First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes
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Abstract. Sibero MT, Frederick EH, Sabdono A, Wijayanti DP, Pringgenies D, Radjasa OK, Zilda DS, Murwani R. 2022. First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes. Biodiversitas 23: 1408-1419. Yeast has been widely utilized in various industries due to its enzyme properties. Therefore, plenty of studies focus on exploring yeast biodiversity from diverse sources. However, there are limited reports regarding marine yeast biodiversity and its potential from Indonesia. This study aimed to isolate and identify the seaweed-associated yeast from Jepara in Central Java and Sepanjang Beach in Gunung Kidul, Yogyakarta, then examined their potential to produce extracellular polysaccharides-degrading enzymes (EPEs). Marine yeasts were isolated using standard marine agar (STD) and potato dextrose agar (PDA). All isolates were characterized by their salinity tolerance, morphology, and species confirmation using DNA barcoding. Three EPEs consisting of agarase, alginate-lyase, and carrageenase were screened using polysaccharides-enriched agar media. A specific agarase encoding gene was detected with specific primers. In total, 21 seaweed-associated yeast were successfully isolated from 6 seaweeds and noted as facultative marine yeast. The DNA barcoding study discovered that these yeasts belonged to 5 genera, consisted of Aureobasidium, Candida, Debaryomyces, Hortaea, and Rhodotorula. Moreover, Candida was noted as the most abundant genus (71.42%). It was noted that Hortaea werneckii MTJ.11 and MTJ.13 were positive for all enzymes; Aureobasidium melanogenum MTGK.31 gave a positive result for agarase and carrageenase; while [Candida] zeylanoides MTGK.23 only exhibited alginate-lyase activity. Unfortunately, none of the primers used to detect the presence of genes encoding polysaccharide-degrading enzymes were successfully amplified.
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References
Abdel-Raheem A, Shearer CA. 2002. Extracellular enzyme production by freshwater ascomycetes. Fungal Diversity 11: 1–19.
Aljohani R, Samarasinghe H, Ashu T, Xu J. 2018. Diversity and relationships among strains of culturable yeasts in agricultural soils in Cameroon. Scientific Reports 8(1): 1–11.
Amoikon TLS, Aké MDF, Djéni NT, Grondin C, Casaregola S, Djè KM. 2019. Diversity and enzymatic profiles of indigenous yeasts isolated from three types of palm wines produced in Côte d’Ivoire. Journal of Applied Microbiology 126(2): 567–579.
Ariño J, Ramos J, Sychrová H. 2010. Alkali metal cation transport and homeostasis in yeasts. Microbiology and Molecular Biology Reviews 74(1): 95–120.
Ayuningtyas EP, Sibero MT, Hutapea NEB, Frederick EH, Murwani R, Zilda DS, Wijayanti DP, Sabdono A, Pringgenies D, Radjasa OK. 2021. Screening of Extracellular Enzyme from Phaeophyceae-Associated Fungi; Proceedings of the sixth international conference on tropical and coastal region eco-development. Universitas Diponegoro. Central Java, 27-28 October 2020. [Indonesia]
Barnett JA. 2003. Beginnings of microbiology and biochemistry: The contribution of yeast research. Microbiology 149(3): 557–567.
Becker M, Lütz S, Rosenthal K. 2021. Environmental assessment of enzyme production and purification. Molecules 26(3): 1-10.
Bhagobaty RK, Joshi SR. 2012. Enzymatic activity of fungi endophytic on five medicinal plant species of the pristine sacred forests of meghalaya, India. Biotechnology and Bioprocess Engineering 17(1): 33–40.
Branda E, Turchetti B, Diolaiuti G, Pecci M, Smiraglia C, Buzzini P. 2010. Yeast and yeast-like diversity in the southernmost glacier of Europe (Calderone Glacier, Apennines, Italy). FEMS Microbiology Ecology 72(3): 354–369.
Bruno S, Coppola D, Di Prisco G, Giordano D, Verde C. 2019. Enzymes from marine polar regions and their biotechnological applications. Marine Drugs 17(10): 1–36.
Buzzini P, Lachance MA, Yurkov A. 2017. Yeasts in Natual Ecosystems: Diversity. Springer Nature, Switzerland.
Calabon MS, Sadaba RB, Campos WL. 2018. Fungal Diversity of Mangrove-associated Sponges from New Washington, Aklan, Philippines. Mycology 10(1): 1–16.
