Stomatal anatomical variation of water hyacinth (Pontederia crassipes) across Bengawan Solo Oxbow Environments, Indonesia
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Abstract. Zagoto JR, Febrisa KN, Mahmudah IR, Qanita IM, Rifki AN, Setyawan AD. 2025. Stomatal anatomical variation of water hyacinth (Pontederia crassipes) across Bengawan Solo Oxbow Environments, Indonesia. Cell Biol Dev 9: 99-112. Oxbow lakes formed along large tropical rivers represent heterogeneous lentic habitats that can impose contrasting environmental conditions on aquatic macrophytes. This study examined stomatal anatomical variation of water hyacinth (Pontederia crassipes) across three Bengawan Solo Oxbow Environments in Central Java, Indonesia, and evaluated how these anatomical traits relate to supporting physiological and growth indicators. Stomatal characteristics were quantified using a leaf-impression method on adaxial and abaxial leaf surfaces, while environmental parameters were measured in situ. Chlorophyll status was assessed using a Soil Plant Analysis Development (SPAD) Index, and plant performance was evaluated through dry biomass measurement. Across all oxbows, P. crassipes exhibited an amphistomatous leaf condition with anomocytic stomatal complexes, indicating a conserved stomatal structural pattern consistent with its free-floating growth form. Within this shared framework, quantitative variation in stomatal size and density distinguished oxbow environments. Tangkisan showed the highest stomatal densities on both leaf surfaces, whereas Kadokan exhibited the lowest values, with Sidowarno occupying an intermediate position. SPAD chlorophyll index and dry weight also differed among oxbows but did not correspond directly with stomatal traits, demonstrating that physiological status and biomass accumulation are influenced by multiple interacting environmental factors rather than stomatal anatomy alone. By integrating stomatal anatomy with SPAD and biomass measurements, this study highlights stomatal traits as robust anatomical indicators of environmental context in oxbow habitats, while emphasizing the role of physiological and growth metrics as supporting, not primary, indicators. The findings provide an anatomically grounded framework for comparative assessment of plant-habitat interactions in heterogeneous oxbow environments and underscore the potential of stomatal anatomy as a practical, field-relevant tool for evaluating habitat assessment in tropical river systems.
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References
Ajithram A, Jappes JW, Brintha NC. 2021. Water hyacinth (Pontederia crassipes) natural composite extraction methods and properties: A review. Mater Today Proc 45: 1626-1632. DOI: 10.1016/j.matpr.2020.08.472.
Ali H, Khan E, Sajad MA. 2013. Phytoremediation of heavy metals-Concepts and applications. Chemosphere 91 (7): 869-881. DOI: 10.1016/j.chemosphere.2013.01.075.
Ayunin Q, Mulyani I. 2023. Diversity and percent cover of aquatic plants in Parit Lake, Tambang District, Kampar Regency, Riau Province. Berkala Perikanan Terubuk 51 (2): 1882-1889. DOI: 10.31258/terubuk.51.1.1828-1834.
Badger MR, Price GD. 1994. The role of carbonic anhydrase in photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 45: 369-392. DOI: 10.1146/annurev.pp 45.060194.002101.
Buckley TN, John GP, Scoffoni C, Sack L. 2015. How does leaf anatomy influence water transport outside the xylem? Plant Physiol 168 (4): 1616-1635. DOI: 10.1104/pp.15.00731.
Dow GJ, Berry JA, Bergmann DC. 2014. The physiological importance of developmental mechanisms that enforce proper stomatal spacing in Arabidopsis thaliana. New Phytol 201 (4): 1205-1217. DOI: 10.1111/nph.12586.
Driesen E, Van den Ende W, De Proft M, Saeys W. 2020. Influence of environmental factors, light, CO₂, temperature, and relative humidity, on stomatal opening and development: A review. Agronomy 10 (12): 1975. DOI: 10.3390/agronomy10121975.
Fahruddin, Borrong T, Tanjung RE, Abdullah A, Tuwo M. 2023. Physical changes of hyacinth Eichhornia crassipes in heavy metal phytoremediation. Jurnal Ilmu Alam dan Lingkungan 14 (1): 65-71. DOI: 10.20956/jal.v14i1.26251. [Indonesian]
Febjislami S, Hasibuan SP. 2023. Optimization and modification of the stomata collection method of the long bean plant (Vigna sesquipedalis) using the stomatal printing method. Jurnal Pertanian Presisir 7 (1): 59-73. DOI: 10.35760/jpp.2023.v7i1.8275. [Indonesian]
Flexas J, Díaz-Espejo A, Conesa MA, Coopman RE, Douthe C, Gago J, Gallé A, Galmés J, Medrano H, Ribas-Carbo M, Tomàs M, Niinemets Ü. 2016. Mesophyll conductance to CO₂ and Rubisco as targets for improving intrinsic water use efficiency in C₃ plants. Plant Cell Environ 39 (5): 965-982. DOI: 10.1111/pce.12622.
