Diversity of Arbuscular Mycorrhizal Fungi of Kalappia celebica: An endemic and endangered plant species in Sulawesi, Indonesia
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Abstract. Husna, Tuheteru FD, Albasri, Arif A, Basrudin, Nurdin WR, Arman E, Agustin DI, Saribadu J, Rahmat, Dermawansyah A, Daliana, Lody LP, Deri AS, Safitri I. 2022. Diversity of Arbuscular Mycorrhizal Fungi of Kalappia celebica.): An endemic and endangered plant species in Sulawesi, Indonesia. Biodiversitas 23: 5290-5297. Arbuscular Mycorrhizal Fungi (AMF) are a group of fungi belonging to Phylum Glomeromycota. AMF has a symbiotic relationship with higher plants, including endangered species in various climatic regions and land types. Research on AMF diversity related to endemic and endangered tree species in the tropics is still limited. This study aimed to examine the diversity of AMF in the natural habitat of Kalappia celebica Kosterm. (kalapi) in Southeast Sulawesi Province, Indonesia. Samples of soil and roots of K. celebica were collected from six natural habitat locations, i.e., Konawe District (Anggatoa and Abuki Villages), East Kolaka District (Lalingato and Anggaloosi Villages) and Kolaka District (Lalonaha and Lapao Pao Villages). The AMF spores were isolated by using the pouring method, followed by wet filtration. AMF identification was carried out by observing the morphology of AMF spores. A total of eight AMF species belonging to three genera Viz. Glomus, Funneliformis and Rhizophagus were isolated. Among the species identified, five species belong to genus Glomus, two species belong to the genus Funneliformis and one species belongs to the genus Rhizophagus. Glomus sp.1 and 2 and F. mosseae had the highest spore density, relative density and frequency, and importance index values. The AMF highest spore density was obtained in Anggaloosi Village of East Kolaka District. Soil properties such as organic C, total N and clay were positively correlated with spore density and negatively correlated with AMF colonization. The Simpson’s Index ranged from 0.42 ± 0.117 to 0.86 ± 0.068. The range of Shannon-Wiener Index was 0.12 ± 0.208 to 0.99 ± 0.198, while the Evenness Index ranged from 0.17 ± 0.189 to 0.75 ± 0.106. These findings indicated that K. celebica species are rich in AMF diversity. These symbiont fungi are key components of the ecosystem. Further research is needed to develop their use as promoters of plant establishment in conservation and restoration of those sites.
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References
Abdedaiem R, Rejili M, Mahdhi M, Lajudie dP, Mars M. 2020. Phylogeny and distribution of arbuscular mycorrhizal fungi associated with Vachellia tortilis ssp. raddiana in relation to soil properties under arid ecosystems of Tunisia. Mycol Progress 19, 495–504. doi: 10.1007/s11557-020-01572-w
Arif A, Husna, FD Tuheteru, A Adiansyah. 2021. The conservation of endemic and endangered tree species Kalapia celebica Kosterm with Mycorrhizal bio-fertilizer technology in post-gold mining areas. IOP Conf. Series: Earth and Environmental Science 800 (2021) 012039
Arif A, Tuheteru FD, Husna, Kandari AM, Mekuo IS, Masnun. 2016. Status and culture of arbuscular mycorrhizal fungi isolated from rhizosphere of endemic and endangered species of kalapi (Kalappia celebica Kosterm.). Eur J Sustain Dev 5(4): 395-402. doi: 10.14207/ejsd.2016.v5n4p395
Arif A, Husna, Tuheteru FD, Rosnawati. 2022. Shoots cuttings propagation of endangered and endemic tree species of Kalappia celebica Kosterm using the application of Rootone-f. Agriculture and Forestry, 68 (2): 121-131. doi:10.17707/AgricultForest.68.2.09
Barroetavena C, Gisler S, Luoma DL, Meinke RJ. 1986. Mycorrhizal status of the endangered species Astragalus applegatei Peck as determined from a soil bioassay. Mycorrhiza 8, 117–119. doi: 10.1007/s005720050222
Becerra AG, Arrigo NM, Bartoloni N, Dominguez LS, Cofre MN. 2007. Arbuscular mycorrhizal colonization of Alnus acuminate Kunth in northwestern Argentina in relation to season and soil parameters. Ci Suelo 25(1):7–13
Binet MN, van Tuinen D, Deprêtre N. et al. 2011. Arbuscular mycorrhizal fungi associated with Artemisia umbelliformis Lam, an endangered aromatic species in Southern French Alps, influence plant P and essential oil contents. Mycorrhiza 21, 523–535. doi: 10.1007/s00572-010-0354-y
Bothe H, Turnau K, Regvar M. 2010. The potential role of arbuscular mycorrhizal fungi in protecting endangered plants and habitats [review]. Mycorrhiza. 20:445-457
Brundrett M, N Bougher, B Deu, TGrove, Majalaczuk. 1996. Working with Mycorrhizas in Forestry and Agriculture. Canberra (Australia) : Australian Centre for International Agriculture Research.