Carrasco M, Rozas JM, Barahona S, Alcaíno J, Cifuentes V, Baeza M. 2012. Diversity and extracellular enzymatic activities of yeasts isolated from King George Island, the sub-Antarctic region. BMC Microbiology 12: 1-9.
Chand K, Kumar S, Asfaq, Saxena D. 2016. Fermented foods and their health benefits. European Journal of Biotechnology and Bioscience 4(9): 2–4.
Chasani AR, Suyono EA. 2020. Comparison of structure and composition of seaweeds population in Porok and Greweng coasts, Gunungkidul, Indonesia. Proceedings of the sixth international conference on biological science. Gadjah Mada University. Yogyakarta, 10-11 October 2019. [Indonesia]
Coppejans E, Prathep A, Lewmanomont K, Hayashizaki K, De Clerck O, Leliart F, Terada R. 2017. Seaweeds and Seagrasses of the Southern Andaman Sea coast of Thailand. Kagoshima University Museum, Kagoshima.
Davani-Davari D, Negahdaripour M, Karimzadeh I, Seifan M, Mohkam M, Masoumi SJ, Berenjian A, Ghasemi Y. 2019. Prebiotics: Definition, types, sources, mechanisms, and clinical applications. Foods 8(3): 1–27.
Erlania E, Radiarta IN. 2017. Observation of Wild Seaweed Species in Labuhanbua Waters, Indonesia: a preliminary assessment for aquaculture development. Omni-Akuatika 13(1): 13-25.
Francis MM, Webb V, Zuccarello GC. 2016. Marine yeast biodiversity on seaweeds in New Zealand waters. New Zealand Journal of Botany 54(1): 30–47.
Gal-Hemed I, Atanasova L, Komon-Zelazowska M, Druzhinina IS, Viterbo A, Yarden, O. 2011. Marine isolates of Trichoderma spp. As potential halotolerant agents of biological control for arid-zone agriculture. Applied and Environmental Microbiology 77(15): 5100–5109.
Gomez EJ, Delgado JA, Gonzalez JM. 2020. Environmental factors affect the response of microbial extracellular enzyme activity in soils when determined as a function of water availability and temperature. Ecology and Evolution 10(18): 10105–10115.
Gurpilhares D de B, Cinelli LP, Simas NK, Pessoa A, Sette LD. 2019. Marine prebiotics: Polysaccharides and oligosaccharides obtained by using microbial enzymes. Food Chemistry 280: 175–186.
He M, Guo M, Zhang X, Chen K, Yan J, Irbis C. 2018. Purification and characterization of alginate lyase from Sphingomonas sp. ZH0. Journal of Bioscience and Bioengineering 126(3): 310–316.
Hohmann S. 2002. Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiology and Molecular Biology Reviews 66(2): 300–372.
Hou Y, Chen X, Chan Z, Zeng R. 2015. Expression and characterization of a thermostable and pH-stable ?-agarase encoded by a new gene from Flammeovirga pacifica WPAGA1. Process Biochemistry 50(7): 1068–1075.
Hutapea NEB, Sibero MT, Ayuningtyas EP, Frederick EH, Wijayanti DP, Sabdono A, Pringgenies D, Radjasa OK, Zilda DS, Murwani R. 2021. Seaweed-Associated Fungi from Sepanjang Beach, GunungKidul, Yogyakarta as Potential Source of Marine Polysaccharides-Degrading Enzymes; Proceedings of the sixth international conference on tropical and coastal region eco-development. Universitas Diponegoro. Central Java, 27-28 October 2020. [Indonesia]
Jamili, Yanti NA, Susilowati PE. 2016. Diversity and the role of yeast in spontaneous cocoa bean fermentation from Southeast Sulawesi, Indonesia. Biodiversitas 17(1): 90–95.
Ji J, Wang L, Wu H, Luan HM. 2010. Bio-function Summary of Marine Oligosaccharides. International Journal of Biology 3(1): 74-86.
Joseph I, Raj RP. 2007. Isolation and Characterization of Phytase Producing Bacillus strains from Mangrove Ecosystem. Journal of the Marine Biological Association of India 49(2): 177–182.
Jun Y, Zhang Y, Lemian L, Changfu W, Xiaoqing Y. 2012. Microbial Community and Urban Water Quality. Bulletin of the Chinese Academy of Sciences BCAS 26(1): 76-83.
Jutur PP, Nesamma AA, Shaikh KM. 2016. Algae-derived marine oligosaccharides and their biological applications. Frontiers in Marine Science 3: 1–5.
