Barcoding of ornamental freshwater shrimp, Neocaridina denticulata (De Haan, 1844) from the aquatic ornamental market in Purbalingga, Central Java, Indonesia




Abstract. Kusmintarsih ES, Mahmoud HHA, Sastranegara MH, Syakuri H,Nuryanto A, Ambarningrum TB. 2023. Barcoding of ornamental freshwater shrimp, Neocaridina denticulata (De Haan, 1844) from the aquatic ornamental market in Purbalingga, Central Java, IndonesiaBiodiversitas 243601-3608Indonesia is one of the world’s leading aquatic ornamental shrimps producers and exports. One of the aquatic ornament shrimp species is a Neocaridina denticulate (De Haan, 1844). There is a wide range of colors including red, bright red, yellow, orange, green, blue, violet, black, and color combinations. It is a landlocked Atydiae family species comprising31 species and subspecies. Due to taxonomic difficulties, this species is continually under revision, and the validation of a few species is currently questionable and uncertain for the relationships among the various colors of N. denticulata. The Cytochrome Oxidase gene I (CO1) gene sequences of mitochondrial DNA were used for species DNA barcoding studies. This study aimed to validate the taxonomic status of different colors of N. denticulata species using the mitochondrial DNA or mtDNA Cytochrome oxidase (CO1) gene sequences. Neighbour Joining trees were constructed based on 545 bp of CO1 gene from white, black, red, yellow, blue colors, and their combination. Several sequences were derived from GenBank for relevant species from different countries and the outgroup. The result showed that all samples are homolog, even to N. denticulata from China NC_023823.1.