Brundrett MC, Tedersoo L. 2018. Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytol 220:1108–1115. doi: 10.1111/nph.14976
Ceola G, Goss-Souza D, Alves J. et al. 2021. Biogeographic Patterns of Arbuscular Mycorrhizal Fungal Communities Along a Land-Use Intensification Gradient in the Subtropical Atlantic Forest Biome. Microb Ecol 82, 942–960. doi: 10.1007/s00248-021-01721-y
Chaudhary VB, Cuenca G,?Johnson NC. 2018. Tropical-temperate comparison of landscape-scale arbuscular mycorrhizal fungal species distributions. Diversity and Distributions, 24:116–128. doi: 10.1111/ddi.12664
Chaudhary VB, Lau MK, Johnson NC. 2008. Macroecology of Microbes – Biogeography of the Glomeromycota. In: Varma, A. (eds) Mycorrhiza. Springer, Berlin, Heidelberg. doi: 10.1007/978-3-540-78826-3_26
Crossay T, Cilia A, Cavaloc Y. et al. 2018. Four new species of arbuscular mycorrhizal fungi (Glomeromycota) associated with endemic plants from ultramafic soils of New Caledonia. Mycol Progress 17, 729–744. doi: 10.1007/s11557-018-1386-5
Danesh YR, Kariman K, Keskin N, Najafi S. 2022. Characterization of arbuscular mycorrhizal fungal communities associated with vineyards in northwestern Iran. Turk J Agric For, 46: 271-279. doi:10.55730/1300-011X.3001
Diagne N, M Ngom, PI Djighaly, D Fall, V Hocher, S Svistoonof. 2020. Roles of Arbuscular Mycorrhizal Fungi on Plant Growth and Performance: Importance in Biotic and Abiotic Stressed Regulation. Diversity, 12, 370
Evelin H, Sharma E, Kapoor R. 2019. Arbuscular mycorrhizal fungi: potential role in conservation of endangered plants. Plant Reproductive Biology: 314–326
Furrazola E, Sa?nchez-Rendo?n JA, Guadarrama P, Pernu?s M, Torres-Arias Y. 2020. Mycorrhizal status of Coccothrinax crinita (Arecaceae), an endangered endemic species from western Cuba. Revista Mexicana de Biodiversidad 91: e913048
Gardeman JW, Trappe JM. 1974. The Endogonaceae in the Pacific Northwest. Mycologia Memoir No 5. 76 pp.
