Rice response to cadmium bioremediation using sorghum and mycorrhiza
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Abstract. Harsono P, Hasanah NAU, Purwanto E, Samanhudi. 2025. Rice response to cadmium bioremediation using sorghum and mycorrhiza. Asian J Agric 9: 522-532. Cadmium (Cd) contamination at 2.9 mg kg-¹ in paddy soils reduces rice productivity and threatens food safety. This study aimed to evaluate the effectiveness of sorghum (Sorghum bicolor) phytoremediation combined with Arbuscular Mycorrhizal Fungi (AMF) in improving rice growth under mild Cd contamination. A factorial randomized complete block design with two cropping systems (seed vs. ratoon, where the same crop is re-grown from the stubble or roots of the first crop) and seven remediation treatments (control; three sorghum varieties (Super 1, Samurai 1, Kawali) combine with/without 10 g AMF plot-¹) was applied in 42 plots (3×4 m). Rice cv. IR 64 was transplanted 30 days after remediation. Significant interactions (p<0.05) between cropping system and remediation treatment occurred for plant height and leaf area. The Kawali + AMF treatment produced the most significant gains relative to the control: plant height +7.7%, leaf area +207%, chlorophyll +236% and total dry biomass +152%, while extending the vegetative phase by 23%. Improvements were consistent across sorghum seed and sorghum ratoon systems, indicating that remediation benefits persist beyond the first harvest. Ratoon sorghum-maintained remediation benefits, with 7-10% taller plants and 8% greater leaf area than the control, despite slightly reduced canopy size compared with seed sorghum; all sorghum treatments delayed flowering by ~11 days, indicating reduced Cd stress. High-biomass sorghum, especially Kawali, combined with AMF, enhances rice growth and physiology while stabilizing Cd, providing a scalable remediation strategy for sustainable rice production in Cd-contaminated tropical agroecosystems.
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Al-Huqail AA, Seleiman MF, Aljabri M, Ahmad A, Alotaibi M, Battaglia ML. 2024. Phytoremediation: A promising approach for re-vegetation of Cadmium-Polluted land. In: Jha AK, Kumar N (eds). Cadmium Toxicity Mitigation. Springer, Cham. DOI: 10.1007/978-3-031-47390-6_9.
Babadi M, Zalaghi R, Taghavi M. 2019. A non-toxic polymer enhances sorghum-mycorrhiza symbiosis for bioremediation of Cd. Mycorrhiza 29 (4): 375-387. DOI: 10.1007/s00572-019-00902-5.
Bai Z, Li D, Zhu L, Tang X, Wang Y, Mao R, Wu J. 2021. Nitrate increases cadmium accumulation in sweet sorghum for improving phytoextraction efficiency rather than ammonium. Front Plant Sci 12: 643116. DOI: 10.3389/fpls.2021.643116.
Ballabio C, Jones A, Panagos P. 2023. Cadmium in topsoils of the European Union – An analysis based on LUCAS topsoil database. Sci Total Environ 912: 168710. DOI: 10.1016/j.scitotenv.2023.168710.
Bao X, Liu J, Qiu G, Chen X, Zhang J, Wang H, Zhang Q, Guo B. 2025. The effect of Rhizophagus intraradices on cadmium uptake and OsNRAMP5 gene expression in rice. Intl J Mol Sci 26 (4): 1464. DOI: 10.3390/ijms26041464.
Behera PP, Saharia N, Borah N, Devi SH, Sarma RN. 2022. Sorghum physiology and adaptation to abiotic stresses. Intl J Environ Clim Chang 12 (10): 1005-1022. DOI: 10.9734/ijecc/2022/v12i1030891.
Bhantana P, Rana MS, Sun X, Moussa MG, Saleem MH, Syaifudin M, Shah A, Poudel A, Pun AB, Bhat MA, Mandal, DL, Shah S, Zhihao D, Tan Q, Hu C. 2021. Arbuscular mycorrhizal fungi and its major role in plant growth, zinc nutrition, phosphorous regulation and phytoremediation. Symbiosis 84: 19-37. DOI: 10.1007/s13199-021-00756-6.