Kaewkrajay C, Chanmethakul T, Limtong S. 2020. Assessment of diversity of culturable marine yeasts associated with corals and zoanthids in the Gulf of Thailand, south China sea. Microorganisms 8(4): 1-16.
Kandasamy K, Alikunhi NM, Subramanian M. 2012. Yeasts in marine and estuarine environments. Journal of Yeast and Fungal Research 3(6): 74–82.
Kang HK, Seo CH, Park Y. 2015. The effects of marine carbohydrates and glycosylated compounds on human health. International Journal of Molecular Sciences 16(3): 6018–6056.
Kurtzman CP, Fell JW, Boekhout T. 2011. The Yeasts, a Taxonomic Study, 5th ed. Elsevier: USA.
Kutty SN, Philip R. 2008. Marine Yeasts - A Review. Yeast 25: 465–483.
Lee S, Park MS, Lee H, Kim JJ, Eimes JA, Lim YW. 2019. Fungal Diversity and Enzyme Activity Associated with the Macroalgae, Agarum clathratum. Mycobiology 47(1): 50–58.
Ludwig JA, Reynolds JF. 1988. Statistical Ecology: A Primer on Methods and Computing. John Wiley & Sons, USA.
Mukaremera L, Lee KK, Mora-Montes HM, Gow NAR. 2017. Candida albicans yeast, pseudohyphal, and hyphal morphogenesis differentially affects immune recognition. Frontiers in Immunology 8(629): 1–12.
Nagahama T, Hamamoto M, Nakase T, Takami H, Horikoshi K. 2001. Distribution and identification of red yeasts in deep-sea environments around the northwest Pacific Ocean. Antonie van Leeuwenhoek 80: 101–110.
Nagano Y, Nagahama T, Hatada Y, Nunoura T, Takami H, Miyazaki J, Takai K, Horikoshi K. 2010. Fungal diversity in deep-sea sediments - the presence of novel fungal groups. Fungal Ecology 3(4): 316–325.
Novak Babi? M, Zalar P, Ženko B, Džeroski S, Gunde-Cimerman N. 2016. Yeasts and yeast-like fungi in tap water and groundwater, and their transmission to household appliances. Fungal Ecology 20: 30–39.
O’Sullivan L, Murphy B, McLoughlin P, Duggan P, Lawlor PG, Hughes H, Gardiner GE. 2010. Prebiotics from marine macroalgae for human and animal health applications. Marine Drugs 8(7): 2038–2064.
Pielou EC. 1966. The Measurement of Diversity in Different Types of Biological Collections. J. Theoret. Biol. 13: 131-144.
Putri AC, Nugroho IC, Firdaus NUN, Puspita NOJ, Fajrin SAR, Hamzah SDA. 2017. Diversity and Abundance of Chlorophyta in Krakal Beach, Gunung Kidul, Yogyakarta. Proceedings of the second International Conference on Applied Marine Science and Fisheries Technology. Tual State Fishery Polytechnic. Southeast Maluku, 22-25 August 2017. [Indonesia]
Rahmawati YF. 2021. Macroinvertebrates Diversity in Sundak Beach, Gunung Kidul, Yogyakarta. Jurnal Biota 7(1): 36–41.
Raja HA, Miller AN, Pearce CJ, Oberlies NH. 2017. Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. Journal of Natural Products 80(3): 756–770.
Raveendran S, Parameswaran B, Ummalyma SB, Abraham A, Mathew AK, Madhavan A, Rebello S, Pandey A. 2018. Applications of microbial enzymes in food industry. Food Technology and Biotechnology 56(1): 16–30.
Ren Y, Bai Y, Zhang Z, Cai W, Del Rio Flores A. 2019. The preparation and structure analysis methods of natural polysaccharides of plants and fungi: A review of recent development. Molecules 24(17): 1-26.
Romdoni TA, Ristiani A, Meinita MDN, Marhaeni B, Setijanto. 2018. Seaweed species composition, abundance and diversity in drini and kondang merak beach, Java. E3S Web of Conferences 47(03006): 1–8.
Romo-Sánchez S, Alves-Baffi M, Arévalo-Villena M, Úbeda-Iranzo J, Briones-Pérez A. 2010. Yeast biodiversity from oleic ecosystems: Study of their biotechnological properties. Food Microbiology 27(4): 487–492.
