Chromatography analysis, in vitro antioxidant and antibacterial activities of essential oil of Artemisia herba-alba Asso of Boussaâda, Algeria




Abstract. Kadri M, Yahia A, Goubi S, Mekhedmi NE, Selmane M, Chemsa AE. 2022. Chromatography analysis, in vitro antioxidant and antibacterial activities of essential oil of Artemisia herba-alba Asso of Boussaâda, Algeria. Biodiversitas 23: 4424-4431. Artemisia herba-alba Asso (Asteraceae family) is widespread in the semi-arid and arid steppes of North Africa. This plant is used for traditional treatment. The present study aims to investigate the chemical composition, antioxidant and antimicrobial activities of A. herba-alba essential oil found in southwest Algeria (Boussaâda region). Artemisia herba-alba essential oil was obtained by hydrodistillation method, and its chemical composition was identified by using GC/MS analysis. In addition, the antioxidant activity of the extracted essential oil was determined using the DPPH assay. The antimicrobial activity of the essential oil was determined by the agar disc diffusion method. The essential oil extracted from the aboveground portions by hydrodistillation was analyzed by GC/MS. 38 components were identified, making up 69.37% of the oil, the most important of which is thujone (9.875%), camphor (3.762%), cis-p-menthadien-1-of (3.572%), and isoborneol (2.334%). The observed IC50 values of the DPPH assay were 7.31 ± 0.088 mg/mL. On the other hand, this oil was active against all strains tested, this activity varied from 12.77 ± 0.510 mm on Listeria innocua CIP 74915. These results demonstrate that the plant tested could be a potential source of natural antioxidants and antimicrobial agents.


Abood S, Eichelbaum S, Mustafi S, Veisaga ML, López LA, Barbieri M. 2017. Biomedical Properties and Origins of Sesquiterpene Lactones, with a Focus on Dehydroleucodine. Natural Product Communications. 12(6): 995-1005.
Akrout A, Gonzalez LA, El Jani HJ, Madrid PC. 2011. Antioxidant and antitumor activities of Artemisia campestris and Thymelaeahirsuta from southern of Tunisia. J. Food and Chemical Toxicology. 49 (2): 342–347.
Akrout, A. 2004. Essential oil study of some pastoral plants from Matmata (south Tunisia). Cahiers Options Méditerranéennes (CIHEAM) 62: 289-292.
Arab Y, Sahin B, Ceylan O, Zellagui A, Olmez O, Kucukaydin S, Tamfu AN, Ozturk M, Gherraf N. 2022. Assessment of in vitro activities and chemical profiling of Senecio hoggariensis growing in Algerian Sahara, biodiversitas 23(7): 3498-3506 DOI: 10.13057/biodiv/d230724
Aqil F, Khan MSA, Owais M, Ahmad I. 2005. Effect of certain bioactive plant extracts on clinical isolates of ?-lactamase producing methicillin resistant Staphylococcus aureus. Journal of Basic Microbiology. 45 (2), 106–114.
Belhattab R, Amor L, Barroso JG, Pedro LG, Figueiredo AC. 2014. Essential oil from Artemisia herba-alba Asso grown wild in Algeria: Variability assessment and comparison with an updated literature survey. Arabian Journal of Chemistry 7(2): 243-251
Bertella A, Benlahcen KH, Abouamama S, Diana CGAP, Karim MK, Kihal M, Artur MSS. 2018. Artemisia herba-alba Asso. essential oil antibacterial activity and acute toxicity. Industrial Crops and Products. 116: 137-143
Bezza L, Mannarino A, Fattarsi K, Mikail C, Abou L, HadjiMinaglou F, Kaloustian J, 2010. Chemical composition of the essential oil of Artemisia herba-alba issued from the district of Biskra (Algeria). Phytothérapie. 8(5): 277-281. doi:10.1007/s10298-010-0576-3
Boukhris M, Regane G, Yangui T, Sayadi S, Bouaziz M. 2012. “Chemical composition and biological potential of essential Oil from Tunisian Cupressus sempervirens L. Journal of Arid Land Studies. 22 (1): 329–332.
