Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia
##plugins.themes.bootstrap3.article.sidebar##
Issue
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Isnansetyo A, Istiqomah I, Anshary H, Sriwulan S, Yudiati E, Subagiyo S, Arif A, Kartikasari DW. 2022. Identification and antibiotic-resistant properties of Vibrio owensii and V. alginolyticus isolated from the Spermonde Islands, Indonesia. Biodiversitas 23: 5995-6005. The Spermonde Islands are located in Makassar Strait, southern Sulawesi, Indonesia, and have attracted the attention of researchers for decades because of their mega biodiversity and abundance of bioresources. However, no study has evaluated the potential mariculture diseases in this area. The present study assessed the potential bacterial fish diseases based on the current status of mariculture in the Spermonde Islands. The samples were collected from three marine aquaculture sites at Barrang Caddi and the Samaloa Islands. Vibrio was isolated on Thiosulfate-citrate-bile-sucrose (TCBS) agar medium. The most common bacterial diseases were observed by bacterial isolation, morphological and biochemical observations, and molecular identification based on 16S rDNA sequence. The microbial, physical, and chemical water qualities at Barrang Caddi and Samaloa Island were suitable for marine aquaculture. Although cultured species in this area did not exhibit gross disease signs, potential opportunistic pathogens of Vibrio were found. The 16S rDNA sequences analysis indicated that the bacterial isolates closed to Vibrio owensii, V. alginolyticus, and V. neocaledonius. Multi-drug resistant V. owensii was identified as a potential pathogen in marine aquaculture. This is the first report on V. owensii isolated from Indonesian marine waters, particularly from the Spermonde Islands. V. alginolyticus was another potential opportunistic pathogen in this area. These results gave the alarm to develop countermeasure methods for potential diseases to minimize the possible outbreak of vibriosis.
##plugins.themes.bootstrap3.article.details##
References
Amin AK, Feng G, Al-Saari N, Meirelles PM, Yamazaki Y, Mino S, Thompson FL, Sawabe T. 2016. The first temporal and spatial assessment of Vibrio diversity of the surrounding seawater of coral reefs in Ishigaki, Japan. Front. Microbiol. 7: 1185. https://doi.org/10.3389/fmicb.2016.01185
Chalkiadaki E, Dufourcq R, Schmitt S, Brandily C, Kervarec N, Coatanea D, Amir H, Loubersac L, Chanteau S, Guezennec J, Dupont-Rouzeyrol M, Simon-Colin C. 2015. Partial characterization of an exopolysaccharide secreted by a marine bacterium, Vibrio neocaledonicus sp. nov., from New Caledonia. J. Appl. Microbiol. 114:1702-1712. DOI: 10.1111/jam.12184
Chau KM, Van TT, Quyen DV, Le HD, Phan TH, Ngo ND, Vo TD, Dinh TT, Le HT, Khanh HH. 2021. Molecular identification and characterization of probiotic Bacillus species with the ability to control Vibrio spp. in wild fish intestines and sponges from the Vietnam Sea. Microorganisms 9: 1927. https://doi.org/10.3390/microorganisms9091927
Chen Y, Wu F, Wang Z, Tang J, Cai S, Jian J. 2020. Construction and evaluation of Vibrio alginolyticus ?clpP mutant, as a safe live attenuated vibriosis vaccine. Fish Shellfish Immunol. 98: 917-922.
Colquhoun DJ, Lillehaug A. 2014. Vaccination against vibriosis. Fish vaccination 12: 172-184. https://doi.org/10.1002/9781118806913.ch15
Dai L, Xiong Z, Hou D, Wang Y, Li T, Long X, Chen H, Sun C. 2022. Pathogenicity and transcriptome analysis of a strain of Vibrio owensii in Fenneropenaeus merguiensis. Fish Shellfish Immunol. 132: 194-205. DOI: https://doi.org/10.1016/j.fsi.2022.09.008
El-Sayed M, Algammal A, Abouel-Atta M, Mabrok M, Emam A. 2019. Pathogenicity, genetic typing, and antibiotic sensitivity of Vibrio alginolyticus isolated from Oreochromis niloticus and Tilapia zillii. Rev. Med. Vet. 170: 80-86. DOI: 10.3923/pjbs.2020.1591.1600.