Ruginescu R, Gomoiu I, Popescu O, Cojoc R, Neagu S, Lucaci I, Batrinescu-Moteau C, Enache M. 2020. Bioprospecting for novel halophilic and halotolerant sources of hydrolytic enzymes in brackish, saline and hypersaline lakes of romania. Microorganisms 8(12): 1–16.
Russo G, Libkind D, Sampaio JP, Van Broock MR. 2008. Yeast diversity in the acidic Rio Agrio-Lake Caviahue volcanic environment (Patagonia, Argentina). FEMS Microbiology Ecology 65(3): 415–424.
Sabdono A, Radjasa OK, Trianto A, Sibero MT, Martynov A, Kristiana R. 2021. an Ecological Assessment of Nudibranch Diversity Among Habitats Receiving Different Degrees of Sedimentation in Jepara Coastal Waters, Indonesia. International Journal of Conservation Science 12(1): 291–302.
Schlaberg R, Simmon KE, Fisher MA. 2012. A systematic approach for discovering novel, clinically relevant bacteria. Emerging Infectious Diseases 18(3): 422–430.
Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque A, Chen W, Consortium FB. 2012. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences of the United States of America 109(16): 6241–6246.
Schoch CL, Ciufo S, Domrachev M, Hotton CL, Kannan S, Khovanskaya R, Leipe D, Mcveigh R, O'Neill K, Robbertse B, et al. 2020. NCBI Taxonomy: a comprehensive update on curation, resources and tools. Database 2020: 1-21.
Shahat AS. 2017. production of extracellular hydrolytic enzymes by yeast grown on commercial media Production of Extracellular Hydrolytic Enzymes by Yeast Extracts on Some Commercial Media. Annals of Biological Research, 8(3): 12-20.
Sharma A, Sharma SC. 2017. Physiological Basis for the Tolerance of Yeast Zygosaccharomyces bisporus to Salt Stress. HAYATI Journal of Biosciences 24(4): 176–181.
Sibero MT, Triningsih DW, Radjasa OK Sabdono, A. and Trianto, A. (2016). Evaluation of Antimicrobial Activity and Identification of Yellow Pigmented Marine Sponge-Associated Fungi from Teluk Awur, Jepara, Central Java. Indonesian Journal of Biotechnology, 21(1): 1-11.
Sibero MT, Sahara R, Syafiqoh N, Tarman K. 2017. Antibacterial activity of red pigment isolated from coastal endophytic fungi against multi-drug resistant bacteria. Biotropia 24(2):161-172.
Sibero MT, Igarashi Y, Radjasa OK, Sabdono A, Trianto A, Zilda DS, Wijaya YJ. 2019. Sponge-associated fungi from a mangrove habitat in Indonesia: species composition, antimicrobial activity, enzyme screening and bioactive profiling. International Aquatic Research, 11(2): 173–186.
Sibero MT, Pribadi R, Larasati SJH, Calabon MS, Sabdono A, Subagiyo S, Frederick EH. 2021. Diversity of sponge-associated fungi from a mangrove forest in Kemujan Island, Karimunjawa National Park, Indonesia. Biodiversitas 22(12): 5695-5705.
Simpson BK, Rui X, Klomkao S. 2012. Enzymes in Food Processing. In: “Food Biochemistry and Food Processing.” Simpson, B.K., Nollet, L.M.L., Toldra, F., Benjakul, S., Paliyath, G. and Hui, Y.H. (Eds.). 2nd Ed. John Wiley & Sons, Inc., USA, pp. 181-206.
Singh P, Raghukumar C, Verma P, Shouche, Y. 2010. Phylogenetic diversity of culturable fungi from the deep-sea sediments of the Central Indian Basin and their growth characteristics. Fungal Diversity 40: 89–102.
Smith B, Wilson JB. 1996. A Consumer’s Guide to Evenness Indices. Oikos 76(1): 70-82.
Stanaszek-Tomal E. 2020. Environmental factors causing the development of microorganisms on the surfaces of national cultural monuments made of mineral building materials—review. Coatings 10(12): 1–19.
Stiger-Pouvreau V, Bourgougnon N, Deslandes E. 2016. Carbohydrates from Seaweeds. In: “Seaweed in Health and Disease Prevention.” Fleurence, J. and Levine, I. (Eds.). Elsevier, USA, pp. 223-274.
