Isolation, identification and sensivity of amilolitic bacteria from mangrove ecosystem sediment in Purnama Marine Station Dumai on the phatogenic bacteria. Asian J Aquat Sci 2 (3): 257-266. DOI: 10.31258/ajoas.2.3.257-266.
Suciati P, Tjahjaningsih W, Masithah ED, Pramono H. 2016. Activity enzymatic of isolate lactic acid bacteria from the digestive tract of mud crab (Scylla spp.) as a candidate probiotics. Jurnal Ilmiah Perikanan Kelautan 8 (2): 94-108. DOI: 10.20473/jipk.v8i2.11182.
Sumarsih S, Sulistiyanto B, Sutrisno CI, Rahayu ES. 2012. Peran probiotik bakteri asam laktat terhadap produktivitas unggas. Jurnal Litbang Provinsi Jawa Tengah 10 (1): 1-9. [Indonesian]
Thierry A, Collins YF, Mukdsi MCA, McSweeney PLH, Wilkinson MG, Spinnler HE. 2017. Lipolysis and metabolism of fatty acids in cheese. In: McSweeney PLH, Fox PF, Cotter PD, Everett DW (eds). Cheese. Academic Press, London. DOI: 10.1016/B978-0-12-417012-4.00017-X.
Torres-Maravilla E, Parra M, Maisey K, Vargas RA, Cabezas-Cruz A, Gonzalez A, Tello M, Bermúdez-Humarán LG. 2024. Importance of probiotics in fish aquaculture: Towards the identification and design of novel probiotics. Microorganisms 12 (3): 626. DOI: 10.3390/microorganisms12030626.
Truc LNT, Ngoc AT, Hong TTT, Thanh TN, Kim HH, Kim LP, Truong GH, Quoc PT, Ngoc TNT et al. 2019. Selection of lactic acid bacteria (LAB) antagonizing Vibrio parahaemolyticus: The pathogen of acute hepatopancreatic necrosis disease (AHPND) in whiteleg shrimp (Penaeus vannamei). Biology 8: 91. DOI: 10.3390/biology8040091.
Valente CS, Wan AH. 2021. Vibrio and major commercially important vibriosis diseases in decapod crustaceans. J Invertebr Pathol 181: 107527. DOI: 10.1016/j.jip.2020.107527.
Veronica N, Liew CV, Heng PWS. 2022. Impact of amylose-amylopectin ratio of starches on the mechanical strength and stability of acetylsalicylic acid tablets. AAPS PharmSciTech 23 (5): 118. DOI: 10.1208/s12249-022-02266-0.
Wang Y, Wu J, Lv M, Shao Z, Hungwe M, Wang J, Bai X, Xie J, Wang Y, Geng W. 2021. Metabolism characteristics of lactic acid bacteria and the expanding applications in food industry. Front Bioeng Biotechnol 9: 612285. DOI: 10.3389/fbioe.2021.612285.
Wei C, Luo K, Wang M, Li Y, Pan M, Xie Y, Tian X. 2022. Evaluation of potential probiotic properties of a strain of Lactobacillus plantarum for shrimp farming: From beneficial functions to safety assessment. Front Microbiol 13: 854131. DOI: 10.3389/fmicb.2022.854131.
Wihartati LD, Permana IDGM, Hapsari NMI, Puspawati NN. 2022. Antibacterial activity of Lactobacillus plantarum 1 RN9 against Escherichia coli ATCC 25922. J Itepa 11 (4): 669-687. DOI: 10.24843/itepa.2022.v11.i04.p08.
Wu D, Dai M, Shi Y, Zhou Q, Li P, Gu Q. 2022. Purification and characterization of bacteriocin produced by a strain of Lacticaseibacillus rhamnosus ZFM216. Front Microbiol 13: 1050807. DOI: 10.3389/fmicb.2022.1050807.
Zhang F, Zhou K, Xie F, Zhao Q. 2022. Screening and identification of lactic acid bacteria with antimicrobial abilities for aquaculture pathogens in vitro. Arch Microbiol 204 (12): 689. DOI: 10.1007/s00203-022-03285-y.