Physicochemical properties and antibacterial activity of essential oil fractions obtained from fresh Litsea angulata leaves
Article Sidebar
Abstract Views: 202
File Views: 129
Main Article Content
Abstract
Abstract. Putri AS, Nur M, Kusuma IW, Egra S, Kuspradini H. 2025. Physicochemical properties and antibacterial activity of essential oil fractions obtained from fresh Litsea angulata leaves. Nusantara Bioscience 17: 96-102. Litsea angulata (Lauraceae) is widely grown in Kalimantan Island and is traditionally used as a medicinal plant. This work aimed to analyze the characteristics of the physicochemical from L. angulata oil fractions and the potency of its antibacterial activity. Litsea angulata fresh leaves were distilled, and their oil fractions were obtained by simple fractional distillation using water and steam distillation methods. The fraction of essential oil is based on the different distillation times. In this experiment, the following distillation periods were tested: 0-60 min, 60-120 min, and 120-180 min. The yield and color determined the physicochemical properties, while the chemical properties, as their compositions, were analyzed by GC-MS. The agar diffusion method was employed to assess antibacterial activity. Two kinds of synthetic standards, including chlorhexidine and chloramphenicol, were used to compare their antibacterial properties. Streptococcus sobrinus, Streptococcus mutans and Staphylococcus aureus were the microorganisms used in this investigation. The results showed fractions yield (range 0.09-1.66%) reached a maximum in 0-60 min, and color clearness. All oil fractions have various colors and chemical components according to their treatments. The diameter inhibition against selected bacteria (range 10.22-15.11 mm) was highest at fraction 0-60 min. This essential oil is potentially developed in the pharmaceutical industry as a natural alternative product.
Article Details
Issue
Section
How to Cite
References
Allahverdiyev, A., Duran, N., Ozguven, M., & Koltas, S. (2004). Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2. Phytomedicine, 11(7–8), 657–661. https://doi.org/10.1016/j.phymed.2003.07.014
Arpi, N., Erika, C., & Ermaya, D. (2011). Survey and study on yield and quality of patchouli oil in Aceh Barat Daya District, Indonesia based on original area of raw materials, methods and length of distillation. In Proceedings of The Annual International Conference Syiah Kuala University (Vol. 1, pp. 22–27).
Astani, A., Reichling, J., & Schnitzler, P. (2011). Screening for Antiviral Activities of Isolated Compounds from Essential Oils. Evidence-Based Complementary and Alternative Medicine, 2011(253643), 1–8. https://doi.org/10.1093/ecam/nep187
Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils – A review. Food and Chemical Toxicology, 46(2), 446–475. https://doi.org/10.1016/j.fct.2007.09.106
Baydar, H., Sa?diç, O., Özkan, G., & Karado?an, T. (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. https://doi.org/10.1016/S0956-7135(03)00028-8
Bouazama, S., Harhar, H., Costa, J., Desjobert, J. M., Talbaoui, A., & Tabyaoui, M. (2017). Chemical composition and antibacterial activity of the essential oils of Lavandula pedunculata and Lavandula dentate. Journal of Materials and Environmental Sciences, 8(6), 2154–2160.
