Effects of nutrient solution and substrate on Limnocharis flava performance in hydroponic systems

Main Article Content

RIZKA NOVI SESANTI
EDI PURWANTO
SAMANHUDI
SUDADI

Abstract

Abstract. Sesanti RN, Purwanto E, Samanhudi, Sudadi. 2025. Effects of nutrient solution and substrate on Limnocharis flava performance in hydroponic systems. Asian J Agric 9: 590-597. Limnocharis flava is a promising aquatic vegetable for substrate-based hydroponic cultivation. However, the optimal combination of nutrient concentrations and substrate types to maximize growth under hydroponic conditions remains unclear. This study evaluated five nutrient solution concentrations (0, 0.8, 1.6, 2.4, and 3.2 dS m-¹) and three substrate types (volcanic sand, rice husk charcoal, and a 1:1 mixture) in a Randomized Complete Block Design (RCBD) with three replications. Nutrient concentrations of 0.8 and 1.6 dS m-¹ produced the highest plant height (28.48 cm and 28.20 cm), number of leaves (11.31 and 11.03), shoot dry weight (4.61 g and 4.74 g), root fresh weight (13.20 g and 12.43 g), and root dry weight (0.76 g and 0.74 g). Both lower and higher concentrations inhibited growth, indicating optimal thresholds for nutrient uptake. Chlorophyll a, b, and total chlorophyll contents were similar across the 0.8-3.2 dS m-¹ range, but consistently higher than the control. The leaf greenness index showed a significant interaction between nutrient concentration and substrate type, with the highest value at 2.4 dS m-¹ in the mixed substrate. A nutrient concentration of 1.6 dS m-¹ produced the best overall performance. Based on these results, a combination of 1.6 dS m-¹ nutrient solution and a 1:1 mixture of volcanic sand and rice husk charcoal is recommended for the cultivation of L. flava. These findings establish baseline guidelines for hydroponic cultivation of L. flava and highlight its potential to advance precision hydroponics for indigenous vegetables, thereby contributing to food security and sustainable agriculture.

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How to Cite

SESANTI, R. N. ., PURWANTO, E. ., SAMANHUDI, S., & SUDADI, S. (2025). Effects of nutrient solution and substrate on Limnocharis flava performance in hydroponic systems. Asian Journal of Agriculture, 9(2). https://doi.org/10.13057/asianjagric/g090225

References

Adhikari L, Baral R, Paudel D, Min D, Makaju SO, Poudel HP, Acharya JP, Missaoui AM. 2022. Plant stress cold stress in plants: Strategies to improve cold tolerance in forage species. Plant Stress 4: 100081. DOI: 10.1016/j.stress.2022.100081.

Azmin SNHM, Sulaiman NS, Nor MSM, Abdullah PS, Kari ZA, Pati S. 2022. A review on recent advances on natural plant pigments in foods: Functions, extraction, importance and challenges. Appl Biochem Biotechnol 194: 4655-4672. DOI: 10.1007/s12010-022-04050-z.

Chatara T, Musvosvi C, Houdegbe AC, Tesfay SZ, Sibiya J. 2023. Biochemical characterization of African spider plant (Gynandropsis gynandra (L.) Briq.) genotypes under drought and non-drought conditions. Front Plant Sci 14: 1197462. DOI: 10.3389/fpls.2023.1197462.

Currey CJ, Walters KJ, Flax NJ. 2019. Nutrient solution strength does not interact with the daily light integral to affect hydroponic cilantro, dill, and parsley growth and tissue mineral nutrient concentrations. Agronomy 9 (7): 389. DOI: 10.3390/agronomy9070389.

Ding X, Jiang Y, Zhao H, Guo D, He L, Liu F, Zhou Q, Nandwani D, Hui D, Yu J. 2018. Electrical conductivity of nutrient solution influenced photosynthesis, quality, and antioxidant enzyme activity of pakchoi (Brassica campestris L. ssp. Chinensis) in a hydroponic system. PLoS ONE 13 (8): e0202090.. DOI: 10.1371/journal.pone.0202090.

Do Carmo APM, Freitas MSM, Machado LC, Silva LdS, Petri DJC, Vimercati JC, Matos CRR, Mathias L, Viera IJC, Carvalho AJC. 2024. Electrical conductivity of nutrient solutions affects the growth, nutrient levels, and content and composition of essential oils of Acmella oleracea (L.) R. K. Jansen from southeastern Brazil. J Agric Food 15: 100968. DOI: 10.1016/j.jafr.2024.100968.