Emam AM, Hashem M, Gadallah AO, Haridy M. 2019. An outbreak of Vibrio alginolyticus infection in aquarium-maintained dark-spotted (Himantura uarnak) and Tahitian (H. fai) stingrays. The Egyptian Journal of Aquatic Research 45: 153-158. doi: 10.1016/j.ejar.2019.05.003.
Food and Agriculture Organization (FAO). 2018. The state of world fisheries and aquaculture 2018 – Meeting the sustainable development goals. Rome, Italy. 227 p.
Food and Agriculture Organization (FAO). 2020. FAO Yearbook. Fishery and Aquaculture Statistics 2018. Rome. Italy. 110 p. doi: 10.4060/cb1213t.
Galindo-Villegas J, Mulero I, García-Alcazar A, Muñoz I, Peñalver-Mellado M, Streitenberger S, Scapigliati G, Meseguer J, Mulero V. 2013. Recombinant TNF? as oral vaccine adjuvant protects European sea bass against vibriosis: insights into the role of the CCL25/CCR9 axis. Fish Shellfish Immunol 35: 1260-1271. https://doi.org/10.1016/j.fsi.2013.07.046
Cano-Gómez A, Goulden EF, Owens L, Høj L. 2010. Vibrio owensii sp.nov., isolated from cultured crustaceans in Australia. FEMS Microbiol Lett 302: 175-181. https://doi.org/10.1111/j.1574-6968.2009.01850.x
Gómez-Betancur I, Zhao J, Tan L, Chen C, Yu G, Rey-Suárez P, Preciado L. 2019. Bioactive compounds isolated from marine bacterium Vibrio neocaledonicus and their enzyme inhibitory activities. Marine Drugs 17: 401. https://doi.org/10.3390/md17070401
Goulden EF, Hall MR, Bourne DG, Pereg LL, Høj L. 2012. Pathogenicity and infection cycle of Vibrio owensii in larviculture of the ornate spiny lobster (Panulirus ornatus). Appl. Environ. Microbiol. 78: 2841-2849. https://doi.org/10.1128/AEM.07274-11
Hannan MA, Rahman MM, Mondal MN, Chandra DS, Chowdhury G, Islam MT. 2019. Molecular identification of Vibrio alginolyticus causing vibriosis in shrimp and its herbal remedy. Pol. J. Microbiol. 68: 429-438. https://doi.org/10.33073/pjm-2019-042
Hernández-Robles MF, Álvarez-Contreras AK, Juárez-García P, Natividad-Bonifacio I, Curiel-Quesada E, Vázquez-Salinas C, Quiñones-Ramírez EI. 2016. Virulence factors and antimicrobial resistance in environmental strains of Vibrio alginolyticus. Int. Microbiol. 19: 191-198. doi:10.2436/20.1501.01.277.
Ina?Salwany MY, Al?saari N, Mohamad A, Mursidi FA, Mohd?Aris A, Amal MN, Kasai H, Mino S, Sawabe T, Zamri?Saad M. 2019. Vibriosis in fish: A review on disease development and prevention. J. Anim. Health 31: 3-22. https://doi.org/10.1002/aah.10045
Isnansetyo A, Getsu S, Seguchi M, Koriyama M. 2014. Independent effects of temperature, salinity, ammonium concentration and ph on nitrification rate of the ariake seawater above mud sediment. HAYATI Journal of Biosciences 21: 21-30. doi: 10.4308/hjb.21.1.21.
Isnansetyo A, Istiqomah I, Sinansari S, Hernawan RK, Widada J. 2009. A potential bacterial biocontrol agent, strain S2V2 against pathogenic marine Vibrio in aquaculture. World J. Microbiol. Biotechnol. 25: 1103-1113. DOI 10.1007/s11274-009-9992-7
Istiqomah I, Sukardi, Murwantoko, Isnansetyo A. 2020. Review vibriosis management in Indonesian marine fish farming. In E3S Web of Conferences, EDP Sciences 147: 01001.