Sudaryatno. 2016 Drought Vulnerability Mapping with Geomorphological Approach in Yogyakarta Special Region (DIY) and Central Java. Proceedings of the second International Conference of Indonesian Society for Remote Sensing. MAPIN. Yogyakarta, 17-19 October 2016. [Indonesia]
Sumerta IN, Kanti A. 2018. Taxonomic Approach for Species Diversity of Yeasts and Yeasts-like Fungi through D1/D2 Region of Large Subunit Ribosomal DNA Sequences. Biosaintifika: Journal of Biology & Biology Education 10(1): 72–78.
Tan RX, Zou WX. 2001. Endophytes: A rich source of functional metabolites. Natural Product Reports 18(4): 448–459.
Tanaka M, Endo S, Kotake F, Al-Saari N, Amin AKMR, Feng G, Mino S, Doi H, Ogura Y, Hayashi T, et al. 2017. Correction: Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed. PLoS ONE 12(12): 1–12.
Thompson DS, Carlisle PL, Kadosh D. 2011. Coevolution of morphology and virulence in Candida species. Eukaryotic Cell 10(9): 1173–1182.
Utami S, Anggoro S, Soeprobowati TR. 2017. The diversity and regeneration of mangrove on Panjang Island Jepara Central Java. International Journal of Conservation Science 8(2): 289–294.
Veliz EA, Martínez-Hidalgo P, Hirsch AM. 2017. Chitinase-producing bacteria and their role in biocontrol. AIMS Microbiology 3(3): 689–705.
Walker G, Stewart G. 2016. Saccharomyces cerevisiae in the Production of Fermented Beverages. Beverages 2(4): 1-12.
Wang M, Chen L, Zhang Z. 2021. Potential applications of alginate oligosaccharides for biomedicine – A mini review. Carbohydrate Polymers 271: 1-4.
Widyartini DS, Widodo P, Susanto AB. 2017. Thallus variation of Sargassum polycystum from Central Java, Indonesia. Biodiversitas 18(3): 1004–1011.
Wijaya AP, Bondar KG, Frederick EH, Igarashi Y, Sibero MT. 2020. Identification of marine bacteria HPP.4A and HPP.T13 and its anticancer activity against P388 murine leukaemia cell; Proceedings of the international conference on fisheries and marine. Khairun University. North Maluku, 15-17 June 2020. [Indonesia]
Wijaya AP, Sibero MT, Zilda DS, Windiyana AN, Wijayanto A, Frederick EH, Murwani R, Wijayanti DP, Sabdono A, Pringgenies D, Radjasa OK. 2021. Preliminary Screening of Carbohydrase-Producing Bacteria from Chaetomorpha sp. In Sepanjang Beach, Yogyakarta, Indonesia; Proceedings of the sixth international conference on tropical and coastal region eco-development. Universitas Diponegoro. Central Java, 27-28 October 2020. [Indonesia]
Wulandari SR, Hutabarat S, Ruswahyuni R. 2015. Effect of Flow and Density Distribution Substrates against Seaweed Waterway Panjang Island in the West and South. Diponegoro Journal of Maquares Management of Aquatic Resources 4(3): 91–98.
Yurkov AM, Wehde T, Federici J, Schäfer AM, Ebinghaus M, Lotze-Engelhard S, Mittelbach M, Prior R, Richter C, Röhl O, Begerow D. 2016. Yeast diversity and species recovery rates from beech forest soils. Mycological Progress 15(8): 845–859.
Zaky AS, Tucker GA, Daw ZY, Du C. 2014. Marine yeast isolation and industrial application. FEMS Yeast Research 14(6): 813–825.
Zeng C, Zhang L, Miao S, Zhang Y, Zeng S, Zheng B. 2016. Preliminary characterization of a novel ?-agarase from Thalassospira profundimonas. SpringerPlus 5(1): 2–9.
Zhang C, Kim SK. 2010. Research and application of marine microbial enzymes: Status and prospects. Marine Drugs 8(6): 1920–1934.
Zhu B, Ning L. 2015. Purification and characterization of a new ?-carrageenase from the marine bacterium vibrio sp. Nj-2. Journal of Microbiology and Biotechnology 26(2): 255–262.
Zhu B, Ni F, Xiong Q, Yao Z. 2021. Marine oligosaccharides originated from seaweeds: Source, preparation, structure, physiological activity and applications. Critical Reviews in Food Science and Nutrition 61(1): 60–74.
Zilda DS, Yulianti Y, Sholihah RF, Subaryono S, Fawzya YN, Irianto HE. 2019. A novel Bacillus sp. Isolated from rotten seaweed: Identification and characterization alginate lyase its produced. Biodiversitas 20(4): 1166–1172.
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