Tempeh flour as an excellent source of paraprobiotics
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Abstract. Jonesti WP, Prihatna C, Natadiputri GH, Suwanto A, Meryandini A. 2023. Tempeh flour as an excellent source of paraprobiotics. Biodiversitas 24: 1817-1823. Tempeh, a functional fermented food from Indonesia, has many benefits for human health. The presence of oligosaccharides, live and dead microbes in tempeh can function as prebiotics, probiotics, or paraprobiotics. This study aimed to determine the composition of live and dead lactic acid bacteria (LAB) and Enterobacteriaceae in tempeh flour using microbiological and molecular quantifications. Two samples of tempeh flour based on the drying process, i.e., sun-dried and oven-dried, were analyzed. Bacteria were quantified using standard plate count and qPCR, while bacterial cells damaged by the drying process were quantified using Propidium Monoazide-Quantitative Polymerase Chain Reaction (PMA-qPCR). Tempeh flours contained live bacteria as many as 4-5 log CFU/g and intact dead bacterial cells as many as 9 log CFU/g. The number of bacteria only differed slightly between the two drying processes. Based on qPCR analysis, the LAB number was higher than Enterobacteriaceae in tempeh flour. In addition, the bacterial population by qPCR was higher than by cultured analysis. These results indicated that sun-dried or oven-dried tempeh flour could be a functional food because it contained high amounts of live and dead bacteria as candidates for probiotics and paraprobiotics based on PMA-qPCR analysis.
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References
Astawan M, Wresdiyati T, Saragih AM. 2015. Evaluation of Protein Nutritional Quality of Tempeh and Boiled Soybean Flours by Rats. Indones J Food Qual 2(1):11–17.
Broeders S, Huber I, Grohmann L, Berben G, Taverniers I, Mazzara M, Roosens N, Morisset D. 2014. Guidelines for Validation of Qualitative Real-Time PCR Methods. Trends Food Sci Technol 37(2):115–26. DOI: 10.1016/j.tifs.2014.03.008.
Buckley M, Lacey S, Doolan A, Goodbody E, Seamans K. 2018. The effect of Lactobacillus reuteri supplementation in Helicobacter pylori infection: A placebo-controlled, single-blind study. BMC Nutr 4(1):1–8. DOI: 10.1186/s40795-018-0257-4.
Efriwati, Suwanto A, Rahayu G, Nuraida L. 2013. Population Dynamics of Yeasts and Lactic Acid Bacteria (LAB) During Tempeh Production. HAYATI J Biosci 20(2):57–64. DOI: 10.4308/hjb.20.2.57.
Erdiansyah M, Meryandini A, Wijaya M, Suwanto A. 2021. Microbiological quality of tempeh with different wraps: banana leaf versus plastic. J Food Sci Technol 59(1):300–307. DOI: 10.1007/s13197-021-05014-7.
Erkus O, de Jager VCL, Geene RTCM, van Alen-Boerrigter I, Hazelwood L, van Hijum SAFT, Kleerebezem M, Smid EJ. 2016. Use of propidium monoazide for selective profiling of viable microbial cells during Gouda cheese ripening. Int J Food Microbiol 228 1–9. DOI: 10.1016/j.ijfoodmicro.2016.03.027.
Everis L. 2001. Injured bacteria in foods. Nutr Food Sci 31(2):84–88. DOI: 10.1108/00346650110367008.
Hassanein TR, Prabawati EK, Gunawan-Puteri DMTP. 2015. Analysis of Chemical and Microbial Changes During Storage of Overripe Tempeh Powder as Seasoning Material. Int J Sci Eng 8(2):131–134. DOI: 10.12777/IJSE.8.2.131-134.
Huang L, Huang Z, Zhang Y, Zhou S, Hu W, Dong M. 2019. Impact of tempeh flour on the rheology of wheat flour dough and bread staling. LWT-Food Sci Technol 111:694–702. DOI: 10.1016/J.LWT.2019.04.004.
Ilha E C, Scariot MC, Treml D, Pereira TP, Sant?Anna ES, Prudêncio ES, Arisi ACM. 2016. Comparison of real-time PCR assay and plate count for Lactobacillus paracasei enumeration in yoghurt. Ann Microbiol 66(2):597–606. DOI: 10.1007/s13213-015-1137-7.
Ilham HM, Wijaya M, Suwanto A, Rusmana I. 2021. Dominant Enterobacteriaceae in tempeh were primarily originated from soybean. Food Sci Biotechnol 30(6):861–868. DOI: 10.1007/s10068-021-00915-x.
Li B, Chen JQ. 2012. Real-time PCR methodology for selective detection of viable Escherichia coli O157: H7CELLS by targeting Z3276 as a genetic marker. Appl and Environ Microbiol 78(15):5297–5304. DOI: 10.1128/AEM.00794-12.
