Abundance of fungi on rice straw compost and husk biochars by in-vitro heavy metals mycosorbent

Main Article Content

NURUL PUSPITA PALUPI
RORO KESUMANINGWATI
ARYA BAYA SRIPATI
ESTI HANDAYANI HARDI
FAHRUNSYAH
DWI ERMAWATI RAHAYU
RUDY AGUNG NUGROHO
SURYA DARMA
SURIA DARMA IDRIS

Abstract

Abstract. Palupi NP, Kesumaningwati R, Sripati AB, Hardi EH, Fahrunsyah, Rahayu DE, Nugroho RA, Darma S, Idris SD. 2025. Abundance of fungi on rice straw compost and husk biochars by in-vitro heavy metals mycosorbent. Biodiversitas 26: 1983-1990. In Samarinda, all paddy fields are contaminated by heavy metals with concentrations exceeding the critical threshold and has disrupted human health and worsened the quality of the agroecosystem of rice fields. This study aims to evaluate the effectiveness of compost and biochar application in absorbing heavy metals in paddy field soil in Samarinda. The research method involves the application of compost, biochar, and their combination on paddy field soil contaminated with heavy metals using incubation experiment with 5 treatments and 4 replications, completely randomized design, and measured parameters (pH, Fe, Zn Cd, and Aspergillus niger). The results indicate that the use of compost significantly increases the population of A. niger and reduces the concentration of heavy metals such as Fe, Zn, and Cd in the soil. On the other hand, biochar has also proven effective in reducing the content of heavy metals, supported by its high adsorption capacity. The combination of compost and biochar shows better results compared to individual applications, indicating synergy between organic nutrients and adsorption capabilities. The correlation between the population of A. niger and heavy metals demonstrates a significant relationship, confirming the role of microbes in soil bioremediation. The practical implication of this research underscores the importance of organic fertilizer use in reducing heavy metal contamination, supporting sustainable agriculture, and reducing dependence on synthetic chemicals. Future research is recommended to further understand the mechanisms of bioremediation and soil microbe interactions in this context. In conclusion, the application of compost and biochar offers a holistic approach to effectively address heavy metal contamination issues, enhance soil quality, and support environmentally friendly agriculture.

Article Details

Section

Articles

References

Alengebawy A, Abdelkhalek ST, Qureshi SR, and Wang M. 2021. Heavy Metals and Pesticides Toxicity in Agricultural Soil and Plants: Ecological Risks and Human Health Implications. Toxics, 9(3), 42; https://doi.org/10.3390/toxics9030042.

Uddin, M. M., Zakeel, M. C. M., Zavahir, J. S., Marikar, F. M. M. T., & Jahan, I. (2021). Heavy Metal Accumulation in Rice and Aquatic Plants Used as Human Food: A General Review. Toxics, 9(12), 360. https://doi.org/10.3390/toxics9120360

Lin C, Cheruiyot N.K, Bui XT and Ngo HH. 2022. Composting and its application in bioremediation of organic contaminants. Pages 1073-1089. https://doi.org/10.1080/21655979.2021.2017624

Ayilara, M., Olanrewaju, O., Babalola, O., & Odeyemi, O. (2020). Waste Management through Composting: Challenges and Potentials. Sustainability, 12(11), 4456. https://doi.org/10.3390/su12114456.

Wedayani, N. M., Rai, I. N., Mahardika, I. G., & Wijana, I. M. S. (2024). Pengaruh Pemberian Biochar Limbah Pisang terhadap Kesuburan Tanah. Agro Bali: Agricultural Journal, 7(1), 137-145.

Nugraha, F. A., Kirmi, H., Haryanto, B., & Afiffa, M. (2020). Analisis penggunaan media tandan sawit dan kompos dengan sistem aerobic wetland dalam mengolah air asam tambang. SPECTA Journal of Technology, 4(2), 35-44.

Singh M, Singh S, Rai PK, Suyal DC, Saurabh S, Soni R, Giri K dan Yadav AN. 2021. Fungi in Remediation of Hazardous Wastes: Current Status and Future Outlook. pp 195–224. Recent Trends in Mycological Research. Part of the book series: Fungal Biology ((FUNGBIO)).

Bikash Chandra Behera BC. 2020. Citric acid from Aspergillus niger: a comprehensive overview. Pages 727-749. https://doi.org/10.1080/1040841X.2020.1828815

Suhastyo, A. A., Anas, I., Santosa, D. A., & Lestari, Y. (2013). Studi mikrobiologi dan sifat kimia mikroorganisme lokal (MOL) yang digunakan pada budidaya padi metode SRI (System of Rice Intensification). Sainteks, 10(2).

