Development of anti-obesity herbal drink from butterfly pea flower (Clitoria ternatea) extract and lemon (Citrus limon) juice
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Abstract. Anugrahani AD, Indarto D, Pamungkasari EP, Wijayanti L, Utami F. 2025. Development of anti-obesity herbal drink from butterfly pea flower (Clitoria ternatea) extract and lemon (Citrus limon) juice. Nusantara Bioscience 17: 129-136. Obesity is a multifactorial disease with a high global prevalence and higher risk for cardiometabolic diseases. Obesity management comprises diet, physical exercise, and pharmacotherapy. Some obese patients have low compliance with diet and exercise therapy, while long-term use of anti-obesity drugs causes adverse side effects. Herbal drinks have also been used for obesity treatment, but their efficacy remains debatable. Herbal drink products have been developed, including butterfly pea flower extract and lemon juice, which contain flavonoids and anthocyanins that have anti-obesity activity. The purpose of this study was to create a herbal drink for obesity treatment and analyze acceptability, microbiological contamination, and nutritional and phytochemical contents. An organoleptic test assessed the acceptability of the herbal drink. Analysis of macronutrient content used proximate methods. Vitamin C and microbiological tests were performed using spectrophotometric and Total Plate Count (TPC) methods. Delphinidin and hesperidin levels were measured using a High-Performance Liquid Chromatography (HPLC) method. Statistical analysis was performed using Kruskal-Wallis and followed by the post-hoc Mann-Whitney with p<0.05 to determine the best formula of the four formulas (F1-F4) in this study. Organoleptic test results showed that formulation F3 of the herbal drink was the most liked and acceptable by the panelists. The herbal drink (F3) contained 0.82% fat, 0.34% protein, 0.099% carbohydrate, 3,527.25 mg/100 g vitamin C, 21.66 ppm delphinidin, and 525.68 ppm hesperidin. In conclusion, herbal drink has the potential to become an alternative anti-obesity treatment. Further research is needed to test the effectiveness of herbal drinks on weight loss and lipid profile improvement in obese patients.
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Alfaifi FF, Alghamdi AM, Al-omarani B, Dawaji EH, Aljabry H and Al-Gayyar MM (2020). Comprehensive Study of Scientific Evidence and Potential Risk of Herbal Medicine Use for Body Weight Reduction in North West Saudi Arabia. Cureus, 12(10). doi: 10.7759/cureus.10903.
Brandfon S, Eylon A, Khanna D and Parmar MS (2023). Advances in Anti-Obesity Pharmacotherapy: Current Treatments, Emerging Therapies, and Challenges. Cureus, 15(10). doi: 10.7759/cureus.46623.
Büyüktuncel E (2017). Fast Determination of Naringin and Hesperidin in Natural and Commercial Citrus Juices by HPLC Method. Asian Journal of Chemistry, 29(11), pp.: 2384–2386. doi: 10.14233/ajchem.2017.20675.
Chatelan A, Raeisi-Dehkordi H and Salehi-Abargouei A (2024). Substituting Low-Calorie Sweetened Beverages for Sugar-Sweetened Beverages to Prevent Obesity and Cardiometabolic Diseases: Still a Good Idea? Current Developments in Nutrition, 8(3), pp.: 102105. doi: 10.1016/j.cdnut.2024.102105.
Daveri E, Cremonini E, Mastaloudis A, Hester SN, Wood SM, Waterhouse AL, Anderson M, Fraga CG, et al. (2018). Cyanidin and Delphinidin Modulate Inflammation and Altered Redox Signaling Improving Insulin Resistance in High Fat-Fed Mice. Redox Biology, 18(April), pp.: 16–24. doi: 10.1016/j.redox.2018.05.012.
Djekic I, Lorenzo JM, Munekata PES, Gagaoua M and Tomasevic I (2021). Review on Characteristics of Trained Sensory Panels in Food Science. Journal of Texture Studies, 52(4), pp.: 501–509. doi: 10.1111/jtxs.12616.
