Maximizing Pleurotus ostreatus yield with sustainable agricultural waste substrates in Arakan, Cotabato, Philippines

Main Article Content

MAYBELYN LUPAS PEDROSO
ANNIE LEE CAYABAS ALAGOS
FRANCISCO JR. OLERMO ESGRINA
JOEFREY FEROLIN HABIBUN
JOSIE NACIONALES MAUN
RICHIE MAE MONDIDO RODRIGUEZ

Abstract

Abstract. Pedroso ML, Alagos ALC, Esgrina FJO, Habibun JF, Maun JN, Rodriguez RMM. 2025. Maximizing Pleurotus ostreatus yield with sustainable agricultural waste substrates in Arakan, Cotabato, Philippines. Nusantara Bioscience 17: 57-67. Mushroom production supports a circular economy by transforming agricultural waste into valuable food resources while enhancing local agricultural economies. This study evaluates the potential of locally available agricultural waste substrates-chopped rice straw, aged rice hull, rough sawdust, and fine sawdust-for optimizing the production of oyster mushrooms (Pleurotus ostreatus (Jacq.) P.Kumm. in Arakan, Cotabato, Philippines. The effects of these substrates on key growth parameters, including mycelial growth, fruiting time, yield, and economic returns, were assessed. A Completely Randomized Design with five replicates per treatment was employed, using a substrate mixture of 89% agricultural waste, 10% molasses, and 1% lime. Statistical analysis revealed that fine sawdust facilitated faster mycelial growth and reduced fruiting time, whereas rough sawdust produced higher yields and larger mushrooms. Economic analysis indicated that rough sawdust yielded the highest return on investment at 190.21%, making it the most cost-effective substrate. These findings demonstrate the potential of agricultural waste as a sustainable substrate for oyster mushroom cultivation, promoting environmental sustainability and profitability for small-scale farmers. The study emphasizes the critical role of substrate selection in optimizing mushroom yield and provides practical insights for commercial mushroom production.

Article Details

Section

Articles

How to Cite

PEDROSO, M.L. (2025) “Maximizing Pleurotus ostreatus yield with sustainable agricultural waste substrates in Arakan, Cotabato, Philippines”, Nusantara Bioscience, 17(1). doi:10.13057/nusbiosci/n170107.

References

Adams A, Seecharran D, Ansari A. 2022. Growth of oyster mushroom using sawdust and agriculture waste as substrates. Mushroom Research, 31(1), 73-80. https://epubs.icar.org.in/index._php/MR/article/view/122341

Aditya B, Neeraj N, Jarial RS, Jarial K, Bhatia JN. 2024. Comprehensive review on oyster mushroom species (Agaricomycetes): Morphology, nutrition, cultivation and future aspects. Heliyon, 10(5): e26539. DOI: 10.1016/j.heliyon.2024.e26539

Akter M, Halawani RF, Aloufi FA, Taleb MA, Akter S, Mahmood S. 2022. Utilization of agro-industrial wastes for the production of quality oyster mushrooms. Sustainability, 14(2): 994. https://doi.org/10.3390/su14020994

Alvarez LV, Bautista AB. 2021. Growth and yield performance of Pleurotus on selected lignocellulosic wastes in the vicinity of PUP main campus, Philippines. Indian Journal of Science and Technology 14(3): 259-269. DOI: 10.17485/IJST/v14i3.389

Argaw B, Tesfay T, Godifey T, Asres N. 2023. Growth and yield performance of oyster mushroom (P. ostreatus (Jacq.: Fr.) Kummer) using waste leaves and sawdust. International Journal of Agronomy, 8013491. DOI: 10.1155/2023/8013491

Bebarta PP, Kumar M, Das S, Pandey A, Gupta SK, Choudhary A, Geetika. 2022. An overview on oyster mushroom: Improving human health and quality of life. The Pharma Innovation Journal, 11(5): 1189-1192. https://www.thepharmajournal.com/archives/2022/vol11issue5S/PartP/S-11-5-76-488.pdf

Besufekad Y, Mekonnen A, Girma B, Daniel R, Tassema G, Melkamu J, Asefa M, Fikiru T, Dendoba L. 2019. Selection of appropriate substrate for production of oyster mushroom (Pleurotus ostreatus). Journal of Yeast and Fungal Research, 11(1): 15-25. DOI: 10.5897/JYFR2019.0187

Bhattacharjya DK, Paul RK, Miah MN, Ahmed KU. 2014. Effect of different saw dust substrates on the growth and yield of oyster mushroom (Pleurotus ostreatus). IOSR Journal of Agriculture and Veterinary Science, 7(2): 38-46. DOI: 10.9790/2380-07233846

Botchkarev A, Andru P. 2011. A return of investment as a metric for eval__uating information systems: Taxonomy and application. Interdisciplinary Journal of Information, Knowledge, and Management, 6: 245-269. DOI: 10.28945/1535

Buah J, Puije G, Bediako E, Abole E, Showemimo F. 2010. The growth and yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates. Biotechnology 9: 338-342. DOI: 10.3923/biotech.2010.338.342.

