Assessment of state of Dactylorhiza fuchsii (Orchidaceae) populations from the Altai mountains of Kazakhstan




Abstract. Sumbembayev AA, Tergenbaeva ZHT, Kudabayeva GM, Tashmetova RS, Genievskaya YA, Szlachetko DL. 2022. Title. Biodiversitas 23: 4385-4399. The article presents the results of studying the current state of populations of a rare species - Dactylorhiza fuchsii in the Kazakhstan Altai. As a result of expeditionary studies, four main regions were identified, which were represented by 12 populations. Floristic plasticity according to the Jaccard coefficient (12-24%) showed a high heterogeneity of the studied populations. The cluster population dendrogram showed a clear dependence of the floristic composition of accompanying species on geographic location in terms of species similarity and difference of communities. External similarity was studied according to 10 morphometric features. The PCA analysis shows that the external structure is influenced by vertical zoning. Pearson's correlation analysis showed a stable direct and inverse dependence of morphometric characteristics on some environmental conditions (0.74-0.95). According to the data ANOVA disperse analysis, among studied environmental factors, soil nutrient richness (N) and humidity (F) had demonstrated the broadest significant effects (P-values from < 2e-16 to 0.001 for N and from 8.02e-09 to 0.02 for F) on plants morphology influencing eight out of ten morphological traits. Distribution regions of D. fuchsii have low vitality, and most populations are characterized as depressed. The results harmoniously complement the fundamental research on the state of D. fuchsii populations throughout the distribution range of the species.


Aybeke M., Sezik E., Olgun G. (2010) Vegetative anatomy of some Ophrys, Orchis and Dactylorhiza (Orchidaceae) taxa in Trakya region of Turkey. Flora 205:73–89
Bajtulin I.O., Kotukhov Ju.A. (2011) Flora sosudistyh rastenij Kazahstanskogo Altaja [Flora of vascular plants of Kazakhstan Altai], Almaty [in Russian].
Bateman, R. M., & Denholm, I. (1989). A reappraisal of the British and Irish dactylorchids, 3. The spotted-orchids. Watsonia, 17, 319-349.
Box M. S. et al. (2008) Floral ontogenetic evidence of repeated speciation via paedomorphosis in subtribe Orchidinae (Orchidaceae). Botanical Journal of the Linnean Society. Vol. 157: ?. 3, 429-454.
Box, M. S., Dodsworth, S., Rudall, P. J., Bateman, R. M., & Glover, B. J. (2012). Flower-specific KNOX phenotype in the orchid Dactylorhiza fuchsii. Journal of experimental botany, 63(13), 4811-4819.
Braun-Blanquet, J. P., & Schoenichen, W. (1964). Grundzuge der vegetationskunde. Auf Wien, 865.
Danilova A.N., Sumbembayev A.A. The status of the Dactylorhiza incarnata populations in the Kalba Altai, Kazakhstan // Biodiversitas. Journal of Biological Diversity. – 2021. – Vol. 22. – ?. 8. 3180-3195.
De Hert, K., Jacquemyn, H., Van Glabeke, S., Roldan-Ruiz, I., Vandepitte, K., Leus, L., & Honnay, O. (2012). Reproductive isolation and hybridization in sympatric populations of three Dactylorhiza species (Orchidaceae) with different ploidy levels. Annals of Botany, 109(4), 709-720.
?or?evi?, V., Jovanovi?, S., & Stevanovi?, V. (2014). Dactylorhiza fuchsii (Orchidaceae), a new species in the flora of Serbia. Archives of Biological Sciences, 66(3), 1227-1232.
Dufrêne, M., Gathoye, J. L., & Tyteca, D. (1991). Biostatistical studies on western European Dactylorhiza (Orchidaceae)—the D. maculata group. Plant Systematics and Evolution, 175(1), 55-72.