Agnieszka Medy?ska-Juraszek, Magdalena Bednik, and Piotr Chohura. 2020. Assessing the Influence of Compost and Biochar Amendments on the Mobility and Uptake of Heavy Metals by Green Leafy Vegetables. Int. J. Environ. Res. Public Health 2020, 17(21), 7861; https://doi.org/10.3390/ijerph17217861. https://www.mdpi.com/1660-4601/17/21/7861.

Gao J, Han H, Gao C, Wang Y, Dong B, Xu Z. 2023. Organic amendments for in situ immobilization of heavy metals in soil: A review. Chemosphere, Volume 335. https://doi.org/10.1016/j.chemosphere.2023.139088.

Sakhiya AK, Anand A dan Kaushal P. 2020. Production, activation, and applications of biochar in recent times. Review. volume 2, pages 253–285. https://link.springer.com/article/10.1007/s42773-020-00047-1.

Haider FU, Coulter JA, Cai L, Hussain S, Cheema SA, Wu J, dan Zhang R. An overview on biochar production, its implications, and mechanisms of biochar-induced amelioration of soil and plant characteristics. Pedosphere. Volume 32, Pages 107-130 https://doi.org/10.1016/S1002-0160(20)60094-7.

Wang M, Wu Y, Zhao J, Liu Y, Gao L, Jiang Z, Zhang J, dan Tian W. 2022. Comparison of composting factors, heavy metal immobilization, and microbial activity after biochar or lime application in straw-manure composting. Bioresource Technology. Volume 363. https://doi.org/10.1016/j.biortech.2022.127872.

Wang L, Guan H. Hu J, Feng Y, Li X, Yusef KK, , Gao H., and Da Tian*. Aspergillus niger Enhances Organic and Inorganic Phosphorus Release from Wheat Straw by Secretion of Degrading Enzymes and Oxalic Acid. J. Agric. Food Chem. 2022, 70, 35, 10738–10746. https://doi.org/10.1021/acs.jafc.2c03063. American Chemical Society.

Chaurasia PK, Nagraj, Sharma N, Kumari S, Yadav M, Singh S, Mani A, Yadava S, and Bharati. SL Biotechnology and Bioengineering. https://doi.org/10.1002/bit.28268

Priyadarshini E, Priyadarshini SS, Cousins BG c, Pradhan N. Metal-Fungus interaction: Review on cellular processes underlying heavy metal detoxification and synthesis of metal nanoparticles. Chemosphere. Volume 274, https://doi.org/10.1016/j.chemosphere.2021.129976.

Allen V. Barker and Gretchen M. Bryson. 2020. Bioremediation of Heavy Metals and Organic Toxicants by Composting. Mini-Review Article. Open Access. Volume 2. Article ID 937025 https://doi.org/10.1100/tsw.2002.91. https://www.hindawi.com/journals/tswj/2002/937025/

L. Wang, Y.J. Wang, F. Ma, V. Tankpa, S.S. Bai, X.M. Guo, X. Wang. 2019. Mechanisms and reutilization of modified biochar used for removal of heavy metals from wastewater: A review. Science of The Total Environment. Volume 668, 10 June 2019, Pages 1298-1309. https://doi.org/10.1016/j.scitotenv.2019.03.011.

Li Y, Yu H, Liu L, and Yu H. 2021. Application of co-pyrolysis biochar for the adsorption and immobilization of heavy metals in contaminated environmental substrates. Journal of Hazardous Materials. Volume 420. https://doi.org/10.1016/j.jhazmat.2021.126655

Masooma Batool M, Waqas-ud-Din Khan W, Hamid Y, Farooq MA, Naeem MA d, and Nadeem F. Interaction of pristine and mineral engineered biochar with microbial community in attenuating the heavy metals toxicity: A review. Applied Soil Ecology. Volume 175, July 2022, 104444. https://doi.org/10.1016/j.apsoil.2022.104444

Qiu, B., Shao, Q., Shi, J., Yang, C., & Chu, H. 2022. Application of biochar for the adsorption of organic pollutants from wastewater: Modification strategies, mechanisms and challenges. Separation and Purification Technology, 300, 121925. https://doi.org/10.1016/j.seppur.2022.121925

Song, B., Almatrafi, E., Tan, X., Luo, S., Xiong, W., Zhou, C., Qin, M., Liu, Y., Cheng, M., Zeng, G., & Gong, J. (2022). Biochar-based agricultural soil management: An application-dependent strategy for contributing to carbon neutrality. Renewable and Sustainable Energy Reviews, 164, 112529. https://doi.org/10.1016/j.rser.2022.112529

Sharma GK, Jena RK, Hota S, Kumar A, Ray P, Fagodiya RK, Malav LC, Yadav KK, Gupta DK, Khan SA and Ray SK. Recent Development in Bioremediation of Soil Pollutants Through Biochar for Environmental Sustainability. Chapter. Biochar Applications in Agriculture and Environment Management.