Dylag A, Smolen S, Wista-Swider A, Kowalska I, Sularz O, Krzemiriska J, Pitala J, Koronowicz A. 2023. Evaluation of the chemical composition and nutritional value of lettuce (Lactuca sativa L.) biofortified in hydroponics with iodine in the form of iodoquinolines. Front Plant Sci 14: 1288773. DOI: 10.3389/fpls.2023.1288773.

El-Hendawy S, Dewir YH, Elsayed S, Schmidhalter U, Al-Gaadi K, Tola E, Refay Y, Tahir MU, Hassan WM. 2022. Combining hyperspectral reflectance indices and multivariate analysis to estimate different units of chlorophyll content of spring wheat under salinity conditions. Plants 11 (3): 456. DOI: 10.3390/plants11030456.

Fathidarehnijeh E, Nadeem M, Cheema M, Thomas R, Krishnapillai M, Galagedara L. 2024. Current perspective on nutrient solution management strategies to improve the nutrient and water use efficiency in hydroponic systems. Can J Plant Sci 102 (2): 88-102. DOI: 10.1139/cjps-2023-0034.

Fimbres-Acedo YE, Traversari S, Cacini S, Costamagna G, Ginepro M, Massa D. 2022. Testing the effect of high pH and low nutrient concentration on four leafy vegetables in hydroponics. Agronomy 13 (1): 41. DOI: 10.3390/Agronomy13010041.

Gillespie DP, Papio G, Kubota C. 2021. High nutrient concentrations of hydroponic solution can improve growth and nutrient uptake of spinach (Spinacia oleracea L.) grown in acidic nutrient solution. HortScience 56 (6): 687–694. DOI: 10.21273/HORTSCI15777-21.

Gomez K, Gomez A. 1984. Statistical procedures for agricultural research. 2nd ed. Wiley-Interscience, New York.

Harich H, Hazra, Alfasane MA, Kauser S, Shahjadee UF, Khondker M. 2019. Biochemical composition of some selected aquatic macrophytes under ex-situ conditions. J Asiatic Soc Bangladesh Sci 44 (1): 53-60. DOI: 10.3329/jasbs.v44i1.46545.

Hosseini H, Mozafari V, Roosta HR, Shirani H, Van De Vlasakker PCH, Farhangi M. 2021. Nutrient use in vertical farming: Optimal electrical conductivity of nutrient solution for growth of lettuce and basil in hydroponic cultivation. Horticulturae 7 (9): 283. DOI: 10.3390/horticulturae7090283.

Hou W, Shen J, Xu W, Khan MR, Wang Y, Zhou X, Gao Q, Murtaza B, Zhang Z. 2021. Recommended nitrogen rates and the verification of effects based on leaf SPAD readings of rice. PeerJ 9: e12107. DOI: 10.7717/peerj.12107.

Ighalo JO, Ohoro CR, Ojukwu VE, Oniye M, Shaikh WA, Biswas JK, Seth CS, Babu G, Mohan M, Chandran SA, Rangabhashiyam S. 2025. Review biochar for ameliorating soil fertility and microbial diversity: From production to action of the black gold. iScience 28: 111524. DOI: 10.1016/j.isci.2024.111524.

Kappel N, Boros F, Ravelombola FS, Sipos L. 2021. EC sensitivity of hydroponically-grown lettuce (Lactuca sativa L.) types in terms of nitrate accumulation. Agriculture 11 (4): 315. DOI: 10.3390/agriculture11040315.

Lazarevi? B, Gunja?a J, Safner T, Vidak M, Javornik T, Carovi?-Stanko K. 2024. Multispectral and chlorophyll fluorescence imaging for detection of nutrient deficiency symptoms in common bean. J Cent Eur Agric 25 (2): 416-432. DOI: 10.5513/JCEA01/25.2.4305.

?a?ny R, Mirgos M, Przyby? JL, Nowak JS, Kunka M, Gajc-Wolska J, Kowalczyk K. 2021. Effect of re-used lignite and mineral wool growing mats on plant growth, yield and fruit quality of cucumber and physical parameters of substrates in hydroponic cultivation. Agronomy 11 (5): 998. DOI: 10.3390/agronomy11050998.

Li J, Cao X, Jia X, Liu L, Cao H, Qin W, Li M. 2021. Iron deficiency leads to chlorosis through impacting chlorophyll synthesis and nitrogen metabolism in Areca catechu L. Front Plant Sci 12: 710093. DOI: 10.3389/fpls.2021.710093.

Li J, Lu X, Ju W, Li J, Zhu S, Zhou Y. 2022. Seasonal changes of leaf chlorophyll content as a proxy of photosynthetic capacity in winter wheat and paddy rice. Ecol Ind 140: 109018. DOI: 10.1016/j.ecolind.2022.109018.