Efimov, P. G., Philippov, E. G., & Krivenko, D. A. (2016). Allopolyploid speciation in Siberian Dactylorhiza (Orchidaceae, Orchidoideae). Phytotaxa, 258(2), 101-120.
Egorova N.Yu., Suleimanova V.N. 2021. Variability of morphological structures in Cypripedium calceolus (Orchidaceae) and their relationship with the habitat conditions in the southern taiga ecosystems, Russia. Nature Conservation Research 6(1): 28–41.
Electronic resource: Physical geography of Kazakhstan (
Gutowski, J. M. (1990). Pollination of the orchid Dactylorhiza fuchsii by longhorn beetles in primeval forests of Northeastern Poland. Biological Conservation, 51(4), 287-297.
Ishbirdin A.R., Ishmuratova M.M. Adaptive morphogenesis and ecological-coenotic strategies for the survival of herbaceous plants // Methods of population biology. Collection of materials VII All-Russian. Population Seminar (Syktyvkar, February 16–21, 2004). Syktyvkar, 2004, part 2, pp. 113–120.
IUCN. 2020. The IUCN Red List of Threatened Species. (
Jaccard P. (1901) Distribution de la flore alpine dans le Bassin des Dranses et dans quelques regions voisines Bull. Soc. Vaudoise sci. Natur. 37. Bd. 140., 241—272.
Jacquemyn H, Deja A, De hert K, Cachapa Bailarote B, Lievens B (2012) Variation in Mycorrhizal Associations with Tulasnelloid Fungi among Populations of Five Dactylorhiza Species. PLoS ONE 7(8): e42212.
Jakobsone, G. (2008). Morphogenesis of wild orchid Dactylorhiza fuchsii in tissue culture. Acta Universitatis Latviensis, Biology, 745, 17-23.
Jane?ková, P., Wotavová, K., Schödelbauerová, I., Jersáková, J., & Kindlmann, P. (2006). Relative effects of management and environmental conditions on performance and survival of populations of a terrestrial orchid, Dactylorhiza majalis. Biological Conservation, 129(1), 40-49.
Kami?ska E., Miernicka I., Mroz I., Sarosiek J. (1990) Ecological organization of the population of Dactylorhiza fuchsii (Druce) Soo. Acta Univ Wratislawiensis. 1055, 77–93.
Kirillova, I. A., & Kirillov, D. V. (2013). Features of biology, reproduction and structure of cenopopulations of Dactylorhiza fuchsii sl (Orchidaceae) at the northern limit of distribution. Botanicheskij zhurnal, 98 (2), 75-90.
Kosolapova, N. V., Gudovskikh, Y. V., Egoshina, T. L., Kislitsyna, A. V., & Luginina, E. A. (2021, November). To the Characteristic Of Cenopopulations Dactylorhiza Dactylorhiza Fuchsii (Druce) Soo Of The Middle And Southern Taiga. In IOP Conference Series: Earth and Environmental Science (Vol. 901, No. 1, p. 012025). IOP Publishing.
Kotukhov, Y. A., Danilova, A. N., Anufriyeva, O. A., Suleimenov, A. N., Sumbembayev, A. A., & Kubentaev, S. A. (2018). Ecological and biological features of Cypripedium at Katon-Karagay State National Natural Park. Plant Archives, 18(2), 1499-1502.
Kupriyanov, O. A., Kupriyanov, A. N., Abduova, A. A., Yessengeldi, A., Sataev, M. I., Moshkalov, B. M., & Tolegen, M. (2019). Coenoflora and Spiraeanthus schrenkianus Maxim population structure in the mountains of Boraldaytau. EurAsian Journal of BioSciences, 13(2), 1205-1213.
Landolt E. (1977). Okologische Zeigerwerte zur Schweizer Flora. Veroff. Geobot. Inst. Rubel. H.64. Zurich: Stiftung Riibel. 208 pp.