Cai Y. 2025. The impact of different fertilization methods on soil heavy metal pollution and restoration methods. Theor Nat Sci 75: 136-145. DOI: 10.54254/2753-8818/2024.la19383.
Chauhan NS, Mahawar SK, Jain D, Udpadhay SK, Mohanty SR, Singh AW, Maharjan E. 2022. Boosting sustainable agriculture by arbuscular mycorrhiza under stress condition: Mechanism and future prospective. BioMed Res Intl 2022: 1-28. DOI: 10.1155/2022/5275449.
Chen C, Wang X, Wang J. 2019. Phytoremediation of cadmium-contaminated soil by Sorghum bicolor and the variation of microbial community. Chemosphere 235: 985-994. DOI: 10.1016/j.chemosphere.2019.07.028.
Chen J, Guo J, Li Z, Liang X, You Y, Li M, He Y, Zhan F. 2022. Effects of an arbuscular mycorrhizal fungus on the growth of and cadmium uptake in maize grown on polluted wasteland, farmland and slopeland soils in a lead-zinc mining area. Toxics 10 (7): 359. DOI: 10.3390/toxics10070359.
Chen R, Chen X, Xu Y, Ali I, Zhu W, Liu J, Wang Q, Huang W, Dai, X. 2025. Enhancing remediation efficiency of cadmium-contaminated soil: integrating forage-microorganism systems with agronomic strategies. Environ Geochem Health 47: 67. DOI: 10.1007/s10653-025-02383-2.
Cheng P, Zhang S, Wang Q, Feng X, Zhang S, Sun Y, Wang F. 2021. Contribution of nano-zero-valent iron and arbuscular mycorrhizal fungi to phytoremediation of heavy metal-contaminated soil. Nanomaterials 11 (5): 1264. DOI: 10.3390/nano11051264.
da Silva JB, Rosa GB, Sganzerla WG, Ferrareze JP, Simioni FJ, Campos M. 2023. Strategies and prospects in the recovery of contaminated soils by phytoremediation: An updated overview. Commun Plant Sci 13: 1-12. DOI: 10.26814/cps2023001.
Dai J, Chen Y, Yang W, Liu Z, Deng X, Yang Y, Zeng Q. 2024. Field evaluation of oil crop rotations for cadmium remediation and safe vegetable oil production across five sites with varying contamination levels. Ecotoxicol Environ Saf 284: 116897. DOI: 10.1016/j.ecoenv.2024.116897.
de Resende ÁV, Giehl J, de Paula Simão E, de Abreu SC, Galvão JCC, Borghi E, Gontijo Neto MM. 2022. Nutrient removal by off-season grain sorghum as affected by intercropping with ruzigrass and fertilization levels in the Brazilian cerrado. Revista Brasileira de Milho e Sorgo 21: e1282. DOI: 10.18512/10.18512/rbms2022v21e1282.
Dharma K, Suryanti S, Widiastuti A. 2024. Hormesis in pathogenic and biocontrol fungi: From inhibition to stimulation. Caraka Tani: J Sustain Agric 39 (2): 281-296. DOI: 10.20961/carakatani.v39i2.83012.
Eid EM, Shaltout KH, Alamri SM, Alrumman SA, Hussain AA, Sewelam N, Ragab GA. 2021. Monitored sewage sludge application improves soil quality, enhances plant growth, and provides evidence for metal remediation by Sorghum bicolor L. J Soil Sci Plant Nutr 21: 2325-2338. DOI: 10.1007/s42729-021-00524-x.
Etesami H, Jeong BR, Glick BR. 2021. Contribution of arbuscular mycorrhizal fungi, Phosphate–Solubilizing bacteria, and silicon to P uptake by plant. Front Plant Sci 12: 699618. DOI: 10.3389/fpls.2021.699618.