Blois MS. 1958. Antioxidant Determination By The Use of a Stable Free Radical,” NATURE, 181 :1199-1200. Http://Dx.Doi.Org/10.1038/1811199a0
Bruneton J. 1999 . Pharmacognosie. Phytochimie. Plantes médicinales. 3ème édition. éd. TEC et DOC. Paris. 3 ème édition : Editions TEC & DOC Lavoisier, 1120p
Carson CF, Hammer KA, Riley TV. 1995. Broth micro-dilution method for determining the susceptibility of Escherichia coli and Staphylococcus aureus to the essentia1 oil of Melaleuca alternifolia (tea tree oil). Microbios 82: 181-185.
Chalchat JC, Garry RPH, Harama M, Sidibe L.1995. Plantes aromatiques du Mali. Etude de deux Ocimum : O. basilicum et O. canum Sims. RivistaItaliana EPPOS. 204-214.
Cosentino S, Tuberoso CIG, Pisano B, Satta M , Mascia V, Arzedi E , Palmas F. 1999. in-vitro antimicrobial activity and chemical composition of Sardinia Thymus essential oils. Letter in Applied. Microbiology 29: 130-135.
Costa DC, Costa HS, Albuquerque TG, Ramos F, Castilho MC, Sanches-Silva A. 2015. Advances in phenolic compounds analysis of aromatic plants and their potential applications. Trends in Food Science & Technology. 45(2): 336–354.
Dahmani-Hamzaoui N, Baaliouamer A. 2010. Chemical composition of Algerian Artemisia herba-alba essential oils isolated by microwave and hydrodistillation. Journal of Essential Oil Research. 22(6): 514-517.
Djousse Bmk , Ngoune Nf, Djoukeng Hg, Nono Lw , Sogang Hs , Tangka Jk. 2022. Extraction et Caractérisation des Huiles Essentielles de Trois Plantes Aromatiques Cultivées à l’Ouest-Cameroun : Rosmarinus officinalis, Salvia officinalis et Cymbopogon citratus. Cameroon Journal of Biological and Biochemical Sciences 30( 2): 121-133
Dob T, Ben Abdelkader T. 2006. Chemical composition of the essential oil of Artemisia herba-alba asso grown in Algeria. J.Essen. Oil Res.18: 685-690.
El-massry K.F, El-ghorab AH, Farouk A. 2002. Antioxidant activity and volatile components of Egyptian Artemisia judaica L. Food chemistry .79: 331–336.
Elmastas M, Isildak O, Turkekul I, Temur N. 2007. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Composition and Analysis. 20, 337-345
Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M, Abdelly C. 2008. Phenolic composition of Cynara cardunculus L. organs, and their biological activities, Comptes Rendus Biologies. 331(5): 372-379.
Gergis V, Spilotis V, Poulos C. 1990. Antimicrobial activity of essential oils from Greek Sideritis species. Pharmazie, 45: 70-75
Ghanmi M, Satrani B, Aafi A, Ismaili MR, Houtia H, Manfalouti H, Benchakroun K, Abarchane M, Harki L, Boukir A, Chaouch A, Charrouf Z. 2010. Effet de la date de récolte sur le rendement, la composition chimique et la bioactivité des huiles essentielles de l’armoise blanche (Artemisia herba-alba) de la région de Guercif (Maroc oriental).Phytothérapie, 8(5), 295-301.
Griffin SG, Wyllie SG, Markham JL, Leach DN. 1999. The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour and Fragrance Journal 14: 322-332;2-4
Guinoiseau E. 2010. Molécules antibactériennes issues d’huiles essentielles : séparation, identification et mode d’action. Thèse de doctorat .Université de Corse. Ecole Doctorale Environnement et Société UMRCNRS 6134 SPE. 148p.
Harborne JB. 1998. Phytochemical methods. A guide to modern techniques of plants analysis. Third Edition. ISBN: 0-412-57260-5 (HB) and 0-412-57270-2 (PB).
Hatimi S, Boudouma M, Bichichi M, Chaib N, Idrissi NG. 2001. In vitro evaluation of antileishmania activity of Artemisia herba alba Asso. Bulletin de la société de pathologie exotique. 94(1), 29-31
Jain N, Sharma M. 2022. Comparative Analysis of Antidermatophytic Potential of Cymbopogon citratus Essential Oil Stored in Different Temperature Condition for Two Years. Pharmacog Res.14(3):241-5.