Franks PJ, Beerling DJ. 2009. Maximum leaf conductance driven by CO₂ effects on stomatal size and density over geologic time. Proc Natl Acad Sci USA 106 (25): 10343-10347. DOI: 10.1073/pnas.0904209106.
Franks PJ, Farquhar GD. 2007. The mechanical diversity of stomata and its significance in gas-exchange control. Plant Physiol 143 (1): 78-87. DOI: 10.1104/pp.106.089367.
Gultom EY, Ardianor A, Gumiri S, Handayani T. 2023. Jenis dan kelimpahan zooplankton yang berenang bebas dan terlepas dari perakaran eceng gondok (Pontederia crassipes), kiambang (Salvinia natans), dan apu-apu (Pistia stratiotes) di zona interrhizone. J Trop Fish 18 (2): 51-59. DOI: 10.36873/jtf.v18i2.11113. [Indonesian]
Hanafiyanto F, Wahyono. 2021. Comparison of measurement accuracy of chlorophyll and nitrogen levels between SPAD and NDVI in corn (Zea mays). Jurnal Agro Indragiri 8 (2): 11-21. DOI: 10.32520/jai.v8i2.1747. [Indonesian]
Hetherington AM, Woodward FI. 2003. The role of stomata in sensing and driving environmental change. Nature 424: 901-908. DOI: 10.1038/nature01843.
Hurlbert SH. 1984. Pseudoreplication and the design of ecological field experiments. Ecol Monogr 54: 187-211. DOI: 10.2307/1942661.
Izzati H, Rustandi I. 2025. Normalization of the Citarum dead river corridor landscape with hydrological analysis (Case study: Rancamanyar Village, Bandung Regency). Teknik Sipil dan Arsitektur 30 (1): 32-41. DOI: 10.36728/jtsa.v30i1.4153. [Indonesian]
Lambers H, Chapin FS, Pons TL. 2008. Plant Physiological Ecology. 2nd ed. Springer, New York. DOI: 10.1007/978-0-387-78341-3.
Lawson T, Vialet-Chabrand S. 2019. Speedy stomata, photosynthesis, and plant water use efficiency. New Phytol 221 (1): 93-98. DOI: 10.1111/nph.15330.
Maberly SC, Spence DHN. 1983. Photosynthetic inorganic carbon use by freshwater plants. J Ecol 71 (3): 705-724. DOI: 10.2307/2259587.
Mahmood Q, Zheng P, Siddiqi MR, Islam EU, Azim MR, Hayat Y. 2005. Anatomical studies on water hyacinth (Eichhornia crassipes (Mart.) Solms) under the influence of textile wastewater. J Zhejiang Univ Sci B 6 (10): 991-998. DOI: 10.1631/jzus.2005.B0991.
McElwain JC, Yiotis C, Lawson T. 2016. Using modern plant trait relationships between observed and theoretical maximum stomatal conductance and vein density to examine patterns of plant macroevolution. New Phytol 209 (1): 94-103. DOI: 10.1111/nph.13579.
Metcalfe CR, Chalk L. 1979. Anatomy of the Dicotyledons Vol. 1: Systematic Anatomy of the Leaf and Stem. Oxford University Press, New York.
Mommer L, Pons TL, Visser EJW. 2006. Photosynthetic consequences of phenotypic plasticity in response to submergence: Rumex palustris as a case study. J Exp Bot 57 (2): 283-290. DOI: 10.1093/jxb/erj015.
Mott KA, Gibson AC, O’Leary JW. 1982. The adaptive significance of amphistomatic leaves. Plant Cell Environ 5 (6): 455-460. DOI: 10.1111/1365-3040.ep11611750.
Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Ann Rev Plant Biol 59: 651-681. DOI: 10.1146/annurev.arplant.59.032607.092911.
Murchie EH, Niyogi KK. 2011. Manipulation of photoprotection to improve plant photosynthesis. Plant Physiol 155 (1): 86-92. DOI: 10.1104/pp.110.168831.