Murata M, Kondo J, Iwabuchi N, Takahashi S, Yamauchi K, Abe F, Miura K. 2018. Effects of paraprobiotic Lactobacillus paracasei MCC1849 supplementation on symptoms of the common cold and mood states in healthy adults. Benef Microbes 9(6):855–864. DOI: 10.3920/BM2017.0197.
Nocker A, Sossa-Fernandez P, Burr MD, Camper AK. 2007. Use of propidium monoazide for live/dead distinction in microbial ecology. Appl Environ Microbiol 73(16):5111-5117. DOI: 10.1128/AEM.02987-06.
Nout MJR, Kiers JL. 2005. Tempeh fermentation, innovation and functionality: Update into the third millenium. J Appl Microbiol 98(4):789–805. DOI: 10.1111/j.1365-2672.2004.02471-x.
Pontonio E, Cagno RDi, Mahony J, Lanera A, Angelis MDe, Van Sinderen D, Gobbetti M. 2017. Sourdough authentication: Quantitative PCR to detect the lactic acid bacterial microbiota in breads. Sci Rep 7(1):1–12. DOI: 10.1038/s41598-017-00549-2.
Prihatna C, Pramudito TE, Arifin AR, Nguyen TKN, Purnamasari MI, Suwanto A. 2022. Antifungal Peptides from a Burkholderia Strain Suppress Basal Stem Rot Disease of Oil Palm. Phytopathology 112(2):238-248. DOI: 10.1094/PHYTO-11-20-0529-R.
Rittershaus ESC, Baek SH, Sassetti CM. 2013. The normalcy of dormancy: Common themes in microbial quiescence. Cell Host Microbe. 13(6):643–651. DOI: 10.1016/j.chom.2013.05.012.
Romulo A, Surya R. 2021. Tempe: A traditional fermented food of Indonesia and its health benefits. Int J Gastron Food Sci 26:100413. DOI: 10.1016/j.ijgfs.2021.100413.
Sawada D, Sugawara T, Ishida Y, Aihara K, Aoki Y, Takehara I, Takano K, Fujiwara S. 2016. Effect of continuous ingestion of a beverage prepared with Lactobacillus gasseri CP2305 inactivated by heat treatment on the regulation of intestinal function. Food Res Int 79:33–39. DOI: 10.1016/j.foodres.2015.11.032.
Scariot MC, Venturelli GL, Prudêncio ES, Arisi ACM. 2018. Quantification of Lactobacillus paracasei viable cells in probiotic yoghurt by propidium monoazide combined with quantitative PCR. Int J Food Microbiol 264:1–7. DOI: 10.1016/j.ijfoodmicro.2017.10.021.
Schwendimann L, Kauf P, Fieseler L, Gantenbein-Demarchi C, Miescher Schwenninger S. 2015. Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation. J Microbiol Methods 115:94–99. DOI: 10.1016/j.mimet.2015.05.022.
Siciliano RA, Reale A, Mazzeo MF, Morandi S, Silvetti T, Brasca, M (2021) Paraprobiotics: A new perspective for functional foods and nutraceuticals. Nutrients 13(4):1–19. DOI: 10.3390/nu13041225.
Soejima T, Minami J, Iwatsuki K. 2012. Rapid propidium monoazide PCR assay for the exclusive detection of viable Enterobacteriaceae cells in pasteurized milk. J Dairy Sci 95(7):3634–3642. DOI: 10.3168/jds.2012-5360.
Soenarto Y, Sudigbia I, Herman H, Karmini M, Karyadi D. 2001. Antidiarrheal characteristics of tempe produced traditionally and industrially in children aged 6-24 months with acute diarrhea. Paediatr Indones 41(3-4):88-95.
Stephanie, Kartawidjajaputra F, Silo W, Yogiara Y, Suwanto A. 2019. Tempeh consumption enhanced beneficial bacteria in the human gut. Food Res 3(1):57–63. DOI: 10.26656/fr.2017.3(1).230.
Stephanie S, Ratih NK, Soka S, Suwanto A. 2017. Effect of tempeh supplementation on the profiles of human intestinal immune system and gut microbiota. Microbiol Indones 11(1):2. DOI: 10.5454/mi.11.1.2.
Takahashi H, Saito R, Miya S, Tanaka Y, Miyamura N, Kuda T, Kimura B. 2017. Development of quantitative real-time PCR for detection and enumeration of Enterobacteriaceae. Int J Food Microbiol 246:92–97. DOI: 10.1016/j.ijfoodmicro.2016.12.015.
Tampubolon NL, Karo-karo T, Ridwansyah. 2014. Formulations of Instant Baby Porridge with Tempeh Flour and Pumpkin Flour Substitution as an Alternative Complementary Breast. Jurnal Rekayasa Pangan dan Pertanian 2(2):78–83.
Yulandi A, Suwanto A, Waturangi DE, Wahyudi AT. 2020. Shotgun metagenomic analysis reveals new insights into bacterial community profiles in tempeh. BMC Res Notes 13(1):1–7. DOI: 10.1186/s13104-020-05406-6.
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