Bowles, E. J. (2003). The Chemistry of Aromatherapeutic Oils. Australia: Allen & Unwin. https://doi.org/10.4324/9781003115151
Chang, Y. Te, & Chu, F. H. (2011). Molecular cloning and characterization of monoterpene synthases from Litsea cubeba (Lour.) Persoon. Tree Genetics and Genomes. https://doi.org/10.1007/s11295-011-0377-3
Chaudhari, A. K., Singh, A., Singh, V. K., Dwivedy, A. K., Das, S., Ramsdam, M. G., … Dubey, N. K. (2020). Assessment of chitosan biopolymer encapsulated ?-Terpineol against fungal, aflatoxin B1 (AFB1) and free radicals mediated deterioration of stored maize and possible mode of action. Food Chemistry, 311, 126010. https://doi.org/10.1016/j.foodchem.2019.126010
Chen, J., Tang, C., Zhang, R., Ye, S., Zhao, Z., Huang, Y., … Yang, D. (2020). Metabolomics analysis to eval__uate the antibacterial activity of the essential oil from the leaves of Cinnamomum camphora (Linn.) Presl. Journal of Ethnopharmacology, 253, 112652. https://doi.org/10.1016/j.jep.2020.112652
Del Jiménez-Pérez, D. N. C., Lorea-Hernández, F. G., Jankowski, C. K., & Reyes-Chilpa, R. (2011). Essential Oils in Mexican Bays (Litsea spp., Lauraceae): Taxonomic Assortment and Ethnobotanical Implications1. Economic Botany, 65(2), 178–189. https://doi.org/10.1007/s12231-011-9160-5
Fernandes, E. S., Passos, G. F., Medeiros, R., da Cunha, F. M., Ferreira, J., Campos, M. M., … Calixto, J. B. (2007). Anti-inflammatory effects of compounds alpha-humulene and (?)-trans-caryophyllene isolated from the essential oil of Cordia verbenacea. European Journal of Pharmacology, 569(3), 228–236. https://doi.org/10.1016/j.ejphar.2007.04.059
Flores, C. R., Pennec, A., Nugier-Chauvin, C., Daniellou, R., Herrera-Estrella, L., & Chauvin, A.-L. (2014). Chemical Composition and Antibacterial Activity of Essential Oils Extracted from Plants Cultivated in Mexico Article. J. Mex. Chem. Soc, 58(4), 452–455.
Goyal, S., Tewari, G., Pandey, H. K., & Kumari, A. (2021). Exploration of Productivity, Chemical Composition, and Antioxidant Potential of Origanum vulgare L. Grown at Different Geographical Locations of Western Himalaya, India. Journal of Chemistry, 2021, 1–12. https://doi.org/10.1155/2021/6683300
Hassaballa, L., & Alsiddig, A. (2022). Bio-Taxonomic Study Based on Selected Aromatic Plants Species Related to Different Plant Families as Raw Material. Journal of Food Technology & Nutrition Sciences, 4(5), 1–7. https://doi.org/10.47363/JFTNS/2022(4)152
Herman, R. A., Ayepa, E., Shittu, S., Fometu, S. S., & Wang, J. (2019). Essential Oils and Their Applications -A Mini Review. Advances in Nutrition & Food Science, 4(4). https://doi.org/10.33140/anfs.04.04.08
Hosamath, P. (2011). eval__uation of antimicrobial activity of Litsea Glutinosa. International Journal of Pharmaceutical …, 2(1), 105–114.
Hu, F., Tu, X.-F., Thakur, K., Hu, F., Li, X.-L., Zhang, Y.-S., … Wei, Z.-J. (2019). Comparison of antifungal activity of essential oils from different plants against three fungi. Food and Chemical Toxicology, 134, 110821. https://doi.org/10.1016/j.fct.2019.110821
Ili?, Z., Stanojevi?, L., Milenkovi?, L., Šuni?, L., Milenkovi?, A., Stanojevi?, J., & Cvetkovi?, D. (2022). The Yield, Chemical Composition, and Antioxidant Activities of Essential Oils from Different Plant Parts of the Wild and Cultivated Oregano (Origanum vulgare L.). Horticulturae, 8(11), 1042. https://doi.org/10.3390/horticulturae8111042
Iordache, A., Horj, E., Toma, A., Cozar, O., & Culea, M. (2011). Determination of amino acid composition of two carp species by GC-MS. Asian Journal of Chemistry, 23(11), 4757–4760.
??can, G. (2017). Antibacterial and anticandidal activities of common essential oil constituents. Records of Natural Products, 11(4), 374–388.
