Antibiotic resistance of emerging pathogenic bacteria of hybrid grouper farming in Indonesia
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Istiqomah I, Isnansetyo A, Murwantoko, Handayani DP, Lestari YN, Taslihan A, Permana IGN, Wijayanti E. 2023. Antibiotic resistance of emerging pathogenic bacteria of hybrid grouper farming in Indonesia. Biodiversitas 24: 2493-2501. A hybrid grouper is among the main coastal farming species in Asian countries, yet vibriosis is still a significant issue. Therefore, a knowledge of pathogenic strains responsible for recent cases is essential for developing appropriate control measures. The purpose of the study was to describe emerging bacterial pathogens associated with hemorrhagic disorder, skin wounds, and mortality in cantang hybrid grouper farms in Indonesia. A total of 30 bacterial strains were recovered from the kidney of diseased grouper on TCBS agar. Based on the ability to induce clinical signs and mortality of grouper juveniles owing to intraperitoneal bacterial infection at 106 CFU/fish, the bacteria were divided into three groups: pathogenic (n=11), low-pathogenic (n=2), and non-pathogenic (n=17). The pathogenic strains were putatively identified as Photobacterium damselae subsp. Damselae (n=8), Vibrio alginolyticus (n=1), Vibrio harveyi (n=2), and Vibrio azureus (n=2) based on the biochemical characteristics and 16S rRNA and DNA gyrase B subunit gene sequences. The pathogenic bacteria were sensitive to erythromycin, gentamycin, oxytetracycline, and kanamycin. However, it still has various resistant patterns to ampicillin, fosfomycin, chloramphenicol, streptomycin, rifampicin, and enrofloxacin. This study demonstrated the emergence of pathogenic P. damselae subsp. damselae, Vibrio alginolyticus, V. harveyi, and V. azureus in grouper aquaculture in Indonesia with multiple antibiotic resistance, which needed to further studies.
##plugins.themes.bootstrap3.article.details##
Most read articles by the same author(s)
- IKA FIRAWATI, MURWANTOKO MURWANTOKO, EKO SETYOBUDI, Morphological and molecular characterization of hairtail (Trichiurus spp.) from the Indian Ocean, southern coast of East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 18 No. 1 (2017)
- FADILAH NOR LAILI LUTFIA, ALIM ISNANSETYO, RATNA ASMAH SUSIDARTI, MUHAMMAD NURSID, Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 7 (2020)
- EKO SETYOBUDI, IZANATUR ROHMAH, RIZKA FAUZIANA SYARIFAH, LISA RAMATIA, MURWANTOKO MURWANTOKO, DINI WAHYU KARTIKA SARI, Presence of Anisakis nematode larvae in Indian mackerel (Rastrelliger spp.) along the Indian Ocean southern coast of East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 20 No. 1 (2019)
- DINI SISWANI MULIA, ALIM ISNANSETYO, RARASTOETI PRATIWI, WIDYA ASMARA, Antibiotic resistance of Aeromonas spp. isolated from diseased walking catfish (Clarias sp.) , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 11 (2021)
- ALIM ISNANSETYO, DESY PUTRI HANDAYANI, INDAH ISTIQOMAH, ADITYA ARIF, TAKUSHI KANEKO, An antibacterial compound purified from a tropical coastal plant, Diospyros maritima , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 1 (2022)
- MURWANTOKO MURWANTOKO, SRI LAKSONO CONDRO NEGORO, ALIM ISNANSETYO, ZAFRAN ZAFRAN, Short Communication: Identification of marine leech and assessment of its prevalence and intensity on cultured hybrid groupers (Epinephelus sp.) , Biodiversitas Journal of Biological Diversity: Vol. 19 No. 5 (2018)
- KARUNIA ADETERA NUNGKI WIJAYANTI, INDAH ISTIQOMAH, MURWANTOKO, Bacterial abundance and community composition in green, brown and red water from intensive catfish (Clarias sp.) culture ponds in Yogyakarta, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 9 (2021)
- ALUSIA MELANITA RIA UTAMI, MURWANTOKO, INDAH ISTIQOMAH, TRIYANTO, EKO SETYOBUDI, Hysterothylacium amoyense (Nematoda: Raphidascarididae) infecting Trichiurus lepturus (Scombriformes: Trichiuridae) from Demak, Central Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 2 (2022)
- NISA QUROTA AYUN, LUTFI SEPTIANA DEWI, MURWANTOKO, EKO SETYOBUDI, The occurrence of Anisakis larvae on hairtail, Trichiurus lepturus caught from the Pangandaran Waters, West Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 3 (2021)