Husna, FD Tuheteru, Arif A. 2018. Arbuscular Mycorrhizal Fungi Symbiosis and Conservation of Endangered Tropical Legume Trees. In : B. Giri et al. (eds.), Root Biology, Soil Biology 52. Springer. Germany
Husna, FD Tuheteru, A Arif. 2021b. Arbuscular mycorrhizal fungi to enhance the growth of tropical endangered species Pterocarpus indicus and Pericopsis mooniana in post gold mine field in Southeast Sulawesi, Indonesia. Biodiversitas, 22(9): 3844-3853
Husna, FD Tuheteru, A. Arif, Solomon. 2019a. Improvement of early growth of endemic sulawesi trees species kalappia celebica by arbuscular mycorrhizal fungi in gold mining tailings. IOP Conf. Ser.: Earth Environ. Sci. 394 012069
Husna, Mansur I, Budi SWR, Tuheteru FD, Arif A, Tuheteru ED, Albasri. 2019. Effects of arbuscular mycorrhizal fungi and organic material on growth and nutrient uptake by Pericopsis mooniana in coal mine. Asian J. Plant Sci., 18: 101-109
Husna, SW Budi R, Mansur I, C Kusmana. 2015. Diversity of arbuscular mycorrhizal fungi in the growth habitat of Kayu Kuku (Pericopsis mooniana Thw.) In South east Sulawesi). Pakistan Journal of Biological Science18 (1): 1-10
Husna, SW Budi R, Mansur I, C Kusmana. 2016. Growth and nutrient status of Kayu Kuku (Pericopsis mooniana Thw.) with micorrhiza in soil media of nickel post mining. Pakistan Journal of Biological Science 19 : 158-170
Husna, Tuheteru FD, Arif A. 2021a. The potential of arbuscular mycorrhizal fungi to conserve Kalappia celebica, an endangered endemic legume on gold mine tailings in Sulawesi, Indonesia. J For Res 32: 675-682
Jishtu V, Kapoor R, Singh J, TN Lakhanpal. 2019. Arbuscular Mycorrhizal (AM) diversity in some threatened North West Himalayan flora of Kinnaur. KAVAKA 52: 42-51
Kivlin SN, Christine VH, KK Treseder. 2011. Global diversity and distribution of arbuscular mycorrhizal fungi. Soil Biology & Biochemistry 43:2294-2303
Luo Y, Wang Z, He Y, Li G, Xinhua Lv, Zhuang L. 2020. High-throughput sequencing analysis of the rhizosphere arbuscular mycorrhizal fungi (AMF) community composition associated with Ferula sinkiangensis. BMC Microbiology, 20:335. doi: 10.1186/s12866-020-02024-x
Melo CD, Walker C, Krüger C. et al. 2019. Environmental factors driving arbuscular mycorrhizal fungal communities associated with endemic woody plant Picconia azorica on native forest of Azores. Ann Microbiol 69, 1309–1327. doi: 10.1007/s13213-019-01535-x
Mirzaei J, Moradi M. 2017. Biodiversity of arbuscular mycorrhizal fungi in Amygdalus scoparia Spach plantations and a natural stand. J. For. Res. 28, 1209–1217. doi: 10.1007/s11676-017-0392-9
Mitra D, R. Djebaili, M. Pellegrini, B. Mahakur, A. Sarker, P.Chaudhary, B. Khoshru, MD Gallo, M. Kitouni, DP. Barik, P. Panneerselvam, PK. Das Mohapatra. 2021. Arbuscular mycorrhizal symbiosis: plant growth improvement and induction of resistance under stressful conditions, Journal of Plant Nutrition, doi: 10.1080/01904167.2021.1881552
Ogoma BO, Omondi SF, Ngaira J, Kimani, JW. 2021. Molecular diversity of arbuscular mycorrhizal fungi (AMF) associated with Carissa edulis, an Endangered Plant Species along Lake Victoria Basin of Kenya. International Journal of Forestry Research, Volume 2021, Article ID 7792282. doi: 10.1155/2021/7792282
Panwar J, Tarafdar JC. 2006. Distribution of three endangered medicinal plant species and their colonization with arbuscular mycorrhizal fungi. J Arid Environ. 65:337-350
Schneck NC, Y Perez.1988. Manual for the identification of VA Mycorrhizal Fungi. 2nd edition. INVAM & University of Florida. Florida.