Franco-San Sebastián D, Alaniz-Monreal S, Rabadán-Chávez G, Vázquez-Manjarrez N, Hernández-Ortega M and Gutiérrez-Salmeán G (2023). Anthocyanins: Potential Therapeutic Approaches towards Obesity and Diabetes Mellitus Type 2. Molecules, 28(3). doi: 10.3390/molecules28031237.
Hardinsyah H, Gunawan W Ben, Nurkolis F, Alisaputra D, Kurniawan R, Mayulu N, Taslim NA and Tallei TE (2023). Antiobesity Potential of Major Metabolites from Clitoria Ternatea Kombucha: Untargeted Metabolomic Profiling and Molecular Docking Simulations. Current Research in Food Science, 6(November 2022). doi: 10.1016/j.crfs.2023.100464.
Imessaoudene A, Merzouk AZ, Guermouche B, Merzouk H and Merzouk SA (2022). In Vitro Effects of Vitamins C and E on Adipocyte Function and Redox Status in Obesity. PharmaNutrition, 22(July), pp.: 100315. doi: 10.1016/j.phanu.2022.100315.
Jamar G, Santamarina AB, Flygare AC, Gagliardi A, de Rosso VV, Dourado VZ and Pisani LP (2020). Effects of the Juçara Fruit Supplementation on Metabolic Parameters in Individuals with Obesity: A Double-Blind Randomized Controlled Trial. Journal of Nutritional Biochemistry, 83, pp.: 108430. doi: 10.1016/j.jnutbio.2020.108430.
Keramat SA, Alam K, Al-Hanawi MK, Gow J, Biddle SJH and Hashmi R (2021). Trends in the Preval__ence of Adult Overweight and Obesity in Australia, and Its Association with Geographic Remoteness. Scientific Reports, 11(1), pp.: 1–9. doi: 10.1038/s41598-021-90750-1.
Khan NH, Abdulbaqi IM, Darwis Y, Aminu N and Chan SY (2022). A Stability-Indicating HPLC-UV Method for the Quantification of Anthocyanin in Roselle (Hibiscus Sabdariffa L.) Spray-Dried Extract, Oral Powder, and Lozenges. Heliyon, 8(3). doi: 10.1016/j.heliyon.2022.e09177.
Kim JY (2021). Optimal Diet Strategies for Weight Loss and Weight Loss Maintenance. Journal of Obesity and Metabolic Syndrome, 30(1), pp.: 20–31. doi: 10.7570/JOMES20065.
Lin Y, Shi D, Su B, Wei J, G?man MA, Sedanur Macit M, Borges do Nascimento IJ and Guimaraes NS (2020). The Effect of Green Tea Supplementation on Obesity: A Systematic Review and Dose–Response Meta-Analysis of Randomized Controlled Trials. Phytotherapy Research, 34(10), pp.: 2459–2470. doi: 10.1002/ptr.6697.
Markowiak-Kope? P and ?li?ewska K (2020). The Effect of Probiotics on the Production of Short-Chain Fatty Acids by Human Intestinal Microbiome. Nutrients, 12(4). doi: 10.3390/nu12041107.
Noguera (2021). 8 Weeks of Hesperidin Supplementation Improves Muscle Mass and Reduces Fat in Amateur Competitive Cyclist-RCT, pp.: 1–11.
Park M, Sharma A and Lee HJ (2019). Anti-Adipogenic Effects of Delphinidin-3-O-?-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes. Molecules, 24(10), pp.: 1–12. doi: 10.3390/molecules24101848.
Park SJ, Sharma A and Lee HJ (2023). Postbiotics against Obesity: Perception and Overview Based on Pre-Clinical and Clinical Studies. International Journal of Molecular Sciences, 24(7). doi: 10.3390/ijms24076414.
Peteliuk V, Rybchuk L, Bayliak M, Storey KB and Lushchak O (2021). Natural Sweetener Stevia Rebaudiana: Functionalities, Health Benefits and Potential Risks. EXCLI Journal, 20, pp.: 1412–1430. doi: 10.17179/excli2021-4211.