Ji Q, Wang S, Ma J, Liu Q. 2020. A review: progress in the development of fish Vibrio spp. vaccines. Immunol. Lett. 226: 46-54. https://doi.org/10.1016/j.imlet.2020.07.002
Kasnir M. 2018. Analisis aspek ekologi penatakelolaan minawisata bahari di Kepulauan Spermonde Kabupaten Pangkep, Sulawesi Selatan. Ilmu Kelautan 16: 61-69. https://doi.org/10.14710/ik.ijms.16.2.61-69
Kegler HF, Lukman M, Teichberg M, Plass-Johnson J, Hassenrück C, Wild C, Gärdes A. 2017. Bacterial community composition and potential driving factors in different reef habitats of the Spermonde Archipelago, Indonesia. Front. Microbiol. 8: 662. https://doi.org/10.3389/fmicb.2017.00662
Teichberg M, Wild C, Bednarz VN, Kegler HF, Lukman M, Gärdes AA, Heiden JP, Weiand L, Abu N, Nasir A, Miñarro S. 2017. Reef island evolution and dynamics: insights from the indian and pacific oceans and perspectives for the spermonde archipelago. Front. Mar. Sci. 4: 145. https://doi.org/10.3389/fmars.2018.00033
Kegler HF, Lukman M, Teichberg M, Plass-Johnson J, Hassenrück C, Wild C, Gärdes A. 2017. Bacterial community composition and potential driving factors in different reef habitats of the Spermonde Oceans and perspectives for the Spermonde Archipelago. Front. Microbiol. 8: 662. https://doi.org/10.3389/fmicb.2017.00662
Kim SG, Jun JW, Giri SS, Yun S, Kim HJ, Kim SW, Kang JW, Han SJ, Jeong D, Park SC. 2019. Isolation and characterisation of pVa-21, a giant bacteriophage with anti-biofilm potential against Vibrio alginolyticus. Scientific reports 9: 1-10. https://doi.org/10.1038/s41598-019-42681-1
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol. Biol. Evol. 35: 1547-1549. doi: 10.1093/molbev/msy096
Letchumanan V, Chan KG, Pusparajah P, Saokaew S, Duangjai A, Goh BH, Ab Mutalib NS, Lee LH. 2016. Insights into bacteriophage application in controlling Vibrio species. Front. Microbiol. 7: 1114. https://doi.org/10.3389/fmicb.2016.01114
Li H, Zhang X, Long H, Hu C, Zhou Y, Wang S, Ke S, Xie Z. 2019. Vibrio alginolyticus 16S-23S intergenic spacer region analysis, and PCR assay for identification of coral pathogenic strain XSBZ03. Microb. Pathog. 126: 165-171. https://doi.org/10.3354/dao03233
Lin LC, Lin GH, Tseng YH, Yu MS. 2015. Draft genome sequence of Vibrio owensii GRA50-12, isolated from green algae in the intertidal zone of eastern Taiwan. Genome Announcement 3: 01438-14. doi: 10.1128/genomeA.01438-14.
Liu F, Li S, Yu Y, Yuan J, Yu K, Li F. 2021. Pathogenicity of a Vibrio owensii strain isolated from Fenneropenaeus chinensis carrying pirAB genes and causing AHPND. Aquaculture 530: 735-747. https://doi.org/10.1016/j.aquaculture.2020.735747
Liu L, Xiao J, Zhang M, Zhu W, Xia X, Dai X, Pana Y, Yana S, Wanga Y. 2018. A Vibrio owensii strain as the causative agent of AHPND in cultured shrimp, Litopenaeus vannamei. J. Invertebr. Pathol. 153: 156-164. doi: 10.1016/j.jip.2018.02.005.