Kadri M, Salhi N, Yahia A , Amiar K , Gnabzia H. 2017. Chemical composition, antioxidant and antimicrobial activities from extracts of Cymbopogon schoenanthus L. (spreng) of Algeria. International Journal of Biosciences | IJB .10(1): 318-326.
Karousou R, Koureas DN, Kokkini S. 2005. Essential oil composition is related to the natural habitats: Coridothymus capitatus and Satureja thymbra in NATURA 2000 sites of Crete. Phytochemistry. 66(22): 2668-2673.
Lattaoui N, Tantaoui-elaraki A. 1994. Individual and combined antibacterial activity of the main components of three thyme essential oils. RevistaItaliana Eppos 13: 13-19.
Lopes-Lutz D, Alviano D, Alviono CS, Kolodziejczyk PP. 2008. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry. 69: 1732-1738.
Mouhamed Abou El-Hamd H , Magdi AE, Mohamed EH , Soleiman EH , Abeer ME, Naglaa SM. 2010. Chemical Constituents and Biological Activities of Artemisia herba-alba. Records of Natural Products . 4(1): 1-25
Phaiphan A, Baharin BS, Tan CP, Abdul Rahman R, Ganesan P. 2014. Antioxidant and antibacterial activities of different solvent extractions from Cassia siamea (Lamk.) leaves . Journal of Chemical and Pharmaceutical Research. 6(4):655-662
Saad S, Ouafi S, Karoune S, Meguellati H, Djemouai N, Harchaoui L.2022. Marrubium deserti De Noé, a Spontaneous Medicinal Plant from Algerian Sahara: An Ethnobotanical Survey. Journal Algérien des Régions Arides, 14 (2) : 110–122.
Schinor EC, Salvador MJ, Ito IY, Dias DA. 2007. Evaluation of the antimicrobial activity of crude extracts and isolated constituents from Chrestascapigera. Brazilian Journal of Microbiology. 38: 145-149.
Shimada K, Fujikawa K, Yahara K, Nakamura T. 1992.Antioxidative properties of xanthone on the auto oxidation of soybean in cylcodextrin emulsion. Journal of agricultural and food chemistry (USA). 40: 945–948.
Shirwaikar A, Prabhu KS, Punitha ISR. 2006. In vitro antioxidant studies of phaeranthusindicus (Linn Indian Journal of Experimental Biology (IJEB). 44: 993-996.
Sikkema J, De Bont JAM, Poolman B. 1994. Interactions of cyclic hydrocarbons with biological membranes. Journal of Biological Chemistry. 269(11): 8022-8028.
Singh A, Pratap-Singh A, Ramaswamy HS. 2016. A Controlled Agitation Process for Improving Quality of Canned Green Beans during Agitation Thermal Processing. Journal of Food Science. 81(6): 1399–E1411.
Teffane M, Boudries H, Bachir Bey M, Kadi A, Boukhalfa F. 2021. Effect of Solvent Type, Extraction Temperature, Agitation Speed and Microwave Power on Phenolic Compound Extraction and Antioxidant Activity of Apricot Kernels (Prunus armeniaca L.). Current Bioactive Compounds, 17: 1-00. DOI: 10.2174/1573407217666210215085507
Trease E, Evans WC. 1987.Pharmacognosie. Billiairetindall, london 13 th edition. p: 61-62.
Wang WM. 2004. On the Origin and Development of Artemisia (Asteraceae) in the Geological Past. Botanical Journal of the Linnean Society. 145(3), 331–336.
Zaim A, El Ghadraoui L, Farah A. 2012. Effets des huiles essentielles d’Artemisia herba-alba sur la survie des criquets adultes d’Euchorthippus albolineatus (Lucas, 1849). Bulletin de l’Institut Scientifique, Rabat, section Sciences de la Vie. 34(2): 127-133.
Zhang H, Chen F, Wang X, Yao HY.2006. Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International. 39, 833- 839.