I?can, G., Kirimer, N., Demirci, F., Demirci, B., Noma, Y., & Ba?er, K. H. C. (2012). Biotransformation of (-)-(R)-?-phellandrene: Antimicrobial activity of its major metabolite. Chemistry and Biodiversity. https://doi.org/10.1002/cbdv.201100283
Kamle, M., Mahato, D. K., Lee, K. E., Bajpai, V. K., Gajurel, P. R., Gu, K. S., & Kumar, P. (2019). Ethnopharmacological Properties and Medicinal Uses of Litsea cubeba. Plants, 8(6), 150. https://doi.org/10.3390/plants8060150
Kumar, N., Nepali, K., Sapra, S., Bijjem, K. R. V., Kumar, R., Suri, O. P., & Dhar, K. L. (2012). Effect of nitrogen insertion on the antitussive properties of menthol and camphor. Medicinal Chemistry Research, 21(4), 531–537. https://doi.org/10.1007/s00044-011-9560-1
Kuspradini, H., Putri, A. S., & Diana, R. (2020). Toxicity, antioxidant ability and inhibition of oral pathogens by monoterpene-rich essential oil of Litsea angulata Blume. Agriculture and Natural Resources. https://doi.org/10.34044/j.anres.2020.54.2.15
Kuspradini, H., Sinta Putr, A., & Mitsunaga, T. (2018). Chemical Composition, Antibacterial and Antioxidant Activities of Essential Oils of Dryobalanops lanceolata Burck. Leaf. Research Journal of Medicinal Plants, 12(1), 19–25. https://doi.org/10.3923/rjmp.2018.19.25
Kuspradini, H., Wulandari, I., Putri, A. S., Tiya, S. Y., & Kusuma, I. W. (2019). Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts. F1000Research. https://doi.org/10.12688/f1000research.16620.2
Leonhardt, S. D., Zeilhofer, S., Bluthgen, N., & Schmitt, T. (2010). Stingless Bees Use Terpenes as Olfactory Cues to Find Resin Sources. Chemical Senses, 35(7), 603–611. https://doi.org/10.1093/chemse/bjq058
Liska, A., Rozman, V., Kalinovic, I., Ivecic, M., & Balecevic, R. (2010). Contact and fumigant activity of 1,8-cineole, eugenol and camphor against Tribolium castaneum (Herbst). In Proceedings of the 10th International Working Conference on Stored Product Protection (pp. 716–720).
Love, J. N., Sammon, M., & Smereck, J. (2004). Are one or two dangerous? camphor exposure in toddlers. The Journal of Emergency Medicine, 27(1), 49–54. https://doi.org/10.1016/j.jemermed.2004.02.010
Naeem, A., Abbas, T., Ali, T. M., & Hasnain, A. (2018). Essential Oils: Brief Background and Uses. Annals of Short Reports, 1(1), 1006.
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013). Effect of essential oils on pathogenic bacteria. Pharmaceuticals, 6(12), 1451–1474. https://doi.org/10.3390/ph6121451
Nikoli?, B., Miti?-?ulafi?, D., Vukovi?-Ga?i?, B., & Kneževi?-Vuk?evi?, J. (2011). Modulation of genotoxicity and DNA repair by plant monoterpenes camphor, eucalyptol and thujone in Escherichia coli and mammalian cells. Food and Chemical Toxicology, 49(9), 2035–2045. https://doi.org/10.1016/j.fct.2011.05.015
Oliveira, F., Andrade, L., de Sousa, É., & de Sousa, D. (2014). Anti-Ulcer Activity of Essential Oil Constituents. Molecules, 19(5), 5717–5747. https://doi.org/10.3390/molecules19055717
Papajani, V., Haloci, E., Goci, E., Shkreli, R., & Manfredini, S. (2015). eval__uation of Antifungal Activity of Origanum vulgare and Rosmarinus officinalis Essential Oil Before and After Inclusion in ?-Cyclodextrine. International Journal of Pharmacy and Pharmaceutical Sciences, 7(5), 270–273.
Patty, D. J., & Loupatty, G. (2016). Analysis Of Eucalyptus Oil Distillates Traditionally. Biological and Chemical Research, 295–302.
Phelan, W. J. (1976). Camphor Poisoning: Over-the-Counter Dangers. Pediatrics, 57(3), 428–431. https://doi.org/10.1542/peds.57.3.428
Porter, N. G., Shaw, M. L., & Hurndell, L. C. (1982). Preliminary studies of lavender as an essential oil crop for New Zealand. New Zealand Journal of Agricultural Research, 25(3), 389–394. https://doi.org/10.1080/00288233.1982.10417902
Rabl, W., Katzgraber, F., & Steinlechner, M. (1997). Camphor ingestion for abortion (case report). Forensic Science International, 89(1–2), 137–140. https://doi.org/10.1016/S0379-0738(97)00099-6
Robi, A. J., George, S., & Thushar, K. V. (2015). Litsea udayanii (Lauraceae): A new species from the southern western Ghats, India. Phytotaxa, 222(1), 44–50. https://doi.org/10.11646/phytotaxa.222.1.4
Sell, C. S. (2006). The chemistry of fragrances?: from perfumer to consumer (2nd editio). Cambridge, UK: The Royal Society of Chemistry.