Liu PC, Lin JY, Hsiao PT, Lee KK. 2019. Isolation and characterization of pathogenic Vibrio alginolyticus from diseased cobia Rachycentron canadum. Pol. J. Microbiol. 68: 429-438. https://doi.org/10.1002/jobm.200310316
Mechri B, Monastiri A, Medhioub A, Medhioub MN, Aouni M. 2018. Molecular characterization and phylogenetic analysis of highly pathogenic Vibrio alginolyticus strains isolated during mortality outbreaks in cultured Ruditapes decussatus juvenile. Dis. Aqua.t Organ. 129: 71-83. https://doi.org/10.1016/j.micpath.2017.09.020
Mohamad A, Zamri-Saad M, Amal MN, Al-Saari N, Monir MS, Chin YK, Md Yasin IS. 2021. Vaccine efficacy of a newly developed feed-based whole-cell polyvalent vaccine against vibriosis, streptococcosis and motile aeromonad septicemia in Asian Seabass, Lates calcarifer. Vaccines 9: 368. https://doi.org/10.3390/vaccines9040368
National Committee for Clinical Laboratory Standards (NCCLS). 1994. Performance standards for antimicrobial disk and dilution susceptibility tests for bacterial isolated from animals; proposed standard. NCCLS Document M31-P 14:1-29.
Nishiki I, Minami T, Murakami A, Hoai TD, Fujiwara A. 2018. Multilocus sequence analysis of Vibrionaceae isolated from farmed amberjack and the development of a multiplex PCR assay for the detection of pathogenic species. J. Fish Dis. 41: 1295-1301. https://doi.org/10.1111/jfd.12823
Rameshkumar P, Nazar AK, Pradeep MA, Kalidas C, Jayakumar R, Tamilmani G, Sakthivel M, Samal AK, Sirajudeen S, Venkatesan V, Nazeera BM. 2017. Isolation and characterization of pathogenic Vibrio alginolyticus from sea cage cultured cobia (Rachycentron canadum (Linnaeus 1766)) in India. Microb. Pathog. 111:487-496. https://doi.org/10.1111/lam.12800
Sadok K, Mejdi S, Nourhen S, Amina B. 2019. Phenotypic characterization and RAPD fingerprinting of Vibrio parahaemolyticus and Vibrio alginolyticus isolated during Tunisian fish farm outbreaks. J. Aquat. Anim. Health 58: 17-26. https://doi.org/10.1007/s12223-012-0174-x
Saitou N, Nei M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Smibert RM, Krieg NR. 1994. Phenotypic characterization. In: P Gerhardt, RGE Murray, WA Wood, NR Krieg (eds) Methods for general, molecular bacteriology. American Society for Microbiology, Washington 607-654. doi: 10.1002/food.19960400226
Tamura K, Nei M, Kumar S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101: 11030-11035.
Thillaichidambaram M, Narayanan K, Selvaraj S, Sundararaju S, Muthiah RC, Figge MJ. 2022. Isolation and characterization of Vibrio owensii from Palk Bay and its infection study against post larvae of Litopenaeus vannamei. Microbial Pathogenesis. 172: 105751. DOI: https://doi.org/10.1016/j.micpath.2022.105751
Urbanczyk Y, Ogura Y, Hayashi T, Urbanczyk H. 2015. Description of a novel marine bacterium, Vibrio hyugaensis sp. nov.,based on genomic and phenotypic characterization. Syst. Appl. Microbiol. 38: 300-304. https://doi.org/10.1016/j.syapm.2015.04.001
Ushijima B, Smith A, Aeby GS, Callahan SM. 2012. Vibrio owensii induces the tissue loss disease montipora white syndrome in the Hawaiian reef coral Montipora capitata. PLoS ONE 7: e46717. https://doi.org/10.1371/journal.pone.0046717
Yasir M, Ullah R, Bibi F, Khan SB, Al-Sofyani AA, Stingl U, Azhar EI. 2020. Draft genome sequence of a multidrug-resistant emerging pathogenic isolate of Vibrio alginolyticus from the Red Sea. New Microbe and New Infect 38: 100804. https://doi.org/10.1016/j.nmni.2020.100804
Ye L, Li R, Lin D, Zhou Y, Fu A, Ding Q, Chan EW, Yao W, Chen S. 2016. Characterization of an IncA/C Multidrug Resistance Plasmid in Vibrio alginolyticus. Antimicrob. Agents Chemother. 60: 3232–3235. DOI: https://doi.org/10.1128/AAC.00300-16
Yoshizawa S, Tsuruya Y, Fukui Y, Sawabe T, Yokota A, Kogure K, Higgins M, Carson J, Thompson FL. 2012. Vibrio jasicida sp. nov., a member of the Harveyi clade, isolated from marine animals (packhorse lobster, abalone and Atlantic salmon). Int. J. Syst. Evol. Microbiol. 62: 1864-1870. https://doi.org/10.1099/ijs.0.025916-0
Yu YP, Gong T, Jost G, Liu WH, Ye DZ, Luo ZH. 2013. Isolation and characterization of five lytic bacteriophages infecting a Vibrio strain closely related to Vibrio owensii. FEMS Microbiol. Lett. 348: 112-119. https://doi.org/10.1111/1574-6968.12277
Yusuf S, Jompa J. 2012. Managing bleached coral reefs first quantitative assessment of coral bleaching on Indonesian reefs. Proceedings of the 12th international coral reef symposium, Cairns, Australia 9-13 July 2012, 17D.