Castellanos-Reyes K, Villalobos-Carvajal R, Beldarrain-Iznaga T. 2021. Fresh mushroom preservation techniques. Foods, 10(9): 2126. DOI: 10.3390/foods10092126

Chandraprakash S, Suresh S, Muthuraja B, Kasinathan S, Partheeban C. 2022. Performance of various substrates on oyster (Pleurotus florida) cultivation. The Pharma Innovation Journal, 11(8): 424-426. https://www.thepharmajournal.com/archives/2022/vol11issue8/PartE/11-7-538-542.pdf

Chang, H. Y., Jeon, S. W., Cosadio, A., Icalina, C., Panganiban, Rodel, Quirino, R., & Song, Y. 2014. Status and prospect of mushroom industry in the Philippines. JPAIR Multidisciplinary Research, 16(1): 1-16. DOI: 10.7719/jpair.v16i1.268

Chauhan A, Negi S, Kaur R, Chauhan M, Dhanai R, Negi PS. 2024. Cultivation of oyster mushroom could be a viable option for doubling the farmer’s income - an overview. International Journal of Economic Plants, 11(Nov, 4): 387–393. DOI: 10.23910/2/2024.5725a

Dawadi E, Magar PB, Bhandari S, Subedi S, Shrestha S, Shrestha J. 2022. Nutritional and post-harvest quality preservation of mushrooms: A review. Heliyon, 8(12): e12093. DOI: 10.1016/j.heliyon.2022.e12093

Dey RC, Nasiruddin KM, Haque MS, Al Munsur MA Z. 2008. Production of oyster mushroom on different substrates using cylindrical block system. Progressive Agriculture 19(1): 7-12. DOI: 10.3329/pa.v19i1.16983.

Dubey D, Dhakal B, Dhami K, Sapkota P, Rana M, Poudel NS, Aryal L. 2019. Comparative study on effect of different substrates on yield performance of oyster mushroom. Global Journal of Biology, Agriculture and Health Sciences, 1-7. https://www.walshmedicalmedia.com/open-access/comparative.pdf

Dulay RM, Cabrera EC, Kalaw SP, Reyes RG. 2021. Optimization of culture conditions for mycelial growth and fruiting body production of naturally-occurring Philippine mushroom Lentinus swartzii Berk. Journal of Applied Biology & Biotechnology, 9(3): 17-25. DOI: 10.7324/JABB.2021.9303

Dung N, Tuyen D, Quang P. 2012. Morphological and genetic characteristics of oyster mushrooms and conditions effecting on its spawn growing. International Food Research Journal, 19(1): 347-352. http://www.ifrj.upm.edu.my/19%20(01)%202011/.pdf

Getachew A, Keneni A, Chawaka M. 2019. Production of oyster mushroom (Pleurotus ostreatus) on substrate composed from wheat straw, waste paper and cotton seed waste. International Journal of Microbiology and Biotechnology, 4(2): 38-44. DOI: 10.11648/j.ijmb.20190402.12

Grimm D, Sonntag E, Rahmann G. 2024. Oyster mushroom cultivation on cereal and legume straw of poor feed quality. Studies in Fungi, 9: e010. DOI: 10.48130/sif-0024-0010

Hoa H, Wang CL, Wang CH. 2018. The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43(4): 423-434, DOI: 10.5941/MYCO.2015.43.4.423

Hossain MA, Khatun MZ, Rahman ABMM, Rahman SMM, Paul SK, Miah MAS. 2005. Sugarcane bagasse – an ideal substrate for oyster mushroom cultivation. Bangladesh Journal for Sugarcane, 24(27): 137-143. https://www.researchgate.net/publication/385284329_Sugarcane_Bagasse-An_Ideal_Substrate_for_Oyster_Mushroom_Cultivation

Hultberg M, Asp H, Bergstrand KJ, Golovko O. 2023. Production of oyster mushroom (Pleurotus ostreatus) on sawdust supplemented with anaerobic digestate. Waste Management. 155(1): 1-7. DOI: 10.1016/j.wasman.2022.10.025

Hyde KD, Xu J, Rapior S. 2019. The amazing potential of fungi: 50 ways we can exploit fungi industrially. Fungal Diversity, 97: 1-136. DOI: 10.1007/s13225-019-00430-9.