Talukdar D, Jasrotia T, Sharma R, Jaglan S d, Kumar R, Vats R, Kumar R, Mahnashi MH, and Umar A. Evaluation of novel indigenous fungal consortium for enhanced bioremediation of heavy metals from contaminated sites. Environmental Technology & Innovation. Volume 20, November 2020, 101050. htps://doi.org/10.1016/j.eti.2020.101050.

Zakaria Z, Zulkafflee NS, Redzuan NAM, Selamat J, Ismail MR, Praveena SM, Tóth G and Razis AHF. Understanding Potential Heavy Metal Contamination, Absorption, Translocation and Accumulation in Rice and Human Health Risks. Plants 2021, 10(6), 1070; https://doi.org/10.3390/plants10061070

Sharma P and Kumar S. Bioremediation of heavy metals from industrial effluents by endophytes and their metabolic activity: Recent advances. Bioresource Technology. Volume 339, November 2021, 125589. https://doi.org/10.1016/j.biortech.2021.125589

El-Mahdy OM and Mohamed HI. Biosorption effect of Aspergillus niger and Penicillium chrysosporium for Cd- and Pb-contaminated soil and their physiological effects on Vicia faba L. https://link.springer.com/journal/11356. Volume 28, pages 67608–67631. Environmental Science and Pollution Research.

Pathak A, Kothari R, Vinoba M, Habibi N, and Tyagi VV. Fungal bioleaching of metals from refinery spent catalysts: A critical review of current research, challenges, and future directions. Journal of Environmental Management. Volume 280, 15 February 2021, 111789. https://doi.org/10.1016/j.jenvman.2020.111789

Goutam J, Sharma J, Singh R and Sharma D. Fungal-Mediated Bioremediation of Heavy Metal–Polluted Environment. Chapter. Microbial Rejuvenation of Polluted Environment. Part of the book series: Microorganisms for Sustainability ((MICRO,volume 26))

Kumar V and Dwivedi SK. Mycoremediation of heavy metals: processes, mechanisms, and affecting factors. Environmental Science and Pollution Research. Volume 28, pages 10375–10412, (2021)

Qiu J, Song X, Li S, Zhu B, Chen Y, Zhang L and Li Z. Experimental and modeling studies of competitive Pb (II) and Cd (II) bioaccumulation by Aspergillus niger. Environmental Biotechnology. Volume 105, pages 6477–6488, (2021). Applied Microbiology and Biotechnology.

Cabrera-Barjas G, Gallardo F, Nesic A, Taboada E, Marican A, Mirabal-Gallardo Y, Avila-Salas F, Delgado N, de Armas-Ricard M, and Valdes O. Utilization of industrial by-product fungal biomass from Aspergillus niger and Fusarium culmorum to obtain biosorbents for removal of pesticide and metal ions from aqueous solutions. Journal of Environmental Chemical Engineering. Volume 8, Issue 5, October 2020, 104355. https://doi.org/10.1016/j.jece.2020.104355.

Pan X, Zhang S, Zhong Q, Gong G, Wang G, Guo X, and Xu X Effects of soil chemical properties and fractions of Pb, Cd, and Zn on bacterial and fungal communities. Science of The Total Environment. Volume 715, 1 May 2020, 136904. https://doi.org/10.1016/j.scitotenv.2020.136904.

Dusengemungu L, Kasali G, Gwanama C, dan Mubemba B. Overview of fungal bioleaching of metals. Environmental Advances. Volume 5, October 2021, 100083. https://doi.org/10.1016/j.envadv.2021.100083.

Wu Y, Li X, Yu L, Wang T, JWang J, T and Liu T. 2022. Review of soil heavy metal pollution in China: Spatial distribution, primary sources, and remediation alternatives. Resources, Conservation and Recycling. Volume 181, June 2022, 106261. https://doi.org/10.1016/j.resconrec.2022.106261. https://www.sciencedirect.com/science/article/abs/pii/S0921344922001094.

Most read articles by the same author(s)