Polymorphism of growth hormone receptor (GHR) gene in Pasundan cattle

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WIDYA PINTAKA BAYU PUTRA
PASKAH PARTOGI AGUNG
SAIFUL ANWAR
SYAHRUDDIN SAID
ALFANDY HERMANSYAH

Abstract

Putra WPB, Agung PP, Anwar S, Said S, Hermansyah A. 2017. Polymorphism of growth hormone receptor (GHR) gene in Pasundan cattle. Pros Sem Nas Masy Biodiv Indon 3: 299-303. Identification of genetic polymorphism which controlling to the growth traits could be used as molecular selection for livestock. The objective of this study was to identify the genetic polymorphism of GHR in Pasundan cattle. Total of 119 DNA samples of Pasundan cattle that kept at BSD-BC of Ciamis, West Java was used in this study. The method used in the present study was PCR-RFLP with AluI (AG*CT) restriction enzyme. The result of this study showed that the GHR gene (298 bp) of Pasundan cattle were polymorphic with three genotype type of AA (0.412); AG (0.429) and GG (0.160). The Allelic frequencies of A and G were 0.626 and 0.374 respectively. The estimation of allelic effective numbers (Ne) was 1.873 and no fixation effect indicated (Fis<1.00). The Chi-square (χ2) test showed that the genotypic frequencies of GHR gene in the population tested was in the Hardy-Weinberg equilibrium (HWE). The observed of heterozygosity (Ho) values in this study were higher than expected of heterozygosity (He) value. The polymorphism information content (PIC) value was 0.358 (moderate). The conclusion of this study conducted that the GHR gene of Pasundan cattle was polymorphic and might be used as molecular selection through intensive study. Additional sample from another Pasundan’s population places are needed to provide accurate information about the polymorphism of GHR gene in Pasundan cattle.

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