Islam MZ, Rahman MH, Hafiz F. 2009. Cultivation of oyster mushroom (Pleurotus flabellatus) on different substrates. International Journal of Sustainable Crop Production, 4(1): 45-48. https://ggfjournals.com/assets/uploads/45-481.pdf

Jayaraman S, Yadav B, Dalal RC, Naorem A, Sinha NK, Rao CS, Dang YP, Patra AK, Datta SP, Rao AS. 2024. Mushroom farming: A review focusing on soil health, nutritional security and environmental sustainability. Farming System, 2(3): 100098. DOI: 10.1016/j.farsys.2024.100098

Khan A, Murad W, Salahudin A, Ali S, Shah SS, Halim SA, Khalid A, Kashtoh H, Khan A, Al-Harrasi A. 2024. Contribution of mushroom farming to mitigating food scarcity: Current status, challenges and potential future prospects in Pakistan. Heliyon, 10(23): e40362. DOI: 10.1016/j.heliyon.2024.e40362

Khan NA, Ajmal M, Ul Haq MI, Javed N, Ali MA, Binyamin R, Khan SA. 2012. Impact of sawdust using various woods for effective cultivation of oyster mushroom. Pakistan Journal of Botany, 44(1): 399-402. https://www.researchgate.net/publication/339352183_IMPACT_OF_SAWDUST_USING_VARIOUS_WOODS_FOR_EFFECTIVE_CULTIVATION_OF_OYSTER_MUSHROOM

Lavenia J, Prasetyaningsih A, Ariestanti CA. 2021. Utilization of used cardboard and vegetable waste as growth media for white oyster mushroom (Pleurotus ostreatus). Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati, 6(2): 129-137. DOI: 10.24002/biota.v6i2.4278

Marshall E. Nair NG. 2009. Make money by growing mushrooms. Food and Agriculture Organization Livelihood Diversification Booklet No. 7. Rome: Rural Infrastructure and Agro-Industries Division Food and Agriculture Organization of the United Nations. https://www.fao.org/4/i0522e/i0522e00.htm

Muhammad S, Muhammad A, Sajid A, Hasan S, Rizwan L, Muhammad S. 2014. Growth and yield performance of oyster mushroom on different substrates. Mycopath, 12(1): 9-15. https://www.academia.edu/9967740/Growth_and_yield_substrates

Nithyatharani R, Kavitha US. 2018. Cultivation of oyster mushroom using different substrates. International Journal of Creative Research Thoughts, 6(1): 332-337. https://ijcrt.org/papers/IJCRT1705051.pdf

Orngu OA, Mbaeyi-Nwaoha IE, Unagwu BO, Etim VE. 2021. Oyster mushroom (Pleurotus ostreatus) cultivation using sawdust and different organic manures. Asian Food Science Journal, 20(5): 67-74. DOI: 10.9734/afsj/2021/v20i530299.

Pardeep NS, Sabu KK, Abraham TK. 2002. Genetic variation in Pleurotus species (oyster mushrooms) using actual and computer simulated data. Mushroom Research, 11(2): 65-71. https://epubs.icar.org.in/index._php/22579

Pashaei KHA, Irankhah K, Namkhah Z, Sobhani SR. 2024. Edible mushrooms as an alternative to animal proteins for having a more sustainable diet: a review. Journal of Health, Population and Nutrition, 43(205): 1-13. DOI: 10.1186/s41043-024-00701-5

Pathmashini L, Arulnandhy V, Wijeratnam RSW. 2008. Cultivation of oyster mushroom (Pleurotus ostreatus) on sawdust. Ceylon Journal of Science (Biological Sciences), 37(2): 177-182. DOI: 10.4038/cjsbs.v37i2.505

Pathmashini L, Arulnandhy V, Wijeratnam SW. 2008. Cultivation of oyster mushroom (Pleurotus ostreatus) on sawdust. Ceylon Journal of Science (Biological Sciences), 37(2): 177-182. DOI: 10.4038/cjsbs.v37i2.505.