Farooq M, Nawaz A, Nadeem F, Bajwa HR, Salim MA, Rehman A, Ullah A. 2022. Brown manuring and sorghum allelopathy affects weeds, soil health, and paddy yield. Agron J 114: 3040-3051. DOI: 10.1002/agj2.21159.
Gao Y, Liu R, Chen Y. 2024. The role of AM fungi in the alleviation of cadmium stress in crops. In: Parihar M, Rakshit A, Adholeya A, Chen Y (eds). Arbuscular Mycorrhizal Fungi in Sustainable Agriculture: Nutrient and Crop Management. Springer, Singapore. DOI: 10.1007/978-981-97-0300-5_13.
Ghimire OP, Lazo A, Parajuli B, Nepal J. 2024. Fostering Microbial Activity and Diversity in Agricultural Systems. CSA News 69 (8): 30-35. DOI: 10.1002/csan.21344.
Grant CA. 2015. Influence of phosphate fertilizer on cadmium in agricultural soils and crops. In: Selim HM (eds). Phosphate in Soils. CRC Press, Boca Raton. DOI: 10.1201/9781351228909-9.
Guigard L, Jobert L, Busset N, Moulin L, Czernic P. 2023. Symbiotic compatibility between rice cultivars and arbuscular mycorrhizal fungi genotypes affects rice growth and mycorrhiza-induced resistance. Front Plant Sci 14: 1278990. DOI: 10.3389/fpls.2023.1278990.
Gutiérrez E, Chavez E, Gamage K, Argüello D, Galkaduwa MB, Hettiarachchi GM. 2022. Cadmium fractionation in soils affected by organic matter application: Transfer of cadmium to cacao (Theobroma cacao L.) tissues. Front Environ Sci 10: 954521. DOI: 10.3389/fenvs.2022.954521.
Hamid Y, Tang L, Sohail MI, Cao X, Hussain B, Aziz MZ, Usman M, He Z, Yang X. 2019. An explanation of soil amendments to reduce cadmium phytoavailability and transfer to food chain. Sci Total Environ 660: 80-96. DOI: 10.1016/j.scitotenv.2018.12.419.
Han B. 2019. Study on soil remediation technology of cadmium contaminated site. Open J Appl Sci 9 (3): 115-120. DOI: 10.4236/ojapps.2019.93010.
Hasanah NAU, Purwanto E, Harsono P, Samanhudi S, Sakya AT. 2023. Morphological responses of six sorghum varieties on cadmium-contaminated soil. Biodiversitas 24 (7): 3903-3915. DOI: 10.13057/biodiv/d240729.
Hassan MJ, Raza MA, Rehman SU, Ansar M, Gitari H, Khan I, Wajid M, Ahmed M, Shah GA, Peng Y, Li Z. 2020. Effect of cadmium toxicity on growth, oxidative damage, antioxidant defense system and cadmium accumulation in two sorghum cultivars. Plants 9 (11): 1575. DOI: 10.3390/plants9111575.
Hou D, Jia X, Wang L, McGrath SP, Zhu Y, Hu Q, Zhao F, Bank MS, O’Connor D, Nriagu J. 2025. Global soil pollution by toxic metals threatens agriculture and human health. Science 388 (6744): 316-321. DOI: 10.1126/science.adr5214.
Huang M, Xiao Z, Fang S, Zhang H, Liu L, Cao F, Chen J. 2024. Achieving super high yield in rice by simultaneously increasing panicle number and grain weight via improving pre-heading biomass production. Exp Agric 60: e20. DOI: 10.1017/s0014479724000140.
Huang X, Zhao B, Wu Y, Tan M, Shen L, Feng G, Yang X, Chen S, Xiong Y, Zhang E, Zhou H. 2022. The lead and cadmium content in rice and risk to human health in China: A systematic review and meta-analysis. PLoS ONE 17 (12): e0278686. DOI: 10.1371/journal.pone.0278686.