Ningrum YD, Ghofar A, Haeruddin H. 2020. Effectiveness of hyacinths (Pontederia crassipes (Mart.) Solms) as a phytoremediator for tofu production liquid waste. Manag Aquat Res J (MAQUARES) 9 (2): 97-106. DOI: 10.14710/marj.v9i2.27765. [Indonesian]
Parkhurst DF, Loucks OL. 1972. Optimal leaf size in relation to the environment. J Ecol 60 (2): 505-537. DOI: 10.2307/2258359.
Parkhurst DF. 1978. The adaptive significance of stomatal occurrence on one or both surfaces of leaves. J Ecol 66 (2): 367-383. DOI: 10.2307/2259142.
Poorter H, Niklas KJ, Reich PB, Oleksyn J, Poot P, Mommer L. 2012. Biomass allocation to leaves, stems, and roots: Meta-analyses of interspecific variation and environmental control. New Phytol 193 (1): 30-50. DOI: 10.1111/j.1469-8137.2011.03952.x.
Prastika D, Sarjani TM, Mahyuni SR, Hariani I, Ramadhan DA, Rezeki S, Tiara R, Hendrik E, Aulia R, Amalia T. 2023. Identification of stomata types of Myrtaceae tribe members in Langsa City. Jurnal Sains dan Edukasi Sains 6 (1): 20-27. DOI: 10.24246/juses.v6i1p20-27.
Qodriyah L, Wahidah BF, Hidayat S, Khasanah R. 2021. Characterization of leaf stomata in ornamental plants of the Araceae family. In: Prosiding Seminar Biologi 7 (1): 242-249. DOI: 10.24252/psb.v7i1.24241. [Indonesian]
Rai PK. 2016. Impacts of particulate matter pollution on plants: Implications for environmental biomonitoring. Ecotoxicol Environ Saf 129: 120-136. DOI: 10.1016/j.ecoenv.2016.03.012.
Rascio N. 2002. The underwater life of secondarily aquatic plants: Some problems and solutions. Crit Rev Plant Sci 21 (4): 401-427. DOI: 10.1080/0735-260291044296.
Saputra AK, Santoso DH, Yudono ARA. 2020. Zoning level of flood vulnerability on the former river in Sukoharjo Regency. Jurnal Geografi 12 (1): 255-261. DOI: 10.24114/jg.v12i01.14390. [Indonesian]
Schneider CA, Rasband WS, Eliceiri KW. 2012. NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9 (7): 671-675. DOI: 10.1038/nmeth.2089.
Sculthorpe CD. 1967. The Biology of Aquatic Vascular Plants. Edwards Arnold, London.
Shtein I, Popper ZA, Harpaz-Saad S. 2017. Permanently open stomata of aquatic angiosperms display modified cellulose crystallinity patterns. Plant Signal Behav 12 (7): e1339858. DOI: 10.1080/15592324.2017.1339858.
Sopiana SR, Hermanto, Nur EA. 2022. The effect of swallow manure fertilizer on the growth of Liberica coffee seedlings (Coffea liberica) in peat media. J Agro Plantation 1 (2): 74-84. DOI: 10.58466/jap.v1i2.1242.
Sumadji AR, Purwaningsih CE, Ganjari LE. 2023. Stomata characteristics of the breadfruit plant Artocarpus altilis (Park.) Fosberg in Bekasi City. Prosiding Seminar Nasional Biologi 3 (2): 73-82. [Indonesian]
Suraya U. 2019. Inventarisasi dan identifikasi tumbuhan air di Danau Hanjalutung Kota Palangka Raya. Daun Jurnal Ilmiah Pertanian dan Kehutanan 6 (2): 149-159. DOI: 10.33084/daun.v6i2.1261. [Indonesian]
Taiz L, Zeiger E, Møller IM, Murphy A. 2015. Plant Physiology and Development. Sinauer Associates, Sunderland, USA.
Uddling J, Gelang-Alfredsson J, Piikki K, Pleijel H. 2007. Evaluating the relationship between leaf chlorophyll concentration and SPAD-502 readings. Photosynth Res 91: 37-46. DOI: 10.1007/s11120-006-9077-5.
Villamagna A, Murphy B. 2010. Ecological and socio-economic impacts of invasive water hyacinth (Eichhornia crassipes): A review. Freshw Biol 55: 282-298. DOI: 10.1111/j.1365-2427.2009.02294.x.
Wetzel RG. 2001. Limnology: Lake and River Ecosystems. 3rd ed. Academic Press, San Diego.
Windarsih G, Riastiwi I, Dewi A, Yuriyah S. 2022. Stomatal and epidermal characteristics of Zingiberaceae in Serang District, Banten, Indonesia. Biodiversitas 23: 5373-5386. DOI: 10.13057/biodiv/d231048.