Potency test of ointment from ethanol extract of djengkols fruit peel to accelerate wound closure in diabet mice models

Abstract

Malini DM, Madihah, Kamilawati F. 2017. Potency test of ointment from ethanol extract of djengkols fruit peel to accelerate wound closure in diabet mice models. Pros Sem Nas Masy Biodiv Indon 3: 205-210. Wounds caused by complications of diabetes cause tissue damage and difficult to cure. One of the traditional drugs used by local communities to treat diabetic wounds is fruit peel of djengkol (Archidendron pauciflorum (Benth.) I.C. Nielsen). This study aims to obtain optimum concentrations of ointment from ethanol extract of djengkols fruit peel which accelerates wound closure at the skin of mice (Mus musculus Linnaeus, 1758) induced by streptozotocin. This study uses a completely randomized design with six treatments and four replications. Diabetic mouse models were inducing by intraperitoneal injection of streptozotocin dose of 180 mg/kg body weight. A total of 20 models of diabetic mice were injured ± 1.5 cm using sterile scissors in the dorsolateral region, then divided into five treatment namely only ointment base (KP), ointments Betadine® (PB),ointment from ethanol extract of djengkols fruit peel at concentration of 5 % (P1), 10% (P2) or 15% (P3). KN treatment performed on four non-diabetic rats were only given ointment base. The ointment applied twice a day for 14 days. The results showed that the treatment of the ointment from ethanol extract of djengkols fruit peel accelerates skin wound closure in diabetic mice models. In mice treated with the ointment at a concentration of 10% (P2) showed the shortest cuts on the morphological observation at day 3, 7 and 14 that were significantly different with KP and PB (p<0.05), but comparable to the KN treatment. Based on the results of this study, the applying of ointment from ethanol extract of djengkols fruit peel at a concentration of 10% was optimum to accelerate skin wound closure in diabetic mouse models.