Jiang P, Zhang L, Chen C, Zhou X, Liu M, Xiong H, Guo X, Zhu Y, Xu F. 2023. Progress and challenges of rice ratooning technology in Sichuan Province, China. Crop Environ 2 (3): 111-120. DOI: 10.1016/j.crope.2023.04.006.
Karimah R, Purwanto B, Hanudin E, Utami S, Maimunah M. 2024. Effect of different types of biochar applications and phosphate fertilizer on the quality and yield of edamame soybeans on andisols. Caraka Tani J Sustain Agric 39 (1): 117-139. DOI: 10.20961/carakatani.v39i1.80217.
Karimi K, Malakouti M, Davari D, Asbaghian A. 2022. Effect of different sources of phosphate fertilizers on cadmium accumulation in potato tubers (Solanum tuberosum L.) in the calcareous soils of Iran. Ann Agric Crop Sci 6 (7): 1101. DOI: 10.26420/annagriccropsci.2021.1101.
Kaur H, Tashima N, Singh S, Kumar P. 2022. Reconditioning of plant metabolism by arbuscular mycorrhizal networks in cadmium contaminated soils: Recent perspectives. Microbiol Res 268: 127293. DOI: 10.1016/j.micres.2022.127293.
Koushal S, Kanagalabavi AC, Kumar A, Arya DS, Nehul JN, Panigrahi CK, Haloi D, Chauhan N, Muhilan G. 2025. Bioremediation of soil pollution: an effective approach for sustainable agriculture. Intl J Plant Soil Sci 37 (1): 400-410. DOI: 10.9734/ijpss/2025/v37i15282.
Li C, Yang G, Liu Z, Cai J. 2022. Overview of phytoremediation technology for heavy metal contaminated soil. E3S Web Conf 350: 01006. DOI: 10.1051/e3sconf/202235001006.
Lian M, Ma Y, Li J, Sun J, Zeng X. 2022. Influence of pH on the particulate-bound Cd speciation and uptake by plants. Pol J Environ Stud 31 (6): 5511-5517. DOI: 10.15244/pjoes/152224.
Liu G, Dai Z, Tang C, Xu J. 2022. The immobilization, plant uptake and translocation of cadmium in a soil-pakchoi (Brassica chinensis L.) system amended with various sugarcane bagasse-based materials. Environ Pollut 311: 119946. DOI: 10.1016/j.envpol.2022.119946.
Liu M, Luo X, Gu J, Yi X, Zhou H, Zeng P, Liao B. 2024. Cadmium phytoremediation effect of sweet sorghum assisted with citric acid on typical parent soil in southern China. Huan Jing Ke Xue 45 (5): 3016-3026. DOI: 10.13227/j.hjkx.202306027.
Liu Y, Liu K, Li Y, Yang W, Wu F, Zhu P, Zhang J, Chen L, Gao S, Zhang L. 2015. Cadmium contamination of soil and crops is affected by intercropping and rotation systems in the lower reaches of the Minjiang River in south-western China. Environ Geochem Health 38: 811-820. DOI: 10.1007/s10653-015-9762-4.
Mindari W, Chakim M, Widjajani B, Sasongko P, Aditya H, Pazi A, Gandaseca S. 2025. The optimization of biosilica and humic acid to increase soil nutrient availability and nutrient uptake in rice plant in sandy soil. Caraka Tani: J Sustain Agric 40 (1): 18-33. DOI: 10.20961/carakatani.v40i1.89018.
Miranda R, Boechat CL, Bomfim MR, Santos JAG, Coelho DG, Assunção SJR, Cardoso KM, Cardoso EB. 2022. 3 - Phytoremediation: A sustainable green approach for environmental cleanup. In: Kumar V, Shah MP, Shahi SK (eds). Phytoremediation Technology for the Removal of Heavy Metals and Other Contaminants from Soil and Water. Elsevier, Amsterdam. DOI: 10.1016/b978-0-323-85763-5.00017-9.