Powell JR, Rillig MC. 2018. Biodiversity of arbuscular mycorrhizal fungi and ecosystem function. New Phytol 220:1059–1075. doi: 10.1111/nph.15119
Radi? T, Han?evi? K, Likar M, Regvar M, Zduni? G. 2018. High incidence of arbuscular mycorrhizal fungi in rare and endangered wild grapevine, Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 152:5, 1075-1078. doi: 10.1080/11263504.2017.1415994
Riaz M, Kamran M, Fang Y, Wang Q, Cao H, Yang G, Deng L, Wang Y, Zhou Y, Anastopoulos I, Wang X. 2021. Arbuscular mycorrhizal fungi-induced mitigation of heavy metal phytotoxicity in metal contaminated soils: A critical review. Journal of Hazardous Materials, 402: 123919
Ruotsalainen AL, Vare H, Vestberg M. 2002. Seasonality of root fungal colonization in low-alpine herbs. Mycorrhiza 12:29–36
Schüßler A, Walker C. 2010. The Glomeromycota. A species list with new families and new genera. Kew: The Royal Botanic Garden Kew
Schü?ler A, Schwarzott D, Walker C. 2001. A new fungal phylum, the Glomeromycota: Phylogeny and evolution. Mycol. Res. 105, 1413–1421
Sharma D, Rupan K, Bhatnagar AK. 2008. Arbuscular mycorrhizal (AM) technology for the conservation of Curculigo orchioides Gaertn.: an endangered medicinal herb. World J Microbiol Biotechnol 24:395-400
Shukla A, D Vyas, Anuradha J. 2013. Soil depth: an overriding factor for distribution of arbuscular mycorrhizal fungi. Journal of Soil Science and Plant Nutrition 13(1):23-33
Smith SE, Read DJ. 2008. Mycorrhizal symbiosis. Third ed. USA (ID) :Academic Press
Song J, Chen L, Chen F, Ye J. 2019. Edaphic and host plant factors are linked to the composition of arbuscular mycorrhizal fungal communities in the root zone of endangered Ulmus chenmoui Cheng in China. Ecology and Evolution. 9:8900–8910. doi: 10.1002/ece3.5446
Songachan LS, Kayang, H. 2012. Diversity and distribution of arbuscular mycorrhizal fungi in solanum species growing in natural condition. Agric Res (July–September 2012) 1(3):258–264. doi: 10.1007/s40003-012-0026-6
Spatafora JW, Chang Y, Benny GL, Lazarus K, Smith ME, Berbee ML, Bonito G, Corradi N, Grigoriev IV, Gryganskyi A et al. 2016. A phylum-level phylogenetic classification of zygomycete fungi based on genome-scale data. Mycologia, 108, 1028–1046
Stürmer SL, Bever JD, Morton JB. 2018. Biogeography of arbuscular mycorrhizal fungi (Glomeromycota): A phylogenetic perspective on species distribution patterns. Mycorrhiza, 28, 587–603
Suting EG, Devi O. 2021. Occurrence and diversity of arbuscular mycorrhizal fungi in trap cultures from limestone mining sites and un-mined forest soil of Mawsmai, Meghalaya. Trop Ecol 62, 525–537. doi: 10.1007/s42965-021-00144-7
Tedersoo L, Sánchez-Ramírez S, Kõljalg U, Bahram M, Döring M, Schigel DS, May T, Ryberg M, Abarenkov K. 2018. High-level classification of the fungi and a tool for evolutionary ecological analyses. Fungal Divers, 90, 135–159.
Trethowan, L. 2019. Kalappia celebica. The IUCN Red List of Threatened Species 2019: e.T33287A155605251. https://dx.doi.org/10.2305/IUCN.UK.2019-3.RLTS.T33287A155605251.en. Accessed on 22 April 2022
Tuheteru FD, Husna, Albasri, Arif A, Kramadibrata K, Soka, G. 2020. Composition and diversity of arbuscular mycorrhizal fungi spore associated with different land-use types in tropical gold mine. J. Degrade. Min. Land Manage., 8 (1): 2503-2512. doi: 10.15243/jdmlm.2020.081.2503
Turjaman M, Santosa E, Sumarna Y. 2006b. Arbuscular mycorrhizal fungi increased early growth of gaharu wood species Aquilaria malaccensis and A. crasna under greenhouse conditions. J. For. Res. 3(2):139-148
Turjaman M, Tamai Y, Santoso E, Osaki M, Tawaraya K. 2006a. Arbuscular mycorrhizal fungi incresead early growth of two nontimber forest product species Dyera polyphylla and Aquilaria filaria under greenhouse conditions. Mycorrhiza. 16:459-464.
Zubek S, Turnau K, Tsimilli-Michael M, Strasser RJ. 2009. Response of endangered plant species to inoculation with arbuscular mycorrhizal fungi and soil bacteria. Mycorrhiza. 19:113-123
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