Bouvier, E.L., 1904. Crevettes de la famille des Atyidés: espèces qui font partie des collections du Muséum d’Histoire Naturelle. Bulletin du Muséum d’Histoire naturelle, 10, pp.129–138.
Cai, Y. & Duan, Y., 1998. Caridina lufengensis, a new species of shrimp (Crustacea: Decapoda: Atyidae) from Yunnan, South-western China. Raffles Bulletin of Zoology, 46(2), pp.329–334.
Christodoulou, M. et al., 2012. Revision of the freshwater genus Atyaephyra (Crustacea, Decapoda, Atyidae) based on morphological and molecular data. ZooKeys, 229, pp.53–110. doi: 10.3897/zookeys.229.3919.
De Grave, S. & Fransen, C., 2013. Carideorum Catalogus?: The Recent Species of the Dendrobranchiate , Stenopodidean , Procarididean and Caridean Shrimps ( Crustacea?: Decapoda ) Fossil taxa How many shrimp species are there??,
Englund, R.A. & Cai, Y., 1999. The occurrence and description of Necaridina denticulata sinensis (Kemp, 1918) (Crustacea: Decapoda: Atyidae), a new introduction to the Hawaiian Islands. Bishop Museum Occasional Papers, 58(August), pp.58–65.
Gamit, A.B. et al., 2018. A Report of Centrocestus formosanus (Nishigori, 1924) (Digenea: Heterophyidae) in Intermediate Host (Fish). International Journal of Current Microbiology and Applied Sciences, 7(07), pp.928–936. doi: 10.20546/ijcmas.2018.707.112.
Hebert, P.D.N. et al., 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512), pp.313–321. doi: 10.1098/rspb.2002.2218.
Hung, M. & Chan, T.-Y., 1993. Atyid Shrimps ( Decapoda?: Caridea ) of Taiwan ,. , 13(3), pp.481–503.
Imtiaz, A., Mohd Nor, S.A. & Md. Naim, D., 2017. Progress and potential of DNA barcoding for species identification of fish species. Biodiversitas, 18(4), pp.1394–1405. doi: 10.13057/biodiv/d180415.
Island, F. et al., 2021. ???? 1 ????? 2 ??? ? 3 ????? 4. , 71, pp.63–71.
Jablo?ska, A. et al., 2018. First record and DNA barcodes of the aquarium shrimp, Neocaridina davidi, in Central Europe from thermally polluted River Oder canal, Poland. Knowledge and Management of Aquatic Ecosystems, 2018-Janua(419). doi: 10.1051/kmae/2018004.
Klotz, W. et al., 2013. Two Asian fresh water shrimp species found in a thermally polluted stream system in north Rhine-Westphalia, Germany. Aquatic Invasions, 8(3), pp.333–339. doi: 10.3391/ai.2013.8.3.09.
Levitt-Barmats, Y. et al., 2019. Life-history traits and ecological characteristics of the ornamental shrimp Neocaridina denticulata (De Haan, 1844), recently introduced into the freshwater systems of Israel. Aquatic Invasions, 14(4), pp.684–702. doi: 10.3391/ai.2019.14.4.08.
Liang, X.G., Yan, S.L. & Wang, X.Y., 1984. A new species of Caridina (Decapoda: Caridea) from Yunnan, China [in Chinese]. Acta Zootaxonomica Sinica, 9(July), pp.253–256.
Niwa, N. et al., 2005. The first record of the ectosymbiotic branchiobdellidan Holtodrilus truncatus (Annelida, Clitellata) and on the freshwater shrimp Neocaridina denticulata denticulata (Caridea, Atyidae) in Japan. Fisheries Science, 71(3), pp.685–687. doi: 10.1111/j.1444-2906.2005.01017.x.
Nur, F.A.H. & Christianus, A., 2013. Breeding and life cycle of Neocaridina denticulata sinensis (Kemp, 1918). Asian Journal of Animal and Veterinary Advances, 8(1), pp.108–115. doi: 10.3923/ajava.2013.108.115.
Pantaleão, J.A.F. et al., 2015. Nutritional vulnerability in early stages of the freshwater ornamental “red cherry shrimp” Neocaridina davidi(Bouvier, 1904) (Caridea: Atyidae). Journal of Crustacean Biology, 35(5), pp.676–681. doi: 10.1163/1937240X-00002357.
Pantaleão, J.A.F. et al., 2017. Post-hatching development of the ornamental ‘Red Cherry Shrimp’ Neocaridina davidi (Bouvier, 1904) (Crustacea, Caridea, Atyidae) under laboratorial conditions. Aquaculture Research, 48(2), pp.553–569. doi: 10.1111/are.12903.
Patoka, J. et al., 2016. Aquarium hitchhikers: attached commensals imported with freshwater shrimps via the pet trade. Biological Invasions, 18(2), pp.457–461. doi: 10.1007/s10530-015-1018-9.
Persis, M., 2009. Chandra Sekhar Reddy A, Rao LM, Khedkar GD, Ravinder K, Nasruddin K (2009) COI (cytochrome oxidase-I) sequence based studies of Carangid fishes from Kakinada coast, India. Mol Biol Rep, 36(7), pp.1733–1740.
Shih, H.-T. & Cai, Y., 2007. Two new species of the land-locked freshwater shrimps genus, Neocaridina Kubo, 1938 (Decapoda: Caridea: Atyidae), from Taiwan, with notes on speciation on the island. Zoological Studies, 46(6), pp.680–694.
Shih, H. Te et al., 2017. A new species of land-locked freshwater shrimp of the genus Neocaridina (Decapoda: Caridea: Atyidae) from Iki Island, Kyushu, Japan. Zoological Studies, 56, pp.1–14. doi: 10.6620/ZS.2017.56-30.
Tahri, M. et al., 2016. Health status of the swim bladder of the European eel Anguilla anguilla in northeastern Algeria’s Lake Oubeïra. ~ 364 ~ International Journal of Fisheries and Aquatic Studies, 4(1), pp.364–369.
Tropea, C., Stumpf, L. & Greco, L.S.L., 2015. Effect of temperature on biochemical composition, growth and reproduction of the ornamental red cherry shrimp Neocaridina heteropoda heteropoda (Decapoda, Caridea). PLoS ONE, 10(3), pp.1–14. doi: 10.1371/journal.pone.0119468.
Van de, N. & Hoa Le, T., 2011. Human infections of fish-borne trematodes in Vietnam: Prevalence and molecular specific identification at an endemic commune in Nam Dinh province. Experimental Parasitology, 129(4), pp.355–361. doi: 10.1016/j.exppara.2011.09.005.
Weiperth, A. et al., 2019. Occurrence of non-native red cherry shrimp in European temperate waterbodies: a case study from Hungary. Knowledge and Management of Aquatic Ecosystems, 2019-Janua(420). doi: 10.1051/kmae/2019002.
Yixiong, C., 1996. A revision of the genus neocaridina(crustacea:decapoda:atyidae). Dong wu fen lei xue bao = Acta zootaxonomica Sinica, 21(2), pp.129–160.

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