Murtaza G, Usman M, Iqbal R, Rahman MHU, Ali I, Arslan M. 2022. Cadmium concertation and health risk assessment of ground-water in District Khanewal (South Punjab) Pakistan. Res Sq (Preprint) 2022: 1-21. DOI: 10.21203/rs.3.rs-2338449/v1.
Musa IO, Ijah UJJ, Abioye OP, Abdulsalam M, Pal SK, Abamhekhelu IA, Maude AM, Yusuf OO, Akande SA. 2024. Phytoremediation of heavy metal-contaminated soil. In: Rathod SV (eds). Eco-Restoration of Polluted Environment: A Biological Perspective. CRC Press, Boca Racon. DOI: 0.1201/9781003423393-2.
Pratama I, Suryantini A, Perwitasari H. 2024. Sustainability of the different rice cultivation practices in Yogyakarta, Indonesia. Caraka Tani: J Sustain Agric 39 (2): 321-342. DOI: 10.20961/carakatani.v39i2.85817.
Radha R, Kumutha K, Marimuthu P. 2014. Assessment of cadmium contamination of soils in sewage disposal areasof Coimbatore District, Tamil Nadu, India. Curr World Environ 9: 379-386. DOI: 10.12944/cwe.9.2.18.
Rajput A, Ali Panhwar Q, Babar H. 2024. Role of leguminous crops by enhancing soil fertility and plant nutrition. In: Jimenez-Lopez JC, Escudero-Feliu J (eds). Legume Crops for Food Security - Cultivation and Benefits. IntechOpen, London. DOI: 10.5772/intechopen.1006827.
Resmi G, Thampi SG, Chandrakaran S. 2023. Heavy metal removal from contaminated soil using soil washing techniques. Nat Environ Pollut Technol 22: 129-138. DOI: 10.46488/nept.2023.v22i01.011.
Sagimbayeva AM. 2023. Selection of highly productive genotypes of sugar sorghum (Sorghum bicolor l) for further use in phytoremediation of soils from heavy metals. In: Prasad MNV (eds). Biosafety and Biotechnology. Elsevier, Amsterdam. DOI: 10.58318/2957-5702-2022-11-32-38.
Singh BR, Kc D, Ra A. 2017. Long-term effect of phosphate fertilization on cadmium uptake by oat and its accumulation in soil. J Environ Anal Toxicol 7: 6. DOI: 10.4172/2161-0525.1000516.
Soma F, Kitomi Y, Kawakatsu T, Uga Y. 2023. Life-cycle multiomics of rice shoots reveals growth stage–specific effects of drought stress and time–lag drought responses. Plant Cell Physiol 65 (1): 156-168. DOI: 10.1093/pcp/pcad135.
Suciu NA, De Vivo R, Rizzati N, Capri E. 2022. Cd content in phosphate fertilizer: Which potential risk for the environment and human health? Curr Opin Environ Sci Health 30: 100392. DOI: 10.1016/j.coesh.2022.100392.
Tang X, Ni Y. 2021. Review of remediation technologies for cadmium in soil. E3S Web Conf 233: 01037. DOI: 10.1051/e3sconf/202123301037.
Ullah A, Gao D, Wu F. 2024. Common mycorrhizal network: the predominant socialist and capitalist responses of possible plant–plant and plant–microbe interactions for sustainable agriculture. Front Microbiol 15: 1183024. DOI: 10.3389/fmicb.2024.1183024.
Wahab A, Muhammad M, Munir A, Abdi G, Zaman W, Ayaz A, Khizar C, Reddy SPP. 2023. Role of arbuscular mycorrhizal fungi in regulating growth, enhancing productivity, and potentially influencing ecosystems under abiotic and biotic stresses. Plants 12 (17): 3102. DOI: 10.3390/plants12173102.
Wang G, Zhang Q, Du W, Lin R, Li J, Ai F, Yin Y, Ji R, Wang X, Guo H. 2021. In-situ immobilization of cadmium-polluted upland soil: A ten-year field study. Ecotoxicol Environ Saf 207: 111275. DOI: 10.1016/j.ecoenv.2020.111275.