Powell-Wiley TM, Poirier P, Burke LE, Després JP, Gordon-Larsen P, Lavie CJ, Lear SA, Ndumele CE, et al. (2021). Obesity and Cardiovascular Disease A Scientific Statement From the American Heart Association. Circulation, 143(21), pp.: E984–E1010. doi: 10.1161/CIR.0000000000000973.
Raghavan G, Bapna A, Mehta A, Shah A and Vyas T (2023). Effect of Sugar Replacement with Stevia-Based Tabletop Sweetener on Weight and Cardiometabolic Health among Indian Adults. Nutrients, 15(7). doi: 10.3390/nu15071744.
Ruiz-Capillas C and Herrero AM (2021). Sensory Analysis and Consumer Research in New Product Development. Foods, 10(3), pp.: 2–5. doi: 10.3390/foods10030582.
Sari FYK, Damayanthi E and Kustiyah L (2018). The Effect of Rosella Beverage Intervention on Lipid Profileand Antropometric in Obese Adult Men. Indian Journal of Public Health Research and Development, 9(12), pp.: 644–649. doi: 10.5958/0976-5506.2018.01910.1.
Saulite L, Jekabsons K, Klavins M, Muceniece R and Riekstina U (2019). Effects of Malvidin, Cyanidin and Delphinidin on Human Adipose Mesenchymal Stem Cell Differentiation into Adipocytes, Chondrocytes and Osteocytes. Phytomedicine, 53, pp.: 86–95. doi: 10.1016/j.phymed.2018.09.029.
Sultana S, Rahman MM, Sigel B and Hashizume M (2021). Associations of Lifestyle Risk Factors with Overweight or Obesity among Adolescents: A Multicountry Analysis. American Journal of Clinical Nutrition, 113(3), pp.: 742–750. doi: 10.1093/ajcn/nqaa337.
Tak YJ and Lee SY (2021). Long-Term Efficacy and Safety of Anti-Obesity Treatment: Where Do We Stand? Current Obesity Reports, 10(1), pp.: 14–30. doi: 10.1007/s13679-020-00422-w.
Trujillo-Garrido N and Santi-Cano MJ (2022). Motivation and Limiting Factors for Adherence to Weight Loss Interventions among Patients with Obesity in Primary Care. Nutrients, 14(14), pp.: 1–11. doi: 10.3390/nu14142928.
Utami F, Indarto D and Listyawati S (2023). Enhancement of Flavonoid and Anthocyanin Levels of Herbal Beverage Containing Butterfly Pea Flower and Lemon Fruit to Improve Anti-Oxidant Activity, (Icsdh 2022), pp.: 53–59. doi: 10.5220/0011642600003608.
Valeeva F V., Medvedeva MS, Khasanova KB, Valeeva E V., Kiseleva TA, Egorova ES, Pickering C and Ahmetov II (2022). Association of Gene Polymorphisms with Body Weight Changes in Prediabetic Patients. Molecular Biology Reports, 49(6), pp.: 4217–4224. doi: 10.1007/s11033-022-07254-y.
Wici?ski M, G?balski J, Go??biewski J and Malinowski B (2020). Probiotics for the Treatment of Overweight and Obesity in Humans—a Review of Clinical Trials. Microorganisms, 8(8), pp.: 1–26. doi: 10.3390/microorganisms8081148.
Widowati W, Wargasetia TL, Zakaria TM, Marthania M, Akbar RATPP, Gunadi MS, Halim N and Santiadi S (2022). Antioxidant Activity of TEMON (Clitoria Ternatea and Citrus Sp.) as an Infused Herbal Tea. Majalah Obat Tradisional, 27(1), pp.: 32–40. doi: 10.22146/mot.71628.
Xiong H (2019). Hesperidin: A Therapeutic Agent For Obesity. Dove Press Journal, 13(Drug Design, Development and Therapy), pp.: 3855–3866.