Genetic transformation of OsMYB6 and OsMYB7 transcription factor into Nipponbare rice cultivar for lignin content manipulation

Abstract

Windiastri VE, Pantouw CF, Astuti D, Widyajayantie D, Estiati A, Nugroho S. 2018. Genetic transformation of OsMYB6 and
OsMYB7 transcription factor into Nipponbare rice cultivar for lignin content manipulation. Pros Sem Nas Masy Biodiv Indon 4: 220-
224. Lignin is a raw natural material for fuel and chemical industry. In near future, it is predicted that lignin demands for those
industries will increase significantly. In the current situation, hard woody plants, usually from the forest and plantation, are the main
resource of lignin production. It is because woody plants have higher content of lignin than non woody plants, i.e. sorghum, rice and
alang-alang. Enhanced lignin content manipulation in the non woody plants, grass plants for an example, could be a solution to get other
renewable sources, than woody plants, for lignin production. Biosynthesis of lignin are regulated by many genes and transcription
factors, among of them identified from rice, i.e., OsMYB6 and OsMYB7 and also involved in secondary cell wall formation. To
manipulate lignin content in the rice plants, we transformed OsMYB6, OsMYB7 transcription factors and pC1305 empty plasmid as
control into Nipponbare rice cultivar mediated by Agrobacterium. Transformation efficiencies for OsMYB6, OsMYB7 and pC1305
control are 5%, 11% and 4%, respectively. The result of marker gene (hptII) amplification from the total genome DNA of the transformant
plants showed that OsMYB6 and OsMYB7 transcription factors were successfully transformed into Nipponbare rice cultivar.