Wang J, Delavar MA. 2023. Techno-economic analysis of phytoremediation: A strategic rethinking. Sci Total Environ 902: 165949. DOI: 10.1016/j.scitotenv.2023.165949.
Wang L, Sun X, Li S, Zhang T, Zhang W. 2022. Sweet sorghum combined with amendments reduces cadmium availability in contaminated soils. Environ Sci Pollut Res 29: 18341-18350. DOI: 10.1007/s11356-021-17092-9
Wang S, Li B, Zhu H, Liao W, Wu C, Zhang Q, Tang K, Cui H. 2023. Energy sorghum Removal of soil cadmium in Chinese subtropical farmland: effects of variety and cropping system. Agronomy 13 (10): 2487. DOI: 10.3390/agronomy13102487.
Wintermans JFGM, De Mots A. 1965. Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanol. Biochim Biophys Acta 109 (2): 448-453. DOI: 10.1016/0926-6585(65)90170-6.
Xing L, Li W, Zhai Y, Hu X, Guo S. 2022. Arbuscular mycorrhizal fungi promote early flowering and prolong flowering in Antirrhinum majus L. by regulating endogenous hormone balance under field-planting conditions. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 50 (1): 12503. DOI: 10.15835/nbha50112503.
Xu Y, Lambers H, Feng J, Tu Y, Peng Z, Huang J. 2024. The role of arbuscular mycorrhizal fungi in micronutrient homeostasis and cadmium uptake and transfer in rice under different flooding intensities. Ecotoxicol Environ Saf 284: 116978. DOI: 10.1016/j.ecoenv.2024.116978.
Yang Y, Li Y, Li X, Yan J, Wu L, Tang Z, He Y, Zhan F. 2024. Mycorrhizal extraradical mycelium can reduce cadmium uptake by maize and cadmium leaching from contaminated soil: Based on an in-growth core experiment. Front Microbiol 15: 1507798. DOI: 10.3389/fmicb.2024.1507798.
Zhang F, Wang P, Li L. 2020. Arbuscular mycorrhizal fungi reduce cadmium uptake and translocation in plants: A meta-analysis. Environmental Pollution 263: 114608. DOI: 10.1016/j.envpol.2020.114608
Zhang H, Xu N, Li X, Long J, Sui X, Wu Y, Li J, Wang J, Zhong H, Sun GY. 2018. Arbuscular mycorrhizal fungi (Glomus mosseae) improves growth, photosynthesis and protects photosystem II in leaves of Lolium perenne L. in cadmium contaminated soil. Front Plant Sci 9: 1156. DOI: 10.3389/fpls.2018.01156.
Zhang H, Xu Y, Kanyerere T, Wang Y, Sun M. 2022. Washing reagents for remediating heavy-metal-contaminated soil: A review. Front Earth Sci 10: 901570. DOI: 10.3389/feart.2022.901570.
Zhang X, Wang ZL, Lu Y, Wei J, Qi S, Wu B, Cheng S. 2024. Sustainable remediation of soil and water utilizing arbuscular mycorrhizal fungi: A review. Microorganisms 12 (7): 1255. DOI: 10.3390/microorganisms12071255.
Zhao S, Yan L, Kamran M, Liu S, Riaz M. 2024. Arbuscular mycorrhizal fungi-assisted phytoremediation: A promising strategy for cadmium-contaminated soils. Plants 13 (23): 3289. DOI: 10.3390/plants13233289.
Zhou W, He M, Tian X, Guan Q, Yu X, Bu Q, Li X. 2024. Genome editing of RICE FLOWERING LOCUS T 1 promoter delayed flowering in rice. Plant Growth Regul 103: 503-507. DOI: 10.1007/s10725-024-01118-0.
Zhou Y, Huang J, Li Z, Wu Y, Zhang J, Zhang Y. 2022. Yield and quality in main and ratoon crops of grain sorghum under different nitrogen rates and planting densities. Front Plant Sci 12: 778663. DOI: 10.3389/fpls.2021.778663.