Naczk, A. M., Kowalkowska, A. K., Wi?niewska, N., Hali?ski, ?. P., Kapusta, M., & Czerwicka, M. (2018). Floral anatomy, ultrastructure and chemical analysis in Dactylorhiza incarnata/maculata complex (Orchidaceae). Botanical Journal of the Linnean Society, 187(3), 512-536.
Peakall, R., & Smouse, P. E. (2012). GenAlEx tutorials-part 2: genetic distance and analysis of molecular variance (AMOVA). Bioinformatics, 28, 2537-2539.
Pearson, Karl (20 June 1895). "Notes on regression and inheritance in the case of two parents". Proceedings of the Royal Society of London. 58: 240- 242.
Peet, R. K., Wentworth, T. R., & White, P. S. (1998). A Flexible, Multipurpose Method for Recording Vegetation Composition and Structure. Castanea, 63(3), 262–274.
Perezhogin Ju.V. (2017) Ujazvimye jelementy flory Kostanajskoj oblasti [Vulnerable elements of the flora of Kostanay region] Biologicheskoe raznoobrazie aziatskih stepej: Materialy III mezhdunar. nauchn. konf. (Kostanaj, Kazahstan) – Biological diversity of the Asian steppes: Proceedings of the III Intern. scientific. conf. / pod nauchn. redakciej E.A. Abil', T.M. Braginoj. - Kostanaj: KGPI., 366. [in Russian].
POWO (2022). "Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet; 15 January 2022."
Red Book of Kazakhstan. (2014). Ed. 2nd, revised and enlarged. Volume 2: Plants (team of authors). Astana: LLP "ArtPrint, 21. 452 pp. (in Russian).
Spearman, C., American Journal of Psychology 15: 72 (1904).
Sultangazina G.Zh., Hrustaleva I.A., Kuprijanov A.N. (2013) Redkie rastenija nacional'nogo prirodnogo parka «Burabaj» Vestnik KazNU. [Rare plants of the national natural park "Burabay"] Vestnik KazNU ser. Jekol. – KazNU Bulletin. ser. Ekol. ?3(39), 264-270. [in Russian]
Sumbembayev, A., Abugalieva, S., Danilova, A., Matveyeva, E., & Szlachetko, D. (2021). Flower morphometry of members of the genus Dactylorhiza Necker ex Nevski (Orchidaceae) from the Altai Mountains of Kazakhstan. Biodiversitas Journal of Biological Diversity, 22(8).
Takhtajan A.I. (2009) Flowering plants. 2 ed., 871 p.
Taraška, V., Batoušek, P., Duchoslav, M., Temsch, E. M., Weiss-Schneeweiss, H., & Trávní?ek, B. (2021). Morphological variability, cytotype diversity, and cytogeography of populations traditionally called Dactylorhiza fuchsii in Central Europe. Plant Systematics and Evolution, 307(4), 1-21.
Tyteca, D., & Gathoye, J. L. (1999). Morphometric analysis of Dactylorhiza occitanica and related populations from eastern France (Orchidaceae). Belgian Journal of Botany, 158-174.
Vahrameeva M.G., Varlygina T.I., Tatarenko I.V. (2014). Orhidnye Rossii (biologija, jekologija i ohrana). [Orchids of Russia (biology, ecology and conservation)] Moscow [in Russian]
Vakhrameeva, M. G. (2006). Ontogeny and population dynamics of Dactylorhiza fuchsii (Orchidaceae). Botanicheskij zhurnal, 91(11), 1683-1695.
Wróblewska, A., Szczepaniak, L., Bajguz, A., J?drzejczyk, I., Ta?a?aj, I., Ostrowiecka, B., ... & Mirski, P. (2019). Deceptive strategy in Dactylorhiza orchids: multidirectional evolution of floral chemistry. Annals of botany, 123(6), 1005-1016.
Zlobin Yu.A. Principles and methods of coenotic plant populations. Kazan: Kazan University, 1989. 146 p.

Most read articles by the same author(s)