Most read articles by the same author(s)
- KARUNIA ADETERA NUNGKI WIJAYANTI, INDAH ISTIQOMAH, MURWANTOKO, Bacterial abundance and community composition in green, brown and red water from intensive catfish (Clarias sp.) culture ponds in Yogyakarta, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 9 (2021)
- ALUSIA MELANITA RIA UTAMI, MURWANTOKO, INDAH ISTIQOMAH, TRIYANTO, EKO SETYOBUDI, Hysterothylacium amoyense (Nematoda: Raphidascarididae) infecting Trichiurus lepturus (Scombriformes: Trichiuridae) from Demak, Central Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 2 (2022)
- AMRIANA, DWI KESUMA SARI, SRIWULAN, HILAL ANSHARY, Evaluation of Argulus indicus on striped snakehead (Channa striata) in Towuti Lake, South Sulawesi, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 3 (2022)
- DINI SISWANI MULIA, RAIH RAICHA, CITRA PUSPANING LUNGGANI, SISKA NUR ERINA, JULI ROCHMIJATI WULIANDARI, CAHYONO PURBOMARTONO, ALIM ISNANSETYO, Antibacterial activity of ethanol extract of banana, cassava, and pineapple peels against a fish pathogen Aeromonas hydrophila , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 1 (2023)
- DINI SISWANI MULIA, SUCI DWI RAHAYU, AMAN SUYADI, IKHSAN MUJAHID, ALIM ISNANSETYO, Antibacterial activity of mangrove plant extract of Rhizophora apiculata in inhibiting the growth of various strains of Aeromonas hydrophila , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 9 (2023)
- INDAH ISTIQOMAH, ALIM ISNANSETYO, MURWANTOKO, DESY PUTRI HANDAYANI, YANI NUR’AINI LESTARI, ARIEF TASLIHAN, I GUSTI NGURAH PERMANA, ENDANG WIJAYANTI, Antibiotic resistance of emerging pathogenic bacteria of hybrid grouper farming in Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 4 (2023)
- MARYROSE EASTER TAPILATU, DIAH PERMATA WIJAYANTI, SUBAGIYO, ANDRIANUS SEMBIRING, NI LUH ASTRIA YUSMALINDA, ENEX YUNIARTI NINGSIH, MUHAMMAD DANIE AL MALIK, NI PUTU DIAN PERTIWI, Genetic diversity of wedgefishes and guitarfishes at landing sites in east Indonesia using Cytochrome Subunit I (COI) , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 6 (2023)
- ERVIA YUDIATI, TRISTIANA YUNIARTI, AGUS TRIANTO, ZAENAL ARIFIN, LISA RULIATY, M. SOLEH, AGUSTIEN NARYANINGSIH, SURYATI, The reproduction performance of catching Planiliza subviridis (Teleostei: Mugilidae) in different moon periods from Jepara Coast, Central Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 4 (2024)
- IKA, HILAL ANSHARY, SRIWULAN, Infestation and histopathology of Clinostomum sp. (Digenea: Clinostomidae) in snakeskin fish (Trichopodus pectoralis Regan, 1910) in Tempe Lake, South Sulawesi, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 25 No. 9 (2024)