Pedroso ML, Alagos ALC, Esgrina FJO, Habibun JF. 2024. Effect of textural attributes of tree sawdust substrates on Pleurotus oyster mushroom production. The Seybold Report, 19(09): 71-87. DOI: 10.5281/zenodo.13744772

Raghav DK, Singh G, Agarwal NK, Bhati AS, Sharma SK. 2016. Cultivation of oyster mushroom (Pleurotus florida) in North Chotanagpur region of Jharkhand on locally available subtract. The Journal of Rural and Agricultural Research, 16(1): 50-52. https://www.researchgate.net/publication/347508899_Cultivation_of_Oyster_mushroom

Rambey R, Simbolon FM, Siregar EBM. 2020. Growth and productivity of oyster mushrooms (Pleurotus ostreatus) on media rice straw mixed with sawdust. International Conference on Agriculture, Environment and Food Security, 012082. DOI: 10.1088/1755-1315/454/1/012082.

Rashid MH, Bhattacharjya DK, Paul RK, Rahaman MS, Rahaman MS, Miah MN, Ahmed KU. 2016. Effect of different saw dust substrates on the growth and yield of oyster mushroom (Pleurotus florida). Bioresearch Communications, 2(1): 193-199.

https://www.researchgate.net/publication/303519172_Effect_of_Different_Saw_Dust_Substrates_on_the_Growth_and_Yield_of_Oyster_Mushroom_Pleurotus_florida

Riseh RS, Vazvani MG, Hassanisaadi M, Thakur VK. 2024. Agricultural wastes: A practical and potential source for the isolation and preparation of cellulose and application in agriculture and different industries. Industrial Crops and Products, 208, 117904. DOI: 10.1016/j.indcrop.2023.117904

Shah Z, Ashraf M, Ishtiaq M. 2004. Comparative study on cultivation and yield performance of oyster mushroom (Pleurotus ostreatus) on different substrates (wheat straw, leaves and sawdust). Pakistan Journal of Nutrition, 3(3): 158-160. DOI: 10.3923/pjn.2004.158.160.

?lusarczyk J, Adamska E, Czerwik-Marcinkowska J. 2021. Fungi and algae as sources of medicinal and other biologically active compounds: A review. Nutrients, 13(9): 3178. DOI: 10.3390/nu13093178.

Suwannarach N, Kumla J, Zhao Y, Kakumyan P. 2022. Impact of cultivation substrate and microbial community on improving mushroom productivity: a review. Biology, 11(4): 569. DOI: 10.3390/biology11040569

Thomas ASS, Arivu S, Shanmugam V, Gundupalli MP, Amornraksa S. 2022. Paddy straw: An economical substrate for oyster mushroom (Pleurotus ostreatus) cultivation. E3S Web of Conferences, 355: 02019. DOI: 10.1051/e3sconf/202235502019

Tirkey VJ, Simon S, Lal AA. 2017. Efficacy of different substrates on the growth, yield and nutritional composition of oyster mushroom- Pleurotus florida (Mont.) Singer. Journal of Pharmacognosy and Phytochemistry, 6(4): 1097-1100. https://www.phytojournal.com/archives/2017/vol6issue4.pdf

Ulrich JD, Thornsbury S, Court C, Heinzmann AB. 2024. Measuring agriculture research and development return on investment: Relevance for Florida. University of Florida IFAS Extension. DOI: 10.32473/edis-fe1148-2024

Valverde ME, Hernández-Pérez T, Paredes-López O. 2015. Edible mushrooms: Improving human health and promoting quality life. International Journal of Microbiology, 376387. DOI: 10.1155/2015/376387.

Varghese BP, Amritkumar P. 2020. Comparative study on cultivation of oyster mushrooms using nutrition enhancing substrates. International Journal of Scientific Research in Biological Sciences, 7(2): 105-111. https://www.isroset.org/pdf_paper_view._php?paper_id=1864&12-IJSRBS-03340.pdf

Xu Q, Zhang T, Niu Y, Mukherjee S, Abou-Elwafa SF, Nguyen NSH, Al Aboud NM, Wang Y, Pu M, Zhang Y, Tran HT, Almazroui M, Hooda PS, Bolan NS, Rinklebe J, Shaheen SM. 2024. A comprehensive review on agricultural waste utilization through sustainable conversion techniques, with a focus on the additives effect on the fate of phosphorus and toxic elements during composting process. Science of the Total Environment, 942, 173567. DOI: 10.1016/j.scitotenv.2024.173567

Zakil FA, Hassan KHM, Sueb MSM, Isha R. 2020. Growth and yield of Pleurotus ostreatus using sugarcane bagasse as an alternative substrate in Malaysia. IOP Conference Series: Materials Science and Engineering, 736, 022021. DOI:10.1088/1757-899X/736/2/022021

Zamfir M, Manea MD, Ionescu L. 2016. Return on investment – indicator for measuring the profitability of invested capital. Valahian Journal of Economic Studies, 2(7): 79-86. DOI: 10.1515/vjes-2016-0010