Cytotoxic screening of endophytic fungi associated with Catharanthus roseus

##plugins.themes.bootstrap3.article.main##

CATERINA SISKADEWI LAY
IKRA AGUSTINA
PUJI ASTUTI
TRIANA HERTIANI

Abstract

Abstract. Lay CS, Agustina I, Astuti P, Hertiani T. 2023. Cytotoxic screening of endophytic fungi associated with Catharanthus roseus. Biodiversitas 24: 2716-2722. Despite the advancements in cancer therapy, there is still a need for new anticancer agents. Due to the tremendous range of chemical compounds that endophytic fungi create, they have become a crucial component of the current drug development process. The aim of this study was to evaluate the cytotoxic potential of endophytic fungi associated with Catharanthus roseus (L.) G.Don collected from Yogyakarta, Indonesia. All fungal EtOAc extracts were tested against three human cancer cell lines, HeLa, T47D, MCF-7, and Vero cell lines. The potential fungal isolate was identified using molecular techniques. The results showed that a total of seven endophytic fungi were obtained from the different parts of C. roseus. Only one fungus (SCR 3) showed the best cytotoxic activitiy towards all three cell lines used, especially the MCF-7 cell line (IC50 130.90 ?g/mL, Selectivity Index=11.42). The findings of using LC-HRMS also revealed several exciting compounds such as genistein and D-(-)-Mannitol. The results showed that EtOAc extract of SCR 3 (Aspergillus sp.) induced apoptosis but did not affect the cell cycle. This study suggests that EtOAc extract of SCR 3 may be a potential source of secondary metabolites that can function as a new source of anticancer agents.

##plugins.themes.bootstrap3.article.details##

References
Aly AH, Debbab A, Kjer J, Proksch P. 2010. Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products. Fungal Diversity 41: 1-16. DOI: 10.1007/s13225-010-0034-4.
Asai T, Luo D, Yamashita K, Oshima Y. 2013. Structures and biomimetic synthesis of novel ?-Pyrone polyketides of an endophytic Penicillium sp. in Catharanthus roseus. Org. Lett. 15:1020-3. DOI: 10.1021/ol303506t.
Astuti P, Utami E, Nugrahani AW, Sudjadi S. 2012. Genistein abrogates G2 arrest induced by curcumin In p53 deficient
T47D cells. J. Pharm. Sci. 20 (1): 82. DOI: 10.1186/2008-2231-20-82.
Astuti P, Wahyono, Nuryastuti T, Purwantini I, Purwanto. 2014. Antimicrobial and Cytotoxic Activities of Endophytic Fungi Isolated from Artemisia annua L. J. Appl. Pharma Sci 4(10): 047-050. DOI: 10.7324/JAPS.2014.401009.
Corteselli EM, Gibbs-Flournoy E, Simmons SO, Bromberg P, Gold A, Samet JM. 2019. Long chain lipid hydroperoxides increase the glutathione redox potential through glutathione peroxidase 4. Biochim Biophys Acta Gen Subj. 1863(5):950-959. DOI: 10.1016/j.bbagen.2019.03.002.
Das S, Sharangi AB. 2017. Madagascar periwinkle (Catharanthus roseus L.): Diverse medicinal and therapeutic benefits to humankind. J. Pharmacogn. Phytochem. 6:1695-701.
Deshmukh SK, Dufossé L, Chhipa H, Saxena S, Mahajan GB, Gupta MK. 2022. Fungal Endophytes: A Potential Source of Antibacterial Compounds. J. Fungi 8:164. DOI: 10.3390/jof8020164.
Dhayanithy G, Subban K, Chelliah J. 2019. Diversity and biological activities of endophytic fungi associated with Catharanthus roseus. BMC Microbiology 19:22. DOI: 10.1186/s12866-019-1386-x.
Jeon HJ, Choi DK, Choi J, Lee S, Lee H, Yu JH, Min SH. 2021. D-Mannitol Induces a Brown Fat-like Phenotype via a ?3-Adrenergic Receptor-Dependent Mechanism. Cells 10(4):768. DOI: 10.3390/cells10040768.
Jiang H, Fan J, Cheng L, Hu P, Liu R. 2018. The anticancer activity of genistein is increased in estrogen receptor beta 1-positive breast cancer cells. OncoTargets Ther. 11:8153-8163. DOI: 10.2147/OTT.S182239.
Jinu MV, Jayabaskaran C. 2015. Diversity and anticancer activity of endophytic fungi associated with the medicinal plant Saracaasoca. Curr. Environmental & Appl Mycology 5(3), 169-179. DOI:10.5943/ream/5/3/2.
Kharwar RN, Verma VC, Strobel G, Ezra D. 2008. The endophytic fungal complex of Catharanthus roseus (L.) G. Don. Curr. Sci. 8: 228-33.
Kumar A, Patil D, Rajamohanan PR, Ahmad A. 2013. Isolation, purification and characterization of vinblastine and vincristine from endophytic fungus Fusarium oxysporum isolated from Catharanthus roseus. PLoS One 8:e71805. DOI: 10.1371/journal.pone.0071805.
Kusari S, Hertweck C, Spiteller M. 2012. Chemical ecology of endophytic fungi: origins of secondary metabolites. Chem. Biol. 19:792-8. DOI: 10.1016/j.chembiol.2012.06.004.
Ola ARB, Lapailaka T, Wogo HE, Henuk JBD, Simamora A, Mukkun L, Proksch P, Pham CD. 2021. Bioactive Secondary Metabolites from the Mangrove Endophytic Fungi Nigrospora oryzae. Indones. J. Chem. 21 (4), 1016-1022. DOI: 10.22146/ijc.63129.
Ola ARB, Soa CAP, Da Cunha T, Sugi Y, Belli LLH, Lalel HJD. 2020. Antimicrobial metabolite from the endophytic fungi Aspergillus flavus isolated from Sonneratia alba, a mangrove plant of Timor-Indonesia. Rasayan J. Chem. 13(1), 377-381. DOI: 10.31788/RJC.2020.1315585.
Palem PP, Kuriakose GC, Jayabaskaran C. 2016. An endophytic fungus, Talaromycesradicus, isolated from Catharanthus roseus, produces vincristine and vinblastine, which induce apoptotic cell death. PLoS One 10:e0144476. DOI: 10.1371/journal.pone.0144476.
Rai N, Keshri PK, Verma A, Kamble SC, Mishra P, Barik S, Singh SK, Gautam V. 2021. Plant associated fungal endophytes as a source of natural bioactive compounds. Mycology 12(3):139-159. DOI: 10.1080/21501203.2020.1870579.
Sucharitha A, Palempalli, Uma MD, Devi M. 2010. Antimicrobial properties of chilli lipoxygenase products. African J. Microbiology Res. 4(9) 748-752. DOI: 10.5897/AJMR.9000084.
Tamura K, Dudley J, Nei M, Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis (MEGA) software version 6.0. Mol. Biol and Evol. 30: 2725-2729. DOI: 10.1093/molbev/mst197.
Wen J, Okyere SK, Wang S,Wang J, Xie L, Ran Y, Hu Y. 2022. Endophytic Fungi: An Effective Alternative Source of Plant-Derived Bioactive Compounds for Pharmacological Studies. J. Fungi 8: 205. DOI: 10.3390/jof8020205.
Zheng R, Li S, Zhang X, Zhao C. 2021. Biological Activities of Some New Secondary Metabolites Isolated from Endophytic Fungi: A Review Study. Int. J. Mol. Sci. 22: 959. DOI: 10.3390/ijms22020959.

Most read articles by the same author(s)