Molecular detection of extended spectrum ?-lactamase producing Klebsiella pneumoniae from wild deer
##plugins.themes.bootstrap3.article.main##
Abstract
Abstract. Riwu KHP, Rantam FA, Effendi MH, Khairullah AR, Widodo A, Kurniawan SC, Ugbo EN. 2022. Molecular detection of extended spectrum ?-lactamase producing Klebsiella pneumoniae from wild deer. Biodiversitas 23: 4256-4262. Antimicrobial resistance (AMR) has become a severe threat worldwide and its remote cause remains unclear in most parts due to the global emergence of new resistance mechanisms by microorganisms. This research aimed to identify the presence of extended-spectrum ?-lactamase (ESBL) Klebsiella pneumoniae from wild deer feces in several places in East Java and East Nusa Tenggara. This study used 129 fresh feces samples collected from wild deer and analyzed for the presence of K. pneumoniae using standard microbiological techniques. An antibiotic sensitivity test was performed using Kirby-Bauer Disc Diffusion Method. The isolates were confirmed to the ESBL producer by double disk test and ESBL genes were identified by PCR. Nine samples (6.98%) were K. pneumoniae positive out of the 129 wild deer fresh feces. Among the nine K. pneumoniae isolates studied 3 (33.33%) were multidrug resistant, East Java (1 isolate) and East Nusa Tenggara (2 isolates). All the multidrug-resistant K. pneumoniae isolates were ESBL positive and also harbored blaTEM gene. The presence of MDR, and ESBL-producing K. pneumoniae isolates was confirmed in wild deer and this explained that wild deer should be considered a source of transmission of MDR and ESBL to public health.
##plugins.themes.bootstrap3.article.details##
Most read articles by the same author(s)
- DIAN AYU PERMATASARI, ADIANA MUTAMSARI WITANINGRUM, FRESHINTA JELLIA WIBISONO, MUSTOFA HELMI EFFENDI, Detection and prevalence of multidrug-resistant Klebsiella pneumoniae strains isolated from poultry farms in Blitar, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 10 (2020)
- MUSTOFA HELMI EFFENDI, WIWIEK TYASNINGSIH, YEMIMA ANGGUN YURIANTI, JOLA RAHMAHANI, NENNY HARIJANI, HANI PLUMERIASTUTI, Presence of multidrug resistance (MDR) and extended-spectrum beta-lactamase (ESBL) of Escherichia coli isolated from cloacal swab of broilers in several wet markets in Surabaya, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 1 (2021)
- SANCAKA CHASYER RAMANDINIANTO, ASWIN RAFIF KHAIRULLAH, MUSTOFA HELMI EFFENDI, MecA gene and methicillin-resistant Staphylococcus aureus (MRSA) isolated from dairy farms in East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 8 (2020)
- RIBBY ANSHARIETA, SANCAKA CHASYER RAMANDINIANTO, MUSTOFA HELMI EFFENDI, HANI PLUMERIASTUTI, Molecular identification of blaCTX-M and blaTEM genes encoding extended-spectrum ß-lactamase (ESBL) producing Escherichia coli isolated from raw cow’s milk in East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 22 No. 4 (2021)
- FRESHINTA JELLIA WIBISONO, BAMBANG SUMIARTO, TRI UNTARI, MUSTOFA HELMI EFFENDI, DIAN AYU PERMATASARI, ADIANA MUTAMSARI WITANINGRUM, Short Communication: The presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli on layer chicken farms in Blitar Area, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 21 No. 6 (2020)
- ASWIN RAFIF KHAIRULLAH, SRI AGUS SUDJARWO, MUSTOFA HELMI EFFENDI, SANCAKA CASHYER RAMANDINIANTO, MARIA AEGA GELOLODO, AGUS WIDODO, KATTY HENDRIANA PRISCILIA RIWU, DYAH AYU KURNIAWATI, SAIFUR REHMAN, Profile of Multidrug Resistance and Methicillin-Resistant Staphylococcus aureus (MRSA) on dairy cows and risk factors from farmer , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 6 (2022)
- FIDI NUR AINI EKA PUJI DAMEANTI, SHEILA MARTY YANESTRIA, AGUS WIDODO, MUSTOFA HELMI EFFENDI, HANI PLUMERIASTUTI, WIWIEK TYASNINGSIH, RAHAYU SUTRISNO, M. ALI AKRAMSYAH, Incidence of Escherichia coli producing Extended-spectrum beta-lactamase in wastewater of dairy farms in East Java, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 24 No. 2 (2023)
- MUSTOFA HELMI EFFENDI, HAYYUN DURROTUL FARIDAH, FRESHINDY MARISSA WIBISONO, FRESHINTA JELLIA WIBISONO, NABILATUN NISA, FATIMAH, EMMANUEL NNABUIKE UGBO, Detection of virulence factor encoding genes on Escherichia coli isolated from broiler chicken in Blitar District, Indonesia , Biodiversitas Journal of Biological Diversity: Vol. 23 No. 7 (2022)