Liu X, Wang X, Zhu J, Wang X, Chen K, Yuan Y, Yang X, Mo W, Wang R, Zhang S. 2024. Strong conservatism in leaf anatomical traits and their multidimensional relationships with leaf economic traits in grasslands under different stressful environments. Ecol Process 13 (1): 71. DOI: 10.1186/s13717-024-00548-y.

Machado RMA, Alves-Pereira I, Ferreira R, Gruda NS. 2021. Coir, an alternative to peat—effects on plant growth, phytochemical accumulation, and antioxidant power of spinach. Horticulturae 7 (6): 127. DOI: 10.3390/horticulturae7060127.

Martinez-Moreno A, Carmona J, Martinez V, Garcia-Sanchez F, Mestre T, Navarro-Perez V, Cmara-Zapata J. 2024. Reducing nitrate accumulation through the management of nutrient solution in a floating system lettuce (Lactuca sativa L.). Scientia Hortic 336 (10): 113377. DOI: 10.1016/j.scienta.2024.113377.

Mthiyane P, Aycan M, Mitsui T. 2024. Integrating biofertilizers with organic fertilizers enhances photosynthetic efficiency and upregulates chlorophyll-related gene expression in rice. Sustainability 16 (21): 9297. DOI: 10.3390/su16219297.

Nasar J, Wang G, Ahmad S, Muhammad I, Zeeshan M, Gitari H, Adnan M, Fahad S, Abdelsalam NR, Ahmed GA, Hasan ME. 2022. Nitrogen fertilization coupled with iron foliar application improves the photosynthetic characteristics, photosynthetic nitrogen use efficiency, and the related enzymes of maize crops under different planting patterns. Front Plant Sci 16: 988055. DOI: 10.3389/fpls.2022.988055.

Natalli LH, Hillig E, Lombardi KC, Godinho M, Nuñez RP. 2024. Use of biochar as a component of substrates in horticulture and forestry: A review. Rev Bras Cienc Solo 48: e0240027.. DOI: 10.36783/18069657rbcs20240027.

Neocleous D, Nikolaou G, Ntatsi G, Savvas D. 2020. Impact of chelated or inorganic manganese and zinc applications in closed hydroponic bean crops on growth, yield, photosynthesis, and nutrient uptake. Agronomy 10 (6): 881. DOI: 10.3390/agronomy10060881.

Nerlich A, Dannehl D. 2021. Soilless cultivation: Dynamically changing chemical properties and physical conditions of organic substrates influence the plant phenotype of lettuce. Front Plant Sci 11: 601455. DOI: 10.3389/fpls.2020.601455.

Nkcukankcuka M, Jimoh MO, Griesel G, Laubscher CP. 2022. Growth characteristics, chlorophyll content and nutrients uptake in Tetragonia decumbens Mill. cultivated under different fertigation regimes in hydroponics. Crop Pasture Sci 73 (2): 67-76. DOI: 10.1071/CP20511.

Nurhidayati N, Ansari AS, Sholihah A, Chiangmai PN. 2022. Vermicompost and rice husk biochar interaction ameliorates nutrient uptake and yield of green lettuce under soilless culture. J Hortic Res 30 (2): 55-66. DOI: 10.2478/johr-2022-0018.

Olasehinde AA. 2025. Biodegradable growth media alternatives for sustainable hydroponic farming. Curr J Appl Sci Technol 44 (3): 147-152. DOI: 10.9734/cjast/2025/v44i34506.

Papadakis IE, Antonopoulou C, Sotiropoulos T, Chatzissavvidis C, Therios I. 2023. Effect of magnesium on mineral nutrition, chlorophyll, proline and carbohydrate concentrations of sweet orange (Citrus sinensis cv. Newhall) plants. Appl Sci 13 (14): 7995. DOI: 10.3390/app13147995.

Perkasa AY, Petropoulos S. 2020. “Genjer” Yellow velvetleaf used as indigenous vegetable in Indonesia. Anatolian J Bot 4 (1): 76-79. DOI: 10.30616/ajb.710777.

Pomoni DI, Koukou MK, Vrachopoulos MG, Vasiliadis L. 2023. A review of hydroponics and conventional agriculture based on energy and water consumption, environmental impact, and land use. Energies 16 (4): 1690. DOI: 10.3390/en16041690.

Putra SP, Carolina Y, Salsinha F. 2023. Waterlogging and salinity stress affecting growth and morphological character changes of Limnocharis flava. Biodiversitas 24 (1): 333-340. DOI: 10.13057/biodiv/d240140.