Shaaban, H. A. E., El-Ghorab, A. H., & Shibamoto, T. (2012). Bioactivity of essential oils and their volatile aroma components: Review. Journal of Essential Oil Research, 24(2), 203–212. https://doi.org/10.1080/10412905.2012.659528
Shabbir, M. K., Nadeem, R., Mukhtar, H., Anwar, F., & Mumtaz, M. W. (2009). Physico-chemical analysis and determination of various chemical constituents of essential oil in Rosa centifolia. Pakistan Journal of Botany, 41(2), 615–620.
Shahin, S. M., Jaleel, A., & Alyafei, M. A. M. (2021). The Essential Oil-Bearing Plants in the United Arab Emirates (UAE): An Overview. Molecules, 26(21), 6486. https://doi.org/10.3390/molecules26216486
Su, P. W., Yang, C. H., Yang, J. F., Su, P. Y., & Chuang, L. Y. (2015). Antibacterial activities and antibacterial mechanism of polygonum cuspidatum extracts against nosocomial drug-resistant pathogens. Molecules. https://doi.org/10.3390/molecules200611119
Tanaka, H., Yatsuhashi, S., Yasuda, T., Sato, M., Sakai, E., Xiao, C., … Murata, J. (2009). A new amide from the leaves and twigs of Litsea auriculata. Journal of Natural Medicines. https://doi.org/10.1007/s11418-009-0323-y
Teixeira, B., Marques, A., Ramos, C., Neng, N. R., Nogueira, J. M. F., Saraiva, J. A., & Nunes, M. L. (2013). Chemical composition and antibacterial and antioxidant properties of commercial essential oils. Industrial Crops and Products, 43, 587–595. https://doi.org/10.1016/j.indcrop.2012.07.069
Thangaleela, S., Sivamaruthi, B. S., Kesika, P., Tiyajamorn, T., Bharathi, M., & Chaiyasut, C. (2022). A Narrative Review on the Bioactivity and Health Benefits of Alpha-Phellandrene. Scientia Pharmaceutica, 90(4), 57. https://doi.org/10.3390/scipharm90040057
van Den Dool, H., & Dec. Kratz, P. (1963). A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. Journal of Chromatography A. https://doi.org/10.1016/s0021-9673(01)80947-x
Wang, W., Li, D., Huang, X., Yang, H., Qiu, Z., Zou, L., … Li, Y. (2019). Study on Antibacterial and Quorum-Sensing Inhibition Activities of Cinnamomum camphora Leaf Essential Oil. Molecules, 24(20), 3792. https://doi.org/10.3390/molecules24203792
Wesolowska, A., Jadczak, D., & Grzeszczuk, M. (2010). Influence of distillation time on the content and composition of essential oil isolated from lavender (Lavandula angustifolia Mill.). Herba Polonica, 56(3), 24–36.
Wong, M. H., Lim, L. F., Ahmad, F. bin, & Assim, Z. bin. (2014). Antioxidant and antimicrobial properties of Litsea elliptica Blume and Litsea resinosa Blume (Lauraceae). Asian Pacific Journal of Tropical Biomedicine, 4(5), 386–392. https://doi.org/10.12980/APJTB.4.2014C1129
Zeedan, G. S. G., Abdalhamed, A. M., Ottai, M. E., Shoby-Abdelshafy, & Abdeen, E. (2014). Antimicrobial, Antiviral Activity and GC-MS Analysis of Essential Oil Extracted from Achillea fragrantissima Plant Growing In Sinai Peninsula, Egypt. Journal of Microbial & Biochemical Technology, S8(006), 1–7. https://doi.org/10.4172/1948-5948.s8-006
Zhang, J. hong, Sun, H. long, Chen, S. yang, Zeng, L. I., & Wang, T. tao. (2017). Anti-fungal activity, mechanism studies on ?-Phellandrene and Nonanal against Penicillium cyclopium. Botanical Studies, 58(13), 1–9. https://doi.org/10.1186/s40529-017-0168-8
Zheljazkov, V. D., Astatkie, T., & Schlegel, V. (2014). Hydrodistillation extraction time effect on essential oil yield, composition, and bioactivity of coriander oil. Journal of Oleo Science. https://doi.org/10.5650/jos.ess14014