Sahin O, Gunes A, Deniz Yagc?oglu K, Kadioglu YK. 2025. Exploring the impact of rice husk biochar on oxidative mechanisms and salt stress tolerance in lettuce plants. J Plant Nutr 48 (4): 607-616. DOI: 10.1080/01904167.2024.2408419.

Saikia K, Dey S, Hazarika S, Handique G, Thakur D, Handique A. 2023. Chemical and biochemical characterization of Ipomoea aquatica: Genoprotective potential and inhibitory mechanism of its phytochemicals against ?-amylase and ?-glucosidase. Front Nutr 10: 1304903. DOI: 10.3389/fnut.2023.1304903.

Saupi N, Saidin AA, Harah M, Ya Z, Sarbini SR, Yusli NA. 2020. An ethnobotanical study of indigenous leafy vegetables among local communities in Bintulu, Sarawak, Malaysia. Borneo J Res Sci Technol 10 (2): 155-165. DOI: 10.33736/bjrst.2623.2020.

Shi P, Wang Y, Yin C, Fan K, Qian Y, Chen G. 2024. Mitigating saturation effects in rice nitrogen estimation using Dualex measurements and machine learning. Front Plant Sci 15: 1518272. DOI: 10.3389/fpls.2024.1518272.

Soufi HR, Roosta HR, Hamidpour M. 2023. The plant growth, water and electricity consumption, and nutrients uptake are influenced by different light spectra and nutrition of lettuce. Sci Rep 13: 20766. DOI: 10.1038/s41598-023-48284-1.

Sudirman S, Arianti M, Nugroho GD, Ridhowati S, Pitayati PA, Janna M. 2023. Total polyphenols and antioxidant activity of yellow velvetleaf (Limnocharis flava) extract. Intl J Secondary Metabolite 10 (4): 583-589. DOI: 10.21448/IJSM.1328150.

Susila A, Syukur M, Purnamawati H, Dharma K, Gunawan E, Evi. 2012. Koleksi dan identifikasi tanaman sayuran indigenous. Pusat Kajian Hortikultura Tropika Institut Pertanian Bogor, Bogor.

Tobassum M, Akon R, Howlader J, Malek MA. 2023. Effect of electrical conductivity on growth, yield and quality of broccoli grown in hydroponic system. Intl J Innov Res 7 (3): 43-49.

Voutsinos-Frantzis N, Ntatsi G, Karavidas I, Neofytou I, Deriziotis K, Ropokis A, Consentino BB, Sabatino L, Savvas D. 2022. Exploring the simultaneous effect of total ion concentration and K:Ca:Mg ratio of the nutrient solution on the growth and nutritional value of hydroponically grown Cichorium spinosum L. Agronomy 12 (9): 2214. DOI: 10.3390/agronomy12092214.

Xiong D, Chen J, Yu T, Gao W, Ling X, Li Y, Peng S, Huang J. 2015. SPAD-based leaf nitrogen estimation is impacted by environmental factors and crop leaf characteristics. Sci Rep 5 (1): 13389. DOI: 10.1038/srep13389.

Yang T, Samarakoon U, Altland J, Ling P. 2021. Photosynthesis, biomass production, nutritional quality, and flavor-related phytochemical properties of hydroponic-grown arugula (Eruca sativa Mill.) ‘standard’ under different electrical conductivities of nutrient solution. Agronomy 11 (7): 1340. DOI: 10.3390/agronomy11071340.

Yang T, Samarakoon U, Altland J, Ling P. 2024. Influence of electrical conductivity on plant growth, nutritional quality, and phytochemical properties of kale (Brassica napus) and collard (Brassica oleracea) grown using hydroponics. Agronomy 14 (11): 2704. DOI: 10.3390/agronomy14112704.

Yap MY, Lim MW, Brendan LKW. 2022. Influence of calcium macronutrient on tip burn occurrence in hydroponically cultivated lettuces. IOP Conf Ser: Mater Sci Eng 1257 (1): 012003. DOI: 10.1088/1757-899x/1257/1/012003.

Zhang J, Song K, Jin F, Jia F, Liang J, Wang F, Zhang J. 2024. A novel strategy of artificially regulating plant rhizosphere microbial community to promote plant tolerance to cold stress. Sci Total Environ 949: 175184. DOI: 10.1016/j.scitotenv.2024.175184.

Zhu Y, Fu Q, Zhu C, Li Y, Yuan F, Sun X, Fu J. 2024. Review on physiological and molecular mechanisms for enhancing salt tolerance in turfgrass. Grass Res 4 (1): e024. DOI: 10.48130/grares-0024-0020.

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